################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AccIII AflII AgeI AocI ApaI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BbeI BbrPI BciVI BclI BfrI BlnI BsaAI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI EagI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV FbaI FseI FspI HpaI KasI KpnI KspI MluI MroI NarI NdeI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI RsrII SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccII 1872 CGCG CG^CG AclI 1334 AACGTT AA^CGTT AcsI 3337 GAATTT [GA]^AATT[CT] AflIII 1796 ACGTGT A^C[GA][CT]GT AhdI 2144 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3272 GTGCAC G^TGCAC ApaLI 3272 GTGCAC G^TGCAC ApoI 3337 GAATTT [GA]^AATT[CT] AspEI 2144 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 589 GGTGCC G^G[CT][GA]CC BglI 1854 GCCCAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 3452 GCTTAGC GC^T[ACGT]AGC Bpu1102I 3452 GCTTAGC GC^T[ACGT]AGC BsaWI 3089 ACCGGA [AT]^CCGG[AT] BspMI 1844 ACCTGC ACCTGC(4/8) BsrBI 406 GAGCGG CCGCTC(-3/-3) BstUI 1872 CGCG CG^CG CelII 3452 GCTTAGC GC^T[ACGT]AGC DraII 39 AGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 995 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2832 GACCAGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2144 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 46 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 39 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoT22I 1066 ATGCAT ATGCA^T HindIII 3396 AAGCTT A^AGCTT MfeI 3185 CAATTG C^AATTG MunI 3185 CAATTG C^AATTG MvnI 1872 CGCG CG^CG NaeI 1673 GCCGGC GCC^GGC NgoMIV 1673 GCCGGC G^CCGGC NheI 2068 GCTAGC G^CTAGC NsiI 1066 ATGCAT ATGCA^T PflMI 1188 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 1334 AACGTT AA^CGTT PstI 1288 CTGCAG CTGCA^G SalI 3025 GTCGAC G^TCGAC SmlI 170 CTCAAG C^T[CT][GA]AG Van91I 1188 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 2757 CCAGCGTGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 2965 GTAGAC GT^[AC][GT]AC 3025 GTCGAC AlwNI 1138 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG 1343 CAGACACTG Aor51HI 1106 AGCGCT AGC^GCT 1200 AGCGCT AvaI 2129 CTCGGG C^[CT]CG[GA]G 2235 CCCGAG BalI 1620 TGGCCA TGG^CCA 2807 TGGCCA BamHI 2111 GGATCC G^GATCC 3176 GGATCC BbsI 739 GAAGAC GAAGAC(2/6) 3510 GTCTTC BbvCI 1830 CCTCAGC CCTCAGC(-5/-2) 2799 GCTGAGG BcgI 1782 CGAGAGTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3264 CGACTTCAGTGC BglII 709 AGATCT A^GATCT 876 AGATCT BpuAI 739 GAAGAC GAAGAC(2/6) 3510 GTCTTC BsaBI 1486 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2766 GATTGGCATC BsaI 120 GGTCTC GGTCTC(1/5) 410 GGTCTC BsmBI 1156 GAGACG CGTCTC(1/5) 2500 GAGACG BsmI 385 GAATGC GAATGC(1/-1) 1777 GCATTC BsoBI 2129 CTCGGG C^[CT]CG[GA]G 2235 CCCGAG BspHI 216 TCATGA T^CATGA 2153 TCATGA BsrDI 3155 GCAATG GCAATG(2/0) 3551 GCAATG BsrFI 1673 GCCGGC [GA]^CCGG[CT] 2255 ACCGGC BstAPI 1125 GCAAAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2583 GCATGAATTGC BstXI 464 CCAGCGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2972 CCAGGATGCTGG Cfr10I 1673 GCCGGC [GA]^CCGG[CT] 2255 ACCGGC Eco47III 1106 AGCGCT AGC^GCT 1200 AGCGCT MamI 1486 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2766 GATTGGCATC MluNI 1620 TGGCCA TGG^CCA 2807 TGGCCA MscI 1620 TGGCCA TGG^CCA 2807 TGGCCA MslI 1008 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3410 CACCTTCATG PvuII 1903 CAGCTG CAG^CTG 3013 CAGCTG RcaI 216 TCATGA T^CATGA 2153 TCATGA Tsp45I 838 GTGAC ^GT[GC]AC 3496 GTGAC XbaI 2004 TCTAGA T^CTAGA 2857 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AspHI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BssSI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII