(Circular) (Six-base) MAPSORT of: pFN21AE4332.seq Check: 436 from: 1 to: 5728 pFN21AE4332 5728 bp With 182 enzymes: SgfI * March 28, 2012 13:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2742 2742 Size: 5728 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3538 278 Size: 53 83 186 936 2002 2468 Fragments arranged by size: 2468 2002 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3071 1937 Size: 1134 4594 Acc65I G'GTAC_C Cuts at: 3049 3049 Size: 5728 AclI AA'CG_TT Cuts at: 1563 3855 4228 1563 Size: 2292 373 3063 Fragments arranged by size: 3063 2292 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3791 5175 5443 1632 Size: 2159 1384 268 1917 Fragments arranged by size: 2159 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5531 AflIII A'CryG_T Cuts at: 1203 2472 4627 5604 1203 Size: 1269 2155 977 1327 Fragments arranged by size: 2155 1327 1269 977 AgeI A'CCGG_T Cuts at: 2717 4559 2717 Size: 1842 3886 AhdI GACnn_n'nnGTC Cuts at: 4457 4457 Size: 5728 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5728 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2301 1184 Size: 1117 4611 AlwNI CAG_nnn'CTG Cuts at: 3471 5043 3471 Size: 1572 4156 ApaI G_GGCC'C Cuts at: 2414 2529 2570 2414 Size: 115 41 5572 Fragments arranged by size: 5572 115 41 ApaLI G'TGCA_C Cuts at: 3785 4941 3785 Size: 1156 4572 ApoI r'AATT_y Cuts at: 1493 3037 3263 3415 5524 1493 Size: 1544 226 152 2109 1697 Fragments arranged by size: 2109 1697 1544 226 152 AseI AT'TA_AT Cuts at: 160 1032 4282 160 Size: 872 3250 1606 Fragments arranged by size: 3250 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5728 AvaI C'yCGr_G Cuts at: 1870 1943 2638 3053 1870 Size: 73 695 415 4545 Fragments arranged by size: 4545 695 415 73 BamHI G'GATC_C Cuts at: 3058 3425 3058 Size: 367 5361 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3049 4504 618 Size: 325 217 627 1262 1455 1842 Fragments arranged by size: 1842 1455 1262 627 325 217 BanII G_rGCy'C Cuts at: 1391 2414 2465 2529 2570 3027 3047 1391 Size: 1023 51 64 41 457 20 4072 Fragments arranged by size: 4072 1023 457 64 51 41 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4757 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3919 3953 1903 Size: 34 1982 34 3678 Fragments arranged by size: 3678 1982 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3620 4830 3620 Size: 1210 4518 BclI T'GATC_A Cuts at: 1548 1548 Size: 5728 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4339 136 Size: 107 122 71 631 605 2667 1525 Fragments arranged by size: 2667 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5723 5723 Size: 5728 BlpI GC'TnA_GC Cuts at: 3021 3145 3021 Size: 124 5604 Bme1580I G_kGCm'C Cuts at: 1790 2414 2529 2570 3789 4945 1790 Size: 624 115 41 1219 1156 2573 Fragments arranged by size: 2573 1219 1156 624 115 41 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5728 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2340 3459 4417 Size: 416 53 83 399 24 923 1119 958 Cuts at: 4417 442 Size: 1753 Fragments arranged by size: 1753 1119 958 923 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5728 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4388 1239 Size: 3149 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3853 4718 5016 3853 Size: 865 298 4565 Fragments arranged by size: 4565 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2248 4391 882 Size: 617 749 2143 2219 Fragments arranged by size: 2219 2143 749 617 BsaAI yAC'GTr Cuts at: 493 1204 3519 493 Size: 711 2315 2702 Fragments arranged by size: 2702 2315 711 BsaBI GATnn'nnATC Cuts at: 5722 5722 Size: 5728 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3535 3917 4552 275 Size: 53 83 186 936 2002 382 635 1451 Fragments arranged by size: 2002 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2717 4161 4559 4833 4980 5391 2717 Size: 1444 398 274 147 411 3054 Fragments arranged by size: 3054 1444 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2079 2109 2421 2451 1178 Size: 30 165 30 676 30 312 30 4455 Fragments arranged by size: 4455 676 312 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2771 2774 1186 Size: 1585 3 4140 Fragments arranged by size: 4140 1585 3 BseYI C'CCAG_C Cuts at: 2403 4931 5427 2403 Size: 2528 496 2704 Fragments arranged by size: 2704 2528 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2168 1163 Size: 573 432 4723 Fragments arranged by size: 4723 573 432 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3178 3939 4967 1540 Size: 275 186 1177 761 1028 2301 Fragments arranged by size: 2301 1177 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2232 2465 3789 3874 4945 2232 Size: 233 1324 85 1071 3015 Fragments arranged by size: 3015 1324 1071 233 85 BsmI GAATG_Cn' Cuts at: 3239 3332 3239 Size: 93 5635 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2232 2414 2465 2529 2570 3027 3047 Size: 399 442 182 51 64 41 457 20 Cuts at: 3047 3789 3874 4945 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 457 442 399 182 85 64 51 41 20 BspEI T'CCGG_A Cuts at: 5391 5391 Size: 5728 BspHI T'CATG_A Cuts at: 3617 5347 3617 Size: 1730 3998 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2742 3083 844 Size: 1898 341 3489 Fragments arranged by size: 3489 1898 341 BsrBI CCG'CTC Cuts at: 1394 1823 3615 1394 Size: 429 1792 3507 Fragments arranged by size: 3507 1792 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2153 4223 4397 65 Size: 1183 905 2070 174 1396 Fragments arranged by size: 2070 1396 1183 905 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2717 4372 4559 1700 Size: 110 907 1655 187 2869 Fragments arranged by size: 2869 1655 907 187 110 BsrGI T'GTAC_A Cuts at: 96 3000 96 Size: 2904 2824 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5728 BssSI C'ACGA_G Cuts at: 3788 4800 3788 Size: 1012 4716 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5728 BstBI TT'CG_AA Cuts at: 4532 4532 Size: 5728 BstEII G'GTnAC_C Cuts at: 1184 2720 1184 Size: 1536 4192 BstXI CCAn_nnnn'nTGG Cuts at: 2422 2628 2422 Size: 206 5522 BstYI r'GATC_y Cuts at: 1972 3058 3425 3810 3827 5279 5365 5377 5723 Size: 1086 367 385 17 1452 86 12 346 Cuts at: 5723 1972 Size: 1977 Fragments arranged by size: 1977 1452 1086 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2898 2898 Size: 5728 BtgI C'CryG_G Cuts at: 513 1065 2415 2578 513 Size: 552 1350 163 3663 Fragments arranged by size: 3663 1350 552 163 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2786 487 Size: 683 56 1560 3429 Fragments arranged by size: 3429 1560 683 56 BtsI GCAGTG_nn' Cuts at: 2648 2926 3244 4038 4058 2648 Size: 278 318 794 20 4318 Fragments arranged by size: 4318 794 318 278 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5728 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5693 DraI TTT'AAA Cuts at: 3032 3032 Size: 5728 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4735 809 Size: 507 3419 1802 Fragments arranged by size: 3419 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2063 3175 4064 Size: 54 1425 50 275 18 233 1112 889 Cuts at: 4064 5693 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1112 889 275 233 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3175 1537 Size: 275 1363 4090 Fragments arranged by size: 4090 1363 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2124 2777 2792 3658 1353 Size: 771 653 15 866 3423 Fragments arranged by size: 3423 866 771 653 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4315 4689 4835 387 Size: 1064 138 2726 374 146 1280 Fragments arranged by size: 2726 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2463 2463 Size: 5728 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3791 4388 5175 5443 1239 Size: 393 2159 597 787 268 1524 Fragments arranged by size: 2159 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2058 2111 2292 2411 2525 2567 2751 2058 Size: 53 181 119 114 42 184 5035 Fragments arranged by size: 5035 184 181 119 114 53 42 EcoRI G'AATT_C Cuts at: 3037 3415 3037 Size: 378 5350 EcoRV GAT'ATC Cuts at: 2992 2992 Size: 5728 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2272 2272 Size: 5728 FspI TGC'GCA Cuts at: 4234 4234 Size: 5728 HaeII r_GCGC'y Cuts at: 1678 4875 1678 Size: 3197 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2228 2260 4369 4401 4443 Size: 32 135 32 656 32 2109 32 42 Cuts at: 4443 4475 1373 Size: 32 2626 Fragments arranged by size: 2626 2109 656 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2536 3072 3318 669 Size: 988 281 598 536 246 3079 Fragments arranged by size: 3079 988 598 536 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5728 HpaI GTT'AAC Cuts at: 3318 3318 Size: 5728 KpnI G_GTAC'C Cuts at: 3053 3053 Size: 5728 MfeI C'AATT_G Cuts at: 3327 3327 Size: 5728 MluI A'CGCG_T Cuts at: 5604 5604 Size: 5728 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4842 5026 1305 Size: 3537 184 2007 Fragments arranged by size: 3537 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2065 10 Size: 54 1425 343 233 3673 Fragments arranged by size: 3673 1425 343 233 54 MslI CAynn'nnrTG Cuts at: 518 1166 2235 2626 2736 3686 4045 4204 518 Size: 648 1069 391 110 950 359 159 2042 Fragments arranged by size: 2042 1069 950 648 391 359 159 110 MspA1I CmG'CkG Cuts at: 1606 3144 3821 4969 5214 1606 Size: 1538 677 1148 245 2120 Fragments arranged by size: 2120 1538 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5176 NdeI CA'TA_TG Cuts at: 387 2911 387 Size: 2524 3204 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5728 NotI GC'GGCC_GC Cuts at: 3175 3175 Size: 5728 NspI r_CATG'y Cuts at: 1132 1229 2476 2741 3086 4631 1132 Size: 97 1247 265 345 1545 2229 Fragments arranged by size: 2229 1545 1247 345 265 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5728 PciI A'CATG_T Cuts at: 2472 4627 2472 Size: 2155 3573 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5728 PfoI T'CCnGG_A Cuts at: 2456 2456 Size: 5728 PmeI GTTT'AAAC Cuts at: 3032 3032 Size: 5728 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3797 3829 5310 5342 3797 Size: 32 1481 32 4183 Fragments arranged by size: 4183 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2111 2292 2751 2111 Size: 181 459 5088 Fragments arranged by size: 5088 459 181 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5728 PsiI TTA'TAA Cuts at: 3298 3298 Size: 5728 PspOMI G'GGCC_C Cuts at: 2410 2525 2566 2410 Size: 115 41 5572 Fragments arranged by size: 5572 115 41 PspXI vC'TCGA_Gb Cuts at: 2638 2638 Size: 5728 PstI C_TGCA'G Cuts at: 830 2055 2453 2524 2854 3080 830 Size: 1225 398 71 330 226 3478 Fragments arranged by size: 3478 1225 398 330 226 71 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5728 SacI G_AGCT'C Cuts at: 2465 2465 Size: 5728 SalI G'TCGA_C Cuts at: 1936 3070 1936 Size: 1134 4594 SbfI CC_TGCA'GG Cuts at: 2854 3080 2854 Size: 226 5502 ScaI AGT'ACT Cuts at: 3976 5688 3976 Size: 1712 4016 SfcI C'TryA_G Cuts at: 826 1045 2051 2296 2449 2520 2590 2850 3076 Size: 219 1006 245 153 71 70 260 226 Cuts at: 3076 4211 4892 5083 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1006 681 260 245 226 219 191 153 71 70 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5728 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5728 SmaI CCC'GGG Cuts at: 1872 3055 1872 Size: 1183 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2638 3832 4733 4995 820 Size: 197 926 695 1194 901 262 1553 Fragments arranged by size: 1553 1194 926 901 695 262 197 SnaBI TAC'GTA Cuts at: 493 3519 493 Size: 3026 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5728 SphI G_CATG'C Cuts at: 1132 3086 1132 Size: 1954 3774 SspI AAT'ATT Cuts at: 5 52 3652 5 Size: 47 3600 2081 Fragments arranged by size: 3600 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5728 StyI C'CwwG_G Cuts at: 513 1065 2247 2529 3167 513 Size: 552 1182 282 638 3074 Fragments arranged by size: 3074 1182 638 552 282 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2121 2255 2591 2710 3755 3772 3925 Size: 1255 541 134 336 119 1045 17 153 Cuts at: 3925 4110 4555 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1045 541 445 336 185 153 134 119 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3000 3974 5686 Size: 274 80 33 51 1443 1023 974 1712 Cuts at: 5686 96 Size: 138 Fragments arranged by size: 1712 1443 1023 974 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2421 2453 2995 3027 5688 5720 2421 Size: 32 542 32 2661 32 2429 Fragments arranged by size: 2661 2429 542 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2363 2443 1316 Size: 1047 80 4601 Fragments arranged by size: 4601 1047 80 XbaI T'CTAG_A Cuts at: 3064 3064 Size: 5728 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2353 2011 Size: 342 5386 XhoI C'TCGA_G Cuts at: 1943 2638 1943 Size: 695 5033 XmaI C'CCGG_G Cuts at: 1870 3053 1870 Size: 1183 4545 XmnI GAAnn'nnTTC Cuts at: 2089 2674 3857 2089 Size: 585 1183 3960 Fragments arranged by size: 3960 1183 585 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3536 276 Size: 53 83 186 936 2002 2468 Fragments arranged by size: 2468 2002 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI