################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII Acc65I AccI AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BclI BlnI BsaBI BsaI BsiWI BsmBI BsmFI Bsp1407I BspDI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstXI BstZ17I Cfr10I ClaI CpoI DraIII DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HpaI KpnI KspI MamI MfeI MluI MslI MunI NaeI NdeI NgoMIV NheI NotI NsiI PacI PflFI PinAI PmeI PshAI PshBI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccIII 1940 TCCGGA T^CCGGA AclI 1364 AACGTT AA^CGTT AcyI 2031 GGCGCC G[GA]^CG[CT]C AflII 675 CTTAAG C^TTAAG AocI 1653 CCTGAGG CC^T[ACGT]AGG ApaI 1719 GGGCCC GGGCC^C BalI 1439 TGGCCA TGG^CCA BamHI 71 GGATCC G^GATCC BanI 2031 GGCGCC G^G[CT][GA]CC BbeI 2031 GGCGCC GGCGC^C BbvCI 1707 GCTGAGG CCTCAGC(-5/-2) BcgI 1769 CGAGGGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 1998 CCGGG CC^[GC]GG BfrI 675 CTTAAG C^TTAAG BglI 352 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 162 AGATCT A^GATCT BlpI 979 GCTGAGC GC^T[ACGT]AGC Bpu1102I 979 GCTGAGC GC^T[ACGT]AGC BsaHI 2031 GGCGCC G[GA]^CG[CT]C BsaWI 1940 TCCGGA [AT]^CCGG[AT] BseAI 1940 TCCGGA T^CCGGA BsiEI 2266 CGGCCG CG[GA][CT]^CG BspEI 1940 TCCGGA T^CCGGA BspLU11I 2097 ACATGT A^CATGT BspMI 1703 ACCTGC ACCTGC(4/8) BsrDI 1724 CATTGC GCAATG(2/0) BstBI 1990 TTCGAA TT^CGAA Bsu36I 1653 CCTGAGG CC^T[ACGT]AGG CelII 979 GCTGAGC GC^T[ACGT]AGC EagI 2266 CGGCCG C^GGCCG EclXI 2266 CGGCCG C^GGCCG Eco52I 2266 CGGCCG C^GGCCG Eco81I 1653 CCTGAGG CC^T[ACGT]AGG EcoNI 1455 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Hin1I 2031 GGCGCC G[GA]^CG[CT]C HindIII 268 AAGCTT A^AGCTT KasI 2031 GGCGCC G^GCGCC MluNI 1439 TGGCCA TGG^CCA MroI 1940 TCCGGA T^CCGGA MscI 1439 TGGCCA TGG^CCA NarI 2031 GGCGCC GG^CGCC NciI 1998 CCGGG CC^[GC]GG NruI 2124 TCGCGA TCG^CGA NspV 1990 TTCGAA TT^CGAA PaeR7I 1767 CTCGAG C^TCGAG PflMI 1251 CCAGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 1364 AACGTT AA^CGTT SfoI 2031 GGCGCC GGC^GCC SfuI 1990 TTCGAA TT^CGAA SspI 439 AATATT AAT^ATT StuI 1091 AGGCCT AGG^CCT Van91I 1251 CCAGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1043 CCATTAACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1767 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 632 GTAC GT^AC 2088 GTAC AflIII 945 ACACGT A^C[GA][CT]GT 2097 ACATGT AlwNI 175 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 1534 CAGAATCTG Asp700I 483 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1555 GAAGTGCTTC AvaI 1061 CTCGGG C^[CT]CG[GA]G 1767 CTCGAG BbrPI 946 CACGTG CAC^GTG 1759 CACGTG BbsI 868 GAAGAC GAAGAC(2/6) 1944 GAAGAC BciVI 996 GGATAC GTATCC(6/5) 2288 GGATAC BpuAI 868 GAAGAC GAAGAC(2/6) 1944 GAAGAC BsaAI 946 CACGTG [CT]AC^GT[GA] 1759 CACGTG BsgI 758 GTGCAG GTGCAG(16/14) 1189 GTGCAG BsmI 1271 GAATGC GAATGC(1/-1) 1345 GAATGC BsoBI 1061 CTCGGG C^[CT]CG[GA]G 1767 CTCGAG BstAPI 1080 GCAGACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1923 GCAGCGGATGC DraI 564 TTTAAA TTT^AAA 2106 TTTAAA EaeI 1439 TGGCCA [CT]^GGCC[GA] 2266 CGGCCG HaeII 1239 GGCGCT [GA]GCGC^[CT] 2031 GGCGCC PmaCI 946 CACGTG CAC^GTG 1759 CACGTG PmlI 946 CACGTG CAC^GTG 1759 CACGTG RsaI 632 GTAC GT^AC 2088 GTAC SapI 721 GAAGAGC GCTCTTC(1/4) 1986 GCTCTTC TaqI 1768 TCGA T^CGA 1991 TCGA TthHB8I 1768 TCGA T^CGA 1991 TCGA XmnI 483 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1555 GAAGTGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AccII AciI AcsI AluI AlwI ApoI AspHI AvaII BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII