(Circular) (Six-base) MAPSORT of: pFN21AE4590.seq Check: 5105 from: 1 to: 7987 pFN21AE4590 7987 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5797 278 Size: 53 83 186 936 4261 2468 Fragments arranged by size: 4261 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2876 5330 1937 Size: 939 2454 4594 Fragments arranged by size: 4594 2454 939 Acc65I G'GTAC_C Cuts at: 3900 5308 3900 Size: 1408 6579 AclI AA'CG_TT Cuts at: 1563 6114 6487 1563 Size: 4551 373 3063 Fragments arranged by size: 4551 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2343 2396 2912 3015 3140 3188 4281 4554 Size: 711 53 516 103 125 48 1093 273 Cuts at: 4554 5210 5285 6050 7434 7702 1632 Size: 656 75 765 1384 268 1917 Fragments arranged by size: 1917 1384 1093 765 711 656 516 273 268 125 103 75 53 48 AfeI AGC'GCT Cuts at: 2407 2407 Size: 7987 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7790 AflIII A'CryG_T Cuts at: 1203 3324 6886 7863 1203 Size: 2121 3562 977 1327 Fragments arranged by size: 3562 2121 1327 977 AgeI A'CCGG_T Cuts at: 6818 6818 Size: 7987 AhdI GACnn_n'nnGTC Cuts at: 6716 6716 Size: 7987 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7987 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2355 4808 1184 Size: 1171 2453 4363 Fragments arranged by size: 4363 2453 1171 AlwNI CAG_nnn'CTG Cuts at: 4367 5730 7302 4367 Size: 1363 1572 5052 Fragments arranged by size: 5052 1572 1363 ApaLI G'TGCA_C Cuts at: 5153 6044 7200 5153 Size: 891 1156 5940 Fragments arranged by size: 5940 1156 891 ApoI r'AATT_y Cuts at: 1493 2818 3177 3227 4012 5050 5296 5522 5674 Size: 1325 359 50 785 1038 246 226 152 Cuts at: 5674 7783 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1325 1038 785 359 246 226 152 50 AseI AT'TA_AT Cuts at: 160 1032 6541 160 Size: 872 5509 1606 Fragments arranged by size: 5509 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7987 AvaI C'yCGr_G Cuts at: 1870 1943 2095 5312 1870 Size: 73 152 3217 4545 Fragments arranged by size: 4545 3217 152 73 BamHI G'GATC_C Cuts at: 2884 4832 5317 5684 2884 Size: 1948 485 367 5187 Fragments arranged by size: 5187 1948 485 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3863 3900 3931 5308 6763 Size: 325 217 627 2076 37 31 1377 1455 Cuts at: 6763 618 Size: 1842 Fragments arranged by size: 2076 1842 1455 1377 627 325 217 37 31 BanII G_rGCy'C Cuts at: 1391 2374 5306 1391 Size: 983 2932 4072 Fragments arranged by size: 4072 2932 983 BbeI G_GCGC'C Cuts at: 3935 3935 Size: 7987 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2811 4338 928 Size: 971 912 1527 4577 Fragments arranged by size: 4577 1527 971 912 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6178 6212 1903 Size: 34 4241 34 3678 Fragments arranged by size: 4241 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4495 5879 7089 4495 Size: 1384 1210 5393 Fragments arranged by size: 5393 1384 1210 BclI T'GATC_A Cuts at: 1548 2839 3156 3697 1548 Size: 1291 317 541 5838 Fragments arranged by size: 5838 1291 541 317 BfrBI ATG'CAT Cuts at: 2231 4795 2231 Size: 2564 5423 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3336 6598 136 Size: 107 122 71 631 605 1664 3262 1525 Fragments arranged by size: 3262 1664 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2140 2662 4462 7982 2140 Size: 522 1800 3520 2145 Fragments arranged by size: 3520 2145 1800 522 BlpI GC'TnA_GC Cuts at: 2964 5404 2964 Size: 2440 5547 Bme1580I G_kGCm'C Cuts at: 1790 5157 6048 7204 1790 Size: 3367 891 1156 2573 Fragments arranged by size: 3367 2573 1156 891 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7987 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3664 3906 4475 Size: 416 53 83 399 24 2247 242 569 Cuts at: 4475 5718 6676 442 Size: 1243 958 1753 Fragments arranged by size: 2247 1753 1243 958 569 416 399 242 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7987 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2095 4072 4180 2095 Size: 1977 108 5902 Fragments arranged by size: 5902 1977 108 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4150 4476 6647 1239 Size: 2911 326 2171 2579 Fragments arranged by size: 2911 2579 2171 326 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3845 4841 6112 6977 7275 3845 Size: 996 1271 865 298 4557 Fragments arranged by size: 4557 1271 996 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3711 4708 6650 882 Size: 617 2212 997 1942 2219 Fragments arranged by size: 2219 2212 1942 997 617 BsaAI yAC'GTr Cuts at: 493 1204 3327 4618 5778 493 Size: 711 2123 1291 1160 2702 Fragments arranged by size: 2702 2123 1291 1160 711 BsaBI GATnn'nnATC Cuts at: 2838 2889 3846 7981 2838 Size: 51 957 4135 2844 Fragments arranged by size: 4135 2844 957 51 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3932 5794 6176 6811 Size: 53 83 186 936 2399 1862 382 635 Cuts at: 6811 275 Size: 1451 Fragments arranged by size: 2399 1862 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2039 6420 6818 7092 7239 7650 2039 Size: 4381 398 274 147 411 2376 Fragments arranged by size: 4381 2376 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3038 3068 1178 Size: 30 165 30 1635 30 6097 Fragments arranged by size: 6097 1635 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2058 4064 4153 4425 1186 Size: 872 2006 89 272 4748 Fragments arranged by size: 4748 2006 872 272 89 BseYI C'CCAG_C Cuts at: 2062 3988 5208 7190 7686 2062 Size: 1926 1220 1982 496 2363 Fragments arranged by size: 2363 1982 1926 1220 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3071 3408 3798 5025 5124 1163 Size: 573 1335 337 390 1227 99 4026 Fragments arranged by size: 4026 1335 1227 573 390 337 99 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5437 6198 7226 1540 Size: 275 186 3436 761 1028 2301 Fragments arranged by size: 3436 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2374 5157 6048 6133 7204 2374 Size: 2783 891 85 1071 3157 Fragments arranged by size: 3157 2783 1071 891 85 BsmI GAATG_Cn' Cuts at: 2515 2944 5498 5591 2515 Size: 429 2554 93 4911 Fragments arranged by size: 4911 2554 429 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2374 5157 5306 6048 6133 7204 1391 Size: 399 584 2783 149 742 85 1071 2174 Fragments arranged by size: 2783 2174 1071 742 584 399 149 85 BspEI T'CCGG_A Cuts at: 2039 7650 2039 Size: 5611 2376 BspHI T'CATG_A Cuts at: 2454 5876 7606 2454 Size: 3422 1730 2835 Fragments arranged by size: 3422 2835 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2716 3430 5342 844 Size: 1872 714 1912 3489 Fragments arranged by size: 3489 1912 1872 714 BsrBI CCG'CTC Cuts at: 1394 1823 5874 1394 Size: 429 4051 3507 Fragments arranged by size: 4051 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4147 5034 6482 6656 65 Size: 1183 2899 887 1448 174 1396 Fragments arranged by size: 2899 1448 1396 1183 887 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6631 6818 1700 Size: 110 4821 187 2869 Fragments arranged by size: 4821 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7987 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7987 BssSI C'ACGA_G Cuts at: 6047 7059 6047 Size: 1012 6975 BstAPI GCAn_nnn'nTGC Cuts at: 794 2519 4999 794 Size: 1725 2480 3782 Fragments arranged by size: 3782 2480 1725 BstBI TT'CG_AA Cuts at: 3949 6791 3949 Size: 2842 5145 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7987 BstXI CCAn_nnnn'nTGG Cuts at: 3310 3463 3310 Size: 153 7834 BstYI r'GATC_y Cuts at: 1972 2140 2335 2662 2884 4462 4832 5317 5684 Size: 168 195 327 222 1578 370 485 367 Cuts at: 5684 6069 6086 7538 7624 7636 7982 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1578 1452 485 385 370 367 346 327 222 195 168 86 17 12 BstZ17I GTA'TAC Cuts at: 2877 2877 Size: 7987 Bsu36I CC'TnA_GG Cuts at: 4827 4827 Size: 7987 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7435 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7248 Fragments arranged by size: 7248 683 56 BtsI GCAGTG_nn' Cuts at: 2626 3818 4078 4159 5503 6297 6317 2626 Size: 1192 260 81 1344 794 20 4296 Fragments arranged by size: 4296 1344 1192 794 260 81 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7987 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4477 4512 529 Size: 35 3913 35 4004 Fragments arranged by size: 4004 3913 35 35 DraI TTT'AAA Cuts at: 3838 5291 3838 Size: 1453 6534 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6994 809 Size: 507 5678 1802 Fragments arranged by size: 5678 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2265 3453 3920 Size: 54 1425 50 275 18 435 1188 467 Cuts at: 3920 4962 5116 5434 6323 7952 8 Size: 1042 154 318 889 1629 43 Fragments arranged by size: 1629 1425 1188 1042 889 467 435 318 275 154 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5434 1537 Size: 275 3622 4090 Fragments arranged by size: 4090 3622 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2709 3116 3222 4041 4063 4384 4573 5917 Size: 1356 407 106 819 22 321 189 1344 Cuts at: 5917 1353 Size: 3423 Fragments arranged by size: 3423 1356 1344 819 407 321 189 106 22 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6574 6948 7094 387 Size: 1064 138 4985 374 146 1280 Fragments arranged by size: 4985 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2372 2372 Size: 7987 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2343 2396 2912 3015 3140 3188 4150 Size: 393 711 53 516 103 125 48 962 Cuts at: 4150 4281 4476 4554 5210 5285 6050 6647 7434 Size: 131 195 78 656 75 765 597 787 Cuts at: 7434 7702 1239 Size: 268 1524 Fragments arranged by size: 1524 962 787 765 711 656 597 516 393 268 195 131 125 103 78 75 53 48 EcoNI CCTnn'n_nnAGG Cuts at: 5279 5279 Size: 7987 EcoO109I rG'GnC_Cy Cuts at: 2100 2380 2100 Size: 280 7707 EcoRI G'AATT_C Cuts at: 4012 5296 5674 4012 Size: 1284 378 6325 Fragments arranged by size: 6325 1284 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4695 4695 Size: 7987 FspI TGC'GCA Cuts at: 6493 6493 Size: 7987 HaeII r_GCGC'y Cuts at: 1678 2409 3935 7134 1678 Size: 731 1526 3199 2531 Fragments arranged by size: 3199 2531 1526 731 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3601 3633 4073 4105 4337 Size: 32 135 32 2029 32 440 32 232 Cuts at: 4337 4369 4522 4554 6628 6660 6702 6734 1373 Size: 32 153 32 2074 32 42 32 2626 Fragments arranged by size: 2626 2074 2029 440 232 153 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3141 4560 5331 5577 669 Size: 988 281 1203 1419 771 246 3079 Fragments arranged by size: 3079 1419 1203 988 771 281 246 HindIII A'AGCT_T Cuts at: 748 2475 3170 3574 748 Size: 1727 695 404 5161 Fragments arranged by size: 5161 1727 695 404 HpaI GTT'AAC Cuts at: 5577 5577 Size: 7987 KasI G'GCGC_C Cuts at: 3931 3931 Size: 7987 KpnI G_GTAC'C Cuts at: 3904 5312 3904 Size: 1408 6579 MfeI C'AATT_G Cuts at: 5586 5586 Size: 7987 MluI A'CGCG_T Cuts at: 7863 7863 Size: 7987 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3173 3381 4921 7101 7285 1305 Size: 1868 208 1540 2180 184 2007 Fragments arranged by size: 2180 2007 1868 1540 208 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2267 3455 3922 4964 5118 Size: 54 1425 343 435 1188 467 1042 154 Cuts at: 5118 10 Size: 2879 Fragments arranged by size: 2879 1425 1188 1042 467 435 343 154 54 MslI CAynn'nnrTG Cuts at: 518 1166 4790 5945 6304 6463 518 Size: 648 3624 1155 359 159 2042 Fragments arranged by size: 3624 2042 1155 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2968 5403 6080 7228 7473 1606 Size: 1362 2435 677 1148 245 2120 Fragments arranged by size: 2435 2120 1362 1148 677 245 NarI GG'CG_CC Cuts at: 3932 3932 Size: 7987 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7435 NdeI CA'TA_TG Cuts at: 387 2497 3450 387 Size: 2110 953 4924 Fragments arranged by size: 4924 2110 953 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7987 NotI GC'GGCC_GC Cuts at: 5434 5434 Size: 7987 NsiI A_TGCA'T Cuts at: 2233 4797 2233 Size: 2564 5423 NspI r_CATG'y Cuts at: 1132 1229 2746 4799 5345 6890 1132 Size: 97 1517 2053 546 1545 2229 Fragments arranged by size: 2229 2053 1545 1517 546 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7987 PciI A'CATG_T Cuts at: 6886 6886 Size: 7987 PflMI CCAn_nnn'nTGG Cuts at: 1108 4940 1108 Size: 3832 4155 PfoI T'CCnGG_A Cuts at: 3462 3462 Size: 7987 PmeI GTTT'AAAC Cuts at: 5291 5291 Size: 7987 PmlI CAC'GTG Cuts at: 3327 4618 3327 Size: 1291 6696 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6056 6088 7569 7601 6056 Size: 32 1481 32 6442 Fragments arranged by size: 6442 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2380 2380 Size: 7987 PshAI GACnn'nnGTC Cuts at: 1613 4195 1613 Size: 2582 5405 PsiI TTA'TAA Cuts at: 3398 5557 3398 Size: 2159 5828 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4423 4455 4938 4970 4423 Size: 32 483 32 7440 Fragments arranged by size: 7440 483 32 32 PstI C_TGCA'G Cuts at: 830 3609 5339 830 Size: 2779 1730 3478 Fragments arranged by size: 3478 2779 1730 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7987 PvuII CAG'CTG Cuts at: 2968 2968 Size: 7987 SacI G_AGCT'C Cuts at: 2374 2374 Size: 7987 SalI G'TCGA_C Cuts at: 1936 5329 1936 Size: 3393 4594 SbfI CC_TGCA'GG Cuts at: 5339 5339 Size: 7987 ScaI AGT'ACT Cuts at: 4588 6235 7947 4588 Size: 1647 1712 4628 Fragments arranged by size: 4628 1712 1647 SexAI A'CCwGG_T Cuts at: 2307 2307 Size: 7987 SfcI C'TryA_G Cuts at: 826 1045 3285 3605 4779 5202 5335 6470 7151 Size: 219 2240 320 1174 423 133 1135 681 Cuts at: 7151 7342 826 Size: 191 1471 Fragments arranged by size: 2240 1471 1174 1135 681 423 320 219 191 133 SfoI GGC'GCC Cuts at: 3933 3933 Size: 7987 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7987 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7987 SmaI CCC'GGG Cuts at: 1872 5314 1872 Size: 3442 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3824 4820 6091 6992 7254 820 Size: 197 926 1881 996 1271 901 262 1553 Fragments arranged by size: 1881 1553 1271 996 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5778 493 Size: 5285 2702 SpeI A'CTAG_T Cuts at: 152 2701 3019 3581 152 Size: 2549 318 562 4558 Fragments arranged by size: 4558 2549 562 318 SphI G_CATG'C Cuts at: 1132 5345 1132 Size: 4213 3774 SspI AAT'ATT Cuts at: 5 52 2278 3795 4927 5911 5 Size: 47 2226 1517 1132 984 2081 Fragments arranged by size: 2226 2081 1517 1132 984 47 StuI AGG'CCT Cuts at: 1517 4203 1517 Size: 2686 5301 StyI C'CwwG_G Cuts at: 513 1065 2780 4902 5426 513 Size: 552 1715 2122 524 3074 Fragments arranged by size: 3074 2122 1715 552 524 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6014 6031 6184 6369 6814 325 Size: 1255 4434 17 153 185 445 1498 Fragments arranged by size: 4434 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3672 4586 6233 Size: 274 80 33 51 1443 1695 914 1647 Cuts at: 6233 7945 96 Size: 1712 138 Fragments arranged by size: 1712 1695 1647 1443 914 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2776 2808 7947 7979 2776 Size: 32 5139 32 2784 Fragments arranged by size: 5139 2784 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7987 XbaI T'CTAG_A Cuts at: 4459 5323 4459 Size: 864 7123 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7987 XmaI C'CCGG_G Cuts at: 1870 5312 1870 Size: 3442 4545 XmnI GAAnn'nnTTC Cuts at: 2241 2586 3125 3531 6116 2241 Size: 345 539 406 2585 4112 Fragments arranged by size: 4112 2585 539 406 345 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5795 276 Size: 53 83 186 936 4261 2468 Fragments arranged by size: 4261 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI Bpu10I BsiWI BsmBI DraIII EcoRV FseI FspAI NaeI NgoMIV NruI PacI PspOMI PspXI RsrII SacII SanDI SapI SfiI SrfI SwaI XcmI