(Circular) (Six-base) MAPSORT of: pFN21AE6406.seq Check: 2053 from: 1 to: 6079 pFN21AE6406 6079 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3889 278 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3422 1937 Size: 1485 4594 Acc65I G'GTAC_C Cuts at: 3400 3400 Size: 6079 AclI AA'CG_TT Cuts at: 1563 2177 4206 4579 1563 Size: 614 2029 373 3063 Fragments arranged by size: 3063 2029 614 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2558 3219 4142 5526 5794 1632 Size: 926 661 923 1384 268 1917 Fragments arranged by size: 1917 1384 926 923 661 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5882 AflIII A'CryG_T Cuts at: 1203 4978 5955 1203 Size: 3775 977 1327 Fragments arranged by size: 3775 1327 977 AgeI A'CCGG_T Cuts at: 4910 4910 Size: 6079 AhdI GACnn_n'nnGTC Cuts at: 4808 4808 Size: 6079 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6079 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6079 AlwNI CAG_nnn'CTG Cuts at: 2441 3822 5394 2441 Size: 1381 1572 3126 Fragments arranged by size: 3126 1572 1381 ApaLI G'TGCA_C Cuts at: 4136 5292 4136 Size: 1156 4923 ApoI r'AATT_y Cuts at: 1493 2048 2116 2237 2285 2431 2471 2513 2927 Size: 555 68 121 48 146 40 42 414 Cuts at: 2927 3221 3388 3614 3766 5875 1493 Size: 294 167 226 152 2109 1697 Fragments arranged by size: 2109 1697 555 414 294 226 167 152 146 121 68 48 42 40 AseI AT'TA_AT Cuts at: 160 1032 4633 160 Size: 872 3601 1606 Fragments arranged by size: 3601 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 AvaI C'yCGr_G Cuts at: 1870 1943 3404 1870 Size: 73 1461 4545 Fragments arranged by size: 4545 1461 73 BamHI G'GATC_C Cuts at: 3037 3139 3409 3776 3037 Size: 102 270 367 5340 Fragments arranged by size: 5340 367 270 102 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3400 4855 618 Size: 325 217 627 1613 1455 1842 Fragments arranged by size: 1842 1613 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3398 1391 Size: 2007 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3318 928 Size: 971 1419 3689 Fragments arranged by size: 3689 1419 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4270 4304 1903 Size: 34 2333 34 3678 Fragments arranged by size: 3678 2333 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3971 5181 3971 Size: 1210 4869 BclI T'GATC_A Cuts at: 1548 2566 1548 Size: 1018 5061 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4690 136 Size: 107 122 71 631 605 337 2681 1525 Fragments arranged by size: 2681 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6074 6074 Size: 6079 BlpI GC'TnA_GC Cuts at: 2305 3496 2305 Size: 1191 4888 Bme1580I G_kGCm'C Cuts at: 1790 4140 5296 1790 Size: 2350 1156 2573 Fragments arranged by size: 2573 2350 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6079 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3810 4768 442 Size: 416 53 83 399 24 2393 958 1753 Fragments arranged by size: 2393 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6079 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4739 1239 Size: 3500 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2352 3110 4204 5069 5367 2352 Size: 758 1094 865 298 3064 Fragments arranged by size: 3064 1094 865 758 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4742 882 Size: 617 3243 2219 Fragments arranged by size: 3243 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2215 3870 493 Size: 711 1011 1655 2702 Fragments arranged by size: 2702 1655 1011 711 BsaBI GATnn'nnATC Cuts at: 6073 6073 Size: 6079 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3886 4268 4903 275 Size: 53 83 186 936 2353 382 635 1451 Fragments arranged by size: 2353 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4512 4910 5184 5331 5742 4512 Size: 398 274 147 411 4849 Fragments arranged by size: 4849 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5854 Fragments arranged by size: 5854 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6079 BseYI C'CCAG_C Cuts at: 3143 5282 5778 3143 Size: 2139 496 3444 Fragments arranged by size: 3444 2139 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2320 1163 Size: 573 584 4922 Fragments arranged by size: 4922 584 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3529 4290 5318 1540 Size: 275 186 1528 761 1028 2301 Fragments arranged by size: 2301 1528 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4140 4225 5296 4140 Size: 85 1071 4923 Fragments arranged by size: 4923 1071 85 BsmI GAATG_Cn' Cuts at: 3590 3683 3590 Size: 93 5986 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3398 4140 4225 5296 1391 Size: 399 1608 742 85 1071 2174 Fragments arranged by size: 2174 1608 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5742 5742 Size: 6079 BspHI T'CATG_A Cuts at: 2569 3968 5698 2569 Size: 1399 1730 2950 Fragments arranged by size: 2950 1730 1399 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2191 3434 844 Size: 1347 1243 3489 Fragments arranged by size: 3489 1347 1243 BsrBI CCG'CTC Cuts at: 1394 1823 3966 1394 Size: 429 2143 3507 Fragments arranged by size: 3507 2143 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4574 4748 65 Size: 1183 3326 174 1396 Fragments arranged by size: 3326 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4723 4910 1700 Size: 110 2913 187 2869 Fragments arranged by size: 2913 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2198 2795 3149 96 Size: 2102 597 354 3026 Fragments arranged by size: 3026 2102 597 354 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6079 BssSI C'ACGA_G Cuts at: 4139 5151 4139 Size: 1012 5067 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6079 BstBI TT'CG_AA Cuts at: 3130 4883 3130 Size: 1753 4326 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6079 BstYI r'GATC_y Cuts at: 1972 3037 3139 3409 3776 4161 4178 5630 5716 Size: 1065 102 270 367 385 17 1452 86 Cuts at: 5716 5728 6074 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1065 385 367 346 270 102 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4588 Fragments arranged by size: 4588 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5340 Fragments arranged by size: 5340 683 56 BtsI GCAGTG_nn' Cuts at: 3100 3595 4389 4409 3100 Size: 495 794 20 4770 Fragments arranged by size: 4770 794 495 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6079 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6044 DraI TTT'AAA Cuts at: 2155 3383 2155 Size: 1228 4851 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5086 809 Size: 507 3770 1802 Fragments arranged by size: 3770 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2350 3526 4415 Size: 54 1425 50 275 18 520 1176 889 Cuts at: 4415 6044 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1176 889 520 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3526 1537 Size: 275 1714 4090 Fragments arranged by size: 4090 1714 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3357 4009 1353 Size: 2004 652 3423 Fragments arranged by size: 3423 2004 652 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4666 5040 5186 387 Size: 1064 138 3077 374 146 1280 Fragments arranged by size: 3077 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2558 3219 4142 4739 5526 5794 1239 Size: 393 926 661 923 597 787 268 1524 Fragments arranged by size: 1524 926 923 787 661 597 393 268 EcoRI G'AATT_C Cuts at: 2285 2513 3388 3766 2285 Size: 228 875 378 4598 Fragments arranged by size: 4598 875 378 228 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2513 3332 2513 Size: 819 5260 FspI TGC'GCA Cuts at: 4585 4585 Size: 6079 HaeII r_GCGC'y Cuts at: 1678 5226 1678 Size: 3548 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2429 2461 3275 3307 4720 Size: 32 135 32 857 32 814 32 1413 Cuts at: 4720 4752 4794 4826 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1413 857 814 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2032 3423 3669 669 Size: 988 281 94 1391 246 3079 Fragments