################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## Acc65I AccIII AflII AgeI AhdI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbrPI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaBI BseAI BsiWI BsmI BspEI BspHI BssHII Bst1107I BstEII BstPI BstZ17I CelII CpoI EagI Eam1105I EarI EclXI Eco52I EcoO65I EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MamI MluI MroI NaeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsrII SacII SalI SapI ScaI SfiI SgrAI SmlI SnaBI SpeI SspI StuI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccII 1716 CGCG CG^CG AclI 1979 AACGTT AA^CGTT AocI 3493 CCTCAGG CC^T[ACGT]AGG BalI 2849 TGGCCA TGG^CCA BbeI 2617 GGCGCC GGCGC^C BbsI 2434 GAAGAC GAAGAC(2/6) BcgI 3278 CGACAACGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 52 CTAG C^TAG BglII 995 AGATCT A^GATCT BpuAI 2434 GAAGAC GAAGAC(2/6) BsaAI 2491 TACGTG [CT]AC^GT[GA] BsiEI 3194 CGGTCG CG[GA][CT]^CG BsmBI 2649 CGTCTC CGTCTC(1/5) Bsp1407I 3380 TGTACA T^GTACA BspDI 466 ATCGAT AT^CGAT BsrBI 1764 GAGCGG CCGCTC(-3/-3) BsrFI 2675 ACCGGC [GA]^CCGG[CT] BsrGI 3380 TGTACA T^GTACA BssSI 340 CACGAG CACGAG(-5/-1) BstAPI 2579 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1039 TTCGAA TT^CGAA BstUI 1716 CGCG CG^CG Bsu36I 3493 CCTCAGG CC^T[ACGT]AGG Cfr10I 2675 ACCGGC [GA]^CCGG[CT] ClaI 466 ATCGAT AT^CGAT DraI 940 TTTAAA TTT^AAA DraIII 2129 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 3493 CCTCAGG CC^T[ACGT]AGG EcoRI 613 GAATTC G^AATTC KasI 2617 GGCGCC G^GCGCC MaeI 52 CTAG C^TAG MfeI 854 CAATTG C^AATTG MluNI 2849 TGGCCA TGG^CCA MscI 2849 TGGCCA TGG^CCA MunI 854 CAATTG C^AATTG MvnI 1716 CGCG CG^CG NarI 2617 GGCGCC GG^CGCC NcoI 821 CCATGG C^CATGG NdeI 728 CATATG CA^TATG NspV 1039 TTCGAA TT^CGAA Psp1406I 1979 AACGTT AA^CGTT SbfI 240 CCTGCAGG CCTGCA^GG SfoI 2617 GGCGCC GGC^GCC SfuI 1039 TTCGAA TT^CGAA SmaI 1817 CCCGGG CCC^GGG SphI 1871 GCATGC GCATG^C Sse8387I 240 CCTGCAGG CCTGCA^GG SspBI 3380 TGTACA T^GTACA XmaCI 1817 CCCGGG C^CCGGG XmaI 1817 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AatII 2647 GACGTC GACGT^C 3097 GACGTC Alw44I 334 GTGCAC G^TGCAC 706 GTGCAC AlwI 1893 GATCC GGATC(4/5) 1712 GGATC Aor51HI 100 AGCGCT AGC^GCT 1069 AGCGCT ApaLI 334 GTGCAC G^TGCAC 706 GTGCAC BbvCI 636 CCTCAGC CCTCAGC(-5/-2) 3150 CCTCAGC BsaWI 367 ACCGGA [AT]^CCGG[AT] 2090 ACCGGA BsgI 2530 CTGCAC GTGCAG(16/14) 1249 GTGCAG BstXI 183 CCAAACGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1223 CCACCGAGCTGG BstYI 995 AGATCT [GA]^GATC[CT] 1892 AGATCC DrdI 115 GACAGCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2890 GACCTGCCTGTC Eco47III 100 AGCGCT AGC^GCT 1069 AGCGCT EcoNI 1487 CCTTGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1779 CCTCACCCAGG MflI 995 AGATCT [GA]^GATC[CT] 1892 AGATCC MseI 901 TTAA T^TAA 941 TTAA PpuMI 1680 GGGACCC [GA]G^G[AT]CC[CT] 2888 GGGACCT SacI 2749 GAGCTC GAGCT^C 3269 GAGCTC TfiI 2591 GAATC G^A[AT]TC 3048 GAATC Tru9I 901 TTAA T^TAA 941 TTAA XhoII 995 AGATCT [GA]^GATC[CT] 1892 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AflIII AluI AlwNI ApaI ApoI AspHI AspI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrDI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI StyI TaqI TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI