(Circular) (Six-base) MAPSORT of: pFN21AE1386.seq Check: 3492 from: 1 to: 5320 pFN21AE1386 5320 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3130 278 Size: 53 83 186 936 1594 2468 Fragments arranged by size: 2468 1594 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2331 2663 1937 Size: 394 332 4594 Fragments arranged by size: 4594 394 332 Acc65I G'GTAC_C Cuts at: 2641 2641 Size: 5320 AclI AA'CG_TT Cuts at: 1563 3447 3820 1563 Size: 1884 373 3063 Fragments arranged by size: 3063 1884 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2115 3383 4767 5035 1632 Size: 483 1268 1384 268 1917 Fragments arranged by size: 1917 1384 1268 483 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5123 AflIII A'CryG_T Cuts at: 1203 4219 5196 1203 Size: 3016 977 1327 Fragments arranged by size: 3016 1327 977 AgeI A'CCGG_T Cuts at: 4151 4151 Size: 5320 AhdI GACnn_n'nnGTC Cuts at: 2148 4049 2148 Size: 1901 3419 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5320 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5320 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2010 2042 2010 Size: 32 5288 AlwNI CAG_nnn'CTG Cuts at: 3063 4635 3063 Size: 1572 3748 ApaI G_GGCC'C Cuts at: 2101 2101 Size: 5320 ApaLI G'TGCA_C Cuts at: 3377 4533 3377 Size: 1156 4164 ApoI r'AATT_y Cuts at: 1493 2629 2855 3007 5116 1493 Size: 1136 226 152 2109 1697 Fragments arranged by size: 2109 1697 1136 226 152 AseI AT'TA_AT Cuts at: 160 1032 3874 160 Size: 872 2842 1606 Fragments arranged by size: 2842 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5320 AvaI C'yCGr_G Cuts at: 1870 1943 2645 1870 Size: 73 702 4545 Fragments arranged by size: 4545 702 73 BamHI G'GATC_C Cuts at: 2650 3017 2650 Size: 367 4953 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2045 2641 4096 618 Size: 325 217 627 258 596 1455 1842 Fragments arranged by size: 1842 1455 627 596 325 258 217 BanII G_rGCy'C Cuts at: 1391 2101 2109 2639 1391 Size: 710 8 530 4072 Fragments arranged by size: 4072 710 530 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2338 2590 928 Size: 971 439 252 3658 Fragments arranged by size: 3658 971 439 252 BbvCI CC'TCA_GC Cuts at: 2450 2582 2450 Size: 132 5188 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2308 2342 3511 3545 1903 Size: 34 371 34 1169 34 3678 Fragments arranged by size: 3678 1169 371 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2481 3212 4422 2481 Size: 731 1210 3379 Fragments arranged by size: 3379 1210 731 BclI T'GATC_A Cuts at: 1548 1548 Size: 5320 BfrBI ATG'CAT Cuts at: 2167 2167 Size: 5320 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3931 136 Size: 107 122 71 631 605 2259 1525 Fragments arranged by size: 2259 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5315 5315 Size: 5320 BlpI GC'TnA_GC Cuts at: 2737 2737 Size: 5320 Bme1580I G_kGCm'C Cuts at: 1790 2101 3381 4537 1790 Size: 311 1280 1156 2573 Fragments arranged by size: 2573 1280 1156 311 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5320 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2419 3051 4009 Size: 416 53 83 399 24 1002 632 958 Cuts at: 4009 442 Size: 1753 Fragments arranged by size: 1753 1002 958 632 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5320 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2252 2260 3980 1239 Size: 1013 8 1720 2579 Fragments arranged by size: 2579 1720 1013 8 Bpu10I CC'TnA_GC Cuts at: 2450 2582 2450 Size: 132 5188 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2305 3445 4310 4608 2305 Size: 1140 865 298 3017 Fragments arranged by size: 3017 1140 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2205 2532 3983 882 Size: 617 706 327 1451 2219 Fragments arranged by size: 2219 1451 706 617 327 BsaAI yAC'GTr Cuts at: 493 1204 3111 493 Size: 711 1907 2702 Fragments arranged by size: 2702 1907 711 BsaBI GATnn'nnATC Cuts at: 5314 5314 Size: 5320 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3127 3509 4144 275 Size: 53 83 186 936 1594 382 635 1451 Fragments arranged by size: 1594 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3753 4151 4425 4572 4983 3753 Size: 398 274 147 411 4090 Fragments arranged by size: 4090 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2046 2076 1178 Size: 30 165 30 643 30 4422 Fragments arranged by size: 4422 643 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2371 2410 2438 1186 Size: 865 320 39 28 4068 Fragments arranged by size: 4068 865 320 39 28 BseYI C'CCAG_C Cuts at: 2216 4523 5019 2216 Size: 2307 496 2517 Fragments arranged by size: 2517 2307 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2480 1163 Size: 573 744 4003 Fragments arranged by size: 4003 744 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2770 3531 4559 1540 Size: 275 186 769 761 1028 2301 Fragments arranged by size: 2301 1028 769 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3381 3466 4537 3381 Size: 85 1071 4164 Fragments arranged by size: 4164 1071 85 BsmI GAATG_Cn' Cuts at: 2831 2924 2831 Size: 93 5227 BsmBI CGTCTCn'nnnn_ Cuts at: 2027 2027 Size: 5320 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2101 2109 2639 3381 3466 4537 1391 Size: 399 311 8 530 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 530 399 311 85 8 BspEI T'CCGG_A Cuts at: 4983 4983 Size: 5320 BspHI T'CATG_A Cuts at: 3209 4939 3209 Size: 1730 3590 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2200 2675 844 Size: 1356 475 3489 Fragments arranged by size: 3489 1356 475 BsrBI CCG'CTC Cuts at: 1394 1823 3207 1394 Size: 429 1384 3507 Fragments arranged by size: 3507 1384 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3815 3989 65 Size: 1183 2567 174 1396 Fragments arranged by size: 2567 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3964 4151 1700 Size: 110 2154 187 2869 Fragments arranged by size: 2869 2154 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5320 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5320 BssSI C'ACGA_G Cuts at: 3380 4392 3380 Size: 1012 4308 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5320 BstBI TT'CG_AA Cuts at: 4124 4124 Size: 5320 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5320 BstXI CCAn_nnnn'nTGG Cuts at: 2561 2561 Size: 5320 BstYI r'GATC_y Cuts at: 1972 2650 3017 3402 3419 4871 4957 4969 5315 Size: 678 367 385 17 1452 86 12 346 Cuts at: 5315 1972 Size: 1977 Fragments arranged by size: 1977 1452 678 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2509 513 Size: 552 1444 3324 Fragments arranged by size: 3324 1444 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4581 Fragments arranged by size: 4581 683 56 BtsI GCAGTG_nn' Cuts at: 2231 2836 3630 3650 2231 Size: 605 794 20 3901 Fragments arranged by size: 3901 794 605 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5320 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5285 DraI TTT'AAA Cuts at: 2624 2624 Size: 5320 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4327 809 Size: 507 3011 1802 Fragments arranged by size: 3011 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2767 3656 5285 Size: 54 1425 50 275 18 937 889 1629 Cuts at: 5285 8 Size: 43 Fragments arranged by size: 1629 1425 937 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2767 1537 Size: 275 955 4090 Fragments arranged by size: 4090 955 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2030 2420 3250 1353 Size: 677 390 830 3423 Fragments arranged by size: 3423 830 677 390 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3907 4281 4427 387 Size: 1064 138 2318 374 146 1280 Fragments arranged by size: 2318 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 2252 2260 3383 3980 4767 5035 Size: 393 483 137 8 1123 597 787 268 Cuts at: 5035 1239 Size: 1524 Fragments arranged by size: 1524 1123 787 597 483 393 268 137 8 EcoO109I rG'GnC_Cy Cuts at: 2097 2097 Size: 5320 EcoRI G'AATT_C Cuts at: 2629 3007 2629 Size: 378 4942 FspI TGC'GCA Cuts at: 3826 3826 Size: 5320 HaeII r_GCGC'y Cuts at: 1678 4467 1678 Size: 2789 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2134 2166 2264 2296 2455 Size: 32 135 32 562 32 98 32 159 Cuts at: 2455 2487 2531 2563 3961 3993 4035 4067 1373 Size: 32 44 32 1398 32 42 32 2626 Fragments arranged by size: 2626 1398 562 159 135 98 44 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2664 2910 669 Size: 988 281 726 246 3079 Fragments