(Linear) (Six-base) MAPSORT of: KIEE1500.seq Check: 4898 from: 1 to: 2301 KIEE1500.orf With 182 enzymes: SgfI * August 26, 2010 17:36 .. AclI AA'CG_TT Cuts at: 0 1365 2301 Size: 1365 936 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 1423 2301 Size: 34 1389 878 Fragments arranged by size: 1389 878 34 AflII C'TTAA_G Cuts at: 0 675 2301 Size: 675 1626 AflIII A'CryG_T Cuts at: 0 945 2097 2301 Size: 945 1152 204 Fragments arranged by size: 1152 945 204 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1204 1220 1346 2301 Size: 1204 16 126 955 Fragments arranged by size: 1204 955 126 16 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 785 817 1774 1806 2301 Size: 785 32 957 32 495 Fragments arranged by size: 957 785 495 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 180 1539 2301 Size: 180 1359 762 Fragments arranged by size: 1359 762 180 ApaI G_GGCC'C Cuts at: 0 1723 2301 Size: 1723 578 ApoI r'AATT_y Cuts at: 0 313 987 2110 2301 Size: 313 674 1123 191 Fragments arranged by size: 1123 674 313 191 AvaI C'yCGr_G Cuts at: 0 1061 1767 2301 Size: 1061 706 534 Fragments arranged by size: 1061 706 534 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 190 223 2281 2301 Size: 190 33 2058 20 Fragments arranged by size: 2058 190 33 20 BamHI G'GATC_C Cuts at: 0 71 2301 Size: 71 2230 BanI G'GyrC_C Cuts at: 0 2031 2301 Size: 2031 270 BanII G_rGCy'C Cuts at: 0 803 1160 1723 1940 1988 2301 Size: 803 357 563 217 48 313 Fragments arranged by size: 803 563 357 313 217 48 BbeI G_GCGC'C Cuts at: 0 2035 2301 Size: 2035 266 BbsI GAAGACnn'nnnn_ Cuts at: 0 875 1951 2301 Size: 875 1076 350 Fragments arranged by size: 1076 875 350 BbvCI CC'TCA_GC Cuts at: 0 1708 2301 Size: 1708 593 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1758 1792 2301 Size: 1758 34 509 Fragments arranged by size: 1758 509 34 BciVI GTATCCnnnnn_n' Cuts at: 0 990 2282 2301 Size: 990 1292 19 Fragments arranged by size: 1292 990 19 BglI GCCn_nnn'nGGC Cuts at: 0 358 2301 Size: 358 1943 BglII A'GATC_T Cuts at: 0 162 2301 Size: 162 2139 BlpI GC'TnA_GC Cuts at: 0 980 2301 Size: 980 1321 Bme1580I G_kGCm'C Cuts at: 0 1723 2301 Size: 1723 578 BmrI ACTGGGnnnn_n' Cuts at: 0 447 2301 Size: 447 1854 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 138 1075 1096 1231 1285 1339 1477 1549 Size: 138 937 21 135 54 54 138 72 Cuts at: 1549 1570 1612 1666 1912 1954 2233 2301 Size: 21 42 54 246 42 279 68 Fragments arranged by size: 937 279 246 138 138 135 72 68 54 54 54 42 42 21 21 Bpu10I CC'TnA_GC Cuts at: 0 1037 1708 2301 Size: 1037 671 593 Fragments arranged by size: 1037 671 593 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 842 1433 1648 2185 2301 Size: 842 591 215 537 116 Fragments arranged by size: 842 591 537 215 116 BsaAI yAC'GTr Cuts at: 0 948 1761 2301 Size: 948 813 540 Fragments arranged by size: 948 813 540 BsaHI Gr'CG_yC Cuts at: 0 2032 2301 Size: 2032 269 BsaWI w'CCGG_w Cuts at: 0 1940 2301 Size: 1940 361 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 785 815 1204 1234 2301 Size: 785 30 389 30 1067 Fragments arranged by size: 1067 785 389 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 655 1114 1474 1687 2200 2203 2301 Size: 655 459 360 213 513 3 98 Fragments arranged by size: 655 513 459 360 213 98 3 BseYI C'CCAG_C Cuts at: 0 173 2301 Size: 173 2128 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 779 1210 2301 Size: 779 431 1091 Fragments arranged by size: 1091 779 431 BsiEI CG_ry'CG Cuts at: 0 2269 2301 Size: 2269 32 BsiHKAI G_wGCw'C Cuts at: 0 803 1160 1940 1988 2301 Size: 803 357 780 48 313 Fragments arranged by size: 803 780 357 313 48 BsmI GAATG_Cn' Cuts at: 0 1277 1351 2301 Size: 1277 74 950 Fragments arranged by size: 1277 950 74 Bsp1286I G_dGCh'C Cuts at: 0 803 1160 1723 1940 1988 2301 Size: 803 357 563 217 48 313 Fragments arranged by size: 803 563 357 313 217 48 BspEI T'CCGG_A Cuts at: 0 1940 2301 Size: 1940 361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1712 2301 Size: 1712 589 BsrDI GCAATG_nn' Cuts at: 0 1723 2301 Size: 1723 578 BstAPI GCAn_nnn'nTGC Cuts at: 0 1086 1929 2301 Size: 1086 843 372 Fragments arranged by size: 1086 843 372 BstBI TT'CG_AA Cuts at: 0 1991 2301 Size: 1991 310 BstYI r'GATC_y Cuts at: 0 71 162 1842 2301 Size: 71 91 1680 459 Fragments arranged by size: 1680 459 91 71 Bsu36I CC'TnA_GG Cuts at: 0 1654 2301 Size: 1654 647 BtgI C'CryG_G Cuts at: 0 76 1442 1838 2293 2301 Size: 76 1366 396 455 8 Fragments arranged by size: 1366 455 396 76 8 BtsI GCAGTG_nn' Cuts at: 0 845 1688 2301 Size: 845 843 613 Fragments arranged by size: 845 843 613 DraI TTT'AAA Cuts at: 0 566 2108 2301 Size: 566 1542 193 Fragments arranged by size: 1542 566 193 EaeI y'GGCC_r Cuts at: 0 1439 2266 2301 Size: 1439 827 35 Fragments arranged by size: 1439 827 35 EagI C'GGCC_G Cuts at: 0 2266 2301 Size: 2266 35 EarI CTCTTCn'nnn_ Cuts at: 0 26 716 907 1475 1993 2301 Size: 26 690 191 568 518 308 Fragments arranged by size: 690 568 518 308 191 26 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1684 2301 Size: 1684 617 EcoICRI GAG'CTC Cuts at: 0 801 1158 1938 1986 2301 Size: 801 357 780 48 315 Fragments arranged by size: 801 780 357 315 48 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 138 1075 1096 1231 1285 1339 1423 Size: 34 104 937 21 135 54 54 84 Cuts at: 1423 1477 1549 1570 1612 1666 1912 1954 2233 Size: 54 72 21 42 54 246 42 279 Cuts at: 2233 2301 Size: 68 Fragments arranged by size: 937 279 246 135 104 84 72 68 54 54 54 54 42 42 34 21 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 1459 2301 Size: 1459 842 EcoO109I rG'GnC_Cy Cuts at: 0 1200 1650 1658 1701 1719 1782 2301 Size: 1200 450 8 43 18 63 519 Fragments arranged by size: 1200 519 450 63 43 18 8 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 286 2301 Size: 286 2015 HaeII r_GCGC'y Cuts at: 0 1243 2035 2301 Size: 1243 792 266 Fragments arranged by size: 1243 792 266 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 154 186 468 500 995 1027 2301 Size: 154 32 282 32 495 32 1274 Fragments arranged by size: 1274 495 282 154 32 32 32 HindIII A'AGCT_T Cuts at: 0 268 2301 Size: 268 2033 KasI G'GCGC_C Cuts at: 0 2031 2301 Size: 2031 270 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1134 1825 2301 Size: 1134 691 476 Fragments arranged by size: 1134 691 476 MscI TGG'CCA Cuts at: 0 1441 2301 Size: 1441 860 MspA1I CmG'CkG Cuts at: 0 1282 1590 1632 1926 2301 Size: 1282 308 42 294 375 Fragments arranged by size: 1282 375 308 294 42 NarI