(Circular) (Six-base) MAPSORT of: pFN21AE3596.seq Check: 46 from: 1 to: 7054 pFN21AE3596 7054 bp With 182 enzymes: SgfI * March 28, 2012 12:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4206 4206 Size: 7054 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4864 278 Size: 53 83 186 936 3328 2468 Fragments arranged by size: 3328 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4397 1937 Size: 2460 4594 Acc65I G'GTAC_C Cuts at: 4375 4375 Size: 7054 AclI AA'CG_TT Cuts at: 1563 5181 5554 1563 Size: 3618 373 3063 Fragments arranged by size: 3618 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3773 5117 6501 6769 1632 Size: 2141 1344 1384 268 1917 Fragments arranged by size: 2141 1917 1384 1344 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6857 AflIII A'CryG_T Cuts at: 1203 5953 6930 1203 Size: 4750 977 1327 Fragments arranged by size: 4750 1327 977 AgeI A'CCGG_T Cuts at: 2829 5885 2829 Size: 3056 3998 AhdI GACnn_n'nnGTC Cuts at: 5783 5783 Size: 7054 AleI CACnn'nnGTG Cuts at: 1166 2367 3653 1166 Size: 1201 1286 4567 Fragments arranged by size: 4567 1286 1201 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4036 1184 Size: 2852 4202 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3672 3704 3672 Size: 32 7022 AlwNI CAG_nnn'CTG Cuts at: 2929 2947 3589 3772 3982 4186 4797 6369 2929 Size: 18 642 183 210 204 611 1572 3614 Fragments arranged by size: 3614 1572 642 611 210 204 183 18 ApaLI G'TGCA_C Cuts at: 5111 6267 5111 Size: 1156 5898 ApoI r'AATT_y Cuts at: 1493 2315 2518 4363 4589 4741 6850 1493 Size: 822 203 1845 226 152 2109 1697 Fragments arranged by size: 2109 1845 1697 822 226 203 152 AseI AT'TA_AT Cuts at: 160 1032 5608 160 Size: 872 4576 1606 Fragments arranged by size: 4576 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7054 AvaI C'yCGr_G Cuts at: 1870 1943 3071 4379 1870 Size: 73 1128 1308 4545 Fragments arranged by size: 4545 1308 1128 73 AvrII C'CTAG_G Cuts at: 4330 4330 Size: 7054 BamHI G'GATC_C Cuts at: 4384 4751 4384 Size: 367 6687 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2361 3124 3569 3596 4208 Size: 325 217 627 574 763 445 27 612 Cuts at: 4208 4375 5830 618 Size: 167 1455 1842 Fragments arranged by size: 1842 1455 763 627 612 574 445 325 217 167 27 BanII G_rGCy'C Cuts at: 1391 2564 2850 3104 3231 3239 3564 3884 3908 Size: 1173 286 254 127 8 325 320 24 Cuts at: 3908 4122 4278 4373 1391 Size: 214 156 95 4072 Fragments arranged by size: 4072 1173 325 320 286 254 214 156 127 95 24 8 BbeI G_GCGC'C Cuts at: 3600 3600 Size: 7054 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3785 928 Size: 971 1886 4197 Fragments arranged by size: 4197 1886 971 BbvCI CC'TCA_GC Cuts at: 3773 4171 3773 Size: 398 6656 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2791 2825 5245 5279 1903 Size: 34 854 34 2420 34 3678 Fragments arranged by size: 3678 2420 854 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4946 6156 4946 Size: 1210 5844 BclI T'GATC_A Cuts at: 1548 1548 Size: 7054 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3082 3860 3918 Size: 107 122 71 631 605 1410 778 58 Cuts at: 3918 3942 4175 4259 4282 5665 136 Size: 24 233 84 23 1383 1525 Fragments arranged by size: 1525 1410 1383 778 631 605 233 122 107 84 71 58 24 23 BglII A'GATC_T Cuts at: 7049 7049 Size: 7054 BlpI GC'TnA_GC Cuts at: 2844 4471 2844 Size: 1627 5427 Bme1580I G_kGCm'C Cuts at: 1790 2364 3176 4239 5115 6271 1790 Size: 574 812 1063 876 1156 2573 Fragments arranged by size: 2573 1156 1063 876 812 574 BmgBI CAC'GTC Cuts at: 1318 4113 1318 Size: 2795 4259 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3052 3647 4785 Size: 416 53 83 399 24 1635 595 1138 Cuts at: 4785 5743 442 Size: 958 1753 Fragments arranged by size: 1753 1635 1138 958 595 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7054 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2647 2735 3191 3215 3967 3971 3977 5714 Size: 1408 88 456 24 752 4 6 1737 Cuts at: 5714 1239 Size: 2579 Fragments arranged by size: 2579 1737 1408 752 456 88 24 6 4 Bpu10I CC'TnA_GC Cuts at: 3666 3773 3878 4171 3666 Size: 107 105 293 6549 Fragments arranged by size: 6549 293 107 105 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2438 2733 3023 3050 3729 5179 6044 6342 2438 Size: 295 290 27 679 1450 865 298 3150 Fragments arranged by size: 3150 1450 865 679 298 295 290 27 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3439 5717 882 Size: 617 1940 2278 2219 Fragments arranged by size: 2278 2219 1940 617 BsaAI yAC'GTr Cuts at: 493 1204 2550 4845 493 Size: 711 1346 2295 2702 Fragments arranged by size: 2702 2295 1346 711 BsaBI GATnn'nnATC Cuts at: 7048 7048 Size: 7054 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3597 3725 4861 5243 Size: 53 83 186 936 2064 128 1136 382 Cuts at: 5243 5878 275 Size: 635 1451 Fragments arranged by size: 2064 1451 1136 936 635 382 186 128 83 53 BsaWI w'CCGG_w Cuts at: 2829 5487 5885 6159 6306 6717 2829 Size: 2658 398 274 147 411 3166 Fragments arranged by size: 3166 2658 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3672 3702 1178 Size: 30 165 30 2269 30 4530 Fragments arranged by size: 4530 2269 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3014 3356 3383 3386 3696 3974 4097 1186 Size: 1828 342 27 3 310 278 123 4143 Fragments arranged by size: 4143 1828 342 310 278 123 27 3 BseYI C'CCAG_C Cuts at: 2613 2929 3398 3592 4035 4097 6257 6753 2613 Size: 316 469 194 443 62 2160 496 2914 Fragments arranged by size: 2914 2160 496 469 443 316 194 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3215 3882 3899 3996 4184 4370 1163 Size: 573 1479 667 17 97 188 186 3847 Fragments arranged by size: 3847 1479 667 573 188 186 97 17 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2412 4008 4504 5265 6293 1540 Size: 275 186 411 1596 496 761 1028 2301 Fragments arranged by size: 2301 1596 1028 761 496 411 275 186 BsiHKAI G_wGCw'C Cuts at: 3104 3231 3239 5115 5200 6271 3104 Size: 127 8 1876 85 1071 3887 Fragments arranged by size: 3887 1876 1071 127 85 8 BsmI GAATG_Cn' Cuts at: 3058 4565 4658 3058 Size: 1507 93 5454 Fragments arranged by size: 5454 1507 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3481 3481 Size: 7054 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2364 2564 2850 3104 3176 3231 3239 Size: 399 574 200 286 254 72 55 8 Cuts at: 3239 3564 3884 3908 4122 4239 4278 4373 5115 Size: 325 320 24 214 117 39 95 742 Cuts at: 5115 5200 6271 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 574 399 325 320 286 254 214 200 117 95 85 72 55 39 24 8 BspEI T'CCGG_A Cuts at: 6717 6717 Size: 7054 BspHI T'CATG_A Cuts at: 4943 6673 4943 Size: 1730 5324 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2149 2697 3169 4206 4342 4409 844 Size: 1305 548 472 1037 136 67 3489 Fragments arranged by size: 3489 1305 1037 548 472 136 67 BsrBI CCG'CTC Cuts at: 1394 1823 2095 3034 4093 4941 1394 Size: 429 272 939 1059 848 3507 Fragments arranged by size: 3507 1059 939 848 429 272 BsrDI GCAATG_nn' Cuts at: 65 1248 5549 5723 65 Size: 1183 4301 174 1396 Fragments arranged by size: 4301 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2829 2958 3929 4039 4151 4211 5698 Size: 110 1019 129 971 110 112 60 1487 Cuts at: 5698 5885 1700 Size: 187 2869 Fragments arranged by size: 2869 1487 1019 971 187 129 112 110 110 60 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7054 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7054 BssSI C'ACGA_G Cuts at: 5114 6126 5114 Size: 1012 6042 BstAPI GCAn_nnn'nTGC Cuts at: 794 3500 3982 794 Size: 2706 482 3866 Fragments arranged by size: 3866 2706 482 BstBI TT'CG_AA Cuts at: 5858 5858 Size: 7054 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7054 BstYI r'GATC_y Cuts at: 1972 2817 4384 4751 5136 5153 6605 6691 6703 Size: 845 1567 367 385 17 1452 86 12 Cuts at: 6703 7049 1972 Size: 346 1977 Fragments arranged by size: 1977 1567 1452 845 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3129 3129 Size: 7054 BtgI C'CryG_G Cuts at: 513 1065 2004 2037 2412 3089 3489 3555 513 Size: 552 939 33 375 677 400 66 4012 Fragments arranged by size: 4012 939 677 552 400 375 66 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2794 487 Size: 683 56 1568 4747 Fragments arranged by size: 4747 1568 683 56 BtsI GCAGTG_nn' Cuts at: 2978 3251 4570 5364 5384 2978 Size: 273 1319 794 20 4648 Fragments arranged by size: 4648 1319 794 273 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7054 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7019 DraI TTT'AAA Cuts at: 4358 4358 Size: 7054 DraIII CAC_nnn'GTG Cuts at: 2553 2553 Size: 7054 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2974 4156 6061 809 Size: 507 1658 1182 1905 1802 Fragments arranged by size: 1905 1802 1658 1182 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2409 3426 3576 Size: 54 1425 50 275 18 579 1017 150 Cuts at: 3576 4005 4251 4501 5390 7019 8 Size: 429 246 250 889 1629 43 Fragments arranged by size: 1629 1425 1017 889 579 429 275 250 246 150 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2409 4005 4501 1537 Size: 275 597 1596 496 4090 Fragments arranged by size: 4090 1596 597 496 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4984 1353 Size: 3631 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2759 3634 5641 6015 6161 387 Size: 1064 138 1170 875 2007 374 146 1280 Fragments arranged by size: 2007 1280 1170 1064 875 374 146 138 EcoICRI GAG'CTC Cuts at: 3102 3229 3237 3102 Size: 127 8 6919 Fragments arranged by size: 6919 127 8 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2647 2735 3191 3215 3773 3967 3971 Size: 393 1015 88 456 24 558 194 4 Cuts at: 3971 3977 5117 5714 6501 6769 1239 Size: 6 1140 597 787 268 1524 Fragments arranged by size: 1524 1140 1015 787 597 558 456 393 268 194 88 24 6 4 EcoNI CCTnn'n_nnAGG Cuts at: 3132 3368 3428 3956 4349 3132 Size: 236 60 528 393 5837 Fragments