################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccIII AclI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbrPI BbsI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaJI BsaWI BseAI BseRI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeIII HpaI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MunI NaeI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SapI SbfI ScaI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 573 GTCGAC GT^[AC][GT]AC AcsI 276 AAATTT [GA]^AATT[CT] AcyI 665 GGCGCC G[GA]^CG[CT]C ApoI 276 AAATTT [GA]^AATT[CT] AvaII 535 GGACC G^G[AT]CC BanI 665 GGCGCC G^G[CT][GA]CC BanII 697 GAGCCC G[GA]GC[CT]^C BbeI 665 GGCGCC GGCGC^C BbvCI 3 GCTGAGG CCTCAGC(-5/-2) BglII 42 AGATCT A^GATCT BmyI 697 GAGCCC G[AGT]GC[ACT]^C BpmI 446 CTCCAG CTGGAG(16/14) BsaHI 665 GGCGCC G[GA]^CG[CT]C BsgI 370 GTGCAG GTGCAG(16/14) BsiEI 680 CGACCG CG[GA][CT]^CG BsmAI 628 GAGAC GTCTC(1/5) Bsp1286I 697 GAGCCC G[AGT]GC[ACT]^C BssHII 716 GCGCGC G^CGCGC BssSI 182 CACGAG CACGAG(-5/-1) BstF5I 108 GGATG GGATG(2/0) BstYI 42 AGATCT [GA]^GATC[CT] Cfr13I 535 GGACC G^G[ACGT]CC DdeI 4 CTGAG C^T[ACGT]AG DraI 250 TTTAAA TTT^AAA FokI 108 GGATG GGATG(9/13) HaeII 665 GGCGCC [GA]GCGC^[CT] HapII 380 CCGG C^CGG HgaI 158 GCGTC GACGC(5/10) Hin1I 665 GGCGCC G[GA]^CG[CT]C HincII 573 GTCGAC GT[CT]^[GA]AC HindII 573 GTCGAC GT[CT]^[GA]AC HindIII 255 AAGCTT A^AGCTT HpaII 380 CCGG C^CGG KasI 665 GGCGCC G^GCGCC MaeIII 235 GTTAC ^GT[ACGT]AC MflI 42 AGATCT [GA]^GATC[CT] MslI 595 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 244 CAGCTG C[AC]G^C[GT]G MspI 380 CCGG C^CGG NarI 665 GGCGCC GG^CGCC PvuII 244 CAGCTG CAG^CTG SalI 573 GTCGAC G^TCGAC Sau96I 535 GGACC G^G[ACGT]CC SexAI 230 ACCAGGT A^CC[AT]GGT SfoI 665 GGCGCC GGC^GCC TaqI 574 TCGA T^CGA TthHB8I 574 TCGA T^CGA XhoII 42 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BsrI 36 CCAGT ACTGG(1/-1) 460 CCAGT BssKI 231 CCAGG ^CC[ACGT]GG 538 CCAGG BstNI 231 CCAGG CC^[AT]GG 538 CCAGG BstXI 460 CCAGTGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 581 CCAGCGTGGTGG Cac8I 151 GCTTGC GC[ACGT]^[ACGT]GC 716 GCGCGC DpnII 43 GATC ^GATC 130 GATC EcoRII 231 CCAGG ^CC[AT]GG 538 CCAGG FauI 390 GCGGG CCCGC(4/6) 562 GCGGG HinfI 266 GAGTC G^A[ACGT]TC 571 GAGTC HphI 88 GGTGA GGTGA(8/7) 215 TCACC MboI 43 GATC ^GATC 130 GATC MseI 124 TTAA T^TAA 251 TTAA MvaI 231 CCAGG CC^[AT]GG 538 CCAGG NdeII 43 GATC ^GATC 130 GATC NlaIII 163 CATG CATG^ 595 CATG NlaIV 534 GGGACC GG[ACGT]^[ACGT]CC 665 GGCGCC PleI 266 GAGTC GAGTC(4/5) 571 GAGTC Sau3AI 43 GATC ^GATC 130 GATC ScrFI 231 CCAGG CC^[ACGT]GG 538 CCAGG SfaNI 484 GATGC GCATC(5/9) 489 GATGC Tru9I 124 TTAA T^TAA 251 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AccII AciI AluI BbvI BsiYI BslI BsmFI BstUI CfoI EarI Fnu4HI HhaI HinP1I ItaI Ksp632I MboII MnlI MvnI MwoI TseI Tsp509I TspRI