arranged by size: 3079 988 726 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5320 HpaI GTT'AAC Cuts at: 2910 2910 Size: 5320 KpnI G_GTAC'C Cuts at: 2645 2645 Size: 5320 MfeI C'AATT_G Cuts at: 2919 2919 Size: 5320 MluI A'CGCG_T Cuts at: 5196 5196 Size: 5320 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4434 4618 1305 Size: 3129 184 2007 Fragments arranged by size: 3129 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3498 Fragments arranged by size: 3498 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2559 3278 3637 3796 518 Size: 648 1393 719 359 159 2042 Fragments arranged by size: 2042 1393 719 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2135 2736 3413 4561 4806 1606 Size: 529 601 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 601 529 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4768 NdeI CA'TA_TG Cuts at: 387 387 Size: 5320 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5320 NotI GC'GGCC_GC Cuts at: 2767 2767 Size: 5320 NsiI A_TGCA'T Cuts at: 2169 2169 Size: 5320 NspI r_CATG'y Cuts at: 1132 1229 2678 4223 1132 Size: 97 1449 1545 2229 Fragments arranged by size: 2229 1545 1449 97 PasI CC'CwG_GG Cuts at: 1348 2101 2533 1348 Size: 753 432 4135 Fragments arranged by size: 4135 753 432 PciI A'CATG_T Cuts at: 4219 4219 Size: 5320 PflMI CCAn_nnn'nTGG Cuts at: 1108 2464 1108 Size: 1356 3964 PfoI T'CCnGG_A Cuts at: 2268 2268 Size: 5320 PmeI GTTT'AAAC Cuts at: 2624 2624 Size: 5320 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3389 3421 4902 4934 3389 Size: 32 1481 32 3775 Fragments arranged by size: 3775 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5320 PsiI TTA'TAA Cuts at: 2890 2890 Size: 5320 PspOMI G'GGCC_C Cuts at: 2097 2097 Size: 5320 PstI C_TGCA'G Cuts at: 830 2484 2672 830 Size: 1654 188 3478 Fragments arranged by size: 3478 1654 188 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5320 PvuII CAG'CTG Cuts at: 2135 2135 Size: 5320 RsrII CG'GwC_CG Cuts at: 2254 2254 Size: 5320 SalI G'TCGA_C Cuts at: 1936 2662 1936 Size: 726 4594 SapI GCTCTTCn'nnn_ Cuts at: 2420 2420 Size: 5320 SbfI CC_TGCA'GG Cuts at: 2672 2672 Size: 5320 ScaI AGT'ACT Cuts at: 2593 3568 5280 2593 Size: 975 1712 2633 Fragments arranged by size: 2633 1712 975 SfcI C'TryA_G Cuts at: 826 1045 2480 2668 3803 4484 4675 826 Size: 219 1435 188 1135 681 191 1471 Fragments arranged by size: 1471 1435 1135 681 219 191 188 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5320 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5320 SmaI CCC'GGG Cuts at: 1872 2647 1872 Size: 775 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2284 3424 4325 4587 820 Size: 197 926 341 1140 901 262 1553 Fragments arranged by size: 1553 1140 926 901 341 262 197 SnaBI TAC'GTA Cuts at: 493 3111 493 Size: 2618 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5320 SphI G_CATG'C Cuts at: 1132 2678 1132 Size: 1546 3774 SspI AAT'ATT Cuts at: 5 52 3244 5 Size: 47 3192 2081 Fragments arranged by size: 3192 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5320 StyI C'CwwG_G Cuts at: 513 1065 2033 2370 2759 513 Size: 552 968 337 389 3074 Fragments arranged by size: 3074 968 552 389 337 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2546 2567 3347 3364 3517 3702 4147 Size: 1255 966 21 780 17 153 185 445 Cuts at: 4147 325 Size: 1498 Fragments arranged by size: 1498 1255 966 780 445 185 153 21 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2591 3566 5278 Size: 274 80 33 51 1443 614 975 1712 Cuts at: 5278 96 Size: 138 Fragments arranged by size: 1712 1443 975 614 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5280 5312 5280 Size: 32 5288 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5320 XbaI T'CTAG_A Cuts at: 2656 2656 Size: 5320 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2108 2108 Size: 5320 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5320 XmaI C'CCGG_G Cuts at: 1870 2645 1870 Size: 775 4545 XmnI GAAnn'nnTTC Cuts at: 3449 3449 Size: 5320 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3128 276 Size: 53 83 186 936 1594 2468 Fragments arranged by size: 2468 1594 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI