Restriction map for
pFN21AE1408
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII AccII AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DdeI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI Ksp632I KspI MaeI
MaeIII MamI MfeI MflI MluI
MroI MseI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tru9I Tsp45I Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 93 GGTACC G^GTACC
AccI 430 GTCTAC GT^[AC][GT]AC
AfaI 94 GTAC GT^AC
AflIII 102 ACATGT A^C[GA][CT]GT
Aor51HI 134 AGCGCT AGC^GCT
Asp718I 93 GGTACC G^GTACC
AspHI 346 GAGCTC G[AT]GC[AT]^C
AvaII 224 GGACC G^G[AT]CC
BalI 15 TGGCCA TGG^CCA
BanI 93 GGTACC G^G[CT][GA]CC
BanII 346 GAGCTC G[GA]GC[CT]^C
BsiHKAI 346 GAGCTC G[AT]GC[AT]^C
BsiYI 256 CCCGAAGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 256 CCCGAAGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 314 GAGAC GTCTC(1/5)
BspLU11I 102 ACATGT A^CATGT
BspMI 146 ACCTGC ACCTGC(4/8)
BsrI 127 CCAGT ACTGG(1/-1)
BstF5I 249 GGATG GGATG(2/0)
Cac8I 116 GCCCGC GC[ACGT]^[ACGT]GC
CfoI 135 GCGC GCG^C
DpnII 389 GATC ^GATC
DraII 223 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 219 CCATGG C^C[GA][CT]GG
Eco47III 134 AGCGCT AGC^GCT
EcoO109I 223 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 219 CCATGG C^C[AT][AT]GG
FokI 249 GGATG GGATG(9/13)
HaeII 134 AGCGCT [GA]GCGC^[CT]
HapII 263 CCGG C^CGG
HgaI 150 GCGTC GACGC(5/10)
HhaI 135 GCGC GCG^C
HinP1I 135 GCGC G^CGC
HinfI 191 GAGTC G^A[ACGT]TC
HpaII 263 CCGG C^CGG
HphI 233 TCACC GGTGA(8/7)
KpnI 93 GGTACC GGTAC^C
MboI 389 GATC ^GATC
MboII 297 TCTTC GAAGA(8/7)
MluNI 15 TGGCCA TGG^CCA
MscI 15 TGGCCA TGG^CCA
MspA1I 118 CCGCTG C[AC]G^C[GT]G
MspI 263 CCGG C^CGG
NcoI 219 CCATGG C^CATGG
NdeII 389 GATC ^GATC
NspI 102 ACATGT [GA]CATG^[CT]
PleI 191 GAGTC GAGTC(4/5)
PpuMI 223 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 145 GACCTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 94 GTAC GT^AC
SacI 346 GAGCTC GAGCT^C
Sau3AI 389 GATC ^GATC
SfaNI 335 GCATC GCATC(5/9)
SfcI 38 CTGTAG C^T[GA][CT]AG
StyI 219 CCATGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AluI 347 AGCT AG^CT
420 AGCT
BmyI 114 GTGCCC G[AGT]GC[ACT]^C
346 GAGCTC
BpmI 349 CTCCAG CTGGAG(16/14)
311 CTGGAG
BsaJI 219 CCATGG C^C[ACGT][ACGT]GG
301 CCCAGG
BseRI 343 GAGGAG GAGGAG(10/8)
394 GAGGAG
BsmFI 196 GGGAC GGGAC(10/14)
223 GGGAC
Bsp1286I 114 GTGCCC G[AGT]GC[ACT]^C
346 GAGCTC
BssKI 97 CCAGG ^CC[ACGT]GG
302 CCAGG
BstNI 97 CCAGG CC^[AT]GG
302 CCAGG
Cfr13I 224 GGACC G^G[ACGT]CC
253 GGCCC
EcoRII 97 CCAGG ^CC[AT]GG
302 CCAGG
FauI 117 CCCGC CCCGC(4/6)
426 GCGGG
MaeII 281 ACGT A^CGT
287 ACGT
MvaI 97 CCAGG CC^[AT]GG
302 CCAGG
Sau96I 224 GGACC G^G[ACGT]CC
253 GGCCC
ScrFI 97 CCAGG CC^[ACGT]GG
302 CCAGG
TspRI 128 CAGTG CA[GC]TG[ACGT][ACGT]^
200 CAGTG
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# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
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AciI BbvI EaeI Fnu4HI HaeIII
ItaI MnlI MslI MwoI NlaIII
NlaIV TseI