Restriction map for
pFN21AE3633
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BalI
BbeI BbvCI BciVI BclI BfrI
BglII BlnI BsaI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DrdI Eam1105I EcoRI EcoT22I
FbaI FseI HindIII HpaI KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MseI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Aor51HI 845 AGCGCT AGC^GCT
AspI 1435 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1016 TGCGCA TGC^GCA
BamHI 366 GGATCC G^GATCC
BbsI 120 GTCTTC GAAGAC(2/6)
BcgI 294 CGAGAACTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 385 CTAG C^TAG
BlpI 726 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 726 GCTCAGC GC^T[ACGT]AGC
BpuAI 120 GTCTTC GAAGAC(2/6)
BsaBI 1456 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 276 GAATGC GAATGC(1/-1)
BssSI 1123 CTCGTG CACGAG(-5/-1)
CelII 726 GCTCAGC GC^T[ACGT]AGC
DraIII 796 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2432 CGGCCG C^GGCCG
EclXI 2432 CGGCCG C^GGCCG
Eco47III 845 AGCGCT AGC^GCT
Eco52I 2432 CGGCCG C^GGCCG
EcoNI 792 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1840 GATATC GAT^ATC
FspI 1016 TGCGCA TGC^GCA
HincII 1171 GTCAAC GT[CT]^[GA]AC
HindII 1171 GTCAAC GT[CT]^[GA]AC
MaeI 385 CTAG C^TAG
MamI 1456 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflFI 1435 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 702 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 1085 AGTACT AGT^ACT
SexAI 797 ACCAGGT A^CC[AT]GGT
SfiI 2220 GGCCTCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 806 CCCGGG CCC^GGG
Tth111I 1435 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 702 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 806 CCCGGG C^CCGGG
XmaI 806 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 1168 GGCGTC G[GA]^CG[CT]C
2437 GGCGTC
AflIII 1175 ACACGT A^C[GA][CT]GT
1177 ACGTGT
AocI 1281 CCTGAGG CC^T[ACGT]AGG
2161 CCTCAGG
BanI 93 GGTGCC G^G[CT][GA]CC
2131 GGCACC
BbrPI 979 CACGTG CAC^GTG
1176 CACGTG
BsaAI 979 CACGTG [CT]AC^GT[GA]
1176 CACGTG
BsaHI 1168 GGCGTC G[GA]^CG[CT]C
2437 GGCGTC
BstXI 1429 CCAGAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1934 CCATGGACGTGG
BstYI 366 GGATCC [GA]^GATC[CT]
1613 AGATCC
Bsu36I 1281 CCTGAGG CC^T[ACGT]AGG
2161 CCTCAGG
Eco81I 1281 CCTGAGG CC^T[ACGT]AGG
2161 CCTCAGG
EcoT14I 1238 CCATGG C^C[AT][AT]GG
1934 CCATGG
Hin1I 1168 GGCGTC G[GA]^CG[CT]C
2437 GGCGTC
MflI 366 GGATCC [GA]^GATC[CT]
1613 AGATCC
NcoI 1238 CCATGG C^CATGG
1934 CCATGG
NspI 2324 ACATGC [GA]CATG^[CT]
2333 GCATGT
PmaCI 979 CACGTG CAC^GTG
1176 CACGTG
PmlI 979 CACGTG CAC^GTG
1176 CACGTG
PvuII 1117 CAGCTG CAG^CTG
2022 CAGCTG
SapI 1029 GCTCTTC GCTCTTC(1/4)
2256 GCTCTTC
SbfI 912 CCTGCAGG CCTGCA^GG
2373 CCTGCAGG
Sse8387I 912 CCTGCAGG CCTGCA^GG
2373 CCTGCAGG
StyI 1238 CCATGG C^C[AT][AT]GG
1934 CCATGG
XhoII 366 GGATCC [GA]^GATC[CT]
1613 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanII BbvI BcnI BglI BmyI
BpmI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoO65I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI PpuMI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TseI
Tsp45I TspRI TthHB8I XcmI