################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BfrI BglI BglII BlnI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HincII HindII HpaI HphI KpnI KspI MamI MfeI MflI MluI MroI MslI MunI NcoI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfuI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 121 GACGTC GACGT^C AfaI 63 GTAC GT^AC AlwI 378 GATCC GGATC(4/5) ApaI 587 GGGCCC GGGCC^C Asp700I 552 GAATCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 455 GTGCTC G[AT]GC[AT]^C BalI 320 TGGCCA TGG^CCA BbeI 153 GGCGCC GGCGC^C BbsI 788 GTCTTC GAAGAC(2/6) BciVI 187 GGATAC GTATCC(6/5) BclI 737 TGATCA T^GATCA BlpI 466 GCTGAGC GC^T[ACGT]AGC BpmI 140 CTGGAG CTGGAG(16/14) Bpu1102I 466 GCTGAGC GC^T[ACGT]AGC BpuAI 788 GTCTTC GAAGAC(2/6) BsaI 143 GAGACC GGTCTC(1/5) BsiHKAI 455 GTGCTC G[AT]GC[AT]^C BsmAI 143 GAGAC GTCTC(1/5) BsmI 753 GAATGC GAATGC(1/-1) BspHI 858 TCATGA T^CATGA BsrBI 67 GAGCGG CCGCTC(-3/-3) BsrFI 605 GCCGGC [GA]^CCGG[CT] CelII 466 GCTGAGC GC^T[ACGT]AGC Cfr10I 605 GCCGGC [GA]^CCGG[CT] DraIII 634 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 779 GACAGGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 636 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 758 CCAAGG C^C[AT][AT]GG FbaI 737 TGATCA T^GATCA HaeII 153 GGCGCC [GA]GCGC^[CT] HgaI 43 GACGC GACGC(5/10) HindIII 652 AAGCTT A^AGCTT KasI 153 GGCGCC G^GCGCC MaeII 122 ACGT A^CGT MaeIII 896 GTTAC ^GT[ACGT]AC MluNI 320 TGGCCA TGG^CCA MscI 320 TGGCCA TGG^CCA MspA1I 464 CAGCTG C[AC]G^C[GT]G NaeI 605 GCCGGC GCC^GGC NarI 153 GGCGCC GG^CGCC NgoMIV 605 GCCGGC G^CCGGC NheI 889 GCTAGC G^CTAGC PpuMI 497 AGGTCCT [GA]G^G[AT]CC[CT] PvuII 464 CAGCTG CAG^CTG RcaI 858 TCATGA T^CATGA RsaI 63 GTAC GT^AC SapI 625 GAAGAGC GCTCTTC(1/4) SexAI 635 ACCTGGT A^CC[AT]GGT SfcI 965 CTACAG C^T[GA][CT]AG SfoI 153 GGCGCC GGC^GCC SgrAI 604 CGCCGGCG C[GA]^CCGG[CT]G SmaI 815 CCCGGG CCC^GGG SphI 302 GCATGC GCATG^C StyI 758 CCAAGG C^C[AT][AT]GG XmaCI 815 CCCGGG C^CCGGG XmaI 815 CCCGGG C^CCGGG XmnI 552 GAATCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 12 CGCG CG^CG 945 CGCG AlwNI 55 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG 631 CAGCACCTG AvaII 102 GGACC G^G[AT]CC 498 GGTCC BanI 153 GGCGCC G^G[CT][GA]CC 932 GGCACC BanII 587 GGGCCC G[GA]GC[CT]^C 833 GAGCCC BseRI 239 CTCCTC GAGGAG(10/8) 311 GAGGAG BsrI 885 ACTGG ACTGG(1/-1) 54 CCAGT BstF5I 126 CATCC GGATG(2/0) 83 GGATG BstUI 12 CGCG CG^CG 945 CGCG EaeI 51 CGGCCA [CT]^GGCC[GA] 320 TGGCCA EarI 766 CTCTTC CTCTTC(1/4) 625 GAAGAG FokI 126 CATCC GGATG(9/13) 83 GGATG Ksp632I 766 CTCTTC CTCTTC(1/4) 625 GAAGAG MseI 16 TTAA T^TAA 73 TTAA MvnI 12 CGCG CG^CG 945 CGCG NspI 302 GCATGC [GA]CATG^[CT] 938 GCATGT PleI 26 GAGTC GAGTC(4/5) 439 GAGTC TaqI 215 TCGA T^CGA 831 TCGA TfiI 217 GATTC G^A[AT]TC 552 GAATC Tru9I 16 TTAA T^TAA 73 TTAA Tsp509I 223 AATT ^AATT 482 AATT TspRI 272 CACTG CA[GC]TG[ACGT][ACGT]^ 393 CACTG TthHB8I 215 TCGA T^CGA 831 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AcyI AluI AvaI BbvI BcnI BfaI BmyI BsaHI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI MaeI MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI