(Linear) (Six-base) MAPSORT of: KIEE1318.seq Check: 2018 from: 1 to: 3606 KIEE1318.orf With 182 enzymes: SgfI * July 15, 2010 18:36 .. AccI GT'mk_AC Cuts at: 0 2966 3026 3606 Size: 2966 60 580 Fragments arranged by size: 2966 580 60 AclI AA'CG_TT Cuts at: 0 1335 3606 Size: 1335 2271 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 511 911 1100 1235 1879 2815 3252 3606 Size: 511 400 189 135 644 936 437 354 Fragments arranged by size: 936 644 511 437 400 354 189 135 AfeI AGC'GCT Cuts at: 0 1108 1202 3606 Size: 1108 94 2404 Fragments arranged by size: 2404 1108 94 AflIII A'CryG_T Cuts at: 0 1796 3606 Size: 1796 1810 AhdI GACnn_n'nnGTC Cuts at: 0 2149 3606 Size: 2149 1457 AleI CACnn'nnGTG Cuts at: 0 1012 3606 Size: 1012 2594 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2800 3606 Size: 2800 806 AlwNI CAG_nnn'CTG Cuts at: 0 1143 1348 3606 Size: 1143 205 2258 Fragments arranged by size: 2258 1143 205 ApaLI G'TGCA_C Cuts at: 0 3272 3606 Size: 3272 334 ApoI r'AATT_y Cuts at: 0 3337 3606 Size: 3337 269 AvaI C'yCGr_G Cuts at: 0 2129 2235 3606 Size: 2129 106 1371 Fragments arranged by size: 2129 1371 106 BamHI G'GATC_C Cuts at: 0 2111 3176 3606 Size: 2111 1065 430 Fragments arranged by size: 2111 1065 430 BanI G'GyrC_C Cuts at: 0 589 3606 Size: 589 3017 BanII G_rGCy'C Cuts at: 0 764 2038 2244 2666 3606 Size: 764 1274 206 422 940 Fragments arranged by size: 1274 940 764 422 206 BbsI GAAGACnn'nnnn_ Cuts at: 0 746 3503 3606 Size: 746 2757 103 Fragments arranged by size: 2757 746 103 BbvCI CC'TCA_GC Cuts at: 0 1831 2800 3606 Size: 1831 969 806 Fragments arranged by size: 1831 969 806 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1771 1805 3253 3287 3606 Size: 1771 34 1448 34 319 Fragments arranged by size: 1771 1448 319 34 34 BfrBI ATG'CAT Cuts at: 0 1068 3606 Size: 1068 2538 BglI GCCn_nnn'nGGC Cuts at: 0 1860 3606 Size: 1860 1746 BglII A'GATC_T Cuts at: 0 709 876 3606 Size: 709 167 2730 Fragments arranged by size: 2730 709 167 BlpI GC'TnA_GC Cuts at: 0 3453 3606 Size: 3453 153 Bme1580I G_kGCm'C Cuts at: 0 1743 3276 3606 Size: 1743 1533 330 Fragments arranged by size: 1743 1533 330 BmgBI CAC'GTC Cuts at: 0 1797 3141 3606 Size: 1797 1344 465 Fragments arranged by size: 1797 1344 465 BmrI ACTGGGnnnn_n' Cuts at: 0 2155 2928 3606 Size: 2155 773 678 Fragments arranged by size: 2155 773 678 BmtI G_CTAG'C Cuts at: 0 2072 3606 Size: 2072 1534 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 185 3606 Size: 185 3421 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 68 381 434 3606 Size: 68 313 53 3172 Fragments arranged by size: 3172 313 68 53 Bpu10I CC'TnA_GC Cuts at: 0 1831 2800 3606 Size: 1831 969 806 Fragments arranged by size: 1831 969 806 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 155 3606 Size: 155 3451 BsaI GGTCTCn'nnnn_ Cuts at: 0 126 416 3606 Size: 126 290 3190 Fragments arranged by size: 3190 290 126 BsaBI GATnn'nnATC Cuts at: 0 1490 2770 3606 Size: 1490 1280 836 Fragments arranged by size: 1490 1280 836 BsaHI Gr'CG_yC Cuts at: 0 1910 2150 2969 3606 Size: 1910 240 819 637 Fragments arranged by size: 1910 819 637 240 BsaWI w'CCGG_w Cuts at: 0 3089 3606 Size: 3089 517 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1615 1645 2598 2628 2809 2839 3606 Size: 1615 30 953 30 181 30 767 Fragments arranged by size: 1615 953 767 181 