################################## # NO CUT ENZYMES ( 202 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaJI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII HphI KasI KpnI KspI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StuI StyI SwaI Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AciI 445 CCGC CCGC(-3/-1) Asp700I 145 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbsI 595 GAAGAC GAAGAC(2/6) BbvI 493 GCTGC GCAGC(8/12) BfaI 367 CTAG C^TAG BpuAI 595 GAAGAC GAAGAC(2/6) BsaI 392 GAGACC GGTCTC(1/5) BsgI 23 GTGCAG GTGCAG(16/14) BsmAI 392 GAGAC GTCTC(1/5) BssKI 362 CCAGG ^CC[ACGT]GG BstNI 362 CCAGG CC^[AT]GG Cfr13I 536 GGGCC G^G[ACGT]CC EaeI 336 TGGCCG [CT]^GGCC[GA] EcoRI 587 GAATTC G^AATTC EcoRII 362 CCAGG ^CC[AT]GG Fnu4HI 493 GCTGC GC^[ACGT]GC HincII 178 GTTGAC GT[CT]^[GA]AC HindII 178 GTTGAC GT[CT]^[GA]AC HindIII 156 AAGCTT A^AGCTT HinfI 268 GAATC G^A[ACGT]TC ItaI 493 GCTGC GC^[ACGT]GC MaeI 367 CTAG C^TAG MseI 85 TTAA T^TAA MvaI 362 CCAGG CC^[AT]GG NheI 366 GCTAGC G^CTAGC NspI 297 GCATGC [GA]CATG^[CT] Sau96I 536 GGGCC G^G[ACGT]CC ScrFI 362 CCAGG CC^[ACGT]GG SfcI 307 CTACAG C^T[GA][CT]AG SphI 297 GCATGC GCATG^C SspI 54 AATATT AAT^ATT TaqI 390 TCGA T^CGA TfiI 268 GAATC G^A[AT]TC Tru9I 85 TTAA T^TAA TseI 493 GCTGC G^C[AT]GC TthHB8I 390 TCGA T^CGA XmnI 145 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AcsI 547 AAATTT [GA]^AATT[CT] 587 GAATTC AlwI 442 GATCC GGATC(4/5) 191 GGATC ApoI 547 AAATTT [GA]^AATT[CT] 587 GAATTC DpnII 192 GATC ^GATC 442 GATC EarI 233 CTCTTC CTCTTC(1/4) 352 GAAGAG HaeIII 337 GGCC GG^CC 537 GGCC Ksp632I 233 CTCTTC CTCTTC(1/4) 352 GAAGAG MboI 192 GATC ^GATC 442 GATC NdeII 192 GATC ^GATC 442 GATC SapI 352 GAAGAGC GCTCTTC(1/4) 232 GCTCTTC Sau3AI 192 GATC ^GATC 442 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 8 enzymes) # ######################################### AluI Cac8I DdeI MboII MnlI MwoI NlaIII Tsp509I