################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI HaeII HindIII HpaI KasI KpnI Ksp632I KspI MamI MflI MluI MluNI MroI MscI MvnI NaeI NarI NcoI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 7 GACGTC GACGT^C AcsI 377 GAATTT [GA]^AATT[CT] AcyI 7 GACGTC G[GA]^CG[CT]C AlwI 840 GATCC GGATC(4/5) AlwNI 670 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 377 GAATTT [GA]^AATT[CT] AviII 616 TGCGCA TGC^GCA BanI 865 GGTGCC G^G[CT][GA]CC BclI 155 TGATCA T^GATCA BcnI 843 CCCGG CC^[GC]GG BglI 55 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 7 GACGTC G[GA]^CG[CT]C BsaI 532 GGTCTC GGTCTC(1/5) BsaWI 438 ACCGGA [AT]^CCGG[AT] BseRI 291 GAGGAG GAGGAG(10/8) BsmI 398 GAATGC GAATGC(1/-1) BsrBI 277 GAGCGG CCGCTC(-3/-3) CfoI 617 GCGC GCG^C DraII 629 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 892 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 629 AGGCCCT [GA]G^G[ACGT]CC[CT] FauI 823 GCGGG CCCGC(4/6) FbaI 155 TGATCA T^GATCA FspI 616 TGCGCA TGC^GCA HgaI 240 GACGC GACGC(5/10) HhaI 617 GCGC GCG^C Hin1I 7 GACGTC G[GA]^CG[CT]C HinP1I 617 GCGC G^CGC HincII 385 GTCAAC GT[CT]^[GA]AC HindII 385 GTCAAC GT[CT]^[GA]AC MfeI 779 CAATTG C^AATTG MseI 347 TTAA T^TAA MslI 666 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 861 CAGCGG C[AC]G^C[GT]G MunI 779 CAATTG C^AATTG NciI 843 CCCGG CC^[GC]GG NheI 201 GCTAGC G^CTAGC NlaIV 865 GGTGCC GG[ACGT]^[ACGT]CC Tru9I 347 TTAA T^TAA Tsp45I 775 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 189 GTAC GT^AC 591 GTAC BfaI 202 CTAG C^TAG 253 CTAG BpmI 81 CTCCAG CTGGAG(16/14) 161 CTGGAG BsmAI 533 GTCTC GTCTC(1/5) 691 GTCTC BsmFI 5 GGGAC GGGAC(10/14) 141 GGGAC BsrDI 261 CATTGC GCAATG(2/0) 700 GCAATG BsrI 104 ACTGG ACTGG(1/-1) 160 ACTGG Cfr13I 630 GGCCC G^G[ACGT]CC 851 GGGCC EcoNI 633 CCTGCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 869 CCTTCCAAAGG HphI 787 GGTGA GGTGA(8/7) 473 TCACC MaeI 202 CTAG C^TAG 253 CTAG MaeII 8 ACGT A^CGT 210 ACGT MaeIII 467 GTAAC ^GT[ACGT]AC 775 GTCAC NdeI 511 CATATG CA^TATG 683 CATATG NlaIII 588 CATG CATG^ 811 CATG PleI 215 GACTC GAGTC(4/5) 760 GAGTC RsaI 189 GTAC GT^AC 591 GTAC Sau96I 630 GGCCC G^G[ACGT]CC 851 GGGCC SfcI 284 CTACAG C^T[GA][CT]AG 858 CTACAG TfiI 77 GATTC G^A[AT]TC 517 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AluI BbvI BsaJI BsiYI BslI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MboI MboII MnlI MspI MvaI MwoI NdeII Sau3AI ScrFI StyI TaqI TseI Tsp509I TspRI TthHB8I