################################## # NO CUT ENZYMES ( 175 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HphI KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TthHB8I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AcsI 561 GAATTC [GA]^AATT[CT] AlwI 298 GGATC GGATC(4/5) ApoI 561 GAATTC [GA]^AATT[CT] Asp700I 211 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 232 GTGCTC G[AT]GC[AT]^C AspI 84 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BcnI 22 CCGGG CC^[GC]GG BfaI 229 CTAG C^TAG BsaI 131 GGTCTC GGTCTC(1/5) BsiHKAI 232 GTGCTC G[AT]GC[AT]^C DpnII 299 GATC ^GATC EcoRI 561 GAATTC G^AATTC FauI 310 GCGGG CCCGC(4/6) HapII 22 CCGG C^CGG HgaI 225 GCGTC GACGC(5/10) HincII 600 GTTAAC GT[CT]^[GA]AC HindII 600 GTTAAC GT[CT]^[GA]AC HpaI 600 GTTAAC GTT^AAC HpaII 22 CCGG C^CGG MaeI 229 CTAG C^TAG MaeII 38 ACGT A^CGT MaeIII 682 GTGAC ^GT[ACGT]AC MboI 299 GATC ^GATC MboII 278 TCTTC GAAGA(8/7) MspA1I 78 CCGCTG C[AC]G^C[GT]G MspI 22 CCGG C^CGG NciI 22 CCGGG CC^[GC]GG NcoI 616 CCATGG C^CATGG NdeII 299 GATC ^GATC PflFI 84 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 13 GACTC GAGTC(4/5) Sau3AI 299 GATC ^GATC SexAI 85 ACCTGGT A^CC[AT]GGT Tsp45I 682 GTGAC ^GT[GC]AC Tth111I 84 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 678 CCATGTGACCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 211 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BbvI 373 GCTGC GCAGC(8/12) 376 GCTGC BsmAI 132 GTCTC GTCTC(1/5) 705 GTCTC BspHI 357 TCATGA T^CATGA 893 TCATGA BsrI 743 ACTGG ACTGG(1/-1) 555 CCAGT BstNI 9 CCTGG CC^[AT]GG 86 CCTGG DsaI 47 CCACGG C^C[GA][CT]GG 616 CCATGG EcoRII 9 CCTGG ^CC[AT]GG 86 CCTGG EcoT14I 136 CCTTGG C^C[AT][AT]GG 616 CCATGG Fnu4HI 373 GCTGC GC^[ACGT]GC 376 GCTGC ItaI 373 GCTGC GC^[ACGT]GC 376 GCTGC MvaI 9 CCTGG CC^[AT]GG 86 CCTGG PflMI 330 CCAGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 826 CCAAAAGCTGG RcaI 357 TCATGA T^CATGA 893 TCATGA StyI 136 CCTTGG C^C[AT][AT]GG 616 CCATGG TfiI 621 GATTC G^A[AT]TC 669 GATTC TseI 373 GCTGC G^C[AT]GC 376 GCTGC Van91I 330 CCAGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 826 CCAAAAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AciI AfaI AluI AvaII BanII BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I Cfr13I DdeI FokI HaeIII HinfI MnlI MseI MwoI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI Tru9I Tsp509I TspRI