GG'CG_CC Cuts at: 0 2032 2301 Size: 2032 269 NcoI C'CATG_G Cuts at: 0 1442 1838 2293 2301 Size: 1442 396 455 8 Fragments arranged by size: 1442 455 396 8 NruI TCG'CGA Cuts at: 0 2126 2301 Size: 2126 175 NspI r_CATG'y Cuts at: 0 561 2101 2199 2301 Size: 561 1540 98 102 Fragments arranged by size: 1540 561 102 98 PciI A'CATG_T Cuts at: 0 2097 2301 Size: 2097 204 PflMI CCAn_nnn'nTGG Cuts at: 0 1257 2301 Size: 1257 1044 PfoI T'CCnGG_A Cuts at: 0 1997 2301 Size: 1997 304 PmlI CAC'GTG Cuts at: 0 948 1761 2301 Size: 948 813 540 Fragments arranged by size: 948 813 540 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 785 817 2301 Size: 785 32 1484 Fragments arranged by size: 1484 785 32 PpuMI rG'GwC_Cy Cuts at: 0 1658 1701 1782 2301 Size: 1658 43 81 519 Fragments arranged by size: 1658 519 81 43 PsiI TTA'TAA Cuts at: 0 2149 2301 Size: 2149 152 PspOMI G'GGCC_C Cuts at: 0 1719 2301 Size: 1719 582 PspXI vC'TCGA_Gb Cuts at: 0 1767 2301 Size: 1767 534 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1355 1387 2301 Size: 1355 32 914 Fragments arranged by size: 1355 914 32 PstI C_TGCA'G Cuts at: 0 1622 1637 1683 1809 1955 2301 Size: 1622 15 46 126 146 346 Fragments arranged by size: 1622 346 146 126 46 15 PvuII CAG'CTG Cuts at: 0 1282 1590 1632 2301 Size: 1282 308 42 669 Fragments arranged by size: 1282 669 308 42 SacI G_AGCT'C Cuts at: 0 803 1160 1940 1988 2301 Size: 803 357 780 48 313 Fragments arranged by size: 803 780 357 313 48 SapI GCTCTTCn'nnn_ Cuts at: 0 716 1993 2301 Size: 716 1277 308 Fragments arranged by size: 1277 716 308 SfcI C'TryA_G Cuts at: 0 1618 1633 1679 1805 1855 1951 2301 Size: 1618 15 46 126 50 96 350 Fragments arranged by size: 1618 350 126 96 50 46 15 SfoI GGC'GCC Cuts at: 0 2033 2301 Size: 2033 268 SmlI C'TyrA_G Cuts at: 0 675 857 1448 1663 1767 2200 2301 Size: 675 182 591 215 104 433 101 Fragments arranged by size: 675 591 433 215 182 104 101 SspI AAT'ATT Cuts at: 0 441 2301 Size: 441 1860 StuI AGG'CCT Cuts at: 0 1093 2301 Size: 1093 1208 StyI C'CwwG_G Cuts at: 0 905 1442 1838 2252 2293 2301 Size: 905 537 396 414 41 8 Fragments arranged by size: 905 537 414 396 41 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1249 2301 Size: 1249 1052 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1050 2301 Size: 1050 1251 XhoI C'TCGA_G Cuts at: 0 1767 2301 Size: 1767 534 XmnI GAAnn'nnTTC Cuts at: 0 487 1559 2301 Size: 487 1072 742 Fragments arranged by size: 1072 742 487 Enzymes that do cut: AclI AcuI AflII AflIII AlfI AloI AlwNI ApaI ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI BstAPI BstBI BstYI Bsu36I BtgI BtsI DraI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII KasI MmeI MscI MspA1I NarI NcoI NruI NspI PciI PflMI PfoI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuII SacI SapI SfcI SfoI SmlI SspI StuI StyI TaqII XcmI XhoI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AgeI AhdI AleI ApaLI AscI AseI AsiSI AvrII BclI BfrBI BmgBI BmtI BplI BsaI BsaBI BsiWI BsmBI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraIII DrdI EcoRI EcoRV FseI FspI FspAI HincII HpaI KpnI MfeI MluI MslI NaeI NdeI NgoMIV NheI NotI NsiI PacI PasI PmeI PshAI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TatI TstI Tth111I XbaI XmaI ZraI