Restriction map for
pFN21AB3356
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AlwNI AocI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaII AviII BalI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfrI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI Bsu36I CelII Cfr10I ClaI
CpoI DraII DrdI EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HapII HgaI Hin1I
HpaI HpaII KasI KpnI MamI
MluI MluNI MroI MscI MspI
NaeI NarI NciI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 533 GTATAC GT^[AC][GT]AC
AccII 32 CGCG CG^CG
AflIII 772 ACATGT A^C[GA][CT]GT
Alw44I 2183 GTGCAC G^TGCAC
Aor51HI 2480 AGCGCT AGC^GCT
ApaLI 2183 GTGCAC G^TGCAC
AvaI 2099 CCCGAG C^[CT]CG[GA]G
AvrII 741 CCTAGG C^CTAGG
BamHI 295 GGATCC G^GATCC
BglI 1316 GCCCAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 593 AGATCT A^GATCT
BlnI 741 CCTAGG C^CTAGG
BsaI 1860 GGTCTC GGTCTC(1/5)
BsmI 1077 GAATGC GAATGC(1/-1)
BsoBI 2099 CCCGAG C^[CT]CG[GA]G
BspLU11I 772 ACATGT A^CATGT
Bst1107I 533 GTATAC GTA^TAC
BstUI 32 CGCG CG^CG
BstZ17I 533 GTATAC GTA^TAC
CfoI 2481 GCGC GCG^C
Cfr13I 2470 GGCCC G^G[ACGT]CC
DraIII 167 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2480 AGCGCT AGC^GCT
EcoNI 1780 CCTATGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 2480 AGCGCT [GA]GCGC^[CT]
HhaI 2481 GCGC GCG^C
HinP1I 2481 GCGC G^CGC
KspI 31 CCGCGG CCGC^GG
MspA1I 31 CCGCGG C[AC]G^C[GT]G
MvnI 32 CGCG CG^CG
NcoI 1129 CCATGG C^CATGG
NheI 1737 GCTAGC G^CTAGC
PflMI 769 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 31 CCGCGG CCGC^GG
Sau96I 2470 GGCCC G^G[ACGT]CC
SexAI 856 ACCTGGT A^CC[AT]GGT
SfaNI 1999 GCATC GCATC(5/9)
SfcI 225 CTGTAG C^T[GA][CT]AG
SmlI 1415 CTCAAG C^T[CT][GA]AG
TaqI 2463 TCGA T^CGA
TthHB8I 2463 TCGA T^CGA
Van91I 769 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 442 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 1249 GAATTT [GA]^AATT[CT]
1362 AAATTT
ApoI 1249 GAATTT [GA]^AATT[CT]
1362 AAATTT
Asp700I 1246 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1684 GAAGATTTTC
BclI 504 TGATCA T^GATCA
876 TGATCA
BseRI 1634 CTCCTC GAGGAG(10/8)
964 GAGGAG
BsmFI 1885 GTCCC GGGAC(10/14)
2035 GTCCC
BstXI 538 CCAGAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1107 CCAAGTAGATGG
DraI 708 TTTAAA TTT^AAA
910 TTTAAA
EcoT14I 741 CCTAGG C^C[AT][AT]GG
1129 CCATGG
FbaI 504 TGATCA T^GATCA
876 TGATCA
HindIII 979 AAGCTT A^AGCTT
1549 AAGCTT
NspI 772 ACATGT [GA]CATG^[CT]
2071 GCATGT
SspI 343 AATATT AAT^ATT
791 AATATT
StyI 741 CCTAGG C^C[AT][AT]GG
1129 CCATGG
Tsp45I 2131 GTGAC ^GT[GC]AC
2168 GTCAC
XmnI 1246 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1684 GAAGATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BbvI BfaI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I DdeI DpnII DsaI
EarI EcoRII Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MunI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
ScrFI TfiI Tru9I TseI Tsp509I
TspRI XhoII