(Circular) (Six-base) MAPSORT of: pFN21AE4564.seq Check: 2304 from: 1 to: 6811 pFN21AE4564 6811 bp With 182 enzymes: SgfI * January 12, 2017 12:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2809 3343 2809 Size: 534 6277 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4621 278 Size: 53 83 186 936 3085 2468 Fragments arranged by size: 3085 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2424 4154 1937 Size: 487 1730 4594 Fragments arranged by size: 4594 1730 487 Acc65I G'GTAC_C Cuts at: 4132 4132 Size: 6811 AclI AA'CG_TT Cuts at: 1563 4938 5311 1563 Size: 3375 373 3063 Fragments arranged by size: 3375 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4874 6258 6526 1632 Size: 3242 1384 268 1917 Fragments arranged by size: 3242 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6614 AflIII A'CryG_T Cuts at: 1203 2371 5710 6687 1203 Size: 1168 3339 977 1327 Fragments arranged by size: 3339 1327 1168 977 AgeI A'CCGG_T Cuts at: 5642 5642 Size: 6811 AhdI GACnn_n'nnGTC Cuts at: 5540 5540 Size: 6811 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6811 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6811 AlwNI CAG_nnn'CTG Cuts at: 2921 3494 4028 4554 6126 2921 Size: 573 534 526 1572 3606 Fragments arranged by size: 3606 1572 573 534 526 ApaI G_GGCC'C Cuts at: 2283 2283 Size: 6811 ApaLI G'TGCA_C Cuts at: 4868 6024 4868 Size: 1156 5655 ApoI r'AATT_y Cuts at: 1493 4120 4346 4498 6607 1493 Size: 2627 226 152 2109 1697 Fragments arranged by size: 2627 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5365 160 Size: 872 4333 1606 Fragments arranged by size: 4333 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6811 AvaI C'yCGr_G Cuts at: 1870 1943 4136 1870 Size: 73 2193 4545 Fragments arranged by size: 4545 2193 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2197 2230 2197 Size: 33 6778 BamHI G'GATC_C Cuts at: 2888 4141 4508 2888 Size: 1253 367 5191 Fragments arranged by size: 5191 1253 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4132 5587 618 Size: 325 217 627 2345 1455 1842 Fragments arranged by size: 2345 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2283 2856 3390 4130 1391 Size: 892 573 534 740 4072 Fragments arranged by size: 4072 892 740 573 534 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5840 BbvCI CC'TCA_GC Cuts at: 3302 3302 Size: 6811 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5002 5036 1903 Size: 34 3065 34 3678 Fragments arranged by size: 3678 3065 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3891 4703 5913 3891 Size: 812 1210 4789 Fragments arranged by size: 4789 1210 812 BclI T'GATC_A Cuts at: 1548 1548 Size: 6811 BfrBI ATG'CAT Cuts at: 2318 2318 Size: 6811 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5422 136 Size: 107 122 71 631 605 3750 1525 Fragments arranged by size: 3750 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2386 6806 2386 Size: 4420 2391 BlpI GC'TnA_GC Cuts at: 4228 4228 Size: 6811 Bme1580I G_kGCm'C Cuts at: 1790 2283 4872 6028 1790 Size: 493 2589 1156 2573 Fragments arranged by size: 2589 2573 1156 493 BmgBI CAC'GTC Cuts at: 1318 2374 1318 Size: 1056 5755 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2657 3215 3623 Size: 416 53 83 399 24 1240 558 408 Cuts at: 3623 3749 4542 5500 442 Size: 126 793 958 1753 Fragments arranged by size: 1753 1240 958 793 558 416 408 399 126 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6811 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2032 3851 2032 Size: 1819 4992 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2437 3505 5471 1239 Size: 1198 1068 1966 2579 Fragments arranged by size: 2579 1966 1198 1068 Bpu10I CC'TnA_GC Cuts at: 3302 3302 Size: 6811 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4936 5801 6099 4936 Size: 865 298 5648 Fragments arranged by size: 5648 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4055 5474 882 Size: 617 2556 1419 2219 Fragments arranged by size: 2556 2219 1419 617 BsaAI yAC'GTr Cuts at: 493 1204 2898 4602 493 Size: 711 1694 1704 2702 Fragments arranged by size: 2702 1704 1694 711 BsaBI GATnn'nnATC Cuts at: 6805 6805 Size: 6811 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4618 5000 5635 275 Size: 53 83 186 936 3085 382 635 1451 Fragments arranged by size: 3085 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5244 5642 5916 6063 6474 5244 Size: 398 274 147 411 5581 Fragments arranged by size: 5581 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4077 4107 1178 Size: 30 165 30 2674 30 3882 Fragments arranged by size: 3882 2674 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6811 BseYI C'CCAG_C Cuts at: 2181 4058 6014 6510 2181 Size: 1877 1956 496 2482 Fragments arranged by size: 2482 1956 1877 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2787 3321 1163 Size: 573 1051 534 4653 Fragments arranged by size: 4653 1051 573 534 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4261 5022 6050 1540 Size: 275 186 2260 761 1028 2301 Fragments arranged by size: 2301 2260 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2856 3390 4872 4957 6028 2856 Size: 534 1482 85 1071 3639 Fragments arranged by size: 3639 1482 1071 534 85 BsmI GAATG_Cn' Cuts at: 4322 4415 4322 Size: 93 6718 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2283 2856 3390 4130 4872 4957 6028 Size: 399 493 573 534 740 742 85 1071 Cuts at: 6028 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 740 573 534 493 399 85 BspEI T'CCGG_A Cuts at: 6474 6474 Size: 6811 BspHI T'CATG_A Cuts at: 4077 4700 6430 4077 Size: 623 1730 4458 Fragments arranged by size: 4458 1730 623 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2809 3343 3440 4166 844 Size: 1965 534 97 726 3489 Fragments arranged by size: 3489 1965 726 534 97 BsrBI CCG'CTC Cuts at: 1394 1823 4698 1394 Size: 429 2875 3507 Fragments arranged by size: 3507 2875 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5306 5480 65 Size: 1183 4058 174 1396 Fragments arranged by size: 4058 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5455 5642 1700 Size: 110 3645 187 2869 Fragments arranged by size: 3645 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6811 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6811 BssSI C'ACGA_G Cuts at: 4871 5883 4871 Size: 1012 5799 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6811 BstBI TT'CG_AA Cuts at: 5615 5615 Size: 6811 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6811 BstXI CCAn_nnnn'nTGG Cuts at: 2473 3007 3541 2473 Size: 534 534 5743 Fragments arranged by size: 5743 534 534 BstYI r'GATC_y Cuts at: 1972 2386 2888 4141 4508 4893 4910 6362 6448 Size: 414 502 1253 367 385 17 1452 86 Cuts at: 6448 6460 6806 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1253 502 414 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2425 2425 Size: 6811 Bsu36I CC'TnA_GG Cuts at: 3579 3579 Size: 6811 BtgI C'CryG_G Cuts at: 513 1065 2004 2472 3006 3540 513 Size: 552 939 468 534 534 3784 Fragments arranged by size: 3784 939 552 534 534 468 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6072 Fragments arranged by size: 6072 683 56 BtsI GCAGTG_nn' Cuts at: 2100 2686 3754 4327 5121 5141 2100 Size: 586 1068 573 794 20 3770 Fragments arranged by size: 3770 1068 794 586 573 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6811 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6776 DraI TTT'AAA Cuts at: 4115 4115 Size: 6811 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5818 809 Size: 507 4502 1802 Fragments arranged by size: 4502 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2410 3457 3991 Size: 54 1425 50 275 18 580 1047 534 Cuts at: 3991 4258 5147 6776 8 Size: 267 889 1629 43 Fragments arranged by size: 1629 1425 1047 889 580 534 275 267 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4258 1537 Size: 275 2446 4090 Fragments arranged by size: 4090 2446 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4741 1353 Size: 3388 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5398 5772 5918 387 Size: 1064 138 3809 374 146 1280 Fragments arranged by size: 3809 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2854 3388 2854 Size: 534 6277 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2437 3505 4874 5471 6258 6526 1239 Size: 393 805 1068 1369 597 787 268 1524 Fragments arranged by size: 1524 1369 1068 805 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2050 2050 Size: 6811 EcoO109I rG'GnC_Cy Cuts at: 2279 2598 3132 3170 3666 3957 2279 Size: 319 534 38 496 291 5133 Fragments arranged by size: 5133 534 496 319 291 38 EcoRI G'AATT_C Cuts at: 4120 4498 4120 Size: 378 6433 FspI TGC'GCA Cuts at: 5317 5317 Size: 6811 HaeII r_GCGC'y Cuts at: 1678 5958 1678 Size: 4280 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2025 2057 2590 2622 3124 Size: 32 135 32 453 32 533 32 502 Cuts at: 3124 3156 3658 3690 5452 5484 5526 5558 1373 Size: 32 502 32 1762 32 42 32 2626 Fragments arranged by size: 2626 1762 533 502 502 453 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3208 4155 4401 669 Size: 988 281 1270 947 246 3079 Fragments arranged by size: 3079 1270 988 947 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6811 HpaI GTT'AAC Cuts at: 4401 4401 Size: 6811 KpnI G_GTAC'C Cuts at: 4136 4136 Size: 6811 MfeI C'AATT_G Cuts at: 4410 4410 Size: 6811 MluI A'CGCG_T Cuts at: 6687 6687 Size: 6811 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3024 3423 5925 6109 1305 Size: 1719 399 2502 184 2007 Fragments arranged by size: 2502 2007 1719 399 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2412 3459 10 Size: 54 1425 343 580 1047 3362 Fragments arranged by size: 3362 1425 1047 580 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2873 4769 5128 5287 518 Size: 648 1707 1896 359 159 2042 Fragments arranged by size: 2042 1896 1707 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2202 2581 3115 3420 3649 4058 4227 4904 Size: 596 379 534 305 229 409 169 677 Cuts at: 4904 6052 6297 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 596 534 409 379 305 245 229 169 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5320 Fragments arranged by size: 5320 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6811 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6811 NotI GC'GGCC_GC Cuts at: 4258 4258 Size: 6811 NsiI A_TGCA'T Cuts at: 2320 2320 Size: 6811 NspI r_CATG'y Cuts at: 1132 1229 4169 5714 1132 Size: 97 2940 1545 2229 Fragments arranged by size: 2940 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2276 1348 Size: 928 5883 PciI A'CATG_T Cuts at: 5710 5710 Size: 6811 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6811 PmeI GTTT'AAAC Cuts at: 4115 4115 Size: 6811 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4880 4912 6393 6425 4880 Size: 32 1481 32 5266 Fragments arranged by size: 5266 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2598 3132 3170 3666 3957 2598 Size: 534 38 496 291 5452 Fragments arranged by size: 5452 534 496 291 38 PshAI GACnn'nnGTC Cuts at: 1613 2939 3473 1613 Size: 1326 534 4951 Fragments arranged by size: 4951 1326 534 PsiI TTA'TAA Cuts at: 4381 4381 Size: 6811 PspOMI G'GGCC_C Cuts at: 2279 2279 Size: 6811 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3047 3079 3581 3613 3047 Size: 32 502 32 6245 Fragments arranged by size: 6245 502 32 32 PstI C_TGCA'G Cuts at: 830 2580 3114 3648 4163 830 Size: 1750 534 534 515 3478 Fragments arranged by size: 3478 1750 534 534 515 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6811 PvuII CAG'CTG Cuts at: 2202 2581 3115 3649 4058 2202 Size: 379 534 534 409 4955 Fragments arranged by size: 4955 534 534 409 379 SacI G_AGCT'C Cuts at: 2856 3390 2856 Size: 534 6277 SalI G'TCGA_C Cuts at: 1936 4153 1936 Size: 2217 4594 SbfI CC_TGCA'GG Cuts at: 4163 4163 Size: 6811 ScaI AGT'ACT Cuts at: 2810 5059 6771 2810 Size: 2249 1712 2850 Fragments arranged by size: 2850 2249 1712 SfcI C'TryA_G Cuts at: 826 1045 2197 2576 3024 3110 3471 3644 4159 Size: 219 1152 379 448 86 361 173 515 Cuts at: 4159 5294 5975 6166 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1152 1135 681 515 448 379 361 219 191 173 86 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6811 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6811 SmaI CCC'GGG Cuts at: 1872 4138 1872 Size: 2266 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4915 5816 6078 820 Size: 197 926 2972 901 262 1553 Fragments arranged by size: 2972 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4602 493 Size: 4109 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6811 SphI G_CATG'C Cuts at: 1132 4169 1132 Size: 3037 3774 SspI AAT'ATT Cuts at: 5 52 4735 5 Size: 47 4683 2081 Fragments arranged by size: 4683 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6811 StyI C'CwwG_G Cuts at: 513 1065 2004 4250 513 Size: 552 939 2246 3074 Fragments arranged by size: 3074 2246 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2861 2986 4838 4855 5008 5193 5638 Size: 1255 1281 125 1852 17 153 185 445 Cuts at: 5638 325 Size: 1498 Fragments arranged by size: 1852 1498 1281 1255 445 185 153 125 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2808 5057 6769 Size: 274 80 33 51 1443 831 2249 1712 Cuts at: 6769 96 Size: 138 Fragments arranged by size: 2249 1712 1443 831 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6771 6803 6771 Size: 32 6779 Tth111I GACn'n_nGTC Cuts at: 1316 3221 3755 1316 Size: 1905 534 4372 Fragments arranged by size: 4372 1905 534 XbaI T'CTAG_A Cuts at: 4147 4147 Size: 6811 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2189 2189 Size: 6811 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6811 XmaI C'CCGG_G Cuts at: 1870 4136 1870 Size: 2266 4545 XmnI GAAnn'nnTTC Cuts at: 4940 4940 Size: 6811 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4619 276 Size: 53 83 186 936 3085 2468 Fragments arranged by size: 3085 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BsiWI BsmBI DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI