(Circular) (Six-base) MAPSORT of: pFN21AE1318.seq Check: 6162 from: 1 to: 8311 pFN21AE1318 8311 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6121 278 Size: 53 83 186 936 4585 2468 Fragments arranged by size: 4585 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4971 5031 5654 1937 Size: 3034 60 623 4594 Fragments arranged by size: 4594 3034 623 60 Acc65I G'GTAC_C Cuts at: 5632 5632 Size: 8311 AclI AA'CG_TT Cuts at: 1563 3340 6438 6811 1563 Size: 1777 3098 373 3063 Fragments arranged by size: 3098 3063 1777 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2516 2916 3105 3240 3884 4820 5257 6374 Size: 884 400 189 135 644 936 437 1117 Cuts at: 6374 7758 8026 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1117 936 884 644 437 400 268 189 135 AfeI AGC'GCT Cuts at: 3113 3207 3113 Size: 94 8217 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8114 AflIII A'CryG_T Cuts at: 1203 3801 7210 8187 1203 Size: 2598 3409 977 1327 Fragments arranged by size: 3409 2598 1327 977 AgeI A'CCGG_T Cuts at: 7142 7142 Size: 8311 AhdI GACnn_n'nnGTC Cuts at: 4154 7040 4154 Size: 2886 5425 AleI CACnn'nnGTG Cuts at: 1166 3017 1166 Size: 1851 6460 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4805 1184 Size: 3621 4690 AlwNI CAG_nnn'CTG Cuts at: 3148 3353 6054 7626 3148 Size: 205 2701 1572 3833 Fragments arranged by size: 3833 2701 1572 205 ApaLI G'TGCA_C Cuts at: 5277 6368 7524 5277 Size: 1091 1156 6064 Fragments arranged by size: 6064 1156 1091 ApoI r'AATT_y Cuts at: 1493 5342 5620 5846 5998 8107 1493 Size: 3849 278 226 152 2109 1697 Fragments arranged by size: 3849 2109 1697 278 226 152 AseI AT'TA_AT Cuts at: 160 1032 6865 160 Size: 872 5833 1606 Fragments arranged by size: 5833 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8311 AvaI C'yCGr_G Cuts at: 1870 1943 4134 4240 5636 1870 Size: 73 2191 106 1396 4545 Fragments arranged by size: 4545 2191 1396 106 73 BamHI G'GATC_C Cuts at: 4116 5181 5641 6008 4116 Size: 1065 460 367 6419 Fragments arranged by size: 6419 1065 460 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2594 5632 7087 618 Size: 325 217 627 807 3038 1455 1842 Fragments arranged by size: 3038 1842 1455 807 627 325 217 BanII G_rGCy'C Cuts at: 1391 2769 4043 4249 4671 5630 1391 Size: 1378 1274 206 422 959 4072 Fragments arranged by size: 4072 1378 1274 959 422 206 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2751 5508 928 Size: 971 852 2757 3731 Fragments arranged by size: 3731 2757 971 852 BbvCI CC'TCA_GC Cuts at: 3836 4805 3836 Size: 969 7342 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3776 3810 5258 5292 6502 6536 1903 Size: 34 1839 34 1448 34 1210 34 3678 Fragments arranged by size: 3678 1839 1448 1210 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6203 7413 6203 Size: 1210 7101 BclI T'GATC_A Cuts at: 1548 1548 Size: 8311 BfrBI ATG'CAT Cuts at: 3073 3073 Size: 8311 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3865 6922 136 Size: 107 122 71 631 605 2193 3057 1525 Fragments arranged by size: 3057 2193 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2714 2881 8306 2714 Size: 167 5425 2719 Fragments arranged by size: 5425 2719 167 BlpI GC'TnA_GC Cuts at: 5458 5728 5458 Size: 270 8041 Bme1580I G_kGCm'C Cuts at: 1790 3748 5281 6372 7528 1790 Size: 1958 1533 1091 1156 2573 Fragments arranged by size: 2573 1958 1533 1156 1091 BmgBI CAC'GTC Cuts at: 1318 3802 5146 1318 Size: 2484 1344 4483 Fragments arranged by size: 4483 2484 1344 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4160 4933 6042 Size: 416 53 83 399 24 2743 773 1109 Cuts at: 6042 7000 442 Size: 958 1753 Fragments arranged by size: 2743 1753 1109 958 773 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4077 1056 Size: 3021 5290 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2190 2190 Size: 8311 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2073 2386 2439 6971 1239 Size: 834 313 53 4532 2579 Fragments arranged by size: 4532 2579 834 313 53 Bpu10I CC'TnA_GC Cuts at: 3836 4805 3836 Size: 969 7342 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2160 6436 7301 7599 2160 Size: 4276 865 298 2872 Fragments arranged by size: 4276 2872 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2131 2421 6974 882 Size: 617 632 290 4553 2219 Fragments arranged by size: 4553 2219 632 617 290 BsaAI yAC'GTr Cuts at: 493 1204 6102 493 Size: 711 4898 2702 Fragments arranged by size: 4898 2702 711 BsaBI GATnn'nnATC Cuts at: 3495 4775 8305 3495 Size: 1280 3530 3501 Fragments arranged by size: 3530 3501 1280 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3915 4155 4974 6118 Size: 53 83 186 936 2382 240 819 1144 Cuts at: 6118 6500 7135 275 Size: 382 635 1451 Fragments arranged by size: 2382 1451 1144 936 819 635 382 240 186 83 53 BsaWI w'CCGG_w Cuts at: 5094 6744 7142 7416 7563 7974 5094 Size: 1650 398 274 147 411 5431 Fragments arranged by size: 5431 1650 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3620 3650 4603 4633 4814 Size: 30 165 30 2217 30 953 30 181 Cuts at: 4814 4844 1178 Size: 30 4645 Fragments arranged by size: 4645 2217 953 181 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2070 3290 3824 3992 4069 1186 Size: 884 1220 534 168 77 5428 Fragments arranged by size: 5428 1220 884 534 168 77 BseYI C'CCAG_C Cuts at: 3747 3860 4129 4355 4597 4922 5465 7514 8010 Size: 113 269 226 242 325 543 2049 496 Cuts at: 8010 3747 Size: 4048 Fragments arranged by size: 4048 2049 543 496 325 269 242 226 113 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2982 3672 4250 4265 4775 1163 Size: 573 1246 690 578 15 510 4699 Fragments arranged by size: 4699 1246 690 578 573 510 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5761 6522 7550 1540 Size: 275 186 3760 761 1028 2301 Fragments arranged by size: 3760 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2769 4043 4671 5245 5281 5416 6372 6457 7528 Size: 1274 628 574 36 135 956 85 1071 Cuts at: 7528 2769 Size: 3552 Fragments arranged by size: 3552 1274 1071 956 628 574 135 85 36 BsmI GAATG_Cn' Cuts at: 2396 3782 5822 5915 2396 Size: 1386 2040 93 4792 Fragments arranged by size: 4792 2040 1386 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3155 4499 3155 Size: 1344 6967 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2769 3748 4043 4249 4671 5245 5281 Size: 399 979 979 295 206 422 574 36 Cuts at: 5281 5416 5630 6372 6457 7528 1391 Size: 135 214 742 85 1071 2174 Fragments arranged by size: 2174 1071 979 979 742 574 422 399 295 214 206 135 85 36 BspEI T'CCGG_A Cuts at: 7974 7974 Size: 8311 BspHI T'CATG_A Cuts at: 2221 4158 6200 7930 2221 Size: 1937 2042 1730 2602 Fragments arranged by size: 2602 2042 1937 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3858 5666 844 Size: 3014 1808 3489 Fragments arranged by size: 3489 3014 1808 BsrBI CCG'CTC Cuts at: 1394 1823 2413 6198 1394 Size: 429 590 3785 3507 Fragments arranged by size: 3785 3507 590 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5167 5563 6806 6980 65 Size: 1183 3919 396 1243 174 1396 Fragments arranged by size: 3919 1396 1243 1183 396 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3678 4260 6955 7142 1700 Size: 110 1868 582 2695 187 2869 Fragments arranged by size: 2869 2695 1868 582 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8311 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8311 BssSI C'ACGA_G Cuts at: 2210 2701 2738 4295 4895 5322 6371 7383 2210 Size: 491 37 1557 600 427 1049 1012 3138 Fragments arranged by size: 3138 1557 1049 1012 600 491 427 37 BstAPI GCAn_nnn'nTGC Cuts at: 794 3136 4594 794 Size: 2342 1458 4511 Fragments arranged by size: 4511 2342 1458 BstBI TT'CG_AA Cuts at: 7115 7115 Size: 8311 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8311 BstXI CCAn_nnnn'nTGG Cuts at: 2476 4984 2476 Size: 2508 5803 BstYI r'GATC_y Cuts at: 1972 2714 2860 2881 4116 4758 5181 5298 5641 Size: 742 146 21 1235 642 423 117 343 Cuts at: 5641 6008 6393 6410 7862 7948 7960 8306 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1235 742 642 423 385 367 346 343 146 117 86 21 17 12 BtgI C'CryG_G Cuts at: 513 1065 2068 2430 2937 3615 513 Size: 552 1003 362 507 678 5209 Fragments arranged by size: 5209 1003 678 552 507 362 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7572 Fragments arranged by size: 7572 683 56 BtsI GCAGTG_nn' Cuts at: 3598 3660 4006 4099 5285 5827 6621 6641 3598 Size: 62 346 93 1186 542 794 20 5268 Fragments arranged by size: 5268 1186 794 542 346 93 62 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8311 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4635 4670 529 Size: 35 4071 35 4170 Fragments arranged by size: 4170 4071 35 35 DraI TTT'AAA Cuts at: 5615 5615 Size: 8311 DraIII CAC_nnn'GTG Cuts at: 3005 3005 Size: 8311 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4843 7318 809 Size: 507 3527 2475 1802 Fragments arranged by size: 3527 2475 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3574 3625 4426 Size: 54 1425 50 275 18 1744 51 801 Cuts at: 4426 4812 5758 6647 8276 8 Size: 386 946 889 1629 43 Fragments arranged by size: 1744 1629 1425 946 889 801 386 275 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5758 1537 Size: 275 3946 4090 Fragments arranged by size: 4090 3946 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2432 2577 2850 2955 3216 4179 6241 1353 Size: 1079 145 273 105 261 963 2062 3423 Fragments arranged by size: 3423 2062 1079 963 273 261 145 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3197 6898 7272 7418 387 Size: 1064 138 1608 3701 374 146 1280 Fragments arranged by size: 3701 1608 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2767 4041 4669 2767 Size: 1274 628 6409 Fragments arranged by size: 6409 1274 628 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2073 2386 2439 2516 2916 3105 3240 Size: 393 441 313 53 77 400 189 135 Cuts at: 3240 3884 4820 5257 6374 6971 7758 8026 1239 Size: 644 936 437 1117 597 787 268 1524 Fragments arranged by size: 1524 1117 936 787 644 597 441 437 400 393 313 268 189 135 77 53 EcoNI CCTnn'n_nnAGG Cuts at: 2055 2055 Size: 8311 EcoO109I rG'GnC_Cy Cuts at: 2045 2045 Size: 8311 EcoRI G'AATT_C Cuts at: 5620 5998 5620 Size: 378 7933 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3576 5165 3576 Size: 1589 6722 FspI TGC'GCA Cuts at: 6817 6817 Size: 8311 HaeII r_GCGC'y Cuts at: 1678 3115 3209 4102 4280 7458 1678 Size: 1437 94 893 178 3178 2531 Fragments arranged by size: 3178 2531 1437 893 178 94 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3419 3451 3839 3871 3973 Size: 32 135 32 1847 32 388 32 102 Cuts at: 3973 4005 4140 4172 4502 4534 4814 4846 5149 Size: 32 135 32 330 32 280 32 303 Cuts at: 5149 5181 6952 6984 7026 7058 1373 Size: 32 1771 32 42 32 2626 Fragments arranged by size: 2626 1847 1771 388 330 303 280 135 135 102 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3358 3847 5032 5655 5901 669 Size: 988 281 1420 489 1185 623 246 3079 Fragments arranged by size: 3079 1420 1185 988 623 489 281 246 HindIII A'AGCT_T Cuts at: 748 5401 748 Size: 4653 3658 HpaI GTT'AAC Cuts at: 5901 5901 Size: 8311 KpnI G_GTAC'C Cuts at: 5636 5636 Size: 8311 MfeI C'AATT_G Cuts at: 5190 5910 5190 Size: 720 7591 MluI A'CGCG_T Cuts at: 8187 8187 Size: 8311 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3252 4773 5559 7425 7609 1305 Size: 1947 1521 786 1866 184 2007 Fragments arranged by size: 2007 1947 1866 1521 786 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3627 4814 10 Size: 54 1425 343 1795 1187 3507 Fragments arranged by size: 3507 1795 1425 1187 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3017 5419 6269 6628 6787 518 Size: 648 1851 2402 850 359 159 2042 Fragments arranged by size: 2402 2042 1851 850 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3298 3864 3910 3970 5020 5727 6404 7552 Size: 1692 566 46 60 1050 707 677 1148 Cuts at: 7552 7797 1606 Size: 245 2120 Fragments arranged by size: 2120 1692 1148 1050 707 677 566 245 60 46 NaeI GCC'GGC Cuts at: 3680 3680 Size: 8311 NcoI C'CATG_G Cuts at: 513 1065 2068 2937 3615 513 Size: 552 1003 869 678 5209 Fragments arranged by size: 5209 1003 869 678 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8311 NgoMIV G'CCGG_C Cuts at: 3678 3678 Size: 8311 NheI G'CTAG_C Cuts at: 1052 4073 1052 Size: 3021 5290 NotI GC'GGCC_GC Cuts at: 5758 5758 Size: 8311 NsiI A_TGCA'T Cuts at: 3075 3075 Size: 8311 NspI r_CATG'y Cuts at: 1132 1229 5669 7214 1132 Size: 97 4440 1545 2229 Fragments arranged by size: 4440 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2197 1348 Size: 849 7462 PciI A'CATG_T Cuts at: 7210 7210 Size: 8311 PflMI CCAn_nnn'nTGG Cuts at: 1108 3199 1108 Size: 2091 6220 PfoI T'CCnGG_A Cuts at: 3168 4818 3168 Size: 1650 6661 PmeI GTTT'AAAC Cuts at: 5615 5615 Size: 8311 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6380 6412 7893 7925 6380 Size: 32 1481 32 6766 Fragments arranged by size: 6766 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8311 PsiI TTA'TAA Cuts at: 5881 5881 Size: 8311 PstI C_TGCA'G Cuts at: 830 3297 5663 830 Size: 2467 2366 3478 Fragments arranged by size: 3478 2467 2366 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8311 PvuII CAG'CTG Cuts at: 3910 5020 3910 Size: 1110 7201 SacI G_AGCT'C Cuts at: 2769 4043 4671 2769 Size: 1274 628 6409 Fragments arranged by size: 6409 1274 628 SalI G'TCGA_C Cuts at: 1936 5030 5653 1936 Size: 3094 623 4594 Fragments arranged by size: 4594 3094 623 SbfI CC_TGCA'GG Cuts at: 5663 5663 Size: 8311 ScaI AGT'ACT Cuts at: 6559 8271 6559 Size: 1712 6599 SfcI C'TryA_G Cuts at: 826 1045 3293 3640 5589 5659 6794 7475 7666 Size: 219 2248 347 1949 70 1135 681 191 Cuts at: 7666 826 Size: 1471 Fragments arranged by size: 2248 1949 1471 1135 681 347 219 191 70 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8311 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8311 SmaI CCC'GGG Cuts at: 1872 5638 1872 Size: 3766 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2175 6415 7316 7578 820 Size: 197 926 232 4240 901 262 1553 Fragments arranged by size: 4240 1553 926 901 262 232 197 SnaBI TAC'GTA Cuts at: 493 6102 493 Size: 5609 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8311 SphI G_CATG'C Cuts at: 1132 5669 1132 Size: 4537 3774 SspI AAT'ATT Cuts at: 5 52 6235 5 Size: 47 6183 2081 Fragments arranged by size: 6183 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8311 StyI C'CwwG_G Cuts at: 513 1065 2068 2937 3213 3615 3708 4530 5750 Size: 552 1003 869 276 402 93 822 1220 Cuts at: 5750 513 Size: 3074 Fragments arranged by size: 3074 1220 1003 869 822 552 402 276 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6338 6355 6508 6693 7138 325 Size: 1255 4758 17 153 185 445 1498 Fragments arranged by size: 4758 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6557 8269 96 Size: 274 80 33 51 1443 4580 1712 138 Fragments arranged by size: 4580 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2245 2277 3620 3652 4101 4133 8271 8303 2245 Size: 32 1343 32 449 32 4138 32 2253 Fragments arranged by size: 4138 2253 1343 449 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8311 XbaI T'CTAG_A Cuts at: 4009 4862 5647 4009 Size: 853 785 6673 Fragments arranged by size: 6673 853 785 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4769 4769 Size: 8311 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8311 XmaI C'CCGG_G Cuts at: 1870 5636 1870 Size: 3766 4545 XmnI GAAnn'nnTTC Cuts at: 6440 6440 Size: 8311 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6119 276 Size: 53 83 186 936 4585 2468 Fragments arranged by size: 4585 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI