(Circular) (Six-base) MAPSORT of: pFN21AB8401.seq Check: 4912 from: 1 to: 7564 pFN21AB8401 7564 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5374 278 Size: 53 83 186 936 3838 2468 Fragments arranged by size: 3838 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4907 1937 Size: 2970 4594 Acc65I G'GTAC_C Cuts at: 3560 4885 3560 Size: 1325 6239 AclI AA'CG_TT Cuts at: 1563 5691 6064 1563 Size: 4128 373 3063 Fragments arranged by size: 4128 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2142 2195 3104 3531 5627 7011 7279 1632 Size: 510 53 909 427 2096 1384 268 1917 Fragments arranged by size: 2096 1917 1384 909 510 427 268 53 AfeI AGC'GCT Cuts at: 3293 3293 Size: 7564 AflII C'TTAA_G Cuts at: 820 1017 2629 4816 820 Size: 197 1612 2187 3568 Fragments arranged by size: 3568 2187 1612 197 AflIII A'CryG_T Cuts at: 1203 2031 3927 6463 7440 1203 Size: 828 1896 2536 977 1327 Fragments arranged by size: 2536 1896 1327 977 828 AgeI A'CCGG_T Cuts at: 6395 6395 Size: 7564 AhdI GACnn_n'nnGTC Cuts at: 6293 6293 Size: 7564 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7564 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4050 1184 Size: 2866 4698 AlwNI CAG_nnn'CTG Cuts at: 2965 3254 3257 4178 4766 5307 6879 2965 Size: 289 3 921 588 541 1572 3650 Fragments arranged by size: 3650 1572 921 588 541 289 3 ApaLI G'TGCA_C Cuts at: 2823 5621 6777 2823 Size: 2798 1156 3610 Fragments arranged by size: 3610 2798 1156 ApoI r'AATT_y Cuts at: 1493 2345 2497 3224 3739 3840 4520 4541 4788 Size: 852 152 727 515 101 680 21 247 Cuts at: 4788 4873 5099 5251 7360 1493 Size: 85 226 152 2109 1697 Fragments arranged by size: 2109 1697 852 727 680 515 247 226 152 152 101 85 21 AseI AT'TA_AT Cuts at: 160 1032 6118 160 Size: 872 5086 1606 Fragments arranged by size: 5086 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7564 AvaI C'yCGr_G Cuts at: 1870 1943 4889 1870 Size: 73 2946 4545 Fragments arranged by size: 4545 2946 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3388 3421 3388 Size: 33 7531 BamHI G'GATC_C Cuts at: 3384 4894 5261 3384 Size: 1510 367 5687 Fragments arranged by size: 5687 1510 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3560 4885 6340 618 Size: 325 217 627 1773 1325 1455 1842 Fragments arranged by size: 1842 1773 1455 1325 627 325 217 BanII G_rGCy'C Cuts at: 1391 2734 4883 1391 Size: 1343 2149 4072 Fragments arranged by size: 4072 2149 1343 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3420 3621 928 Size: 971 1521 201 4871 Fragments arranged by size: 4871 1521 971 201 BbvCI CC'TCA_GC Cuts at: 3753 3753 Size: 7564 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5755 5789 1903 Size: 34 3818 34 3678 Fragments arranged by size: 3818 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5456 6666 5456 Size: 1210 6354 BclI T'GATC_A Cuts at: 1548 2644 1548 Size: 1096 6468 BfrBI ATG'CAT Cuts at: 2315 2582 2315 Size: 267 7297 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 6175 136 Size: 107 122 71 631 605 337 4166 1525 Fragments arranged by size: 4166 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 7559 7559 Size: 7564 BlpI GC'TnA_GC Cuts at: 4981 4981 Size: 7564 Bme1580I G_kGCm'C Cuts at: 1790 2827 3583 5625 6781 1790 Size: 1037 756 2042 1156 2573 Fragments arranged by size: 2573 2042 1156 1037 756 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7564 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5295 6253 442 Size: 416 53 83 399 24 3878 958 1753 Fragments arranged by size: 3878 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7564 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2256 4449 4626 6224 1239 Size: 1017 2193 177 1598 2579 Fragments arranged by size: 2579 2193 1598 1017 177 Bpu10I CC'TnA_GC Cuts at: 3584 3753 3584 Size: 169 7395 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3649 3839 5689 6554 6852 3649 Size: 190 1850 865 298 4361 Fragments arranged by size: 4361 1850 865 298 190 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6227 882 Size: 617 4728 2219 Fragments arranged by size: 4728 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5355 493 Size: 711 4151 2702 Fragments arranged by size: 4151 2702 711 BsaBI GATnn'nnATC Cuts at: 2186 2610 3834 7558 2186 Size: 424 1224 3724 2192 Fragments arranged by size: 3724 2192 1224 424 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5371 5753 6388 275 Size: 53 83 186 936 3838 382 635 1451 Fragments arranged by size: 3838 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5997 6395 6669 6816 7227 5997 Size: 398 274 147 411 6334 Fragments arranged by size: 6334 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4321 4351 1178 Size: 30 165 30 2918 30 4391 Fragments arranged by size: 4391 2918 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3387 4329 1186 Size: 2201 942 4421 Fragments arranged by size: 4421 2201 942 BseYI C'CCAG_C Cuts at: 2798 6767 7263 2798 Size: 3969 496 3099 Fragments arranged by size: 3969 3099 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6991 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5014 5775 6803 1540 Size: 275 186 3013 761 1028 2301 Fragments arranged by size: 3013 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2734 2827 2996 5625 5710 6781 2734 Size: 93 169 2629 85 1071 3517 Fragments arranged by size: 3517 2629 1071 169 93 85 BsmI GAATG_Cn' Cuts at: 2584 3166 3315 3911 5075 5168 2584 Size: 582 149 596 1164 93 4980 Fragments arranged by size: 4980 1164 596 582 149 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4326 4326 Size: 7564 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2734 2827 2996 3583 4883 5625 5710 Size: 399 944 93 169 587 1300 742 85 Cuts at: 5710 6781 1391 Size: 1071 2174 Fragments arranged by size: 2174 1300 1071 944 742 587 399 169 93 85 BspEI T'CCGG_A Cuts at: 7227 7227 Size: 7564 BspHI T'CATG_A Cuts at: 2944 4342 5453 7183 2944 Size: 1398 1111 1730 3325 Fragments arranged by size: 3325 1730 1398 1111 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3435 4919 844 Size: 2591 1484 3489 Fragments arranged by size: 3489 2591 1484 BsrBI CCG'CTC Cuts at: 1394 1823 5451 1394 Size: 429 3628 3507 Fragments arranged by size: 3628 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3161 3698 4098 6059 6233 65 Size: 1183 1913 537 400 1961 174 1396 Fragments arranged by size: 1961 1913 1396 1183 537 400 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4775 6208 6395 1700 Size: 110 2965 1433 187 2869 Fragments arranged by size: 2965 2869 1433 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7564 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7564 BssSI C'ACGA_G Cuts at: 5624 6636 5624 Size: 1012 6552 BstAPI GCAn_nnn'nTGC Cuts at: 794 2831 3254 794 Size: 2037 423 5104 Fragments arranged by size: 5104 2037 423 BstBI TT'CG_AA Cuts at: 6368 6368 Size: 7564 BstEII G'GTnAC_C Cuts at: 1184 4150 1184 Size: 2966 4598 BstYI r'GATC_y Cuts at: 1972 2326 2357 2962 3040 3384 3438 3459 4123 Size: 354 31 605 78 344 54 21 664 Cuts at: 4123 4174 4297 4894 5261 5646 5663 7115 7201 Size: 51 123 597 367 385 17 1452 86 Cuts at: 7201 7213 7559 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 664 605 597 385 367 354 346 344 123 86 78 54 51 31 21 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7012 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6825 Fragments arranged by size: 6825 683 56 BtsI GCAGTG_nn' Cuts at: 5080 5874 5894 5080 Size: 794 20 6750 Fragments arranged by size: 6750 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7564 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7529 DraI TTT'AAA Cuts at: 4590 4868 4590 Size: 278 7286 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6571 809 Size: 507 5255 1802 Fragments arranged by size: 5255 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2475 4777 5011 Size: 54 1425 50 275 18 645 2302 234 Cuts at: 5011 5900 7529 8 Size: 889 1629 43 Fragments arranged by size: 2302 1629 1425 889 645 275 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5011 1537 Size: 275 3199 4090 Fragments arranged by size: 4090 3199 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2214 2370 3055 3060 3807 3945 5494 1353 Size: 861 156 685 5 747 138 1549 3423 Fragments arranged by size: 3423 1549 861 747 685 156 138 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6151 6525 6671 387 Size: 1064 138 4562 374 146 1280 Fragments arranged by size: 4562 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2732 2732 Size: 7564 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2142 2195 2256 3104 3531 4449 4626 Size: 393 510 53 61 848 427 918 177 Cuts at: 4626 5627 6224 7011 7279 1239 Size: 1001 597 787 268 1524 Fragments arranged by size: 1524 1001 918 848 787 597 510 427 393 268 177 61 53 EcoNI CCTnn'n_nnAGG Cuts at: 3086 3086 Size: 7564 EcoO109I rG'GnC_Cy Cuts at: 3447 4651 3447 Size: 1204 6360 EcoRI G'AATT_C Cuts at: 4520 4788 4873 5251 4520 Size: 268 85 378 6833 Fragments arranged by size: 6833 378 268 85 EcoRV GAT'ATC Cuts at: 2977 2977 Size: 7564 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4736 4736 Size: 7564 FspI TGC'GCA Cuts at: 6070 6070 Size: 7564 HaeII r_GCGC'y Cuts at: 1678 3295 6711 1678 Size: 1617 3416 2531 Fragments arranged by size: 3416 2531 1617 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2096 2128 3032 3064 3944 Size: 32 135 32 524 32 904 32 880 Cuts at: 3944 3976 4321 4353 4818 4850 6205 6237 6279 Size: 32 345 32 465 32 1355 32 42 Cuts at: 6279 6311 1373 Size: 32 2626 Fragments arranged by size: 2626 1355 904 880 524 465 345 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4207 4570 4620 4908 5154 669 Size: 988 281 2269 363 50 288 246 3079 Fragments arranged by size: 3079 2269 988 363 288 281 246 50 HindIII A'AGCT_T Cuts at: 748 2124 4191 748 Size: 1376 2067 4121 Fragments arranged by size: 4121 2067 1376 HpaI GTT'AAC Cuts at: 4570 5154 4570 Size: 584 6980 KpnI G_GTAC'C Cuts at: 3564 4889 3564 Size: 1325 6239 MfeI C'AATT_G Cuts at: 3465 4157 5163 3465 Size: 692 1006 5866 Fragments arranged by size: 5866 1006 692 MluI A'CGCG_T Cuts at: 7440 7440 Size: 7564 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6678 6862 1305 Size: 5373 184 2007 Fragments arranged by size: 5373 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2477 10 Size: 54 1425 343 645 5097 Fragments arranged by size: 5097 1425 645 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2340 2451 4577 5522 5881 6040 518 Size: 648 1174 111 2126 945 359 159 2042 Fragments arranged by size: 2126 2042 1174 945 648 359 159 111 MspA1I CmG'CkG Cuts at: 1606 2677 3254 4075 4980 5657 6805 7050 1606 Size: 1071 577 821 905 677 1148 245 2120 Fragments arranged by size: 2120 1148 1071 905 821 677 577 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7012 NdeI CA'TA_TG Cuts at: 387 387 Size: 7564 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7564 NotI GC'GGCC_GC Cuts at: 5011 5011 Size: 7564 NsiI A_TGCA'T Cuts at: 2317 2584 2317 Size: 267 7297 NspI r_CATG'y Cuts at: 1132 1229 2433 3931 4922 6467 1132 Size: 97 1204 1498 991 1545 2229 Fragments arranged by size: 2229 1545 1498 1204 991 97 PacI TTA_AT'TAA Cuts at: 2040 2040 Size: 7564 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7564 