(Linear) (Six-base) MAPSORT of: KIEE4590.seq Check: 602 from: 1 to: 3282 KIEE4590.orf With 182 enzymes: SgfI * July 3, 2012 19:01 .. AccI GT'mk_AC Cuts at: 0 871 3282 Size: 871 2411 Acc65I G'GTAC_C Cuts at: 0 1895 3282 Size: 1895 1387 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 338 391 907 1010 1135 1183 2276 2549 Size: 338 53 516 103 125 48 1093 273 Cuts at: 2549 3205 3280 3282 Size: 656 75 2 Fragments arranged by size: 1093 656 516 338 273 125 103 75 53 48 2 AfeI AGC'GCT Cuts at: 0 402 3282 Size: 402 2880 AflIII A'CryG_T Cuts at: 0 1319 3282 Size: 1319 1963 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 350 2803 3282 Size: 350 2453 479 Fragments arranged by size: 2453 479 350 AlwNI CAG_nnn'CTG Cuts at: 0 2362 3282 Size: 2362 920 ApaLI G'TGCA_C Cuts at: 0 3148 3282 Size: 3148 134 ApoI r'AATT_y Cuts at: 0 813 1172 1222 2007 3045 3282 Size: 813 359 50 785 1038 237 Fragments arranged by size: 1038 813 785 359 237 50 AvaI C'yCGr_G Cuts at: 0 90 3282 Size: 90 3192 BamHI G'GATC_C Cuts at: 0 879 2827 3282 Size: 879 1948 455 Fragments arranged by size: 1948 879 455 BanI G'GyrC_C Cuts at: 0 1858 1895 1926 3282 Size: 1858 37 31 1356 Fragments arranged by size: 1858 1356 37 31 BanII G_rGCy'C Cuts at: 0 369 3282 Size: 369 2913 BbeI G_GCGC'C Cuts at: 0 1930 3282 Size: 1930 1352 BbsI GAAGACnn'nnnn_ Cuts at: 0 806 2333 3282 Size: 806 1527 949 Fragments arranged by size: 1527 949 806 BciVI GTATCCnnnnn_n' Cuts at: 0 2490 3282 Size: 2490 792 BclI T'GATC_A Cuts at: 0 834 1151 1692 3282 Size: 834 317 541 1590 Fragments arranged by size: 1590 834 541 317 BfrBI ATG'CAT Cuts at: 0 226 2790 3282 Size: 226 2564 492 Fragments arranged by size: 2564 492 226 BglI GCCn_nnn'nGGC Cuts at: 0 1331 3282 Size: 1331 1951 BglII A'GATC_T Cuts at: 0 135 657 2457 3282 Size: 135 522 1800 825 Fragments arranged by size: 1800 825 522 135 BlpI GC'TnA_GC Cuts at: 0 959 3282 Size: 959 2323 Bme1580I G_kGCm'C Cuts at: 0 3152 3282 Size: 3152 130 BmrI ACTGGGnnnn_n' Cuts at: 0 1659 1901 2470 3282 Size: 1659 242 569 812 Fragments arranged by size: 1659 812 569 242 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 90 2067 2175 3282 Size: 90 1977 108 1107 Fragments arranged by size: 1977 1107 108 90 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2145 2471 3282 Size: 2145 326 811 Fragments arranged by size: 2145 811 326 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1840 2836 3282 Size: 1840 996 446 Fragments arranged by size: 1840 996 446 BsaI GGTCTCn'nnnn_ Cuts at: 0 1706 2703 3282 Size: 1706 997 579 Fragments arranged by size: 1706 997 579 BsaAI yAC'GTr Cuts at: 0 1322 2613 3282 Size: 1322 1291 669 Fragments arranged by size: 1322 1291 669 BsaBI GATnn'nnATC Cuts at: 0 833 884 1841 3282 Size: 833 51 957 1441 Fragments arranged by size: 1441 957 833 51 BsaHI Gr'CG_yC Cuts at: 0 1927 3282 Size: 1927 1355 BsaWI w'CCGG_w Cuts at: 0 34 3282 Size: 34 3248 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1033 1063 3282 Size: 1033 30 2219 Fragments arranged by size: 2219 1033 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 53 2059 2148 2420 3282 Size: 53 2006 89 272 862 Fragments arranged by size: 2006 862 272 89 53 BseYI C'CCAG_C Cuts at: 0 57 1983 3203 3282 Size: 57 1926 1220 79 Fragments arranged by size: 1926 1220 79 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1066 1403 1793 3020 3119 3282 Size: 1066 337 390 1227 99 163 Fragments arranged by size: 1227 1066 390 337 163 99 BsiHKAI G_wGCw'C Cuts at: 0 369 3152 3282 Size: 369 2783 130 Fragments arranged by size: 2783 369 130 BsmI GAATG_Cn' Cuts at: 0 510 939 3282 Size: 510 429 2343 Fragments arranged by size: 2343 510 429 Bsp1286I G_dGCh'C Cuts at: 0 369 3152 3282 Size: 369 2783 130 Fragments arranged by size: 2783 369 130 BspEI T'CCGG_A Cuts at: 0 34 3282 Size: 34 3248 BspHI T'CATG_A Cuts at: 0 449 3282 Size: 449 2833 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 711 1425 3282 Size: 711 714 1857 Fragments arranged by size: 1857 714 711 BsrDI GCAATG_nn' Cuts at: 0 2142 3029 3282 Size: 2142 887 253 Fragments arranged by size: 2142 887 253 BstAPI GCAn_nnn'nTGC Cuts at: 0 514 2994 3282 Size: 514 2480 288 Fragments arranged by size: 2480 514 288 BstBI TT'CG_AA Cuts at: 0 1944 3282 Size: 1944 1338 BstXI CCAn_nnnn'nTGG Cuts at: 0 1305 1458 3282 Size: 1305 153 1824 Fragments arranged by size: 1824 1305 153 BstYI r'GATC_y Cuts at: 0 135 330 657 879 2457 2827 3282 Size: 135 195 327 222 1578 370 455 Fragments arranged by size: 1578 455 370 327 222 195 135 BstZ17I GTA'TAC Cuts at: 0 872 3282 Size: 872 2410 Bsu36I CC'TnA_GG Cuts at: 0 2822 3282 Size: 2822 460 BtsI GCAGTG_nn' Cuts at: 0 621 1813 2073 2154 3282 Size: 621 1192 260 81 1128 Fragments arranged by size: 1192 1128 621 260 81 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2472 2507 3282 Size: 2472 35 775 Fragments arranged by size: 2472 775 35 DraI TTT'AAA Cuts at: 0 1833 3282 Size: 1833 1449 EaeI y'GGCC_r Cuts at: 0 260 1448 1915 2957 3111 3282 Size: 260 1188 467 1042 154 171 Fragments arranged by size: 1188 1042 467 260 171 154 EarI CTCTTCn'nnn_ Cuts at: 0 704 1111 1217 2036 2058 2379 2568 3282 Size: 704 407 106 819 22 321 189 714 Fragments arranged by size: 819 714 704 407 321 189 106 22 EcoICRI GAG'CTC Cuts at: 0 367 3282 Size: 367 2915 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 338 391 907 1010 1135 1183 2145 2276 Size: 338 53 516 103 125 48 962 131 Cuts at: 2276 2471 2549 3205 3280 3282 Size: 195 78 656 75 2 Fragments arranged by size: 962 656 516 338 195 131 125 103 78 75 53 48 2 EcoNI CCTnn'n_nnAGG Cuts at: 0 3274 3282 Size: 3274 8 EcoO109I rG'GnC_Cy Cuts at: 0 95 375 3282 Size: 95 280 2907 Fragments arranged by size: 2907 280 95 EcoRI G'AATT_C Cuts at: 0 2007 3282 Size: 2007 1275 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2690 3282 Size: 2690 592 HaeII r_GCGC'y Cuts at: 0 404 1930 3282 Size: 404 1526 1352 Fragments arranged by size: 1526 1352 404 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1596 1628 2068 2100 2332 2364 2517 2549 Size: 1596 32 440 32 232 32 153 32 Cuts at: 2549 3282 Size: 733 Fragments arranged by size: 1596 733 440 232 153 32 32 32 32 HincII GTy'rAC Cuts at: 0 1136 2555 3282 Size: 1136 1419 727 Fragments arranged by size: 1419 1136 727 HindIII A'AGCT_T Cuts at: 0 470 1165 1569 3282 Size: 470 695 404 1713 Fragments arranged by size: 1713 695 470 404 KasI G'GCGC_C Cuts at: 0 1926 3282 Size: 1926 1356 KpnI G_GTAC'C Cuts at: 0 1899 3282 Size: 1899 1383 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1168 1376 2916 3282 Size: 1168 208 1540 366 Fragments arranged by size: 1540 1168 366 208 MscI TGG'CCA Cuts at: 0 262 1450 1917 2959 3113 3282 Size: 262 1188 467 1042 154 169 Fragments arranged by size: 1188 1042 467 262 169 154 MslI CAynn'nnrTG Cuts at: 0 2785 3282 Size: 2785 497 MspA1I CmG'CkG Cuts at: 0 963 3282 Size: 963 2319 NarI GG'CG_CC Cuts at: 0 1927 3282 Size: 1927 1355 NdeI CA'TA_TG Cuts at: 0 492 1445 3282 Size: 492 953 1837 Fragments arranged by size: 1837 953 492 NsiI A_TGCA'T Cuts at: 0 228 2792 3282 Size: 228 2564 490 Fragments arranged by size: 2564 490 228 NspI r_CATG'y Cuts at: 0 741 2794 3282 Size: 741 2053 488 Fragments arranged by size: 2053 741 488 PflMI CCAn_nnn'nTGG Cuts at: 0 2935 3282 Size: 2935 347 PfoI T'CCnGG_A Cuts at: 0 1457 3282 Size: 1457 1825 PmlI CAC'GTG Cuts at: 0 1322 2613 3282 Size: 1322 1291 669 Fragments arranged by size: 1322 1291 669 PpuMI rG'GwC_Cy Cuts at: 0 375 3282 Size: 375 2907 PshAI GACnn'nnGTC Cuts at: 0 2190 3282 Size: 2190 1092 PsiI TTA'TAA Cuts at: 0 1393 3282 Size: 1393 1889 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2418 2450 2933 2965 3282 Size: 2418 32 483 32 317 Fragments arranged by size: 2418 483 317 32 32 PstI C_TGCA'G Cuts at: 0 1604 3282 Size: 1604 1678 PvuII CAG'CTG Cuts at: 0 963 3282 Size: 963 2319 SacI G_AGCT'C Cuts at: 0 369 3282 Size: 369 2913 ScaI AGT'ACT Cuts at: 0 2583 3282 Size: 2583 699 SexAI A'CCwGG_T Cuts at: 0 302 3282 Size: 302 2980 SfcI C'TryA_G Cuts at: 0 1280 1600 2774 3197 3282 Size: 1280 320 1174 423 85 Fragments arranged by size: 1280 1174 423 320 85 SfoI GGC'GCC Cuts at: 0 1928 3282 Size: 1928 1354 SmlI C'TyrA_G Cuts at: 0 1819 2815 3282 Size: 1819 996 467 Fragments arranged by size: 1819 996 467 SpeI A'CTAG_T Cuts at: 0 696 1014 1576 3282 Size: 696 318 562 1706 Fragments arranged by size: 1706 696 562 318 SspI AAT'ATT Cuts at: 0 273 1790 2922 3282 Size: 273 1517 1132 360 Fragments arranged by size: 1517 1132 360 273 StuI AGG'CCT Cuts at: 0 2198 3282 Size: 2198 1084 StyI C'CwwG_G Cuts at: 0 775 2897 3282 Size: 775 2122 385 Fragments arranged by size: 2122 775 385 TatI w'GTAC_w Cuts at: 0 1667 2581 3282 Size: 1667 914 701 Fragments arranged by size: 1667 914 701 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 771 803 3282 Size: 771 32 2479 Fragments arranged by size: 2479 771 32 XbaI T'CTAG_A Cuts at: 0 2454 3282 Size: 2454 828 XmnI GAAnn'nnTTC Cuts at: 0 236 581 1120 1526 3282 Size: 236 345 539 406 1756 Fragments arranged by size: 1756 539 406 345 236 Enzymes that do cut: AccI Acc65I AcuI AfeI AflIII AlfI AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtsI CspCI DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI MslI MspA1I NarI NdeI NsiI NspI PflMI PfoI PmlI PpuMI PshAI PsiI PsrI PstI PvuII SacI ScaI SexAI SfcI SfoI SmlI SpeI SspI StuI StyI TatI TstI XbaI XmnI Enzymes that do not cut: AarI AatII AclI AflII AgeI AhdI AleI AloI ApaI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BmgBI BmtI Bpu10I BsiEI BsiWI BsmBI BsrBI BsrFI BsrGI BssHII BssSI BstEII BtgI BtgZI ClaI DraIII DrdI EagI EciI EcoRV FseI FspI FspAI HpaI MfeI MluI NaeI NcoI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PpiI PspOMI PspXI PvuI RsrII SacII SalI SanDI SapI SbfI SfiI SgfI SgrAI SmaI SnaBI SphI SrfI SwaI TaqII Tth111I XcmI XhoI XmaI ZraI