arranged by size: 5837 528 393 236 60 EcoO109I rG'GnC_Cy Cuts at: 2449 2609 2861 3847 4030 4312 2449 Size: 160 252 986 183 282 5191 Fragments arranged by size: 5191 986 282 252 183 160 EcoRI G'AATT_C Cuts at: 4363 4741 4363 Size: 378 6676 FspI TGC'GCA Cuts at: 5560 5560 Size: 7054 HaeII r_GCGC'y Cuts at: 1678 2930 3600 6201 1678 Size: 1252 670 2601 2531 Fragments arranged by size: 2601 2531 1252 670 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2528 2560 5695 5727 5769 Size: 32 135 32 956 32 3135 32 42 Cuts at: 5769 5801 1373 Size: 32 2626 Fragments arranged by size: 3135 2626 956 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4398 4644 669 Size: 988 281 2460 246 3079 Fragments arranged by size: 3079 2460 988 281 246 HindIII A'AGCT_T Cuts at: 748 2240 2267 748 Size: 1492 27 5535 Fragments arranged by size: 5535 1492 27 HpaI GTT'AAC Cuts at: 4644 4644 Size: 7054 KasI G'GCGC_C Cuts at: 3596 3596 Size: 7054 KpnI G_GTAC'C Cuts at: 4379 4379 Size: 7054 MfeI C'AATT_G Cuts at: 4653 4653 Size: 7054 MluI A'CGCG_T Cuts at: 6930 6930 Size: 7054 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1998 2379 2925 3181 3708 6168 6352 1305 Size: 693 381 546 256 527 2460 184 2007 Fragments arranged by size: 2460 2007 693 546 527 381 256 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4253 10 Size: 54 1425 343 2421 2811 Fragments arranged by size: 2811 2421 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2367 2780 3653 5012 5371 5530 518 Size: 648 1201 413 873 1359 359 159 2042 Fragments arranged by size: 2042 1359 1201 873 648 413 359 159 MspA1I CmG'CkG Cuts at: 1606 2039 2233 2617 2707 3091 3276 3282 3557 Size: 433 194 384 90 384 185 6 275 Cuts at: 3557 3772 4010 4126 4175 4232 4470 5147 6295 Size: 215 238 116 49 57 238 677 1148 Cuts at: 6295 6540 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 433 384 384 275 245 238 238 215 194 185 116 90 57 49 6 NaeI GCC'GGC Cuts at: 3931 4041 3931 Size: 110 6944 NarI GG'CG_CC Cuts at: 3597 3597 Size: 7054 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5563 Fragments arranged by size: 5563 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7054 NgoMIV G'CCGG_C Cuts at: 3929 4039 3929 Size: 110 6944 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7054 NotI GC'GGCC_GC Cuts at: 4501 4501 Size: 7054 NspI r_CATG'y Cuts at: 1132 1229 2299 2779 4412 5957 1132 Size: 97 1070 480 1633 1545 2229 Fragments arranged by size: 2229 1633 1545 1070 480 97 PasI CC'CwG_GG Cuts at: 1348 2101 2947 4079 4279 1348 Size: 753 846 1132 200 4123 Fragments arranged by size: 4123 1132 846 753 200 PciI A'CATG_T Cuts at: 5953 5953 Size: 7054 PflMI CCAn_nnn'nTGG Cuts at: 1108 2281 1108 Size: 1173 5881 PfoI T'CCnGG_A Cuts at: 3708 4185 3708 Size: 477 6577 PmeI GTTT'AAAC Cuts at: 4358 4358 Size: 7054 PmlI CAC'GTG Cuts at: 2550 2550 Size: 7054 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3672 3704 5123 5155 6636 6668 3672 Size: 32 1419 32 1481 32 4058 Fragments arranged by size: 4058 1481 1419 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2449 2861 3847 4312 2449 Size: 412 986 465 5191 Fragments arranged by size: 5191 986 465 412 PshAI GACnn'nnGTC Cuts at: 1613 3525 1613 Size: 1912 5142 PsiI TTA'TAA Cuts at: 4624 4624 Size: 7054 PstI C_TGCA'G Cuts at: 830 3246 3281 3505 3663 4149 4293 4406 830 Size: 2416 35 224 158 486 144 113 3478 Fragments arranged by size: 3478 2416 486 224 158 144 113 35 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7054 PvuII CAG'CTG Cuts at: 2233 2617 3276 3772 2233 Size: 384 659 496 5515 Fragments arranged by size: 5515 659 496 384 SacI G_AGCT'C Cuts at: 3104 3231 3239 3104 Size: 127 8 6919 Fragments arranged by size: 6919 127 8 SacII CC_GC'GG Cuts at: 2040 3092 3558 2040 Size: 1052 466 5536 Fragments arranged by size: 5536 1052 466 SalI G'TCGA_C Cuts at: 1936 4396 1936 Size: 2460 4594 SanDI GG'GwC_CC Cuts at: 2861 2861 Size: 7054 SbfI CC_TGCA'GG Cuts at: 4406 4406 Size: 7054 ScaI AGT'ACT Cuts at: 2420 5302 7014 2420 Size: 2882 1712 2460 Fragments arranged by size: 2882 2460 1712 SexAI A'CCwGG_T Cuts at: 2274 2274 Size: 7054 SfcI C'TryA_G Cuts at: 826 1045 3242 3277 3501 3659 4145 4289 4402 Size: 219 2197 35 224 158 486 144 113 Cuts at: 4402 5537 6218 6409 826 Size: 1135 681 191 1471 Fragments arranged by size: 2197 1471 1135 681 486 224 219 191 158 144 113 35 SfiI GGCCn_nnn'nGGCC Cuts at: 4259 4259 Size: 7054 SfoI GGC'GCC Cuts at: 3598 3598 Size: 7054 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7054 SgrAI Cr'CCGG_yG Cuts at: 1700 2829 1700 Size: 1129 5925 SmaI CCC'GGG Cuts at: 1872 3073 4381 1872 Size: 1201 1308 4545 Fragments arranged by size: 4545 1308 1201 SmlI C'TyrA_G Cuts at: 820 1017 1943 2453 2748 3029 3038 3744 5158 Size: 197 926 510 295 281 9 706 1414 Cuts at: 5158 6059 6321 820 Size: 901 262 1553 Fragments arranged by size: 1553 1414 926 901 706 510 295 281 262 197 9 SnaBI TAC'GTA Cuts at: 493 4845 493 Size: 4352 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7054 SphI G_CATG'C Cuts at: 1132 2779 4412 1132 Size: 1647 1633 3774 Fragments arranged by size: 3774 1647 1633 SspI AAT'ATT Cuts at: 5 52 4978 5 Size: 47 4926 2081 Fragments arranged by size: 4926 2081 47 StuI AGG'CCT Cuts at: 1517 3758 3912 1517 Size: 2241 154 4659 Fragments arranged by size: 4659 2241 154 StyI C'CwwG_G Cuts at: 513 1065 2004 3189 4330 4493 513 Size: 552 939 1185 1141 163 3074 Fragments arranged by size: 3074 1185 1141 939 552 163 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2474 5081 5098 5251 5436 5881 325 Size: 1255 894 2607 17 153 185 445 1498 Fragments arranged by size: 2607 1498 1255 894 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2418 5300 7012 Size: 274 80 33 51 1443 441 2882 1712 Cuts at: 7012 96 Size: 138 Fragments arranged by size: 2882 1712 1443 441 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3481 3513 7014 7046 3481 Size: 32 3501 32 3489 Fragments arranged by size: 3501 3489 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7054 XbaI T'CTAG_A Cuts at: 4390 4390 Size: 7054 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3799 3799 Size: 7054 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7054 XmaI C'CCGG_G Cuts at: 1870 3071 4379 1870 Size: 1201 1308 4545 Fragments arranged by size: 4545 1308 1201 XmnI GAAnn'nnTTC Cuts at: 2390 5183 2390 Size: 2793 4261 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4862 276 Size: 53 83 186 936 3328 2468 Fragments arranged by size: 3328 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BfrBI BplI BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SapI SrfI SwaI