################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BmyI BpmI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1286I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MamI MluI MroI MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tsp45I Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AclI 171 AACGTT AA^CGTT AlwNI 431 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 229 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 345 TGGCCA TGG^CCA BbsI 1306 GAAGAC GAAGAC(2/6) BbvI 329 GCTGC GCAGC(8/12) BclI 561 TGATCA T^GATCA BlpI 299 GCTCAGC GC^T[ACGT]AGC Bpu1102I 299 GCTCAGC GC^T[ACGT]AGC BpuAI 1306 GAAGAC GAAGAC(2/6) BsaAI 208 CACGTA [CT]AC^GT[GA] BsaJI 623 CCTTGG C^C[ACGT][ACGT]GG BsgI 330 CTGCAC GTGCAG(16/14) BspHI 564 TCATGA T^CATGA BspMI 177 ACCTGC ACCTGC(4/8) BsrI 343 ACTGG ACTGG(1/-1) BstBI 1124 TTCGAA TT^CGAA CelII 299 GCTCAGC GC^T[ACGT]AGC DraI 148 TTTAAA TTT^AAA EaeI 345 TGGCCA [CT]^GGCC[GA] EarI 1357 GAAGAG CTCTTC(1/4) EcoT14I 623 CCTTGG C^C[AT][AT]GG FbaI 561 TGATCA T^GATCA Fnu4HI 329 GCTGC GC^[ACGT]GC HincII 25 GTTGAC GT[CT]^[GA]AC HindII 25 GTTGAC GT[CT]^[GA]AC ItaI 329 GCTGC GC^[ACGT]GC Ksp632I 1357 GAAGAG CTCTTC(1/4) MfeI 542 CAATTG C^AATTG MluNI 345 TGGCCA TGG^CCA MscI 345 TGGCCA TGG^CCA MslI 1203 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 542 CAATTG C^AATTG MwoI 703 GCTGTGGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspV 1124 TTCGAA TT^CGAA Psp1406I 171 AACGTT AA^CGTT PstI 179 CTGCAG CTGCA^G RcaI 564 TCATGA T^CATGA SfuI 1124 TTCGAA TT^CGAA StyI 623 CCTTGG C^C[AT][AT]GG TseI 329 GCTGC G^C[AT]GC XbaI 253 TCTAGA T^CTAGA XmnI 229 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AvaII 398 GGACC G^G[AT]CC 697 GGACC BamHI 1032 GGATCC G^GATCC 1134 GGATCC BfaI 254 CTAG C^TAG 874 CTAG BsmAI 1087 GAGAC GTCTC(1/5) 1271 GAGAC BssKI 401 CCTGG ^CC[ACGT]GG 945 CCTGG BstNI 401 CCTGG CC^[AT]GG 945 CCTGG BstYI 1032 GGATCC [GA]^GATC[CT] 1134 GGATCC Cfr13I 398 GGACC G^G[ACGT]CC 697 GGACC EcoRI 280 GAATTC G^AATTC 508 GAATTC EcoRII 401 CCTGG ^CC[AT]GG 945 CCTGG HaeIII 346 GGCC GG^CC 404 GGCC HphI 613 GGTGA GGTGA(8/7) 1202 TCACC MaeI 254 CTAG C^TAG 874 CTAG MaeIII 140 GTTAC ^GT[ACGT]AC 174 GTTAC MflI 1032 GGATCC [GA]^GATC[CT] 1134 GGATCC MspA1I 701 CAGCTG C[AC]G^C[GT]G 1140 CAGCTG MvaI 401 CCTGG CC^[AT]GG 945 CCTGG NlaIV 1032 GGATCC GG[ACGT]^[ACGT]CC 1134 GGATCC PvuII 701 CAGCTG CAG^CTG 1140 CAGCTG Sau96I 398 GGACC G^G[ACGT]CC 697 GGACC ScrFI 401 CCTGG CC^[ACGT]GG 945 CCTGG SmlI 362 CTCAAG C^T[CT][GA]AG 1084 CTTGAG TaqI 1125 TCGA T^CGA 1164 TCGA TfiI 439 GAATC G^A[AT]TC 616 GATTC TspRI 456 CACTG CA[GC]TG[ACGT][ACGT]^ 1096 CACTG TthHB8I 1125 TCGA T^CGA 1164 TCGA XhoII 1032 GGATCC [GA]^GATC[CT] 1134 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI Bsp1407I BsrGI BstF5I DdeI DpnII FokI HinfI MaeII MboI MboII MnlI MseI NdeII NlaIII PleI RsaI Sau3AI SfaNI SfcI SspBI SspI Tru9I Tsp509I