(Linear) (Six-base) MAPSORT of: KIBB8401.seq Check: 609 from: 1 to: 2859 KIBB8401.orf With 182 enzymes: SgfI * July 15, 2010 18:31 .. Acc65I G'GTAC_C Cuts at: 0 1555 2859 Size: 1555 1304 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 137 190 1099 1526 2859 Size: 137 53 909 427 1333 Fragments arranged by size: 1333 909 427 137 53 AfeI AGC'GCT Cuts at: 0 1288 2859 Size: 1288 1571 AflII C'TTAA_G Cuts at: 0 624 2811 2859 Size: 624 2187 48 Fragments arranged by size: 2187 624 48 AflIII A'CryG_T Cuts at: 0 26 1922 2859 Size: 26 1896 937 Fragments arranged by size: 1896 937 26 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2045 2859 Size: 2045 814 AlwNI CAG_nnn'CTG Cuts at: 0 960 1249 1252 2173 2761 2859 Size: 960 289 3 921 588 98 Fragments arranged by size: 960 921 588 289 98 3 ApaLI G'TGCA_C Cuts at: 0 818 2859 Size: 818 2041 ApoI r'AATT_y Cuts at: 0 340 492 1219 1734 1835 2515 2536 2783 Size: 340 152 727 515 101 680 21 247 Cuts at: 2783 2859 Size: 76 Fragments arranged by size: 727 680 515 340 247 152 101 76 21 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1383 1416 2859 Size: 1383 33 1443 Fragments arranged by size: 1443 1383 33 BamHI G'GATC_C Cuts at: 0 1379 2859 Size: 1379 1480 BanI G'GyrC_C Cuts at: 0 1555 2859 Size: 1555 1304 BanII G_rGCy'C Cuts at: 0 729 2859 Size: 729 2130 BbsI GAAGACnn'nnnn_ Cuts at: 0 1415 1616 2859 Size: 1415 201 1243 Fragments arranged by size: 1415 1243 201 BbvCI CC'TCA_GC Cuts at: 0 1748 2859 Size: 1748 1111 BclI T'GATC_A Cuts at: 0 639 2859 Size: 639 2220 BfrBI ATG'CAT Cuts at: 0 310 577 2859 Size: 310 267 2282 Fragments arranged by size: 2282 310 267 Bme1580I G_kGCm'C Cuts at: 0 822 1578 2859 Size: 822 756 1281 Fragments arranged by size: 1281 822 756 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 251 2444 2621 2859 Size: 251 2193 177 238 Fragments arranged by size: 2193 251 238 177 Bpu10I CC'TnA_GC Cuts at: 0 1579 1748 2859 Size: 1579 169 1111 Fragments arranged by size: 1579 1111 169 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1644 1834 2859 Size: 1644 190 1025 Fragments arranged by size: 1644 1025 190 BsaBI GATnn'nnATC Cuts at: 0 181 605 1829 2859 Size: 181 424 1224 1030 Fragments arranged by size: 1224 1030 424 181 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2316 2346 2859 Size: 2316 30 513 Fragments arranged by size: 2316 513 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1382 2324 2859 Size: 1382 942 535 Fragments arranged by size: 1382 942 535 BseYI C'CCAG_C Cuts at: 0 793 2859 Size: 793 2066 BsiHKAI G_wGCw'C Cuts at: 0 729 822 991 2859 Size: 729 93 169 1868 Fragments arranged by size: 1868 729 169 93 BsmI GAATG_Cn' Cuts at: 0 579 1161 1310 1906 2859 Size: 579 582 149 596 953 Fragments arranged by size: 953 596 582 579 149 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2321 2859 Size: 2321 538 Bsp1286I G_dGCh'C Cuts at: 0 729 