(Circular) (Six-base) MAPSORT of: pFN21AE6347.seq Check: 4868 from: 1 to: 5686 pFN21AE6347 5686 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2130 3496 278 Size: 53 83 186 936 594 1366 2468 Fragments arranged by size: 2468 1366 936 594 186 83 53 AccI GT'mk_AC Cuts at: 1937 3029 1937 Size: 1092 4594 Acc65I G'GTAC_C Cuts at: 3007 3007 Size: 5686 AclI AA'CG_TT Cuts at: 1563 3813 4186 1563 Size: 2250 373 3063 Fragments arranged by size: 3063 2250 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2141 2480 2649 2714 2758 2903 3749 5133 Size: 509 339 169 65 44 145 846 1384 Cuts at: 5133 5401 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 846 509 339 268 169 145 65 44 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5489 AflIII A'CryG_T Cuts at: 1203 4585 5562 1203 Size: 3382 977 1327 Fragments arranged by size: 3382 1327 977 AgeI A'CCGG_T Cuts at: 4517 4517 Size: 5686 AhdI GACnn_n'nnGTC Cuts at: 4415 4415 Size: 5686 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5686 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5686 AlwNI CAG_nnn'CTG Cuts at: 2065 2641 3429 5001 2065 Size: 576 788 1572 2750 Fragments arranged by size: 2750 1572 788 576 ApaI G_GGCC'C Cuts at: 2596 2596 Size: 5686 ApaLI G'TGCA_C Cuts at: 3743 4899 3743 Size: 1156 4530 ApoI r'AATT_y Cuts at: 1493 2995 3221 3373 5482 1493 Size: 1502 226 152 2109 1697 Fragments arranged by size: 2109 1697 1502 226 152 AseI AT'TA_AT Cuts at: 160 1032 4240 160 Size: 872 3208 1606 Fragments arranged by size: 3208 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 AvaI C'yCGr_G Cuts at: 1870 1943 2101 2188 2820 3011 1870 Size: 73 158 87 632 191 4545 Fragments arranged by size: 4545 632 191 158 87 73 BamHI G'GATC_C Cuts at: 3016 3383 3016 Size: 367 5319 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2158 2937 3007 4462 618 Size: 325 217 627 371 779 70 1455 1842 Fragments arranged by size: 1842 1455 779 627 371 325 217 70 BanII G_rGCy'C Cuts at: 1391 2596 2842 3005 1391 Size: 1205 246 163 4072 Fragments arranged by size: 4072 1205 246 163 BbeI G_GCGC'C Cuts at: 2162 2162 Size: 5686 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2786 928 Size: 971 887 3828 Fragments arranged by size: 3828 971 887 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3877 3911 1903 Size: 34 1940 34 3678 Fragments arranged by size: 3678 1940 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2186 3578 4788 2186 Size: 1392 1210 3084 Fragments arranged by size: 3084 1392 1210 BclI T'GATC_A Cuts at: 1548 2742 1548 Size: 1194 4492 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4297 136 Size: 107 122 71 631 605 2625 1525 Fragments arranged by size: 2625 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5681 5681 Size: 5686 BlpI GC'TnA_GC Cuts at: 2472 3103 2472 Size: 631 5055 Bme1580I G_kGCm'C Cuts at: 1790 2596 3747 4903 1790 Size: 806 1151 1156 2573 Fragments arranged by size: 2573 1156 1151 806 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5686 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3417 4375 442 Size: 416 53 83 399 24 2000 958 1753 Fragments arranged by size: 2000 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2898 1056 Size: 1842 3844 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2166 4346 1239 Size: 927 2180 2579 Fragments arranged by size: 2579 2180 927 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3811 4676 4974 3811 Size: 865 298 4523 Fragments arranged by size: 4523 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2142 4349 882 Size: 617 643 2207 2219 Fragments arranged by size: 2219 2207 643 617 BsaAI yAC'GTr Cuts at: 493 1204 3477 493 Size: 711 2273 2702 Fragments arranged by size: 2702 2273 711 BsaBI GATnn'nnATC Cuts at: 5680 5680 Size: 5686 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2049 2127 2159 3493 Size: 53 83 186 936 516 78 32 1334 Cuts at: 3493 3875 4510 275 Size: 382 635 1451 Fragments arranged by size: 1451 1334 936 635 516 382 186 83 78 53 32 BsaWI w'CCGG_w Cuts at: 4119 4517 4791 4938 5349 4119 Size: 398 274 147 411 4456 Fragments arranged by size: 4456 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2139 2169 2970 3000 1178 Size: 30 165 30 736 30 801 30 3864 Fragments arranged by size: 3864 801 736 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2235 2331 1186 Size: 1049 96 4541 