(Circular) (Six-base) MAPSORT of: pFN21AE4420.seq Check: 8733 from: 1 to: 6010 pFN21AE4420 6010 bp With 182 enzymes: SgfI * March 28, 2012 13:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2025 2025 Size: 6010 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3820 278 Size: 53 83 186 936 2284 2468 Fragments arranged by size: 2468 2284 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3353 1937 Size: 1416 4594 Acc65I G'GTAC_C Cuts at: 3331 3331 Size: 6010 AclI AA'CG_TT Cuts at: 1563 4137 4510 1563 Size: 2574 373 3063 Fragments arranged by size: 3063 2574 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4073 5457 5725 1632 Size: 2441 1384 268 1917 Fragments arranged by size: 2441 1917 1384 268 AfeI AGC'GCT Cuts at: 2453 2453 Size: 6010 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5813 AflIII A'CryG_T Cuts at: 1203 4909 5886 1203 Size: 3706 977 1327 Fragments arranged by size: 3706 1327 977 AgeI A'CCGG_T Cuts at: 4841 4841 Size: 6010 AhdI GACnn_n'nnGTC Cuts at: 4739 4739 Size: 6010 AleI CACnn'nnGTG Cuts at: 1166 3286 1166 Size: 2120 3890 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2762 1184 Size: 1578 4432 AlwNI CAG_nnn'CTG Cuts at: 3753 5325 3753 Size: 1572 4438 ApaI G_GGCC'C Cuts at: 2078 3042 3070 3195 2078 Size: 964 28 125 4893 Fragments arranged by size: 4893 964 125 28 ApaLI G'TGCA_C Cuts at: 4067 5223 4067 Size: 1156 4854 ApoI r'AATT_y Cuts at: 1493 3319 3545 3697 5806 1493 Size: 1826 226 152 2109 1697 Fragments arranged by size: 2109 1826 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4564 160 Size: 872 3532 1606 Fragments arranged by size: 3532 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 AvaI C'yCGr_G Cuts at: 1870 1943 3335 1870 Size: 73 1392 4545 Fragments arranged by size: 4545 1392 73 BamHI G'GATC_C Cuts at: 3340 3707 3340 Size: 367 5643 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2130 2243 2403 2558 3209 Size: 325 217 627 343 113 160 155 651 Cuts at: 3209 3280 3331 4786 618 Size: 71 51 1455 1842 Fragments arranged by size: 1842 1455 651 627 343 325 217 160 155 113 71 51 BanII G_rGCy'C Cuts at: 1391 2078 3042 3070 3195 3329 1391 Size: 687 964 28 125 134 4072 Fragments arranged by size: 4072 964 687 134 125 28 BbeI G_GCGC'C Cuts at: 2407 2407 Size: 6010 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2161 928 Size: 971 262 4777 Fragments arranged by size: 4777 971 262 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4201 4235 1903 Size: 34 2264 34 3678 Fragments arranged by size: 3678 2264 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3902 5112 3902 Size: 1210 4800 BclI T'GATC_A Cuts at: 1548 1548 Size: 6010 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2321 2552 2601 Size: 107 122 71 631 605 649 231 49 Cuts at: 2601 2673 2945 4621 136 Size: 72 272 1676 1525 Fragments arranged by size: 1676 1525 649 631 605 272 231 122 107 72 71 49 BglII A'GATC_T Cuts at: 2868 6005 2868 Size: 3137 2873 BlpI GC'TnA_GC Cuts at: 3427 3427 Size: 6010 Bme1580I G_kGCm'C Cuts at: 1790 2078 2246 2464 2581 2669 2686 2728 2768 Size: 288 168 218 117 88 17 42 40 Cuts at: 2768 3042 3070 3195 3214 4071 5227 1790 Size: 274 28 125 19 857 1156 2573 Fragments arranged by size: 2573 1156 857 288 274 218 168 125 117 88 42 40 28 19 17 BmgBI CAC'GTC Cuts at: 1318 2155 1318 Size: 837 5173 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2119 2277 2401 Size: 416 53 83 399 24 702 158 124 Cuts at: 2401 2434 3044 3741 4699 442 Size: 33 610 697 958 1753 Fragments arranged by size: 1753 958 702 697 610 416 399 158 124 83 53 33 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6010 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2978 2978 Size: 6010 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2549 2780 2933 2973 4670 1239 Size: 917 393 231 153 40 1697 2579 Fragments arranged by size: 2579 1697 917 393 231 153 40 Bpu10I CC'TnA_GC Cuts at: 3197 3197 Size: 6010 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4135 5000 5298 4135 Size: 865 298 4847 Fragments arranged by size: 4847 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4673 882 Size: 617 3174 2219 Fragments arranged by size: 3174 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3801 493 Size: 711 2597 2702 Fragments arranged by size: 2702 2597 711 BsaBI GATnn'nnATC Cuts at: 6004 6004 Size: 6010 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2404 3817 4199 4834 Size: 53 83 186 936 871 1413 382 635 Cuts at: 4834 275 Size: 1451 Fragments arranged by size: 1451 1413 936 871 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4443 4841 5115 5262 5673 4443 Size: 398 274 147 411 4780 Fragments arranged by size: 4780 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2264 2294 1178 Size: 30 165 30 861 30 4894 Fragments arranged by size: 4894 861 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2487 3131 3134 1186 Size: 1301 644 3 4062 Fragments arranged by size: 4062 1301 644 3 BseYI C'CCAG_C Cuts at: 2235 2239 2412 2569 2735 2973 5213 5709 2235 Size: 4 173 157 166 238 2240 496 2536 Fragments arranged by size: 2536 2240 496 238 173 166 157 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2523 1163 Size: 573 787 4650 Fragments arranged by size: 4650 787 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3460 4221 5249 1540 Size: 275 186 1459 761 1028 2301 Fragments arranged by size: 2301 1459 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2919 2964 3095 4071 4156 5227 2919 Size: 45 131 976 85 1071 3702 Fragments arranged by size: 3702 1071 976 131 85 45 BsmI GAATG_Cn' Cuts at: 2119 2305 2810 3521 3614 2119 Size: 186 505 711 93 4515 Fragments arranged by size: 4515 711 505 186 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2078 2246 2464 2581 2669 2686 2728 Size: 399 288 168 218 117 88 17 42 Cuts at: 2728 2768 2919 2964 3042 3070 3095 3195 3214 Size: 40 151 45 78 28 25 100 19 Cuts at: 3214 3329 4071 4156 5227 1391 Size: 115 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 288 218 168 151 117 115 100 88 85 78 45 42 40 28 25 19 17 BspEI T'CCGG_A Cuts at: 5673 5673 Size: 6010 BspHI T'CATG_A Cuts at: 3899 5629 3899 Size: 1730 4280 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2025 2365 3365 844 Size: 1181 340 1000 3489 Fragments arranged by size: 3489 1181 1000 340 BsrBI CCG'CTC Cuts at: 1394 1823 3295 3897 1394 Size: 429 1472 602 3507 Fragments arranged by size: 3507 1472 602 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4505 4679 65 Size: 1183 3257 174 1396 Fragments arranged by size: 3257 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2091 2445 2657 2848 4654 4841 1700 Size: 110 281 354 212 191 1806 187 2869 Fragments arranged by size: 2869 1806 354 281 212 191 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6010 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6010 BssSI C'ACGA_G Cuts at: 4070 5082 4070 Size: 1012 4998 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6010 BstBI TT'CG_AA Cuts at: 4814 4814 Size: 6010 BstEII G'GTnAC_C Cuts at: 1184 2834 1184 Size: 1650 4360 BstYI r'GATC_y Cuts at: 1972 2868 3340 3707 4092 4109 5561 5647 5659 Size: 896 472 367 385 17 1452 86 12 Cuts at: 5659 6005 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 896 472 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2499 2499 Size: 6010 BtgI C'CryG_G Cuts at: 513 1065 2004 2203 3100 3284 513 Size: 552 939 199 897 184 3239 Fragments arranged by size: 3239 939 897 552 199 184 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5271 Fragments arranged by size: 5271 683 56 BtsI GCAGTG_nn' Cuts at: 2579 2760 3526 4320 4340 2579 Size: 181 766 794 20 4249 Fragments arranged by size: 4249 794 766 181 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6010 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5975 DraI TTT'AAA Cuts at: 3314 3314 Size: 6010 DraIII CAC_nnn'GTG Cuts at: 2127 2149 2800 2127 Size: 22 651 5337 Fragments arranged by size: 5337 651 22 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2159 5017 809 Size: 507 843 2858 1802 Fragments arranged by size: 2858 1802 843 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2489 2659 2802 Size: 54 1425 50 275 18 659 170 143 Cuts at: 2802 3097 3103 3457 4346 5975 8 Size: 295 6 354 889 1629 43 Fragments arranged by size: 1629 1425 889 659 354 295 275 170 143 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 3457 1537 Size: 275 1645 4090 Fragments arranged by size: 4090 1645 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2042 3293 3940 1353 Size: 689 1251 647 3423 Fragments arranged by size: 3423 1251 689 647 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3104 4597 4971 5117 387 Size: 1064 138 1515 1493 374 146 1280 Fragments arranged by size: 1515 1493 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2549 2780 2933 2973 4073 4670 Size: 393 524 393 231 153 40 1100 597 Cuts at: 4670 5457 5725 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1100 787 597 524 393 393 268 231 153 40 EcoO109I rG'GnC_Cy Cuts at: 2074 2075 3038 3039 3066 3067 3192 2074 Size: 1 963 1 27 1 125 4892 Fragments arranged by size: 4892 963 125 27 1 1 1 EcoRI G'AATT_C Cuts at: 3319 3697 3319 Size: 378 5632 FspI TGC'GCA Cuts at: 4516 4516 Size: 6010 HaeII r_GCGC'y Cuts at: 1678 2407 2455 5157 1678 Size: 729 48 2702 2531 Fragments arranged by size: 2702 2531 729 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4651 