################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BbeI BbrPI BbsI BcgI BfaI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaI BseAI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI BspEI BssSI Bst1107I BstBI BstZ17I CpoI DraI DraIII EagI Eam1105I EclXI Eco52I EcoNI EcoRI EcoRV FseI FspI HincII HindII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MseI MunI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tru9I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AfaI 1023 GTAC GT^AC AflIII 569 ACATGT A^C[GA][CT]GT AgeI 36 ACCGGT A^CCGGT Aor51HI 1055 AGCGCT AGC^GCT ApaI 1390 GGGCCC GGGCC^C AspI 802 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1305 GGATCC G^GATCC BanI 1037 GGCACC G^G[CT][GA]CC BbvCI 338 CCTCAGC CCTCAGC(-5/-2) BciVI 404 GGATAC GTATCC(6/5) BcnI 813 CCGGG CC^[GC]GG BseRI 211 GAGGAG GAGGAG(10/8) Bsp1407I 1022 TGTACA T^GTACA BspDI 1264 ATCGAT AT^CGAT BspHI 590 TCATGA T^CATGA BspLU11I 569 ACATGT A^CATGT BspMI 1255 ACCTGC ACCTGC(4/8) BsrBI 817 GAGCGG CCGCTC(-3/-3) BsrGI 1022 TGTACA T^GTACA BssHII 1518 GCGCGC G^CGCGC BstAPI 1250 GCATTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1305 GGATCC [GA]^GATC[CT] ClaI 1264 ATCGAT AT^CGAT DrdI 231 GACCCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 367 CTCTTC CTCTTC(1/4) Eco47III 1055 AGCGCT AGC^GCT EcoT22I 615 ATGCAT ATGCA^T HindIII 1563 AAGCTT A^AGCTT Ksp632I 367 CTCTTC CTCTTC(1/4) MflI 1305 GGATCC [GA]^GATC[CT] NaeI 137 GCCGGC GCC^GGC NciI 813 CCGGG CC^[GC]GG NgoMIV 137 GCCGGC G^CCGGC NsiI 615 ATGCAT ATGCA^T NspI 569 ACATGT [GA]CATG^[CT] PflFI 802 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 720 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 36 ACCGGT A^CCGGT RcaI 590 TCATGA T^CATGA RsaI 1023 GTAC GT^AC SacI 679 GAGCTC GAGCT^C SspBI 1022 TGTACA T^GTACA Tsp509I 1508 AATT ^AATT Tth111I 802 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 720 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 163 CCAAAACGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1305 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 605 GTCTAC GT^[AC][GT]AC 1315 GTCTAC AccII 1093 CGCG CG^CG 1519 CGCG AcyI 453 GACGCC G[GA]^CG[CT]C 821 GGCGTC AocI 1164 CCTGAGG CC^T[ACGT]AGG 1309 CCTGAGG AspHI 577 GAGCAC G[AT]GC[AT]^C 679 GAGCTC BclI 197 TGATCA T^GATCA 1511 TGATCA BlpI 9 GCTGAGC GC^T[ACGT]AGC 909 GCTGAGC BpmI 142 CTCCAG CTGGAG(16/14) 718 CTCCAG Bpu1102I 9 GCTGAGC GC^T[ACGT]AGC 909 GCTGAGC BsaHI 453 GACGCC G[GA]^CG[CT]C 821 GGCGTC BsgI 1203 CTGCAC GTGCAG(16/14) 1459 CTGCAC BsiHKAI 577 GAGCAC G[AT]GC[AT]^C 679 GAGCTC BsrDI 348 CATTGC GCAATG(2/0) 43 GCAATG BsrFI 36 ACCGGT [GA]^CCGG[CT] 137 GCCGGC BstEII 184 GGTCACC G^GT[ACGT]ACC 1358 GGTCACC BstPI 184 GGTCACC G^GT[ACGT]ACC 1358 GGTCACC BstUI 1093 CGCG CG^CG 1519 CGCG Bsu36I 1164 CCTGAGG CC^T[ACGT]AGG 1309 CCTGAGG CelII 9 GCTGAGC GC^T[ACGT]AGC 909 GCTGAGC Cfr10I 36 ACCGGT [GA]^CCGG[CT] 137 GCCGGC Eco81I 1164 CCTGAGG CC^T[ACGT]AGG 1309 CCTGAGG EcoO65I 184 GGTCACC G^GT[ACGT]ACC 1358 GGTCACC FbaI 197 TGATCA T^GATCA 1511 TGATCA HaeII 761 GGCGCT [GA]GCGC^[CT] 1055 AGCGCT Hin1I 453 GACGCC G[GA]^CG[CT]C 821 GGCGTC HinfI 226 GACTC G^A[ACGT]TC 1235 GAGTC MspA1I 341 CAGCTG C[AC]G^C[GT]G 1129 CAGCTG MvnI 1093 CGCG CG^CG 1519 CGCG NcoI 52 CCATGG C^CATGG 1421 CCATGG PleI 226 GACTC GAGTC(4/5) 1235 GAGTC PpuMI 276 GGGTCCC [GA]G^G[AT]CC[CT] 1016 GGGTCCT PvuII 341 CAGCTG CAG^CTG 1129 CAGCTG SfcI 206 CTACAG C^T[GA][CT]AG 607 CTACAG TaqI 229 TCGA T^CGA 1265 TCGA TthHB8I 229 TCGA T^CGA 1265 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AluI AlwI AlwNI AvaII BanII BbvI BmyI BsaJI BsaWI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeIII MboI MboII MnlI MslI MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TseI Tsp45I TspRI