Fragments arranged by size: 4541 1049 96 BseYI C'CCAG_C Cuts at: 2293 2365 4889 5385 2293 Size: 72 2524 496 2594 Fragments arranged by size: 2594 2524 496 72 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5113 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3136 3897 4925 1540 Size: 275 186 1135 761 1028 2301 Fragments arranged by size: 2301 1135 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2464 3747 3832 4903 2464 Size: 1283 85 1071 3247 Fragments arranged by size: 3247 1283 1071 85 BsmI GAATG_Cn' Cuts at: 2764 3197 3290 2764 Size: 433 93 5160 Fragments arranged by size: 5160 433 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2464 2596 2842 3005 3747 3832 4903 Size: 399 674 132 246 163 742 85 1071 Cuts at: 4903 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 674 399 246 163 132 85 BspEI T'CCGG_A Cuts at: 5349 5349 Size: 5686 BspHI T'CATG_A Cuts at: 2863 3575 5305 2863 Size: 712 1730 3244 Fragments arranged by size: 3244 1730 712 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3041 844 Size: 2197 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2074 3573 1394 Size: 429 251 1499 3507 Fragments arranged by size: 3507 1499 429 251 BsrDI GCAATG_nn' Cuts at: 65 1248 4181 4355 65 Size: 1183 2933 174 1396 Fragments arranged by size: 2933 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2610 4330 4517 1700 Size: 110 800 1720 187 2869 Fragments arranged by size: 2869 1720 800 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5686 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5686 BssSI C'ACGA_G Cuts at: 3746 4758 3746 Size: 1012 4674 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5686 BstBI TT'CG_AA Cuts at: 4490 4490 Size: 5686 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5686 BstYI r'GATC_y Cuts at: 1972 3016 3383 3768 3785 5237 5323 5335 5681 Size: 1044 367 385 17 1452 86 12 346 Cuts at: 5681 1972 Size: 1977 Fragments arranged by size: 1977 1452 1044 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4195 Fragments arranged by size: 4195 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2976 487 Size: 683 56 1750 3197 Fragments arranged by size: 3197 1750 683 56 BtsI GCAGTG_nn' Cuts at: 3202 3996 4016 3202 Size: 794 20 4872 Fragments arranged by size: 4872 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5686 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5651 DraI TTT'AAA Cuts at: 2990 2990 Size: 5686 DraIII CAC_nnn'GTG Cuts at: 2644 2644 Size: 5686 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2790 4693 809 Size: 507 1474 1903 1802 Fragments arranged by size: 1903 1802 1474 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2056 2325 3133 Size: 54 1425 50 275 18 226 269 808 Cuts at: 3133 4022 5651 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 808 275 269 226 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3133 1537 Size: 275 1321 4090 Fragments arranged by size: 4090 1321 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2625 2777 3616 1353 Size: 1272 152 839 3423 Fragments arranged by size: 3423 1272 839 152 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2540 4273 4647 4793 387 Size: 1064 138 951 1733 374 146 1280 Fragments arranged by size: 1733 1280 1064 951 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2141 2166 2480 2649 2714 2758 2903 Size: 393 509 25 314 169 65 44 145 Cuts at: 2903 3749 4346 5133 5401 1239 Size: 846 597 787 268 1524 Fragments arranged by size: 1524 846 787 597 509 393 314 268 169 145 65 44 25 EcoNI CCTnn'n_nnAGG Cuts at: 2645 2645 Size: 5686 EcoO109I rG'GnC_Cy Cuts at: 2503 2592 2593 2767 2503 Size: 89 1 174 5422 Fragments arranged by size: 5422 174 89 1 EcoRI G'AATT_C Cuts at: 2995 3373 2995 Size: 378 5308 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2788 2788 Size: 5686 FspI TGC'GCA Cuts at: 4192 4192 Size: 5686 HaeII r_GCGC'y Cuts at: 1678 2162 4833 1678 Size: 484 2671 2531 Fragments arranged by size: 2671 2531 484 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2794 2826 4327 4359 4401 Size: 32 135 32 1222 32 1501 32 42 Cuts at: 4401 4433 1373 Size: 32 2626 Fragments arranged by size: 2626 1501 1222 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3030 3276 669 Size: 988 281 1092 246 3079 Fragments arranged by size: 3079 1092 988 281 246 HindIII A'AGCT_T Cuts at: 748 2657 748 Size: 