(Circular) (Six-base) MAPSORT of: pFN21AE0974.seq Check: 1523 from: 1 to: 6286 pFN21AE0974 6286 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2030 2082 2030 Size: 52 6234 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4096 278 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2200 2353 3629 1937 Size: 263 153 1276 4594 Fragments arranged by size: 4594 1276 263 153 Acc65I G'GTAC_C Cuts at: 3607 3607 Size: 6286 AclI AA'CG_TT Cuts at: 1563 4413 4786 1563 Size: 2850 373 3063 Fragments arranged by size: 3063 2850 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2268 2435 2754 2924 4349 5733 6001 1632 Size: 636 167 319 170 1425 1384 268 1917 Fragments arranged by size: 1917 1425 1384 636 319 268 170 167 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6089 AflIII A'CryG_T Cuts at: 1203 2027 3403 5185 6162 1203 Size: 824 1376 1782 977 1327 Fragments arranged by size: 1782 1376 1327 977 824 AgeI A'CCGG_T Cuts at: 5117 5117 Size: 6286 AhdI GACnn_n'nnGTC Cuts at: 5015 5015 Size: 6286 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6286 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6286 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3055 3087 3055 Size: 32 6254 AlwNI CAG_nnn'CTG Cuts at: 2349 2365 2761 4029 5601 2349 Size: 16 396 1268 1572 3034 Fragments arranged by size: 3034 1572 1268 396 16 ApaI G_GGCC'C Cuts at: 3568 3568 Size: 6286 ApaLI G'TGCA_C Cuts at: 3080 4343 5499 3080 Size: 1263 1156 3867 Fragments arranged by size: 3867 1263 1156 ApoI r'AATT_y Cuts at: 1493 3595 3821 3973 6082 1493 Size: 2102 226 152 2109 1697 Fragments arranged by size: 2109 2102 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4840 160 Size: 872 3808 1606 Fragments arranged by size: 3808 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 AvaI C'yCGr_G Cuts at: 1870 1943 2160 3507 3611 1870 Size: 73 217 1347 104 4545 Fragments arranged by size: 4545 1347 217 104 73 BamHI G'GATC_C Cuts at: 3616 3983 3616 Size: 367 5919 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2453 2531 2909 3516 3607 Size: 325 217 627 666 78 378 607 91 Cuts at: 3607 5062 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 666 627 607 378 325 217 91 78 BanII G_rGCy'C Cuts at: 1391 2966 3140 3568 3605 1391 Size: 1575 174 428 37 4072 Fragments arranged by size: 4072 1575 428 174 37 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2310 928 Size: 971 411 4904 Fragments arranged by size: 4904 971 411 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4477 4511 1903 Size: 34 2540 34 3678 Fragments arranged by size: 3678 2540 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4178 5388 4178 Size: 1210 5076 BclI T'GATC_A Cuts at: 1548 2748 1548 Size: 1200 5086 BfrBI ATG'CAT Cuts at: 2851 2851 Size: 6286 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3002 4897 136 Size: 107 122 71 631 605 1330 1895 1525 Fragments arranged by size: 1895 1525 1330 631 605 122 107 71 BglII A'GATC_T Cuts at: 6281 6281 Size: 6286 BlpI GC'TnA_GC Cuts at: 3703 3703 Size: 6286 Bme1580I G_kGCm'C Cuts at: 1790 3084 3568 4347 5503 1790 Size: 1294 484 779 1156 2573 Fragments arranged by size: 2573 1294 1156 779 484 BmgBI CAC'GTC Cuts at: 1318 3406 1318 Size: 2088 4198 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3451 4017 4975 Size: 416 53 83 399 24 2034 566 958 Cuts at: 4975 442 Size: 1753 Fragments arranged by size: 2034 1753 958 566 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6286 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2387 2709 2920 4946 1239 Size: 1148 322 211 2026 2579 Fragments arranged by size: 2579 2026 1148 322 211 Bpu10I CC'TnA_GC Cuts at: 3559 3559 Size: 6286 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2581 2789 4411 5276 5574 2581 Size: 208 1622 865 298 3293 Fragments arranged by size: 3293 1622 865 298 208 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3497 4949 882 Size: 617 1998 1452 2219 Fragments arranged by size: 2219 1998 1452 617 BsaAI yAC'GTr Cuts at: 493 1204 3412 4077 493 Size: 711 2208 665 2702 Fragments arranged by size: 2702 2208 711 665 BsaBI GATnn'nnATC Cuts at: 2655 6280 2655 Size: 3625 2661 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4093 4475 5110 275 Size: 53 83 186 936 2560 382 635 1451 Fragments arranged by size: 2560 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4719 5117 5391 5538 5949 4719 Size: 398 274 147 411 5056 Fragments arranged by size: 5056 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2713 2743 1178 Size: 30 165 30 1310 30 4721 Fragments arranged by size: 4721 1310 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 1186 Size: 886 5400 BseYI C'CCAG_C Cuts at: 2773 5489 5985 2773 Size: 2716 496 3074 Fragments arranged by size: 3074 2716 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2008 1163 Size: 573 272 5441 Fragments arranged by size: 5441 573 272 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3736 4497 5525 1540 Size: 275 186 1735 761 1028 2301 Fragments arranged by size: 2301 1735 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2966 3084 4347 4432 5503 2966 Size: 118 1263 85 1071 3749 Fragments arranged by size: 3749 1263 1071 118 85 BsmI GAATG_Cn' Cuts at: 3797 3890 3797 Size: 93 6193 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2966 3084 3140 3568 3605 4347 4432 Size: 399 1176 118 56 428 37 742 85 Cuts at: 4432 5503 1391 Size: 1071 2174 Fragments arranged by size: 2174 1176 1071 742 428 399 118 85 56 37 BspEI T'CCGG_A Cuts at: 5949 5949 Size: 6286 BspHI T'CATG_A Cuts at: 4175 5905 4175 Size: 1730 4556 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2030 2082 2436 3574 3641 844 Size: 1186 52 354 1138 67 3489 Fragments arranged by size: 3489 1186 1138 354 67 52 BsrBI CCG'CTC Cuts at: 1394 1823 4173 1394 Size: 429 2350 3507 Fragments arranged by size: 3507 2350 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4781 4955 65 Size: 1183 3533 174 1396 Fragments arranged by size: 3533 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2285 2996 4930 5117 1700 Size: 110 475 711 1934 187 2869 Fragments arranged by size: 2869 1934 711 475 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6286 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6286 BssSI C'ACGA_G Cuts at: 2267 3314 4346 5358 2267 Size: 1047 1032 1012 3195 Fragments arranged by size: 3195 1047 1032 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6286 BstBI TT'CG_AA Cuts at: 5090 5090 Size: 6286 BstEII G'GTnAC_C Cuts at: 1184 3308 1184 Size: 2124 4162 BstXI CCAn_nnnn'nTGG Cuts at: 3416 3416 Size: 6286 BstYI r'GATC_y Cuts at: 1972 2569 3616 3983 4368 4385 5837 5923 5935 Size: 597 1047 367 385 17 1452 86 12 Cuts at: 5935 6281 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1047 597 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2354 2354 Size: 6286 Bsu36I CC'TnA_GG Cuts at: 2553 3297 2553 Size: 744 5542 BtgI C'CryG_G Cuts at: 513 1065 2004 2813 2832 513 Size: 552 939 809 19 3967 Fragments arranged by size: 3967 939 809 552 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3421 487 Size: 683 56 2195 3352 Fragments arranged by size: 3352 2195 683 56 BtsI GCAGTG_nn' Cuts at: 