(Linear) (Six-base) MAPSORT of: KIEE1504.seq Check: 6588 from: 1 to: 3507 KIEE1504.orf With 182 enzymes: SgfI * July 15, 2010 18:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 234 1914 3507 Size: 234 1680 1593 Fragments arranged by size: 1680 1593 234 AatII G_ACGT'C Cuts at: 0 2651 3101 3507 Size: 2651 450 406 Fragments arranged by size: 2651 450 406 AccI GT'mk_AC Cuts at: 0 292 1400 1658 2771 3507 Size: 292 1108 258 1113 736 Fragments arranged by size: 1113 1108 736 292 258 AclI AA'CG_TT Cuts at: 0 1980 3507 Size: 1980 1527 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1006 1050 2143 2220 2337 3093 3160 3507 Size: 1006 44 1093 77 117 756 67 347 Fragments arranged by size: 1093 1006 756 347 117 77 67 44 AfeI AGC'GCT Cuts at: 0 102 1071 3507 Size: 102 969 2436 Fragments arranged by size: 2436 969 102 AflIII A'CryG_T Cuts at: 0 266 356 1175 2539 3507 Size: 266 90 819 1364 968 Fragments arranged by size: 1364 968 819 266 90 AleI CACnn'nnGTG Cuts at: 0 1312 2212 3229 3507 Size: 1312 900 1017 278 Fragments arranged by size: 1312 1017 900 278 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 442 474 3507 Size: 442 32 3033 Fragments arranged by size: 3033 442 32 AlwNI CAG_nnn'CTG Cuts at: 0 315 1257 1761 1944 2827 3022 3507 Size: 315 942 504 183 883 195 485 Fragments arranged by size: 942 883 504 485 315 195 183 ApaI G_GGCC'C Cuts at: 0 391 738 1272 1440 1629 1818 3507 Size: 391 347 534 168 189 189 1689 Fragments arranged by size: 1689 534 391 347 189 189 168 ApaLI G'TGCA_C Cuts at: 0 334 706 3507 Size: 334 372 2801 Fragments arranged by size: 2801 372 334 ApoI r'AATT_y Cuts at: 0 586 613 755 885 3507 Size: 586 27 142 130 2622 Fragments arranged by size: 2622 586 142 130 27 AvaI C'yCGr_G Cuts at: 0 1460 1817 2116 2157 3507 Size: 1460 357 299 41 1350 Fragments arranged by size: 1460 1350 357 299 41 BanI G'GyrC_C Cuts at: 0 220 246 1132 2569 2617 2916 3507 Size: 220 26 886 1437 48 299 591 Fragments arranged by size: 1437 886 591 299 220 48 26 BanII G_rGCy'C Cuts at: 0 159 233 391 738 1162 1272 1440 1629 Size: 159 74 158 347 424 110 168 189 Cuts at: 1629 1723 1818 2753 3273 3507 Size: 94 95 935 520 234 Fragments arranged by size: 935 520 424 347 234 189 168 159 158 110 95 94 74 BbeI G_GCGC'C Cuts at: 0 2621 3507 Size: 2621 886 BbsI GAAGACnn'nnnn_ Cuts at: 0 2441 3507 Size: 2441 1066 BbvCI CC'TCA_GC Cuts at: 0 637 3151 3507 Size: 637 2514 356 Fragments arranged by size: 2514 637 356 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3267 3301 3507 Size: 3267 34 206 Fragments arranged by size: 3267 206 34 BglII A'GATC_T Cuts at: 0 995 3507 Size: 995 2512 Bme1580I G_kGCm'C Cuts at: 0 251 338 391 436 710 738 1272 1440 Size: 251 87 53 45 274 28 534 168 Cuts at: 1440 1629 1818 2572 3507 Size: 189 189 754 935 Fragments arranged by size: 935 754 534 274 251 189 189 168 87 53 45 28 BmgBI CAC'GTC Cuts at: 0 1614 3507 Size: 1614 1893 BmrI ACTGGGnnnn_n' Cuts at: 0 552 2548 3507 Size: 552 1996 959 Fragments arranged by size: 1996 959 552 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1148 1719 2416 2595 2767 3310 3507 Size: 1148 571 697 179 172 543 197 Fragments arranged by size: 1148 697 571 543 197 179 172 Bpu10I CC'TnA_GC Cuts at: 0 39 474 637 1837 3151 3507 Size: 39 435 163 1200 1314 356 Fragments arranged by size: 1314 1200 435 356 163 39 BsaI GGTCTCn'nnnn_ Cuts at: 0 540 1012 1935 2666 2967 3507 Size: 540 472 923 731 301 540 Fragments arranged by size: 923 731 540 540 472 301 BsaAI yAC'GTr Cuts at: 0 2493 3507 Size: 2493 1014 BsaHI Gr'CG_yC Cuts at: 0 289 2618 2648 3098 3507 Size: 289 2329 30 450 409 Fragments arranged by size: 2329 450 409 289 30 BsaWI w'CCGG_w Cuts at: 0 367 2090 3507 Size: 367 1723 1417 Fragments arranged by size: 1723 1417 367 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 104 134 2929 2959 3088 3118 3507 Size: 104 30 2795 30 129 30 389 Fragments arranged by size: 2795 389 129 104 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 13 88 477 1722 2845 2985 3136 3439 Size: 13 75 389 1245 1123 140 151 303 Cuts at: 3439 3507 Size: 68 Fragments arranged by size: 1245 1123 389 303 151 140 75 68 13 BseYI C'CCAG_C Cuts at: 0 103 308 395 550 1581 1684 1724 3015 Size: 103 205 87 155 1031 103 40 1291 Cuts at: 3015 3161 3239 3469 3507 Size: 146 78 230 38 Fragments arranged by size: 1291 1031 230 205 155 146 103 103 87 78 40 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1270 2515 3507 Size: 1270 1245 992 Fragments arranged by size: 1270 1245 992 BsiEI CG_ry'CG Cuts at: 0 3197 3507 Size: 3197 310 BsiHKAI G_wGCw'C Cuts at: 0 338 710 1832 1856 2581 2753 2995 3273 Size: 338 372 1122 24 725 172 242 278 Cuts at: 3273 3507 Size: 234 Fragments arranged by size: 1122 725 372 338 278 242 234 172 24 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2655 3507 Size: 2655 852 Bsp1286I G_dGCh'C Cuts at: 0 159 233 251 338 391 436 710 738 Size: 159 74 18 87 53 45 274 28 Cuts at: 738 1162 1272 1440 1629 1723 1818 1832 1856 Size: 424 110 168 189 94 95 14 24 Cuts at: 1856 2572 2581 2753 2995 3273 3507 Size: 716 9 172 242 278 234 Fragments arranged by size: 716 424 278 274 242 234 189 172 168 159 110 95 94 87 74 53 45 28 24 18 14 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 234 1893 1914 2000 2156 2252 2900 3507 Size: 234 1659 21 86 156 96 648 607 Fragments arranged by size: 1659 648 607 234 156 96 86 21 BsrBI CCG'CTC Cuts at: 0 1766 3507 Size: 1766 1741 BsrDI GCAATG_nn' Cuts at: 0 908 1330 3068 3507 Size: 908 422 1738 439 Fragments arranged by size: 1738 908 439 422 BsrFI r'CCGG_y Cuts at: 0 2675 3507 Size: 2675 832 BsrGI T'GTAC_A Cuts at: 0 3380 3507 Size: 3380 127 BssSI C'ACGA_G Cuts at: 0 340 3507 Size: 340 3167 BstAPI GCAn_nnn'nTGC Cuts at: 0 2585 3507 Size: 2585 922 BstBI TT'CG_AA Cuts at: 0 1040 3507 Size: 1040 2467 BstXI CCAn_nnnn'nTGG Cuts at: 0 190 1230 3507 Size: 190 1040 2277 Fragments arranged by size: 2277 1040 190 BstYI r'GATC_y Cuts at: 0 995 1892 3507 Size: 995 897 1615 Fragments arranged by size: 1615 995 897 Bsu36I CC'TnA_GG Cuts at: 0 3494 3507 Size: 3494 13 BtgI C'CryG_G Cuts at: 0 821 1391 2610 3507 Size: 821 570 1219 897 Fragments arranged by size: 1219 897 821 570 BtsI GCAGTG_nn' Cuts at: 0 2709 3507 Size: 2709 798 ClaI AT'CG_AT Cuts at: 0 467 3507 Size: 467 3040 DraI TTT'AAA Cuts at: 0 942 3507 Size: 942 2565 DraIII CAC_nnn'GTG Cuts at: 0 2134 3507 Size: 2134 1373 DrdI GACnn_nn'nnGTC Cuts at: 0 121 2896 3507 Size: 121 2775 611 Fragments arranged by size: 2775 611 121 EaeI y'GGCC_r Cuts at: 0 683 2049 2258 2849 3507 Size: 683 1366 209 591 658 Fragments arranged by size: 1366 683 658 591 209 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 3157 3507 Size: 3157 350 EcoICRI GAG'CTC Cuts at: 0 2751 3271 3507 Size: 2751 520 236 Fragments arranged by size: 2751 520 236 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1006 1050 1148 1719 2143 2220 2337 2416 Size: 1006 44 98 571 424 77 117 79 Cuts at: 2416 2595 2767 3093 3160 3310 3507 Size: 179 172 326 67 150 197 Fragments arranged by size: 1006 571 424 326 197 179 172 150 117 98 79 77 67 44 EcoNI CCTnn'n_nnAGG Cuts at: 0 1491 1783 3507 Size: 1491 292 1724 Fragments arranged by size: 1724 1491 292 EcoO109I rG'GnC_Cy Cuts at: 0 388 734 1268 1269 1376 1436 1437 1625 Size: 388 346 534 1 107 60 1 188 Cuts at: 1625 1626 1681 1814 2889 3507 Size: 1 55 133 1075 618 Fragments arranged by size: 1075 618 534 388 346 188 133 107 60 55 1 1 1 EcoRI G'AATT_C Cuts at: 0 613 3507 Size: 613 2894 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2754 3507 Size: 2754 753 HaeII r_GCGC'y Cuts at: 0 104 1073 2552 2621 3507 Size: 104 969 1479 69 886 Fragments arranged by size: 1479 969 886 104 69 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 898 930 1673 1705 1755 1787 1838 1870 Size: 898 32 743 32 50 32 51 32 Cuts at: 1870 2096 2128 2852 2884 3088 3120 3507 Size: 226 32 724 32 204 32 387 Fragments arranged by size: 898 743 724 387 226 204 51 50 32 32 32 32 32 32 32 KasI G'GCGC_C Cuts at: 0 2617 3507 Size: 2617 890 MfeI C'AATT_G Cuts at: 0 854 3507 Size: 854 2653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 132 605 843 1979 3507 Size: 132 473 238 1136 1528 Fragments arranged by size: 1528 1136 473 238 132 MscI TGG'CCA Cuts at: 0 2851 3507 Size: 2851 656 MslI CAynn'nnrTG Cuts at: 0 508 820 1174 1312 2212 3229 3507 Size: 508 312 354 138 900 1017 278 Fragments arranged by size: 1017 900 508 354 312 278 138 MspA1I CmG'CkG Cuts at: 0 175 312 663 1710 2520 2585 2745 3092 Size: 175 137 351 1047 810 65 160 347 Cuts at: 3092 3373 3507 Size: 281 134 Fragments arranged by size: 1047 810 351 347 281 175 160 137 134 65 NarI GG'CG_CC Cuts at: 0 2618 3507 Size: 2618 889 NcoI C'CATG_G Cuts at: 0 821 3507 Size: 821 2686 NdeI CA'TA_TG Cuts at: 0 729 3507 Size: 729 2778 NspI r_CATG'y Cuts at: 0 270 360 1179 1875 2543 3507 Size: 270 90 819 696 668 964 Fragments arranged by size: 964 819 696 668 270 90 PasI CC'CwG_GG Cuts at: 0 273 1493 1550 3507 Size: 273 1220 57 1957 Fragments arranged by size: 1957 1220 273 57 PciI A'CATG_T Cuts at: 0 266 356 1175 2539 3507 Size: 266 90 819 1364 968 Fragments arranged by size: 1364 968 819 266 90 PflMI CCAn_nnn'nTGG Cuts at: 0 140 315 1325 1499 3507 Size: 140 175 1010 174 2008 Fragments arranged by size: 2008 1010 175 174 140 PfoI T'CCnGG_A Cuts at: 0 2075 3116 3246 3507 Size: 2075 1041 130 261 Fragments arranged by size: 2075 1041 261 130 PpuMI rG'GwC_Cy Cuts at: 0 1681 2889 3507 Size: 1681 1208 618 Fragments arranged by size: 1681 1208 618 PsiI TTA'TAA Cuts at: 0 806 3507 Size: 806 2701 PspOMI G'GGCC_C Cuts at: 0 387 734 1268 1436 1625 1814 3507 Size: 387 347 534 168 189 189 1693 Fragments arranged by size: 1693 534 387 347 189 189 168 PstI C_TGCA'G Cuts at: 0 245 1745 2590 3507 Size: 245 1500 845 917 Fragments arranged by size: 1500 917 845 245 PvuII CAG'CTG Cuts at: 0 175 312 663 2520 2585 2745 3507 Size: 175 137 351 1857 65 160 762 Fragments arranged by size: 1857 762 351 175 160 137 65 SacI G_AGCT'C Cuts at: 0 2753 3273 3507 Size: 2753 520 234 Fragments arranged by size: 2753 520 234 SanDI GG'GwC_CC Cuts at: 0 1681 3507 Size: 1681 1826 SbfI CC_TGCA'GG Cuts at: 0 245 3507 Size: 245 3262 SexAI A'CCwGG_T Cuts at: 0 1120 1421 2864 3507 Size: 1120 301 1443 643 Fragments arranged by size: 1443 1120 643 301 SfcI C'TryA_G Cuts at: 0 210 241 1741 2586 3507 Size: 210 31 1500 845 921 Fragments arranged by size: 1500 921 845 210 31 SfoI GGC'GCC Cuts at: 0 2619 3507 Size: 2619 888 SmaI CCC'GGG Cuts at: 0 1819 3507 Size: 1819 1688 SphI G_CATG'C Cuts at: 0 1875 3507 Size: 1875 1632 StyI C'CwwG_G Cuts at: 0 821 2027 2657 3507 Size: 821 1206 630 850 Fragments arranged by size: 1206 850 821 630 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 132 409 418 571 1324 1798 1848 2904 Size: 132 277 9 153 753 474 50 1056 Cuts at: 2904 3183 3507 Size: 279 324 Fragments arranged by size: 1056 753 474 324 279 277 153 132 50 9 TatI w'GTAC_w Cuts at: 0 3233 3296 3380 3407 3507 Size: 3233 63 84 27 100 Fragments arranged by size: 3233 100 84 63 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 104 136 2929 2961 3507 Size: 104 32 2793 32 546 Fragments arranged by size: 2793 546 104 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1423 1615 3502 3507 Size: 1423 192 1887 5 Fragments arranged by size: 1887 1423 192 5 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 141 727 2664 2742 3507 Size: 141 586 1937 78 765 Fragments arranged by size: 1937 765 586 141 78 XmaI C'CCGG_G Cuts at: 0 1817 3507 Size: 1817 1690 ZraI GAC'GTC Cuts at: 0 2649 3099 3507 Size: 2649 450 408 Fragments arranged by size: 2649 450 408 Enzymes that do cut: AarI AatII AccI AclI AcuI AfeI AflIII AleI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtsI ClaI DraI DraIII DrdI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I KasI MfeI MmeI MscI MslI MspA1I NarI NcoI NdeI NspI PasI PciI PflMI PfoI PpuMI PsiI PspOMI PstI PvuII SacI SanDI SbfI SexAI SfcI SfoI SmaI SphI StyI TaqII TatI TstI Tth111I XcmI XmaI ZraI Enzymes that do not cut: Acc65I AflII AgeI AhdI AlfI AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BglI BlpI BmtI BplI BpuEI BsaBI BsiWI BsmI BspEI BspHI BssHII BstEII BstZ17I BtgZI CspCI EagI EarI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MluI NaeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PspXI PsrI PvuI RsrII SacII SalI SapI ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI XbaI XhoI XmnI