1909 3777 HpaI GTT'AAC Cuts at: 3276 3276 Size: 5686 KasI G'GCGC_C Cuts at: 2158 2158 Size: 5686 KpnI G_GTAC'C Cuts at: 3011 3011 Size: 5686 MfeI C'AATT_G Cuts at: 3285 3285 Size: 5686 MluI A'CGCG_T Cuts at: 5562 5562 Size: 5686 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4800 4984 1305 Size: 3495 184 2007 Fragments arranged by size: 3495 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2327 10 Size: 54 1425 343 495 3369 Fragments arranged by size: 3369 1425 495 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3644 4003 4162 518 Size: 648 2478 359 159 2042 Fragments arranged by size: 2478 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2471 3102 3779 4927 5172 1606 Size: 865 631 677 1148 245 2120 Fragments arranged by size: 2120 1148 865 677 631 245 NaeI GCC'GGC Cuts at: 2612 2612 Size: 5686 NarI GG'CG_CC Cuts at: 2159 2159 Size: 5686 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4195 Fragments arranged by size: 4195 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5686 NgoMIV G'CCGG_C Cuts at: 2610 2610 Size: 5686 NheI G'CTAG_C Cuts at: 1052 2894 1052 Size: 1842 3844 NotI GC'GGCC_GC Cuts at: 3133 3133 Size: 5686 NspI r_CATG'y Cuts at: 1132 1229 2311 2947 3044 4589 1132 Size: 97 1082 636 97 1545 2229 Fragments arranged by size: 2229 1545 1082 636 97 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5686 PciI A'CATG_T Cuts at: 4585 4585 Size: 5686 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5686 PfoI T'CCnGG_A Cuts at: 2034 2430 2034 Size: 396 5290 PmeI GTTT'AAAC Cuts at: 2990 2990 Size: 5686 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3755 3787 5268 5300 3755 Size: 32 1481 32 4141 Fragments arranged by size: 4141 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2503 2503 Size: 5686 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5686 PsiI TTA'TAA Cuts at: 3256 3256 Size: 5686 PspOMI G'GGCC_C Cuts at: 2592 2592 Size: 5686 PstI C_TGCA'G Cuts at: 830 3038 830 Size: 2208 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5686 PvuII CAG'CTG Cuts at: 2471 2471 Size: 5686 SalI G'TCGA_C Cuts at: 1936 3028 1936 Size: 1092 4594 SapI GCTCTTCn'nnn_ Cuts at: 2625 2625 Size: 5686 SbfI CC_TGCA'GG Cuts at: 3038 3038 Size: 5686 ScaI AGT'ACT Cuts at: 3934 5646 3934 Size: 1712 3974 SexAI A'CCwGG_T Cuts at: 2640 2640 Size: 5686 SfcI C'TryA_G Cuts at: 826 1045 2970 3034 4169 4850 5041 826 Size: 219 1925 64 1135 681 191 1471 Fragments arranged by size: 1925 1471 1135 681 219 191 64 SfoI GGC'GCC Cuts at: 2160 2160 Size: 5686 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 SgrAI Cr'CCGG_yG Cuts at: 1700 2610 1700 Size: 910 4776 SmaI CCC'GGG Cuts at: 1872 2822 3013 1872 Size: 950 191 4545 Fragments arranged by size: 4545 950 191 SmlI C'TyrA_G Cuts at: 820 1017 1943 3790 4691 4953 820 Size: 197 926 1847 901 262 1553 Fragments arranged by size: 1847 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3477 493 Size: 2984 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5686 SphI G_CATG'C Cuts at: 1132 2311 3044 1132 Size: 1179 733 3774 Fragments arranged by size: 3774 1179 733 SspI AAT'ATT Cuts at: 5 52 3610 5 Size: 47 3558 2081 Fragments arranged by size: 3558 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5686 StyI C'CwwG_G Cuts at: 513 1065 2004 2763 3125 513 Size: 552 939 759 362 3074 Fragments arranged by size: 3074 939 759 552 362 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2718 3713 3730 3883 4068 4513 325 Size: 1255 1138 995 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1138 995 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3932 5644 96 Size: 274 80 33 51 1443 1955 1712 138 Fragments arranged by size: 1955 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2139 2171 5646 5678 2139 Size: 32 3475 32 2147 Fragments arranged by size: 3475 2147 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5686 XbaI T'CTAG_A Cuts at: 3022 3022 Size: 5686 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5686 XmaI C'CCGG_G Cuts at: 1870 2820 3011 1870 Size: 950 191 4545 Fragments arranged by size: 4545 950 191 XmnI GAAnn'nnTTC Cuts at: 2561 3815 2561 Size: 1254 4432 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2128 3494 276 Size: 53 83 186 936 594 1366 2468 Fragments arranged by size: 2468 1366 936 594 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI XcmI