PciI A'CATG_T Cuts at: 3927 6463 3927 Size: 2536 5028 PflMI CCAn_nnn'nTGG Cuts at: 1108 4810 1108 Size: 3702 3862 PfoI T'CCnGG_A Cuts at: 2170 4224 2170 Size: 2054 5510 PmeI GTTT'AAAC Cuts at: 4868 4868 Size: 7564 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5633 5665 7146 7178 5633 Size: 32 1481 32 6019 Fragments arranged by size: 6019 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3447 3447 Size: 7564 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7564 PsiI TTA'TAA Cuts at: 2700 5134 2700 Size: 2434 5130 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2012 2044 2496 2528 2940 2972 3519 3551 3702 Size: 32 452 32 412 32 547 32 151 Cuts at: 3702 3734 2012 Size: 32 5842 Fragments arranged by size: 5842 547 452 412 151 32 32 32 32 32 PstI C_TGCA'G Cuts at: 830 2676 3030 4916 830 Size: 1846 354 1886 3478 Fragments arranged by size: 3478 1886 1846 354 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7564 PvuII CAG'CTG Cuts at: 2677 3254 4075 2677 Size: 577 821 6166 Fragments arranged by size: 6166 821 577 SacI G_AGCT'C Cuts at: 2734 2734 Size: 7564 SalI G'TCGA_C Cuts at: 1936 4906 1936 Size: 2970 4594 SapI GCTCTTCn'nnn_ Cuts at: 3060 3060 Size: 7564 SbfI CC_TGCA'GG Cuts at: 4916 4916 Size: 7564 ScaI AGT'ACT Cuts at: 2635 2955 5812 7524 2635 Size: 320 2857 1712 2675 Fragments arranged by size: 2857 2675 1712 320 SfcI C'TryA_G Cuts at: 826 1045 2491 2672 2957 3026 3400 3525 4912 Size: 219 1446 181 285 69 374 125 1387 Cuts at: 4912 6047 6728 6919 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1446 1387 1135 681 374 285 219 191 181 125 69 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7564 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7564 SmaI CCC'GGG Cuts at: 1872 4891 1872 Size: 3019 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2629 3628 3854 4816 5668 6569 Size: 197 926 686 999 226 962 852 901 Cuts at: 6569 6831 820 Size: 262 1553 Fragments arranged by size: 1553 999 962 926 901 852 686 262 226 197 SnaBI TAC'GTA Cuts at: 493 5355 493 Size: 4862 2702 SpeI A'CTAG_T Cuts at: 152 4856 152 Size: 4704 2860 SphI G_CATG'C Cuts at: 1132 4922 1132 Size: 3790 3774 SspI AAT'ATT Cuts at: 5 52 4357 5488 5 Size: 47 4305 1131 2081 Fragments arranged by size: 4305 2081 1131 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7564 StyI C'CwwG_G Cuts at: 513 1065 3408 3451 5003 513 Size: 552 2343 43 1552 3074 Fragments arranged by size: 3074 2343 1552 552 43 SwaI ATTT'AAAT Cuts at: 4590 4590 Size: 7564 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4169 5591 5608 5761 5946 6391 325 Size: 1255 2589 1422 17 153 185 445 1498 Fragments arranged by size: 2589 1498 1422 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2633 2953 3997 Size: 274 80 33 51 1443 656 320 1044 Cuts at: 3997 4231 5810 7522 96 Size: 234 1579 1712 138 Fragments arranged by size: 1712 1579 1443 1044 656 320 274 234 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7524 7556 7524 Size: 32 7532 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7564 XbaI T'CTAG_A Cuts at: 2092 3053 3593 4900 2092 Size: 961 540 1307 4756 Fragments arranged by size: 4756 1307 961 540 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7564 XmaI C'CCGG_G Cuts at: 1870 4889 1870 Size: 3019 4545 XmnI GAAnn'nnTTC Cuts at: 2166 2256 2349 3285 3339 3417 5693 2166 Size: 90 93 936 54 78 2276 4037 Fragments arranged by size: 4037 2276 936 93 90 78 54 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5372 276 Size: 53 83 186 936 3838 2468 Fragments arranged by size: 3838 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BplI BsiWI BstXI BstZ17I Bsu36I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI PmlI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI XcmI