################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII BalI BamHI BanI BanII BbeI BcgI BciVI BclI BcnI BfrI BglI BglII BlpI BmyI BpmI Bpu1102I BsaBI BsaHI BsaI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HaeIII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeIII MamI MfeI MluI MluNI MscI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I TspRI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccIII 6 TCCGGA T^CCGGA AciI 592 CCGC CCGC(-3/-1) AluI 588 AGCT AG^CT AlwNI 286 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 430 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 52 CCTAGG C^CTAGG BbrPI 268 CACGTG CAC^GTG BbsI 382 GAAGAC GAAGAC(2/6) BbvCI 177 GCTGAGG CCTCAGC(-5/-2) BbvI 344 GCAGC GCAGC(8/12) BfaI 53 CTAG C^TAG BlnI 52 CCTAGG C^CTAGG BpuAI 382 GAAGAC GAAGAC(2/6) BsaAI 268 CACGTG [CT]AC^GT[GA] BsaWI 6 TCCGGA [AT]^CCGG[AT] BseAI 6 TCCGGA T^CCGGA BsmAI 186 GAGAC GTCTC(1/5) BspEI 6 TCCGGA T^CCGGA BssKI 285 CCAGG ^CC[ACGT]GG BstNI 285 CCAGG CC^[AT]GG BstYI 48 AGATCC [GA]^GATC[CT] Cac8I 263 GCCAGC GC[ACGT]^[ACGT]GC EarI 355 CTCTTC CTCTTC(1/4) EcoRII 285 CCAGG ^CC[AT]GG EcoT14I 52 CCTAGG C^C[AT][AT]GG Fnu4HI 344 GCAGC GC^[ACGT]GC HapII 7 CCGG C^CGG HgaI 279 GCGTC GACGC(5/10) HpaII 7 CCGG C^CGG HphI 351 TCACC GGTGA(8/7) ItaI 344 GCAGC GC^[ACGT]GC Ksp632I 355 CTCTTC CTCTTC(1/4) MaeI 53 CTAG C^TAG MflI 48 AGATCC [GA]^GATC[CT] MroI 6 TCCGGA T^CCGGA MseI 451 TTAA T^TAA MspI 7 CCGG C^CGG MvaI 285 CCAGG CC^[AT]GG NspI 119 GCATGT [GA]CATG^[CT] PleI 188 GACTC GAGTC(4/5) PmaCI 268 CACGTG CAC^GTG PmlI 268 CACGTG CAC^GTG ScaI 416 AGTACT AGT^ACT ScrFI 285 CCAGG CC^[ACGT]GG SfcI 316 CTACAG C^T[GA][CT]AG StyI 52 CCTAGG C^C[AT][AT]GG TaqI 45 TCGA T^CGA Tru9I 451 TTAA T^TAA TseI 344 GCAGC G^C[AT]GC TthHB8I 45 TCGA T^CGA XhoII 48 AGATCC [GA]^GATC[CT] XmnI 430 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AlwI 49 GATCC GGATC(4/5) 273 GGATC BsaJI 52 CCTAGG C^C[ACGT][ACGT]GG 285 CCAGGG BsiYI 264 CCAGCACGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 285 CCAGGGTCTGG BslI 264 CCAGCACGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 285 CCAGGGTCTGG BsmI 321 GAATGC GAATGC(1/-1) 78 GCATTC DpnII 49 GATC ^GATC 274 GATC EcoRI 166 GAATTC G^AATTC 375 GAATTC MaeII 269 ACGT A^CGT 462 ACGT MboI 49 GATC ^GATC 274 GATC MslI 544 CACAGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 566 CACATGGATG NdeII 49 GATC ^GATC 274 GATC PflMI 264 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 285 CCAGGGTCTGG Sau3AI 49 GATC ^GATC 274 GATC Van91I 264 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 285 CCAGGGTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### AcsI AfaI ApoI BstF5I DdeI FokI HinfI MboII MnlI NlaIII RsaI TfiI Tsp509I