(Linear) (Six-base) MAPSORT of: KIEE4376.seq Check: 3887 from: 1 to: 1197 KIEE4376.orf With 182 enzymes: SgfI * July 3, 2012 19:00 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 376 445 514 583 652 721 1170 1197 Size: 376 69 69 69 69 69 449 27 Fragments arranged by size: 449 376 69 69 69 69 69 27 AlwNI CAG_nnn'CTG Cuts at: 0 33 276 345 414 483 552 621 690 Size: 33 243 69 69 69 69 69 69 Cuts at: 690 1124 1197 Size: 434 73 Fragments arranged by size: 434 243 73 69 69 69 69 69 69 33 ApaI G_GGCC'C Cuts at: 0 903 1197 Size: 903 294 ApoI r'AATT_y Cuts at: 0 1108 1197 Size: 1108 89 BanI G'GyrC_C Cuts at: 0 145 1197 Size: 145 1052 BanII G_rGCy'C Cuts at: 0 124 308 903 1197 Size: 124 184 595 294 Fragments arranged by size: 595 294 184 124 BbeI G_GCGC'C Cuts at: 0 149 1197 Size: 149 1048 BbsI GAAGACnn'nnnn_ Cuts at: 0 778 1103 1197 Size: 778 325 94 Fragments arranged by size: 778 325 94 BbvCI CC'TCA_GC Cuts at: 0 926 1197 Size: 926 271 BglII A'GATC_T Cuts at: 0 332 401 470 539 608 677 1197 Size: 332 69 69 69 69 69 520 Fragments arranged by size: 520 332 69 69 69 69 69 Bme1580I G_kGCm'C Cuts at: 0 903 1197 Size: 903 294 Bpu10I CC'TnA_GC Cuts at: 0 926 1197 Size: 926 271 BsaAI yAC'GTr Cuts at: 0 1013 1197 Size: 1013 184 BsaHI Gr'CG_yC Cuts at: 0 146 1197 Size: 146 1051 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 958 988 1197 Size: 958 30 209 Fragments arranged by size: 958 209 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 154 943 1197 Size: 154 789 254 Fragments arranged by size: 789 254 154 BseYI C'CCAG_C Cuts at: 0 33 184 226 281 350 695 854 902 Size: 33 151 42 55 69 345 159 48 Cuts at: 902 1045 1197 Size: 143 152 Fragments arranged by size: 345 159 152 151 143 69 55 48 42 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 1197 Size: 46 1151 BsiHKAI G_wGCw'C Cuts at: 0 124 308 1197 Size: 124 184 889 Fragments arranged by size: 889 184 124 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1184 1197 Size: 1184 13 Bsp1286I G_dGCh'C Cuts at: 0 124 308 903 1197 Size: 124 184 595 294 Fragments arranged by size: 595 294 184 124 BsrDI GCAATG_nn' Cuts at: 0 1024 1197 Size: 1024 173 BstYI r'GATC_y Cuts at: 0 51 332 401 470 539 608 677 1160 Size: 51 281 69 69 69 69 69 483 Cuts at: 1160 1197 Size: 37 Fragments arranged by size: 483 281 69 69 69 69 69 51 37 BtgI C'CryG_G Cuts at: 0 141 1197 Size: 141 1056 BtsI GCAGTG_nn' Cuts at: 0 302 371 440 509 578 647 716 1125 Size: 302 69 69 69 69 69 69 409 Cuts at: 1125 1197 Size: 72 Fragments arranged by size: 409 302 72 69 69 69 69 69 69 ClaI AT'CG_AT Cuts at: 0 1137 1197 Size: 1137 60 EaeI y'GGCC_r Cuts at: 0 111 138 197 1197 Size: 111 27 59 1000 Fragments arranged by size: 1000 111 59 27 EcoICRI GAG'CTC Cuts at: 0 122 306 1197 Size: 122 184 891 Fragments arranged by size: 891 184 122 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 376 445 514 583 652 721 1170 1197 Size: 376 69 69 69 69 69 449 27 Fragments arranged by size: 449 376 69 69 69 69 69 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 238 1000 1197 Size: 238 762 197 Fragments arranged by size: 762 238 197 EcoO109I rG'GnC_Cy Cuts at: 0 189 222 899 1197 Size: 189 33 677 298 Fragments arranged by size: 677 298 189 33 HaeII r_GCGC'y Cuts at: 0 149 1197 Size: 149 1048 HincII GTy'rAC Cuts at: 0 1149 1197 Size: 1149 48 KasI G'GCGC_C Cuts at: 0 145 1197 Size: 145 1052 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 79 1197 Size: 79 1118 MscI TGG'CCA Cuts at: 0 113 140 199 1197 Size: 113 27 59 998 Fragments arranged by size: 998 113 59 27 MspA1I CmG'CkG Cuts at: 0 33 354 699 858 948 1056 1197 Size: 33 321 345 159 90 108 141 Fragments arranged by size: 345 321 159 141 108 90 33 NarI GG'CG_CC Cuts at: 0 146 1197 Size: 146 1051 NcoI C'CATG_G Cuts at: 0 141 1197 Size: 141 1056 PflMI CCAn_nnn'nTGG Cuts at: 0 812 1197 Size: 812 385 PfoI T'CCnGG_A Cuts at: 0 41 958 976 1197 Size: 41 917 18 221 Fragments arranged by size: 917 221 41 18 PmlI CAC'GTG Cuts at: 0 1013 1197 Size: 1013 184 PpuMI rG'GwC_Cy Cuts at: 0 189 222 1197 Size: 189 33 975 Fragments arranged by size: 975 189 33 PspOMI G'GGCC_C Cuts at: 0 899 1197 Size: 899 298 PstI C_TGCA'G Cuts at: 0 297 329 366 398 435 467 504 536 Size: 297 32 37 32 37 32 37 32 Cuts at: 536 573 605 642 674 711 953 1197 Size: 37 32 37 32 37 242 244 Fragments arranged by size: 297 244 242 37 37 37 37 37 37 32 32 32 32 32 32 PvuII CAG'CTG Cuts at: 0 33 354 699 948 1197 Size: 33 321 345 249 249 Fragments arranged by size: 345 321 249 249 33 SacI G_AGCT'C Cuts at: 0 124 308 1197 Size: 124 184 889 Fragments arranged by size: 889 184 124 SfcI C'TryA_G Cuts at: 0 125 293 325 362 394 431 463 500 Size: 125 168 32 37 32 37 32 37 Cuts at: 500 532 569 601 638 670 707 943 949 Size: 32 37 32 37 32 37 236 6 Cuts at: 949 1197 Size: 248 Fragments arranged by size: 248 236 168 125 37 37 37 37 37 37 32 32 32 32 32 32 6 SfoI GGC'GCC Cuts at: 0 147 1197 Size: 147 1050 StyI C'CwwG_G Cuts at: 0 11 19 141 218 806 825 1197 Size: 11 8 122 77 588 19 372 Fragments arranged by size: 588 372 122 77 19 11 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 289 358 427 496 565 634 703 778 Size: 289 69 69 69 69 69 69 75 Cuts at: 778 1011 1059 1197 Size: 233 48 138 Fragments arranged by size: 289 233 138 75 69 69 69 69 69 69 48 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 997 1029 1034 1066 1197 Size: 997 32 5 32 131 Fragments arranged by size: 997 131 32 32 5 Tth111I GACn'n_nGTC Cuts at: 0 874 1197 Size: 874 323 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 106 1197 Size: 106 1091 Enzymes that do cut: AcuI AlwNI ApaI ApoI BanI BanII BbeI BbsI BbvCI BglII Bme1580I Bpu10I BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BsrDI BstYI BtgI BtsI ClaI EaeI EcoICRI Eco57MI EcoNI EcoO109I HaeII HincII KasI MmeI MscI MspA1I NarI NcoI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SfcI SfoI StyI TaqII TstI Tth111I XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglI BlpI BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaBI BsaWI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EagI EarI EciI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI MluI MslI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI XbaI XhoI XmaI XmnI ZraI