################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII AccII AclI AflII AgeI AhdI ApaI AscI AseI AsnI AspEI AspI AviII AvrII BbvCI BcgI BfrI BlnI BsiEI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI BstEII BstPI BstUI Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I EcoO65I EcoRV FseI FspI HgaI HpaI KspI MfeI MluI MunI MvnI NaeI NcoI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SfiI SgrAI SmaI SnaBI SphI Sse8387I SspBI SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### Acc65I 1895 GGTACC G^GTACC AccI 870 GTATAC GT^[AC][GT]AC AccIII 34 TCCGGA T^CCGGA AcyI 1926 GGCGCC G[GA]^CG[CT]C AflIII 1319 ACACGT A^C[GA][CT]GT Alw44I 3148 GTGCAC G^TGCAC AlwNI 2357 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 2821 CCTGAGG CC^T[ACGT]AGG Aor51HI 400 AGCGCT AGC^GCT ApaLI 3148 GTGCAC G^TGCAC Asp718I 1895 GGTACC G^GTACC AvaI 90 CTCGGG C^[CT]CG[GA]G BanII 365 GAGCTC G[GA]GC[CT]^C BbeI 1926 GGCGCC GGCGC^C BciVI 2479 GTATCC GTATCC(6/5) BcnI 645 CCCGG CC^[GC]GG BglI 1325 GCCTCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 958 GCTCAGC GC^T[ACGT]AGC Bpu1102I 958 GCTCAGC GC^T[ACGT]AGC BsaHI 1926 GGCGCC G[GA]^CG[CT]C BsaWI 34 TCCGGA [AT]^CCGG[AT] BseAI 34 TCCGGA T^CCGGA BsmFI 3103 GTCCC GGGAC(10/14) BsoBI 90 CTCGGG C^[CT]CG[GA]G BspEI 34 TCCGGA T^CCGGA BspHI 449 TCATGA T^CATGA Bst1107I 870 GTATAC GTA^TAC BstBI 1943 TTCGAA TT^CGAA BstZ17I 870 GTATAC GTA^TAC Bsu36I 2821 CCTGAGG CC^T[ACGT]AGG CelII 958 GCTCAGC GC^T[ACGT]AGC DraI 1831 TTTAAA TTT^AAA Eco47III 400 AGCGCT AGC^GCT Eco81I 2821 CCTGAGG CC^T[ACGT]AGG EcoNI 3270 CCTGTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2007 GAATTC G^AATTC FauI 3252 GCGGG CCCGC(4/6) Hin1I 1926 GGCGCC G[GA]^CG[CT]C KasI 1926 GGCGCC G^GCGCC KpnI 1895 GGTACC GGTAC^C MroI 34 TCCGGA T^CCGGA MslI 2781 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 961 CAGCTG C[AC]G^C[GT]G NarI 1926 GGCGCC GG^CGCC NciI 645 CCCGG CC^[GC]GG NspV 1943 TTCGAA TT^CGAA PflMI 2929 CCACCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 374 AGGACCC [GA]G^G[AT]CC[CT] PshAI 2186 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1600 CTGCAG CTGCA^G PvuII 961 CAGCTG CAG^CTG RcaI 449 TCATGA T^CATGA SacI 365 GAGCTC GAGCT^C ScaI 2581 AGTACT AGT^ACT SexAI 302 ACCTGGT A^CC[AT]GGT SfoI 1926 GGCGCC GGC^GCC SfuI 1943 TTCGAA TT^CGAA StuI 2196 AGGCCT AGG^CCT Van91I 2929 CCACCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2454 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AspHI 365 GAGCTC G[AT]GC[AT]^C 3148 GTGCAC AvaII 375 GGACC G^G[AT]CC 1979 GGACC BamHI 879 GGATCC G^GATCC 2827 GGATCC BbrPI 1320 CACGTG CAC^GTG 2611 CACGTG BbsI 799 GAAGAC GAAGAC(2/6) 2326 GAAGAC BmyI 365 GAGCTC G[AGT]GC[ACT]^C 3148 GTGCAC BpmI 2160 CTCCAG CTGGAG(16/14) 2486 CTCCAG BpuAI 799 GAAGAC GAAGAC(2/6) 2326 GAAGAC BsaAI 1320 CACGTG [CT]AC^GT[GA] 2611 CACGTG BsaI 1712 GAGACC GGTCTC(1/5) 2697 GGTCTC BsiHKAI 365 GAGCTC G[AT]GC[AT]^C 3148 GTGCAC BsmI 504 GAATGC GAATGC(1/-1) 933 GAATGC Bsp1286I 365 GAGCTC G[AGT]GC[ACT]^C 3148 GTGCAC BspMI 1416 ACCTGC ACCTGC(4/8) 720 GCAGGT BsrDI 3030 CATTGC GCAATG(2/0) 2135 GCAATG BstAPI 508 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2988 GCAAGGAGTGC BstXI 1298 CCAACATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1451 CCAAACTCCTGG CfoI 401 GCGC GCG^C 1927 GCGC DraII 94 GGGGCCT [GA]G^G[ACGT]CC[CT] 374 AGGACCC EcoO109I 94 GGGGCCT [GA]G^G[ACGT]CC[CT] 374 AGGACCC EcoT14I 775 CCTTGG C^C[AT][AT]GG 2897 CCAAGG EcoT22I 224 ATGCAT ATGCA^T 2788 ATGCAT HaeII 400 AGCGCT [GA]GCGC^[CT] 1926 GGCGCC HapII 35 CCGG C^CGG 646 CCGG HhaI 401 GCGC GCG^C 1927 GCGC HinP1I 401 GCGC G^CGC 1927 GCGC HincII 1134 GTCAAC GT[CT]^[GA]AC 2553 GTCAAC HindII 1134 GTCAAC GT[CT]^[GA]AC 2553 GTCAAC HpaII 35 CCGG C^CGG 646 CCGG MaeII 1321 ACGT A^CGT 2612 ACGT MspI 35 CCGG C^CGG 646 CCGG NdeI 491 CATATG CA^TATG 1444 CATATG NsiI 224 ATGCAT ATGCA^T 2788 ATGCAT NspI 737 ACATGC [GA]CATG^[CT] 2790 GCATGT PmaCI 1320 CACGTG CAC^GTG 2611 CACGTG PmlI 1320 CACGTG CAC^GTG 2611 CACGTG SmlI 1819 CTTGAG C^T[CT][GA]AG 2815 CTTGAG StyI 775 CCTTGG C^C[AT][AT]GG 2897 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I BalI BanI BbvI BclI BfaI BglII BsaBI BsaJI BseRI BsgI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EaeI EarI EcoRII FbaI Fnu4HI FokI HaeIII HindIII HinfI HphI ItaI Ksp632I MaeI MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SpeI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI