Restriction map for
pFN21AE3915
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# NO CUT ENZYMES ( 182 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII BamHI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlpI BpmI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsmAI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HaeII HapII HgaI Hin1I HincII
HindII HindIII HinfI HpaI HpaII
HphI KasI KpnI KspI MamI
MfeI MluI MroI MseI MspI
MunI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tru9I Tsp509I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AlwI 341 GATCC GGATC(4/5)
AvrII 138 CCTAGG C^CTAGG
BalI 200 TGGCCA TGG^CCA
BanI 171 GGTGCC G^G[CT][GA]CC
BbsI 93 GTCTTC GAAGAC(2/6)
BlnI 138 CCTAGG C^CTAGG
BmyI 172 GTGCCC G[AGT]GC[ACT]^C
BpuAI 93 GTCTTC GAAGAC(2/6)
BsaAI 237 CACGTA [CT]AC^GT[GA]
BsiWI 239 CGTACG C^GTACG
BsmFI 135 GTCCC GGGAC(10/14)
BsmI 333 GAATGC GAATGC(1/-1)
Bsp1286I 172 GTGCCC G[AGT]GC[ACT]^C
BstYI 340 AGATCC [GA]^GATC[CT]
Cfr13I 127 GGGCC G^G[ACGT]CC
DdeI 115 CTCAG C^T[ACGT]AG
DsaI 260 CCATGG C^C[GA][CT]GG
EarI 211 GAAGAG CTCTTC(1/4)
FauI 34 CCCGC CCCGC(4/6)
Ksp632I 211 GAAGAG CTCTTC(1/4)
MaeIII 119 GTGAC ^GT[ACGT]AC
MflI 340 AGATCC [GA]^GATC[CT]
MluNI 200 TGGCCA TGG^CCA
MscI 200 TGGCCA TGG^CCA
MslI 78 CACCAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 43 GCCCTCAACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 260 CCATGG C^CATGG
NlaIV 171 GGTGCC GG[ACGT]^[ACGT]CC
NspI 29 ACATGC [GA]CATG^[CT]
PvuII 109 CAGCTG CAG^CTG
SapI 211 GAAGAGC GCTCTTC(1/4)
Sau96I 127 GGGCC G^G[ACGT]CC
SfaNI 281 GCATC GCATC(5/9)
Tsp45I 119 GTGAC ^GT[GC]AC
TspRI 117 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 340 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
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AfaI 102 GTAC GT^AC
240 GTAC
BbvI 111 GCTGC GCAGC(8/12)
195 GCTGC
BfaI 139 CTAG C^TAG
293 CTAG
BsiYI 133 CCGTCCCTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
260 CCATGGAGAGG
BslI 133 CCGTCCCTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
260 CCATGGAGAGG
BsrI 157 ACTGG ACTGG(1/-1)
300 ACTGG
BssKI 150 CCAGG ^CC[ACGT]GG
168 CCAGG
BstNI 150 CCAGG CC^[AT]GG
168 CCAGG
BstXI 77 CCACCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
150 CCAGGGAACTGG
CfoI 25 GCGC GCG^C
276 GCGC
DpnII 228 GATC ^GATC
341 GATC
EaeI 20 TGGCCG [CT]^GGCC[GA]
200 TGGCCA
EcoRII 150 CCAGG ^CC[AT]GG
168 CCAGG
EcoT14I 138 CCTAGG C^C[AT][AT]GG
260 CCATGG
HhaI 25 GCGC GCG^C
276 GCGC
HinP1I 25 GCGC G^CGC
276 GCGC
MaeI 139 CTAG C^TAG
293 CTAG
MaeII 91 ACGT A^CGT
238 ACGT
MboI 228 GATC ^GATC
341 GATC
MboII 211 GAAGA GAAGA(8/7)
94 TCTTC
MspA1I 53 CCGCTG C[AC]G^C[GT]G
109 CAGCTG
MvaI 150 CCAGG CC^[AT]GG
168 CCAGG
NdeII 228 GATC ^GATC
341 GATC
RsaI 102 GTAC GT^AC
240 GTAC
Sau3AI 228 GATC ^GATC
341 GATC
ScrFI 150 CCAGG CC^[ACGT]GG
168 CCAGG
StyI 138 CCTAGG C^C[AT][AT]GG
260 CCATGG
TseI 111 GCTGC G^C[AT]GC
195 GCTGC
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# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
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AccII AciI AluI BsaJI BstUI
Cac8I Fnu4HI HaeIII ItaI MnlI
MvnI NlaIII