(Linear) (Six-base) MAPSORT of: KIEE3596.seq Check: 3359 from: 1 to: 2349 KIEE3596.orf With 182 enzymes: SgfI * March 22, 2012 16:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2201 2349 Size: 2201 148 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1768 2349 Size: 1768 581 AgeI A'CCGG_T Cuts at: 0 824 2349 Size: 824 1525 AleI CACnn'nnGTG Cuts at: 0 362 1648 2349 Size: 362 1286 701 Fragments arranged by size: 1286 701 362 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2031 2349 Size: 2031 318 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1667 1699 2349 Size: 1667 32 650 Fragments arranged by size: 1667 650 32 AlwNI CAG_nnn'CTG Cuts at: 0 924 942 1584 1767 1977 2181 2349 Size: 924 18 642 183 210 204 168 Fragments arranged by size: 924 642 210 204 183 168 18 ApoI r'AATT_y Cuts at: 0 310 513 2349 Size: 310 203 1836 Fragments arranged by size: 1836 310 203 AvaI C'yCGr_G Cuts at: 0 1066 2349 Size: 1066 1283 AvrII C'CTAG_G Cuts at: 0 2325 2349 Size: 2325 24 BanI G'GyrC_C Cuts at: 0 356 1119 1564 1591 2203 2349 Size: 356 763 445 27 612 146 Fragments arranged by size: 763 612 445 356 146 27 BanII G_rGCy'C Cuts at: 0 559 845 1099 1226 1234 1559 1879 1903 Size: 559 286 254 127 8 325 320 24 Cuts at: 1903 2117 2273 2349 Size: 214 156 76 Fragments arranged by size: 559 325 320 286 254 214 156 127 76 24 8 BbeI G_GCGC'C Cuts at: 0 1595 2349 Size: 1595 754 BbsI GAAGACnn'nnnn_ Cuts at: 0 1780 2349 Size: 1780 569 BbvCI CC'TCA_GC Cuts at: 0 1768 2166 2349 Size: 1768 398 183 Fragments arranged by size: 1768 398 183 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 786 820 2349 Size: 786 34 1529 Fragments arranged by size: 1529 786 34 BglI GCCn_nnn'nGGC Cuts at: 0 1077 1855 1913 1937 2170 2254 2277 2349 Size: 1077 778 58 24 233 84 23 72 Fragments arranged by size: 1077 778 233 84 72 58 24 23 BlpI GC'TnA_GC Cuts at: 0 839 2349 Size: 839 1510 Bme1580I G_kGCm'C Cuts at: 0 359 1171 2234 2349 Size: 359 812 1063 115 Fragments arranged by size: 1063 812 359 115 BmgBI CAC'GTC Cuts at: 0 2108 2349 Size: 2108 241 BmrI ACTGGGnnnn_n' Cuts at: 0 1047 1642 2349 Size: 1047 595 707 Fragments arranged by size: 1047 707 595 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 642 730 1186 1210 1962 1966 1972 2349 Size: 642 88 456 24 752 4 6 377 Fragments arranged by size: 752 642 456 377 88 24 6 4 Bpu10I CC'TnA_GC Cuts at: 0 1661 1768 1873 2166 2349 Size: 1661 107 105 293 183 Fragments arranged by size: 1661 293 183 107 105 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 433 728 1018 1045 1724 2349 Size: 433 295 290 27 679 625 Fragments arranged by size: 679 625 433 295 290 27 BsaI GGTCTCn'nnnn_ Cuts at: 0 1434 2349 Size: 1434 915 BsaAI yAC'GTr Cuts at: 0 545 2349 Size: 545 1804 BsaHI Gr'CG_yC Cuts at: 0 1592 1720 2349 Size: 1592 128 629 Fragments arranged by size: 1592 629 128 BsaWI w'CCGG_w Cuts at: 0 824 2349 Size: 824 1525 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1667 1697 2349 Size: 1667 30 652 Fragments arranged by size: 1667 652 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1009 1351 1378 1381 1691 1969 2092 2349 Size: 1009 342 27 3 310 278 123 257 Fragments arranged by size: 1009 342 310 278 257 123 27 3 BseYI C'CCAG_C Cuts at: 0 608 924 1393 1587 2030 2092 2349 Size: 608 316 469 194 443 62 257 Fragments arranged by size: 608 469 443 316 257 194 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1210 1877 1894 1991 2179 2349 Size: 1210 667 17 97 188 170 Fragments arranged by size: 1210 667 188 170 97 17 BsiEI CG_ry'CG Cuts at: 0 407 2003 2349 Size: 407 1596 346 Fragments arranged by size: 1596 407 346 BsiHKAI G_wGCw'C Cuts at: 0 1099 1226 1234 2349 Size: 1099 127 8 1115 Fragments arranged by size: 1115 1099 127 8 BsmI GAATG_Cn' Cuts at: 0 1053 2349 Size: 1053 1296 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1476 2349 Size: 1476 873 Bsp1286I G_dGCh'C Cuts at: 0 359 559 845 1099 1171 1226 1234 1559 Size: 359 200 286 254 72 55 8 325 Cuts at: 1559 1879 1903 2117 2234 2273 2349 Size: 320 24 214 117 39 76 Fragments arranged by size: 359 325 320 286 254 214 200 117 76 72 55 39 24 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 144 692 1164 2201 2349 Size: 144 548 472 1037 148 Fragments arranged by size: 1037 548 472 148 144 BsrBI CCG'CTC Cuts at: 0 90 1029 2088 2349 Size: 90 939 1059 261 Fragments arranged by size: 1059 939 261 90 BsrFI r'CCGG_y Cuts at: 0 824 953 1924 2034 2146 2206 2349 Size: 824 129 971 110 112 60 143 Fragments arranged by size: 971 824 143 129 112 110 60 BstAPI GCAn_nnn'nTGC Cuts at: 0 1495 1977 2349 Size: 1495 482 372 Fragments arranged by size: 1495 482 372 BstYI r'GATC_y Cuts at: 0 812 2349 Size: 812 1537 Bsu36I CC'TnA_GG Cuts at: 0 1124 2349 Size: 1124 1225 BtgI C'CryG_G Cuts at: 0 32 407 1084 1484 1550 2349 Size: 32 375 677 400 66 799 Fragments arranged by size: 799 677 400 375 66 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 789 2349 Size: 789 1560 BtsI GCAGTG_nn' Cuts at: 0 973 1246 2349 Size: 973 273 1103 Fragments arranged by size: 1103 973 273 DraIII CAC_nnn'GTG Cuts at: 0 548 2349 Size: 548 1801 DrdI GACnn_nn'nnGTC Cuts at: 0 969 2151 2349 Size: 969 1182 198 Fragments arranged by size: 1182 969 198 EaeI y'GGCC_r Cuts at: 0 404 1421 1571 2000 2246 2349 Size: 404 1017 150 429 246 103 Fragments arranged by size: 1017 429 404 246 150 103 EagI C'GGCC_G Cuts at: 0 404 2000 2349 Size: 404 1596 349 Fragments arranged by size: 1596 404 349 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 754 1629 2349 Size: 754 875 720 Fragments arranged by size: 875 754 720 EcoICRI GAG'CTC Cuts at: 0 1097 1224 1232 2349 Size: 1097 127 8 1117 Fragments arranged by size: 1117 1097 127 8 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 642 730 1186 1210 1768 1962 1966 1972 Size: 642 88 456 24 558 194 4 6 Cuts at: 1972 2349 Size: 377 Fragments arranged by size: 642 558 456 377 194 88 24 6 4 EcoNI CCTnn'n_nnAGG Cuts at: 0 1127 1363 1423 1951 2349 Size: 1127 236 60 528 398 Fragments arranged by size: 1127 528 398 236 60 EcoO109I rG'GnC_Cy Cuts at: 0 444 604 856 1842 2025 2307 2349 Size: 444 160 252 986 183 282 42 Fragments arranged by size: 986 444 282 252 183 160 42 HaeII r_GCGC'y Cuts at: 0 925 1595 2349 Size: 925 670 754 Fragments arranged by size: 925 754 670 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 523 555 2349 Size: 523 32 1794 Fragments arranged by size: 1794 523 32 HindIII A'AGCT_T Cuts at: 0 235 262 2349 Size: 235 27 2087 Fragments arranged by size: 2087 235 27 KasI G'GCGC_C Cuts at: 0 1591 2349 Size: 1591 758 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 374 920 1176 1703 2349 Size: 374 546 256 527 646 Fragments arranged by size: 646 546 527 374 256 MscI TGG'CCA Cuts at: 0 2248 2349 Size: 2248 101 MslI CAynn'nnrTG Cuts at: 0 362 775 1648 2349 Size: 362 413 873 701 Fragments arranged by size: 873 701 413 362 MspA1I CmG'CkG Cuts at: 0 34 228 612 702 1086 1271 1277 1552 Size: 34 194 384 90 384 185 6 275 Cuts at: 1552 1767 2005 2121 2170 2227 2349 Size: 215 238 116 49 57 122 Fragments arranged by size: 384 384 275 238 215 194 185 122 116 90 57 49 34 6 NaeI GCC'GGC Cuts at: 0 1926 2036 2349 Size: 1926 110 313 Fragments arranged by size: 1926 313 110 NarI GG'CG_CC Cuts at: 0 1592 2349 Size: 1592 757 NgoMIV G'CCGG_C Cuts at: 0 1924 2034 2349 Size: 1924 110 315 Fragments arranged by size: 1924 315 110 NspI r_CATG'y Cuts at: 0 294 774 2349 Size: 294 480 1575 Fragments arranged by size: 1575 480 294 PasI CC'CwG_GG Cuts at: 0 96 942 2074 2274 2349 Size: 96 846 1132 200 75 Fragments arranged by size: 1132 846 200 96 75 PflMI CCAn_nnn'nTGG Cuts at: 0 276 2349 Size: 276 2073 PfoI T'CCnGG_A Cuts at: 0 1703 2180 2349 Size: 1703 477 169 Fragments arranged by size: 1703 477 169 PmlI CAC'GTG Cuts at: 0 545 2349 Size: 545 1804 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1667 1699 2349 Size: 1667 32 650 Fragments arranged by size: 1667 650 32 PpuMI rG'GwC_Cy Cuts at: 0 444 856 1842 2307 2349 Size: 444 412 986 465 42 Fragments arranged by size: 986 465 444 412 42 PshAI GACnn'nnGTC Cuts at: 0 1520 2349 Size: 1520 829 PstI C_TGCA'G Cuts at: 0 1241 1276 1500 1658 2144 2288 2349 Size: 1241 35 224 158 486 144 61 Fragments arranged by size: 1241 486 224 158 144 61 35 PvuII CAG'CTG Cuts at: 0 228 612 1271 1767 2349 Size: 228 384 659 496 582 Fragments arranged by size: 659 582 496 384 228 SacI G_AGCT'C Cuts at: 0 1099 1226 1234 2349 Size: 1099 127 8 1115 Fragments arranged by size: 1115 1099 127 8 SacII CC_GC'GG Cuts at: 0 35 1087 1553 2349 Size: 35 1052 466 796 Fragments arranged by size: 1052 796 466 35 SanDI GG'GwC_CC Cuts at: 0 856 2349 Size: 856 1493 ScaI AGT'ACT Cuts at: 0 415 2349 Size: 415 1934 SexAI A'CCwGG_T Cuts at: 0 269 2349 Size: 269 2080 SfcI C'TryA_G Cuts at: 0 1237 1272 1496 1654 2140 2284 2349 Size: 1237 35 224 158 486 144 65 Fragments arranged by size: 1237 486 224 158 144 65 35 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2254 2349 Size: 2254 95 SfoI GGC'GCC Cuts at: 0 1593 2349 Size: 1593 756 SgrAI Cr'CCGG_yG Cuts at: 0 824 2349 Size: 824 1525 SmaI CCC'GGG Cuts at: 0 1068 2349 Size: 1068 1281 SmlI C'TyrA_G Cuts at: 0 448 743 1024 1033 1739 2349 Size: 448 295 281 9 706 610 Fragments arranged by size: 706 610 448 295 281 9 SphI G_CATG'C Cuts at: 0 774 2349 Size: 774 1575 StuI AGG'CCT Cuts at: 0 1753 1907 2349 Size: 1753 154 442 Fragments arranged by size: 1753 442 154 StyI C'CwwG_G Cuts at: 0 1184 2325 2349 Size: 1184 1141 24 Fragments arranged by size: 1184 1141 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 469 2349 Size: 469 1880 TatI w'GTAC_w Cuts at: 0 413 2349 Size: 413 1936 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1476 1508 2349 Size: 1476 32 841 Fragments arranged by size: 1476 841 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1794 2349 Size: 1794 555 XmaI C'CCGG_G Cuts at: 0 1066 2349 Size: 1066 1283 XmnI GAAnn'nnTTC Cuts at: 0 385 2349 Size: 385 1964 Enzymes that do cut: AarI AcuI AgeI AleI AlfI AloI AlwNI ApoI AvaI AvrII BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BstAPI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PflMI PfoI PmlI PpiI PpuMI PshAI PstI PvuII SacI SacII SanDI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SphI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AhdI ApaI ApaLI AscI AseI AsiSI BaeI BamHI BciVI BclI BfrBI BglII BmtI BplI BsaBI BsiWI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I ClaI CspCI DraI EarI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KpnI MfeI MluI NcoI NdeI NheI NotI NruI NsiI PacI PciI PmeI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SapI SbfI SgfI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI ZraI