822 991 1578 2859 Size: 729 93 169 587 1281 Fragments arranged by size: 1281 729 587 169 93 BspHI T'CATG_A Cuts at: 0 939 2337 2859 Size: 939 1398 522 Fragments arranged by size: 1398 939 522 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1430 2859 Size: 1430 1429 BsrDI GCAATG_nn' Cuts at: 0 1156 1693 2093 2859 Size: 1156 537 400 766 Fragments arranged by size: 1156 766 537 400 BsrFI r'CCGG_y Cuts at: 0 2770 2859 Size: 2770 89 BstAPI GCAn_nnn'nTGC Cuts at: 0 826 1249 2859 Size: 826 423 1610 Fragments arranged by size: 1610 826 423 BstEII G'GTnAC_C Cuts at: 0 2145 2859 Size: 2145 714 BstYI r'GATC_y Cuts at: 0 321 352 957 1035 1379 1433 1454 2118 Size: 321 31 605 78 344 54 21 664 Cuts at: 2118 2169 2292 2859 Size: 51 123 567 Fragments arranged by size: 664 605 567 344 321 123 78 54 51 31 21 DraI TTT'AAA Cuts at: 0 2585 2859 Size: 2585 274 EaeI y'GGCC_r Cuts at: 0 470 2772 2859 Size: 470 2302 87 Fragments arranged by size: 2302 470 87 EarI CTCTTCn'nnn_ Cuts at: 0 209 365 1050 1055 1802 1940 2859 Size: 209 156 685 5 747 138 919 Fragments arranged by size: 919 747 685 209 156 138 5 EcoICRI GAG'CTC Cuts at: 0 727 2859 Size: 727 2132 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 137 190 251 1099 1526 2444 2621 2859 Size: 137 53 61 848 427 918 177 238 Fragments arranged by size: 918 848 427 238 177 137 61 53 EcoNI CCTnn'n_nnAGG Cuts at: 0 1081 2859 Size: 1081 1778 EcoO109I rG'GnC_Cy Cuts at: 0 1442 2646 2859 Size: 1442 1204 213 Fragments arranged by size: 1442 1204 213 EcoRI G'AATT_C Cuts at: 0 2515 2783 2859 Size: 2515 268 76 Fragments arranged by size: 2515 268 76 EcoRV GAT'ATC Cuts at: 0 972 2859 Size: 972 1887 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2731 2859 Size: 2731 128 HaeII r_GCGC'y Cuts at: 0 1290 2859 Size: 1290 1569 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 91 123 1027 1059 1939 1971 2316 2348 Size: 91 32 904 32 880 32 345 32 Cuts at: 2348 2813 2845 2859 Size: 465 32 14 Fragments arranged by size: 904 880 465 345 91 32 32 32 32 32 14 HincII GTy'rAC Cuts at: 0 2202 2565 2615 2859 Size: 2202 363 50 244 Fragments arranged by size: 2202 363 244 50 HindIII A'AGCT_T Cuts at: 0 119 2186 2859 Size: 119 2067 673 Fragments arranged by size: 2067 673 119 HpaI GTT'AAC Cuts at: 0 2565 2859 Size: 2565 294 KpnI G_GTAC'C Cuts at: 0 1559 2859 Size: 1559 1300 MfeI C'AATT_G Cuts at: 0 1460 2152 2859 Size: 1460 692 707 Fragments arranged by size: 1460 707 692 MscI TGG'CCA Cuts at: 0 472 2859 Size: 472 2387 MslI CAynn'nnrTG Cuts at: 0 335 446 2572 2859 Size: 335 111 2126 287 Fragments arranged by size: 2126 335 287 111 MspA1I CmG'CkG Cuts at: 0 672 1249 2070 2859 Size: 672 577 821 789 Fragments arranged by size: 821 789 672 577 NsiI A_TGCA'T Cuts at: 0 312 579 2859 Size: 312 267 2280 Fragments arranged by size: 2280 312 267 NspI r_CATG'y Cuts at: 0 428 1926 2859 Size: 428 1498 933 Fragments arranged by size: 1498 933 428 PacI TTA_AT'TAA Cuts at: 0 35 2859 Size: 35 2824 PciI A'CATG_T Cuts at: 0 1922 2859 Size: 1922 937 PflMI CCAn_nnn'nTGG Cuts at: 0 2805 2859 Size: 2805 54 PfoI T'CCnGG_A Cuts at: 0 165 2219 2859 Size: 165 2054 640 Fragments arranged by size: 2054 640 165 PpuMI rG'GwC_Cy Cuts at: 0 1442 2859 Size: 1442 1417 PsiI TTA'TAA Cuts at: 0 695 2859 Size: 695 2164 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 7 39 491 523 935 967 1514 1546 Size: 7 32 452 32 412 32 547 32 Cuts at: 1546 1697 1729 2859 Size: 151 32 1130 Fragments arranged by size: 1130 547 452 412 151 32 32 32 32 32 7 PstI C_TGCA'G Cuts at: 0 671 1025 2859 Size: 671 354 1834 Fragments arranged by size: 1834 671 354 PvuII CAG'CTG Cuts at: 0 672 1249 2070 2859 Size: 672 577 821 789 Fragments arranged by size: 821 789 672 577 SacI G_AGCT'C Cuts at: 0 729 2859 Size: 729 2130 SapI GCTCTTCn'nnn_ Cuts at: 0 1055 2859 Size: 1055 1804 ScaI AGT'ACT Cuts at: 0 630 950 2859 Size: 630 320 1909 Fragments arranged by size: 1909 630 320 SfcI C'TryA_G Cuts at: 0 486 667 952 1021 1395 1520 2859 Size: 486 181 285 69 374 125 1339 Fragments arranged by size: 1339 486 374 285 181 125 69 SmlI C'TyrA_G Cuts at: 0 624 1623 1849 2811 2859 Size: 624 999 226 962 48 Fragments arranged by size: 999 962 624 226 48 SpeI A'CTAG_T Cuts at: 0 2851 2859 Size: 2851 8 SspI AAT'ATT Cuts at: 0 2352 2859 Size: 2352 507 StyI C'CwwG_G Cuts at: 0 1403 1446 2859 Size: 1403 43 1413 Fragments arranged by size: 1413 1403 43 SwaI ATTT'AAAT Cuts at: 0 2585 2859 Size: 2585 274 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2164 2859 Size: 2164 695 TatI w'GTAC_w Cuts at: 0 628 948 1992 2226 2859 Size: 628 320 1044 234 633 Fragments arranged by size: 1044 633 628 320 234 XbaI T'CTAG_A Cuts at: 0 87 1048 1588 2859 Size: 87 961 540 1271 Fragments arranged by size: 1271 961 540 87 XmnI GAAnn'nnTTC Cuts at: 0 161 251 344 1280 1334 1412 2859 Size: 161 90 93 936 54 78 1447 Fragments arranged by size: 1447 936 161 93 90 78 54 Enzymes that do cut: Acc65I AcuI AfeI AflII AflIII AlfI AlwNI ApaLI ApoI BaeI BamHI BanI BanII BbsI BbvCI BclI BfrBI Bme1580I BpmI Bpu10I BpuEI BsaBI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstEII BstYI DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MscI MslI MspA1I NsiI NspI PacI PciI PflMI PfoI PpuMI PsiI PsrI PstI PvuII SacI SapI ScaI SfcI SmlI SpeI SspI StyI SwaI TaqII TatI XbaI XmnI Enzymes that do not cut: AarI AatII AccI AclI AgeI AhdI AleI AloI ApaI AscI AseI AsiSI AvaI AvrII BbeI BcgI BciVI BglI BglII BlpI BmgBI BmrI BmtI BplI BsaI BsaAI BsaHI BsaWI BsgI BsiEI BsiWI BspEI BsrBI BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EagI EciI FseI FspI FspAI KasI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PasI PmeI PmlI PpiI PshAI PspOMI PspXI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI StuI TstI Tth111I XcmI XhoI XmaI ZraI