4683 4725 4757 1373 Size: 32 135 32 3079 32 42 32 2626 Fragments arranged by size: 3079 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3354 3600 669 Size: 988 281 1416 246 3079 Fragments arranged by size: 3079 1416 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6010 HpaI GTT'AAC Cuts at: 3600 3600 Size: 6010 KasI G'GCGC_C Cuts at: 2403 2403 Size: 6010 KpnI G_GTAC'C Cuts at: 3335 3335 Size: 6010 MfeI C'AATT_G Cuts at: 3609 3609 Size: 6010 MluI A'CGCG_T Cuts at: 5886 5886 Size: 6010 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5124 5308 1305 Size: 3819 184 2007 Fragments arranged by size: 3819 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2491 10 Size: 54 1425 343 659 3529 Fragments arranged by size: 3529 1425 659 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2744 3286 3968 4327 4486 518 Size: 648 1578 542 682 359 159 2042 Fragments arranged by size: 2042 1578 682 648 542 359 159 MspA1I CmG'CkG Cuts at: 1606 2205 2239 2569 2951 3108 3426 4103 5251 Size: 599 34 330 382 157 318 677 1148 Cuts at: 5251 5496 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 599 382 330 318 245 157 34 NarI GG'CG_CC Cuts at: 2404 2404 Size: 6010 NcoI C'CATG_G Cuts at: 513 1065 2004 3100 513 Size: 552 939 1096 3423 Fragments arranged by size: 3423 1096 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6010 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6010 NotI GC'GGCC_GC Cuts at: 3457 3457 Size: 6010 NspI r_CATG'y Cuts at: 1132 1229 2749 3307 3368 4913 1132 Size: 97 1520 558 61 1545 2229 Fragments arranged by size: 2229 1545 1520 558 97 61 PasI CC'CwG_GG Cuts at: 1348 2189 2941 1348 Size: 841 752 4417 Fragments arranged by size: 4417 841 752 PciI A'CATG_T Cuts at: 4909 4909 Size: 6010 PflMI CCAn_nnn'nTGG Cuts at: 1108 2109 1108 Size: 1001 5009 PfoI T'CCnGG_A Cuts at: 2334 2334 Size: 6010 PmeI GTTT'AAAC Cuts at: 3314 3314 Size: 6010 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4079 4111 5592 5624 4079 Size: 32 1481 32 4465 Fragments arranged by size: 4465 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6010 PsiI TTA'TAA Cuts at: 3580 3580 Size: 6010 PspOMI G'GGCC_C Cuts at: 2074 3038 3066 3191 2074 Size: 964 28 125 4893 Fragments arranged by size: 4893 964 125 28 PstI C_TGCA'G Cuts at: 830 3080 3362 830 Size: 2250 282 3478 Fragments arranged by size: 3478 2250 282 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6010 PvuII CAG'CTG Cuts at: 2239 2569 2951 2239 Size: 330 382 5298 Fragments arranged by size: 5298 382 330 SacII CC_GC'GG Cuts at: 2206 2206 Size: 6010 SalI G'TCGA_C Cuts at: 1936 3352 1936 Size: 1416 4594 SapI GCTCTTCn'nnn_ Cuts at: 3293 3293 Size: 6010 SbfI CC_TGCA'GG Cuts at: 3362 3362 Size: 6010 ScaI AGT'ACT Cuts at: 4258 5970 4258 Size: 1712 4298 SfcI C'TryA_G Cuts at: 826 1045 2227 3076 3358 4493 5174 5365 826 Size: 219 1182 849 282 1135 681 191 1471 Fragments arranged by size: 1471 1182 1135 849 681 282 219 191 SfoI GGC'GCC Cuts at: 2405 2405 Size: 6010 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6010 SmaI CCC'GGG Cuts at: 1872 3337 1872 Size: 1465 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4114 5015 5277 820 Size: 197 926 2171 901 262 1553 Fragments arranged by size: 2171 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3801 493 Size: 3308 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6010 SphI G_CATG'C Cuts at: 1132 3368 1132 Size: 2236 3774 SspI AAT'ATT Cuts at: 5 52 3934 5 Size: 47 3882 2081 Fragments arranged by size: 3882 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6010 StyI C'CwwG_G Cuts at: 513 1065 2004 2062 2433 2991 3100 3449 513 Size: 552 939 58 371 558 109 349 3074 Fragments arranged by size: 3074 939 558 552 371 349 109 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2138 2956 4037 4054 4207 4392 4837 Size: 1255 558 818 1081 17 153 185 445 Cuts at: 4837 325 Size: 1498 Fragments arranged by size: 1498 1255 1081 818 558 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4256 5968 96 Size: 274 80 33 51 1443 2279 1712 138 Fragments arranged by size: 2279 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5970 6002 5970 Size: 32 5978 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6010 XbaI T'CTAG_A Cuts at: 3346 3346 Size: 6010 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2948 3179 2948 Size: 231 5779 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6010 XmaI C'CCGG_G Cuts at: 1870 3335 1870 Size: 1465 4545 XmnI GAAnn'nnTTC Cuts at: 4139 4139 Size: 6010 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3818 276 Size: 53 83 186 936 2284 2468 Fragments arranged by size: 2468 2284 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI