################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HgaI Hin1I HpaII KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcsI 301 AAATTT [GA]^AATT[CT] AflIII 390 ACATGT A^C[GA][CT]GT AluI 362 AGCT AG^CT ApaI 645 GGGCCC GGGCC^C ApoI 301 AAATTT [GA]^AATT[CT] AspI 586 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 645 GGGCCC G[GA]GC[CT]^C BbsI 667 GTCTTC GAAGAC(2/6) BbvI 193 GCAGC GCAGC(8/12) BcgI 532 CGAAAACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 430 AGATCT A^GATCT BmyI 645 GGGCCC G[AGT]GC[ACT]^C BpuAI 667 GTCTTC GAAGAC(2/6) BsgI 596 GTGCAG GTGCAG(16/14) BsmFI 775 GTCCC GGGAC(10/14) Bsp1286I 645 GGGCCC G[AGT]GC[ACT]^C BspHI 693 TCATGA T^CATGA BspLU11I 390 ACATGT A^CATGT BstAPI 598 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 129 CATCC GGATG(2/0) BstXI 581 CCAAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 248 GCGC GCG^C EcoRV 346 GATATC GAT^ATC FauI 777 CCCGC CCCGC(4/6) FokI 129 CATCC GGATG(9/13) HhaI 248 GCGC GCG^C HinP1I 248 GCGC G^CGC HincII 735 GTTAAC GT[CT]^[GA]AC HindII 735 GTTAAC GT[CT]^[GA]AC HindIII 361 AAGCTT A^AGCTT HpaI 735 GTTAAC GTT^AAC MaeII 149 ACGT A^CGT NspI 390 ACATGT [GA]CATG^[CT] PflFI 586 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 693 TCATGA T^CATGA SfcI 677 CTATAG C^T[GA][CT]AG SmlI 315 CTCAAG C^T[CT][GA]AG StuI 272 AGGCCT AGG^CCT TaqI 531 TCGA T^CGA TseI 193 GCAGC G^C[AT]GC Tth111I 586 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 531 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BpmI 289 CTGGAG CTGGAG(16/14) 686 CTGGAG BsmAI 228 GTCTC GTCTC(1/5) 682 GTCTC BssKI 110 CCAGG ^CC[ACGT]GG 718 CCTGG BstNI 110 CCAGG CC^[AT]GG 718 CCTGG Cfr13I 645 GGGCC G^G[ACGT]CC 646 GGCCC DraII 644 GGGGCCC [GA]G^G[ACGT]CC[CT] 645 GGGCCCC EcoO109I 644 GGGGCCC [GA]G^G[ACGT]CC[CT] 645 GGGCCCC EcoRII 110 CCAGG ^CC[AT]GG 718 CCTGG Fnu4HI 193 GCAGC GC^[ACGT]GC 373 GCCGC ItaI 193 GCAGC GC^[ACGT]GC 373 GCCGC MboII 13 TCTTC GAAGA(8/7) 668 TCTTC MslI 148 CACGTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 694 CATGAATGTG MvaI 110 CCAGG CC^[AT]GG 718 CCTGG MwoI 354 GCTGGTTAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 598 GCAGGAGATGC PleI 54 GACTC GAGTC(4/5) 637 GACTC Sau96I 645 GGGCC G^G[ACGT]CC 646 GGCCC ScrFI 110 CCAGG CC^[ACGT]GG 718 CCTGG SfaNI 399 GATGC GCATC(5/9) 604 GATGC Tsp509I 78 AATT ^AATT 302 AATT XcmI 110 CCAGGCCATTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 278 CCAAACAAACACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AciI AlwI BsrI BstYI DdeI DpnII HaeIII HinfI HphI MaeIII MboI MflI MnlI MseI NdeII NlaIII NlaIV Sau3AI TfiI Tru9I Tsp45I TspRI XhoII