Restriction map for
pFN21AE3920
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FauI
FbaI FokI FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KpnI Ksp632I KspI MaeII MamI
MflI MluI MroI MslI MspA1I
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TfiI Tth111I TthHB8I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 151 GTAGAC GT^[AC][GT]AC
AfaI 334 GTAC GT^AC
AlwNI 10 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 748 GTGCTC G[AT]GC[AT]^C
BbsI 700 GAAGAC GAAGAC(2/6)
BbvI 386 GCAGC GCAGC(8/12)
BmyI 748 GTGCTC G[AGT]GC[ACT]^C
BpmI 569 CTGGAG CTGGAG(16/14)
BpuAI 700 GAAGAC GAAGAC(2/6)
BsaI 113 GAGACC GGTCTC(1/5)
BsaJI 630 CCTCGG C^C[ACGT][ACGT]GG
BseRI 473 CTCCTC GAGGAG(10/8)
BsiHKAI 748 GTGCTC G[AT]GC[AT]^C
BsmI 712 GAATGC GAATGC(1/-1)
Bsp1286I 748 GTGCTC G[AGT]GC[ACT]^C
Cac8I 94 GCACGC GC[ACGT]^[ACGT]GC
CfoI 445 GCGC GCG^C
DpnII 286 GATC ^GATC
EcoRI 121 GAATTC G^AATTC
Fnu4HI 386 GCAGC GC^[ACGT]GC
HaeII 444 AGCGCC [GA]GCGC^[CT]
HapII 348 CCGG C^CGG
HhaI 445 GCGC GCG^C
HinP1I 445 GCGC G^CGC
HindIII 359 AAGCTT A^AGCTT
HinfI 572 GAGTC G^A[ACGT]TC
HpaII 348 CCGG C^CGG
ItaI 386 GCAGC GC^[ACGT]GC
MboI 286 GATC ^GATC
MfeI 395 CAATTG C^AATTG
MspI 348 CCGG C^CGG
MunI 395 CAATTG C^AATTG
MwoI 380 GCTCTTGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 286 GATC ^GATC
NspI 98 GCATGT [GA]CATG^[CT]
PflMI 519 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 572 GAGTC GAGTC(4/5)
RsaI 334 GTAC GT^AC
Sau3AI 286 GATC ^GATC
SexAI 524 ACCTGGT A^CC[AT]GGT
SfaNI 402 GATGC GCATC(5/9)
SmlI 356 CTCAAG C^T[CT][GA]AG
TseI 386 GCAGC G^C[AT]GC
Tsp45I 19 GTCAC ^GT[GC]AC
Van91I 519 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcsI 121 GAATTC [GA]^AATT[CT]
223 GAATTT
ApoI 121 GAATTC [GA]^AATT[CT]
223 GAATTT
Asp700I 39 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
661 GAAGGCTTTC
BalI 138 TGGCCA TGG^CCA
611 TGGCCA
BfaI 280 CTAG C^TAG
683 CTAG
BsiYI 519 CCATGACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
603 CCGAAAATTGG
BslI 519 CCATGACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
603 CCGAAAATTGG
BsmAI 113 GAGAC GTCTC(1/5)
778 GAGAC
BspMI 302 ACCTGC ACCTGC(4/8)
318 ACCTGC
BssKI 452 CCTGG ^CC[ACGT]GG
525 CCTGG
BstNI 452 CCTGG CC^[AT]GG
525 CCTGG
EaeI 138 TGGCCA [CT]^GGCC[GA]
611 TGGCCA
EcoRII 452 CCTGG ^CC[AT]GG
525 CCTGG
HaeIII 139 GGCC GG^CC
612 GGCC
HphI 163 GGTGA GGTGA(8/7)
201 TCACC
MaeI 280 CTAG C^TAG
683 CTAG
MaeIII 19 GTCAC ^GT[ACGT]AC
461 GTAAC
MluNI 138 TGGCCA TGG^CCA
611 TGGCCA
MscI 138 TGGCCA TGG^CCA
611 TGGCCA
MseI 292 TTAA T^TAA
655 TTAA
MvaI 452 CCTGG CC^[AT]GG
525 CCTGG
NlaIII 99 CATG CATG^
520 CATG
ScrFI 452 CCTGG CC^[ACGT]GG
525 CCTGG
SfcI 307 CTACAG C^T[GA][CT]AG
717 CTATAG
Tru9I 292 TTAA T^TAA
655 TTAA
XmnI 39 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
661 GAAGGCTTTC
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# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
AluI BsrI DdeI MboII MnlI
Tsp509I TspRI