arranged by size: 3079 1391 988 281 246 94 HindIII A'AGCT_T Cuts at: 748 748 Size: 6079 HpaI GTT'AAC Cuts at: 3669 3669 Size: 6079 KpnI G_GTAC'C Cuts at: 3404 3404 Size: 6079 MfeI C'AATT_G Cuts at: 2547 3678 2547 Size: 1131 4948 MluI A'CGCG_T Cuts at: 5955 5955 Size: 6079 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5193 5377 1305 Size: 3888 184 2007 Fragments arranged by size: 3888 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2352 10 Size: 54 1425 343 520 3737 Fragments arranged by size: 3737 1425 520 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3212 4037 4396 4555 518 Size: 648 2046 825 359 159 2042 Fragments arranged by size: 2046 2042 825 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2708 3147 3495 4172 5320 5565 1606 Size: 1102 439 348 677 1148 245 2120 Fragments arranged by size: 2120 1148 1102 677 439 348 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4588 Fragments arranged by size: 4588 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6079 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6079 NotI GC'GGCC_GC Cuts at: 3526 3526 Size: 6079 NspI r_CATG'y Cuts at: 1132 1229 3437 4982 1132 Size: 97 2208 1545 2229 Fragments arranged by size: 2229 2208 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6079 PciI A'CATG_T Cuts at: 4978 4978 Size: 6079 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6079 PmeI GTTT'AAAC Cuts at: 3383 3383 Size: 6079 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4148 4180 5661 5693 4148 Size: 32 1481 32 4534 Fragments arranged by size: 4534 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6079 PsiI TTA'TAA Cuts at: 3649 3649 Size: 6079 PstI C_TGCA'G Cuts at: 830 2188 3431 830 Size: 1358 1243 3478 Fragments arranged by size: 3478 1358 1243 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6079 PvuII CAG'CTG Cuts at: 2708 3147 2708 Size: 439 5640 SalI G'TCGA_C Cuts at: 1936 3421 1936 Size: 1485 4594 SbfI CC_TGCA'GG Cuts at: 3431 3431 Size: 6079 ScaI AGT'ACT Cuts at: 4327 6039 4327 Size: 1712 4367 SfcI C'TryA_G Cuts at: 826 1045 2013 2184 2275 3427 4562 5243 5434 Size: 219 968 171 91 1152 1135 681 191 Cuts at: 5434 826 Size: 1471 Fragments arranged by size: 1471 1152 1135 968 681 219 191 171 91 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6079 SmaI CCC'GGG Cuts at: 1872 3406 1872 Size: 1534 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2367 3089 4183 5084 5346 820 Size: 197 926 424 722 1094 901 262 1553 Fragments arranged by size: 1553 1094 926 901 722 424 262 197 SnaBI TAC'GTA Cuts at: 493 3870 493 Size: 3377 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6079 SphI G_CATG'C Cuts at: 1132 3437 1132 Size: 2305 3774 SspI AAT'ATT Cuts at: 5 52 2425 2558 2957 4003 5 Size: 47 2373 133 399 1046 2081 Fragments arranged by size: 2373 2081 1046 399 133 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6079 StyI C'CwwG_G Cuts at: 513 1065 2004 2628 3518 513 Size: 552 939 624 890 3074 Fragments arranged by size: 3074 939 890 624 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2074 2316 4106 4123 4276 4461 4906 Size: 1255 494 242 1790 17 153 185 445 Cuts at: 4906 325 Size: 1498 Fragments arranged by size: 1790 1498 1255 494 445 242 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2198 2795 3149 Size: 274 80 33 51 1443 221 597 354 Cuts at: 3149 4325 6037 96 Size: 1176 1712 138 Fragments arranged by size: 1712 1443 1176 597 354 274 221 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2694 2726 6039 6071 2694 Size: 32 3313 32 2702 Fragments arranged by size: 3313 2702 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6079 XbaI T'CTAG_A Cuts at: 2258 3415 2258 Size: 1157 4922 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6079 XmaI C'CCGG_G Cuts at: 1870 3404 1870 Size: 1534 4545 XmnI GAAnn'nnTTC Cuts at: 2238 4208 2238 Size: 1970 4109 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3887 276 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI