################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BamHI BciVI BclI BfrI BglII BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI Eam1105I EarI Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KpnI Ksp632I MamI MfeI MluI MroI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SfuI SnaBI SpeI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AgeI 824 ACCGGT A^CCGGT AocI 1123 CCTCAGG CC^T[ACGT]AGG Asp700I 381 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 1066 CCCGGG C^[CT]CG[GA]G AvrII 2325 CCTAGG C^CTAGG BalI 2246 TGGCCA TGG^CCA BbeI 1591 GGCGCC GGCGC^C BbrPI 543 CACGTG CAC^GTG BbsI 1773 GAAGAC GAAGAC(2/6) BcgI 797 GCATCACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 2325 CCTAGG C^CTAGG BlpI 838 GCTGAGC GC^T[ACGT]AGC Bpu1102I 838 GCTGAGC GC^T[ACGT]AGC BpuAI 1773 GAAGAC GAAGAC(2/6) BsaAI 543 CACGTG [CT]AC^GT[GA] BsaI 1428 GGTCTC GGTCTC(1/5) BsaWI 824 ACCGGT [AT]^CCGG[AT] BsmBI 1470 CGTCTC CGTCTC(1/5) BsmI 1047 GAATGC GAATGC(1/-1) BsoBI 1066 CCCGGG C^[CT]CG[GA]G BstYI 812 AGATCC [GA]^GATC[CT] Bsu36I 1123 CCTCAGG CC^T[ACGT]AGG CelII 838 GCTGAGC GC^T[ACGT]AGC DraIII 543 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 1123 CCTCAGG CC^T[ACGT]AGG KasI 1591 GGCGCC G^GCGCC MflI 812 AGATCC [GA]^GATC[CT] MluNI 2246 TGGCCA TGG^CCA MscI 2246 TGGCCA TGG^CCA NarI 1591 GGCGCC GG^CGCC PflMI 270 CCAGGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 824 ACCGGT A^CCGGT PmaCI 543 CACGTG CAC^GTG PmlI 543 CACGTG CAC^GTG PshAI 1516 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 413 AGTACT AGT^ACT SexAI 269 ACCAGGT A^CC[AT]GGT SfiI 2247 GGCCAGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1591 GGCGCC GGC^GCC SgrAI 823 CACCGGTG C[GA]^CCGG[CT]G SmaI 1066 CCCGGG CCC^GGG SphI 770 GCATGC GCATG^C TfiI 190 GATTC G^A[AT]TC Van91I 270 CCAGGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1787 CCAAAGGGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 812 AGATCC [GA]^GATC[CT] XmaCI 1066 CCCGGG C^CCGGG XmaI 1066 CCCGGG C^CCGGG XmnI 381 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 310 GAATTT [GA]^AATT[CT] 513 GAATTT AcyI 1591 GGCGCC G[GA]^CG[CT]C 1719 GGCGTC AfaI 414 GTAC GT^AC 642 GTAC AlwI 762 GATCC GGATC(4/5) 813 GATCC ApoI 310 GAATTT [GA]^AATT[CT] 513 GAATTT BbvCI 2165 CCTCAGC CCTCAGC(-5/-2) 1767 GCTGAGG BsaHI 1591 GGCGCC G[GA]^CG[CT]C 1719 GGCGTC BsiEI 404 CGGCCG CG[GA][CT]^CG 2000 CGGCCG BstAPI 1489 GCAAGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1971 GCAGAGGCTGC DrdI 963 GACGGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2145 GACCGGCGCGTC EagI 404 CGGCCG C^GGCCG 2000 CGGCCG EclXI 404 CGGCCG C^GGCCG 2000 CGGCCG Eco52I 404 CGGCCG C^GGCCG 2000 CGGCCG EcoT14I 1184 CCTTGG C^C[AT][AT]GG 2325 CCTAGG HaeII 921 GGCGCT [GA]GCGC^[CT] 1591 GGCGCC Hin1I 1591 GGCGCC G[GA]^CG[CT]C 1719 GGCGTC HindIII 235 AAGCTT A^AGCTT 262 AAGCTT NaeI 1924 GCCGGC GCC^GGC 2034 GCCGGC NgoMIV 1924 GCCGGC G^CCGGC 2034 GCCGGC NspI 290 ACATGC [GA]CATG^[CT] 770 GCATGC RsaI 414 GTAC GT^AC 642 GTAC StuI 1751 AGGCCT AGG^CCT 1905 AGGCCT StyI 1184 CCTTGG C^C[AT][AT]GG 2325 CCTAGG ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AccII AciI AluI AlwNI AspHI AvaII BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI KspI MaeI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I