3553 3802 4596 4616 3553 Size: 249 794 20 5223 Fragments arranged by size: 5223 794 249 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6286 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6251 DraI TTT'AAA Cuts at: 3590 3590 Size: 6286 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5293 809 Size: 507 3977 1802 Fragments arranged by size: 3977 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2313 3003 Size: 54 1425 50 275 18 177 306 690 Cuts at: 3003 3733 4622 6251 8 Size: 730 889 1629 43 Fragments arranged by size: 1629 1425 889 730 690 306 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3733 1537 Size: 275 1921 4090 Fragments arranged by size: 4090 1921 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2057 3495 4216 1353 Size: 704 1438 721 3423 Fragments arranged by size: 3423 1438 721 704 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2956 3437 4873 5247 5393 387 Size: 1064 138 1367 481 1436 374 146 1280 Fragments arranged by size: 1436 1367 1280 1064 481 374 146 138 EcoICRI GAG'CTC Cuts at: 2964 2964 Size: 6286 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2268 2387 2435 2709 2754 2920 2924 Size: 393 636 119 48 274 45 166 4 Cuts at: 2924 4349 4946 5733 6001 1239 Size: 1425 597 787 268 1524 Fragments arranged by size: 1524 1425 787 636 597 393 274 268 166 119 48 45 4 EcoNI CCTnn'n_nnAGG Cuts at: 3317 3317 Size: 6286 EcoO109I rG'GnC_Cy Cuts at: 2410 3175 3301 3564 3565 2410 Size: 765 126 263 1 5131 Fragments arranged by size: 5131 765 263 126 1 EcoRI G'AATT_C Cuts at: 3595 3973 3595 Size: 378 5908 EcoRV GAT'ATC Cuts at: 2593 2593 Size: 6286 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3361 3361 Size: 6286 FspI TGC'GCA Cuts at: 2879 4792 2879 Size: 1913 4373 HaeII r_GCGC'y Cuts at: 1678 2994 5433 1678 Size: 1316 2439 2531 Fragments arranged by size: 2531 2439 1316 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2554 2586 2690 2722 2839 Size: 32 135 32 982 32 104 32 117 Cuts at: 2839 2871 3256 3288 4927 4959 5001 5033 1373 Size: 32 385 32 1639 32 42 32 2626 Fragments arranged by size: 2626 1639 982 385 135 117 104 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2201 2377 2825 3630 3876 669 Size: 988 281 263 176 448 805 246 3079 Fragments arranged by size: 3079 988 805 448 281 263 246 176 HindIII A'AGCT_T Cuts at: 748 748 Size: 6286 HpaI GTT'AAC Cuts at: 2377 3876 2377 Size: 1499 4787 KpnI G_GTAC'C Cuts at: 3611 3611 Size: 6286 MfeI C'AATT_G Cuts at: 3885 3885 Size: 6286 MluI A'CGCG_T Cuts at: 6162 6162 Size: 6286 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3021 5400 5584 1305 Size: 1716 2379 184 2007 Fragments arranged by size: 2379 2007 1716 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2315 10 Size: 54 1425 343 177 306 3981 Fragments arranged by size: 3981 1425 343 306 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2391 2713 2924 4244 4603 4762 518 Size: 648 1225 322 211 1320 359 159 2042 Fragments arranged by size: 2042 1320 1225 648 359 322 211 159 MspA1I CmG'CkG Cuts at: 1606 3220 3580 3702 4379 5527 5772 1606 Size: 1614 360 122 677 1148 245 2120 Fragments arranged by size: 2120 1614 1148 677 360 245 122 NaeI GCC'GGC Cuts at: 2998 2998 Size: 6286 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4795 Fragments arranged by size: 4795 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6286 NgoMIV G'CCGG_C Cuts at: 2996 2996 Size: 6286 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6286 NotI GC'GGCC_GC Cuts at: 3733 3733 Size: 6286 NsiI A_TGCA'T Cuts at: 2853 2853 Size: 6286 NspI r_CATG'y Cuts at: 1132 1229 3644 5189 1132 Size: 97 2415 1545 2229 Fragments arranged by size: 2415 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6286 PciI A'CATG_T Cuts at: 5185 5185 Size: 6286 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6286 PfoI T'CCnGG_A Cuts at: 2936 2936 Size: 6286 PmeI GTTT'AAAC Cuts at: 3590 3590 Size: 6286 PmlI CAC'GTG Cuts at: 3412 3412 Size: 6286 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4355 4387 5868 5900 4355 Size: 32 1481 32 4741 Fragments arranged by size: 4741 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2410 3175 2410 Size: 765 5521 PshAI GACnn'nnGTC Cuts at: 1613 3509 1613 Size: 1896 4390 PsiI TTA'TAA Cuts at: 3856 3856 Size: 6286 PspOMI G'GGCC_C Cuts at: 3564 3564 Size: 6286 PstI C_TGCA'G Cuts at: 830 3585 3638 830 Size: 2755 53 3478 Fragments arranged by size: 3478 2755 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6286 PvuII CAG'CTG Cuts at: 3220 3220 Size: 6286 SacI G_AGCT'C Cuts at: 2966 2966 Size: 6286 SalI G'TCGA_C Cuts at: 1936 2199 3628 1936 Size: 263 1429 4594 Fragments arranged by size: 4594 1429 263 SapI GCTCTTCn'nnn_ Cuts at: 2057 2057 Size: 6286 SbfI CC_TGCA'GG Cuts at: 3638 3638 Size: 6286 ScaI AGT'ACT Cuts at: 2324 4534 6246 2324 Size: 2210 1712 2364 Fragments arranged by size: 2364 2210 1712 SfcI C'TryA_G Cuts at: 826 1045 2326 3120 3581 3634 4769 5450 5641 Size: 219 1281 794 461 53 1135 681 191 Cuts at: 5641 826 Size: 1471 Fragments arranged by size: 1471 1281 1135 794 681 461 219 191 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6286 SmaI CCC'GGG Cuts at: 1872 2162 3509 3613 1872 Size: 290 1347 104 4545 Fragments arranged by size: 4545 1347 290 104 SmlI C'TyrA_G Cuts at: 820 1017 1943 2560 2804 4390 5291 5553 820 Size: 197 926 617 244 1586 901 262 1553 Fragments arranged by size: 1586 1553 926 901 617 262 244 197 SnaBI TAC'GTA Cuts at: 493 4077 493 Size: 3584 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6286 SphI G_CATG'C Cuts at: 1132 3644 1132 Size: 2512 3774 SspI AAT'ATT Cuts at: 5 52 4210 5 Size: 47 4158 2081 Fragments arranged by size: 4158 2081 47 StuI AGG'CCT Cuts at: 1517 3254 3279 1517 Size: 1737 25 4524 Fragments arranged by size: 4524 1737 25 StyI C'CwwG_G Cuts at: 513 1065 2004 3304 3725 513 Size: 552 939 1300 421 3074 Fragments arranged by size: 3074 1300 939 552 421 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3470 4313 4330 4483 4668 5113 325 Size: 1255 1890 843 17 153 185 445 1498 Fragments arranged by size: 1890 1498 1255 843 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2322 2494 3459 Size: 274 80 33 51 1443 345 172 965 Cuts at: 3459 4532 6244 96 Size: 1073 1712 138 Fragments arranged by size: 1712 1443 1073 965 345 274 172 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2571 2603 2713 2745 3416 3448 3518 3550 6246 Size: 32 110 32 671 32 70 32 2696 Cuts at: 6246 6278 2571 Size: 32 2579 Fragments arranged by size: 2696 2579 671 110 70 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2399 1316 Size: 1083 5203 XbaI T'CTAG_A Cuts at: 3622 3622 Size: 6286 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2712 2712 Size: 6286 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6286 XmaI C'CCGG_G Cuts at: 1870 2160 3507 3611 1870 Size: 290 1347 104 4545 Fragments arranged by size: 4545 1347 290 104 XmnI GAAnn'nnTTC Cuts at: 2256 4415 2256 Size: 2159 4127 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4094 276 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI DraIII FseI FspAI KasI NarI NruI PacI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI