################################## # NO CUT ENZYMES ( 210 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaJI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoRV EcoT14I EcoT22I FbaI Fnu4HI FokI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI ItaI KasI KpnI KspI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MspA1I MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIII NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI ScrFI SexAI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I TseI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AcsI 274 GAATTC [GA]^AATT[CT] ApoI 274 GAATTC [GA]^AATT[CT] AvaI 233 CCCGAG C^[CT]CG[GA]G BfaI 169 CTAG C^TAG BpmI 206 CTCCAG CTGGAG(16/14) BsaI 97 GAGACC GGTCTC(1/5) BsmAI 97 GAGAC GTCTC(1/5) BsoBI 233 CCCGAG C^[CT]CG[GA]G BspMI 126 ACCTGC ACCTGC(4/8) BstXI 164 CCATACTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 23 GCAGGC GC[ACGT]^[ACGT]GC Cfr13I 3 GGGCC G^G[ACGT]CC EarI 30 GAAGAG CTCTTC(1/4) EcoRI 274 GAATTC G^AATTC FauI 120 CCCGC CCCGC(4/6) HapII 94 CCGG C^CGG HgaI 38 GCGTC GACGC(5/10) HpaII 94 CCGG C^CGG HphI 219 GGTGA GGTGA(8/7) Ksp632I 30 GAAGAG CTCTTC(1/4) MaeI 169 CTAG C^TAG MaeII 191 ACGT A^CGT MboII 30 GAAGA GAAGA(8/7) MslI 165 CATACTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 94 CCGG C^CGG Sau96I 3 GGGCC G^G[ACGT]CC SfaNI 90 GATGC GCATC(5/9) SfcI 180 CTACAG C^T[GA][CT]AG SpeI 168 ACTAGT A^CTAGT TaqI 160 TCGA T^CGA Tsp509I 275 AATT ^AATT TspRI 15 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 160 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AciI 21 CCGC CCGC(-3/-1) 121 CCGC BglI 61 GCCAACGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 70 GCCAATGAGGC BsmFI 110 GGGAC GGGAC(10/14) 40 GTCCC DdeI 114 CTCAG C^T[ACGT]AG 152 CTGAG MwoI 61 GCCAACGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 70 GCCAATGAGGC PleI 112 GACTC GAGTC(4/5) 154 GAGTC SfiI 60 GGCCAACGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 69 GGCCAATGAGGCC TfiI 49 GAATC G^A[AT]TC 245 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### BseRI BsiYI BslI BsrI HaeIII HinfI MnlI