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 65 1285 1819 1987 2064 3606 Size: 65 1220 534 168 77 1542 Fragments arranged by size: 1542 1220 534 168 77 65 BseYI C'CCAG_C Cuts at: 0 1742 1855 2124 2350 2592 2917 3460 3606 Size: 1742 113 269 226 242 325 543 146 Fragments arranged by size: 1742 543 325 269 242 226 146 113 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 977 1667 2245 2260 2770 3606 Size: 977 690 578 15 510 836 Fragments arranged by size: 977 836 690 578 510 15 BsiHKAI G_wGCw'C Cuts at: 0 764 2038 2666 3240 3276 3411 3606 Size: 764 1274 628 574 36 135 195 Fragments arranged by size: 1274 764 628 574 195 135 36 BsmI GAATG_Cn' Cuts at: 0 391 1777 3606 Size: 391 1386 1829 Fragments arranged by size: 1829 1386 391 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1150 2494 3606 Size: 1150 1344 1112 Fragments arranged by size: 1344 1150 1112 Bsp1286I G_dGCh'C Cuts at: 0 764 1743 2038 2244 2666 3240 3276 3411 Size: 764 979 295 206 422 574 36 135 Cuts at: 3411 3606 Size: 195 Fragments arranged by size: 979 764 574 422 295 206 195 135 36 BspHI T'CATG_A Cuts at: 0 216 2153 3606 Size: 216 1937 1453 Fragments arranged by size: 1937 1453 216 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1853 3606 Size: 1853 1753 BsrBI CCG'CTC Cuts at: 0 408 3606 Size: 408 3198 BsrDI GCAATG_nn' Cuts at: 0 3162 3558 3606 Size: 3162 396 48 Fragments arranged by size: 3162 396 48 BsrFI r'CCGG_y Cuts at: 0 1673 2255 3606 Size: 1673 582 1351 Fragments arranged by size: 1673 1351 582 BssSI C'ACGA_G Cuts at: 0 205 696 733 2290 2890 3317 3606 Size: 205 491 37 1557 600 427 289 Fragments arranged by size: 1557 600 491 427 289 205 37 BstAPI GCAn_nnn'nTGC Cuts at: 0 1131 2589 3606 Size: 1131 1458 1017 Fragments arranged by size: 1458 1131 1017 BstXI CCAn_nnnn'nTGG Cuts at: 0 471 2979 3606 Size: 471 2508 627 Fragments arranged by size: 2508 627 471 BstYI r'GATC_y Cuts at: 0 709 855 876 2111 2753 3176 3293 3606 Size: 709 146 21 1235 642 423 117 313 Fragments arranged by size: 1235 709 642 423 313 146 117 21 BtgI C'CryG_G Cuts at: 0 63 425 932 1610 3606 Size: 63 362 507 678 1996 Fragments arranged by size: 1996 678 507 362 63 BtsI GCAGTG_nn' Cuts at: 0 1593 1655 2001 2094 3280 3606 Size: 1593 62 346 93 1186 326 Fragments arranged by size: 1593 1186 346 326 93 62 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2630 2665 3606 Size: 2630 35 941 Fragments arranged by size: 2630 941 35 DraIII CAC_nnn'GTG Cuts at: 0 1000 3606 Size: 1000 2606 DrdI GACnn_nn'nnGTC Cuts at: 0 2838 3606 Size: 2838 768 EaeI y'GGCC_r Cuts at: 0 1569 1620 2421 2807 3606 Size: 1569 51 801 386 799 Fragments arranged by size: 1569 801 799 386 51 EarI CTCTTCn'nnn_ Cuts at: 0 427 572 845 950 1211 2174 3606 Size: 427 145 273 105 261 963 1432 Fragments arranged by size: 1432 963 427 273 261 145 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1192 3606 Size: 1192 2414 EcoICRI GAG'CTC Cuts at: 0 762 2036 2664 3606 Size: 762 1274 628 942 Fragments arranged by size: 1274 942 762 628 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 68 381 434 511 911 1100 1235 1879 Size: 68 313 53 77 400 189 135 644 Cuts at: 1879 2815 3252 3606 Size: 936 437 354 Fragments arranged by size: 936 644 437 400 354 313 189 135 77 68 53 EcoNI CCTnn'n_nnAGG Cuts at: 0 50 3606 Size: 50 3556 EcoO109I rG'GnC_Cy Cuts at: 0 40 3606 Size: 40 3566 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1571 3160 3606 Size: 1571 1589 446 Fragments arranged by size: 1589 1571 446 HaeII r_GCGC'y Cuts at: 0 1110 1204 2097 2275 3606 Size: 1110 94 893 178 1331 Fragments arranged by size: 1331 1110 893 178 94 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1414 1446 1834 1866 1968 2000 2135 2167 Size: 1414 32 388 32 102 32 135 32 Cuts at: 2167 2497 2529 2809 2841 3144 3176 3606 Size: 330 32 280 32 303 32 430 Fragments arranged by size: 1414 430 388 330 303 280 135 102 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1353 1842 3027 3606 Size: 1353 489 1185 579 Fragments arranged by size: 1353 1185 579 489 HindIII A'AGCT_T Cuts at: 0 3396 3606 Size: 3396 210 MfeI C'AATT_G Cuts at: 0 3185 3606 Size: 3185 421 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1247 2768 3554 3606 Size: 1247 1521 786 52 Fragments arranged by size: 1521 1247 786 52 MscI TGG'CCA Cuts at: 0 1622 2809 3606 Size: 1622 1187 797 Fragments arranged by size: 1622 1187 797 MslI CAynn'nnrTG Cuts at: 0 1012 3414 3606 Size: 1012 2402 192 Fragments arranged by size: 2402 1012 192 MspA1I CmG'CkG Cuts at: 0 1293 1859 1905 1965 3015 3606 Size: 1293 566 46 60 1050 591 Fragments arranged by size: 1293 1050 591 566 60 46 NaeI GCC'GGC Cuts at: 0 1675 3606 Size: 1675 1931 NcoI C'CATG_G Cuts at: 0 63 932 1610 3606 Size: 63 869 678 1996 Fragments arranged by size: 1996 869 678 63 NgoMIV G'CCGG_C Cuts at: 0 1673 3606 Size: 1673 1933 NheI G'CTAG_C Cuts at: 0 2068 3606 Size: 2068 1538 NsiI A_TGCA'T Cuts at: 0 1070 3606 Size: 1070 2536 PasI CC'CwG_GG Cuts at: 0 192 3606 Size: 192 3414 PflMI CCAn_nnn'nTGG Cuts at: 0 1194 3606 Size: 1194 2412 PfoI T'CCnGG_A Cuts at: 0 1163 2813 3606 Size: 1163 1650 793 Fragments arranged by size: 1650 1163 793 PstI C_TGCA'G Cuts at: 0 1292 3606 Size: 1292 2314 PvuII CAG'CTG Cuts at: 0 1905 3015 3606 Size: 1905 1110 591 Fragments arranged by size: 1905 1110 591 SacI G_AGCT'C Cuts at: 0 764 2038 2666 3606 Size: 764 1274 628 940 Fragments arranged by size: 1274 940 764 628 SalI G'TCGA_C Cuts at: 0 3025 3606 Size: 3025 581 SfcI C'TryA_G Cuts at: 0 1288 1635 3584 3606 Size: 1288 347 1949 22 Fragments arranged by size: 1949 1288 347 22 SmlI C'TyrA_G Cuts at: 0 170 3606 Size: 170 3436 StyI C'CwwG_G Cuts at: 0 63 932 1208 1610 1703 2525 3606 Size: 63 869 276 402 93 822 1081 Fragments arranged by size: 1081 869 822 402 276 93 63 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 240 272 1615 1647 2096 2128 3606 Size: 240 32 1343 32 449 32 1478 Fragments arranged by size: 1478 1343 449 240 32 32 32 XbaI T'CTAG_A Cuts at: 0 2004 2857 3606 Size: 2004 853 749 Fragments arranged by size: 2004 853 749 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2764 3606 Size: 2764 842 Enzymes that do cut: AccI AclI AcuI AfeI AflIII AhdI AleI AlfI AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI BtgI BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NheI NsiI PasI PflMI PfoI PstI PvuII SacI SalI SfcI SmlI StyI TstI XbaI XcmI Enzymes that do not cut: AarI AatII Acc65I AflII AgeI AloI ApaI AscI AseI AsiSI AvrII BaeI BbeI BciVI BclI BsaAI BsiEI BsiWI BspEI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI EagI EcoRI EcoRV FseI FspI FspAI HpaI KasI KpnI MluI NarI NdeI NotI NruI NspI PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XhoI XmaI XmnI ZraI