################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AgeI AhdI AocI ApaI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BbeI BbrPI BcgI BciVI BcnI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsoBI Bsp1407I BspDI BspEI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I FauI FseI FspI HgaI Hin1I KasI KspI MluI MroI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 1555 GGTACC G^GTACC Alw44I 818 GTGCAC G^TGCAC Aor51HI 1286 AGCGCT AGC^GCT ApaLI 818 GTGCAC G^TGCAC Asp718I 1555 GGTACC G^GTACC BalI 470 TGGCCA TGG^CCA BamHI 1379 GGATCC G^GATCC BanI 1555 GGTACC G^G[CT][GA]CC BanII 725 GAGCTC G[GA]GC[CT]^C BbvCI 1747 GCTGAGG CCTCAGC(-5/-2) BclI 639 TGATCA T^GATCA BsmBI 2315 CGTCTC CGTCTC(1/5) BspLU11I 1922 ACATGT A^CATGT BspMI 1439 GCAGGT ACCTGC(4/8) BsrFI 2770 ACCGGC [GA]^CCGG[CT] BstEII 2145 GGTCACC G^GT[ACGT]ACC BstPI 2145 GGTCACC G^GT[ACGT]ACC Cfr10I 2770 ACCGGC [GA]^CCGG[CT] DraI 2583 TTTAAA TTT^AAA Eco47III 1286 AGCGCT AGC^GCT EcoNI 1077 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2145 GGTCACC G^GT[ACGT]ACC EcoRV 970 GATATC GAT^ATC FbaI 639 TGATCA T^GATCA HaeII 1286 AGCGCT [GA]GCGC^[CT] HapII 2771 CCGG C^CGG HpaI 2563 GTTAAC GTT^AAC HpaII 2771 CCGG C^CGG KpnI 1555 GGTACC GGTAC^C MluNI 470 TGGCCA TGG^CCA MscI 470 TGGCCA TGG^CCA MspI 2771 CCGG C^CGG PacI 31 TTAATTAA TTAAT^TAA PflMI 2799 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1441 AGGTCCC [GA]G^G[AT]CC[CT] SacI 725 GAGCTC GAGCT^C SapI 1060 GAAGAGC GCTCTTC(1/4) SpeI 2851 ACTAGT A^CTAGT SspI 2350 AATATT AAT^ATT SwaI 2582 ATTTAAAT ATTT^AAAT Van91I 2799 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AciI 1437 CCGC CCGC(-3/-1) 7 GCGG AflII 624 CTTAAG C^TTAAG 2811 CTTAAG AflIII 26 ACACGT A^C[GA][CT]GT 1922 ACATGT AvaII 1442 GGTCC G^G[AT]CC 2752 GGACC BbsI 1408 GAAGAC GAAGAC(2/6) 1609 GAAGAC BfrI 624 CTTAAG C^TTAAG 2811 CTTAAG BpuAI 1408 GAAGAC GAAGAC(2/6) 1609 GAAGAC BseRI 2333 CTCCTC GAGGAG(10/8) 1367 GAGGAG BspHI 939 TCATGA T^CATGA 2337 TCATGA BstAPI 820 GCACCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1243 GCAGCAGCTGC Cac8I 411 GCCAGC GC[ACGT]^[ACGT]GC 2493 GCCTGC CfoI 1287 GCGC GCG^C 1698 GCGC DraII 1441 AGGTCCC [GA]G^G[ACGT]CC[CT] 2645 AGGCCCT EaeI 470 TGGCCA [CT]^GGCC[GA] 2772 CGGCCA EcoO109I 1441 AGGTCCC [GA]G^G[ACGT]CC[CT] 2645 AGGCCCT EcoRI 2515 GAATTC G^AATTC 2783 GAATTC EcoT14I 1403 CCAAGG C^C[AT][AT]GG 1446 CCTTGG EcoT22I 308 ATGCAT ATGCA^T 575 ATGCAT HhaI 1287 GCGC GCG^C 1698 GCGC HinP1I 1287 GCGC G^CGC 1698 GCGC HindIII 119 AAGCTT A^AGCTT 2186 AAGCTT MfeI 1460 CAATTG C^AATTG 2152 CAATTG MunI 1460 CAATTG C^AATTG 2152 CAATTG NsiI 308 ATGCAT ATGCA^T 575 ATGCAT NspI 424 GCATGT [GA]CATG^[CT] 1922 ACATGT PstI 667 CTGCAG CTGCA^G 1021 CTGCAG RcaI 939 TCATGA T^CATGA 2337 TCATGA ScaI 628 AGTACT AGT^ACT 948 AGTACT StyI 1403 CCAAGG C^C[AT][AT]GG 1446 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI BbvI BfaI BmyI BpmI BsaBI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII XmnI