(Circular) (Six-base) MAPSORT of: pFN21AB3356.seq Check: 6514 from: 1 to: 7282 pFN21AB3356 7282 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5092 278 Size: 53 83 186 936 3556 2468 Fragments arranged by size: 3556 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2539 4625 1937 Size: 602 2086 4594 Fragments arranged by size: 4594 2086 602 Acc65I G'GTAC_C Cuts at: 4603 4603 Size: 7282 AclI AA'CG_TT Cuts at: 1563 5409 5782 1563 Size: 3846 373 3063 Fragments arranged by size: 3846 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3756 4338 5345 6729 6997 1632 Size: 2124 582 1007 1384 268 1917 Fragments arranged by size: 2124 1917 1384 1007 582 268 AfeI AGC'GCT Cuts at: 4487 4487 Size: 7282 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7085 AflIII A'CryG_T Cuts at: 1203 2777 6181 7158 1203 Size: 1574 3404 977 1327 Fragments arranged by size: 3404 1574 1327 977 AgeI A'CCGG_T Cuts at: 6113 6113 Size: 7282 AhdI GACnn_n'nnGTC Cuts at: 6011 6011 Size: 7282 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7282 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7282 AlwNI CAG_nnn'CTG Cuts at: 5025 6597 5025 Size: 1572 5710 ApaLI G'TGCA_C Cuts at: 4188 5339 6495 4188 Size: 1151 1156 4975 Fragments arranged by size: 4975 1156 1151 ApoI r'AATT_y Cuts at: 1493 3254 3367 4591 4817 4969 7078 1493 Size: 1761 113 1224 226 152 2109 1697 Fragments arranged by size: 2109 1761 1697 1224 226 152 113 AseI AT'TA_AT Cuts at: 160 1032 5836 160 Size: 872 4804 1606 Fragments arranged by size: 4804 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7282 AvaI C'yCGr_G Cuts at: 1870 1943 4104 4607 1870 Size: 73 2161 503 4545 Fragments arranged by size: 4545 2161 503 73 AvrII C'CTAG_G Cuts at: 2746 2746 Size: 7282 BamHI G'GATC_C Cuts at: 2300 4612 4979 2300 Size: 2312 367 4603 Fragments arranged by size: 4603 2312 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4603 6058 618 Size: 325 217 627 2816 1455 1842 Fragments arranged by size: 2816 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4601 1391 Size: 3210 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6311 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5473 5507 1903 Size: 34 3536 34 3678 Fragments arranged by size: 3678 3536 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5174 6384 5174 Size: 1210 6072 BclI T'GATC_A Cuts at: 1548 2509 2881 1548 Size: 961 372 5949 Fragments arranged by size: 5949 961 372 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3327 5893 136 Size: 107 122 71 631 605 1655 2566 1525 Fragments arranged by size: 2566 1655 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2598 7277 2598 Size: 4679 2603 BlpI GC'TnA_GC Cuts at: 4699 4699 Size: 7282 Bme1580I G_kGCm'C Cuts at: 1790 4192 5343 6499 1790 Size: 2402 1151 1156 2573 Fragments arranged by size: 2573 2402 1156 1151 BmgBI CAC'GTC Cuts at: 1318 3842 4172 1318 Size: 2524 330 4428 Fragments arranged by size: 4428 2524 330 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3169 3455 5013 Size: 416 53 83 399 24 1752 286 1558 Cuts at: 5013 5971 442 Size: 958 1753 Fragments arranged by size: 1753 1752 1558 958 416 399 286 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3746 1056 Size: 2690 4592 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2038 2139 4363 4470 5942 1239 Size: 799 101 2224 107 1472 2579 Fragments arranged by size: 2579 2224 1472 799 107 101 Bpu10I CC'TnA_GC Cuts at: 3666 3666 Size: 7282 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3405 5407 6272 6570 3405 Size: 2002 865 298 4117 Fragments arranged by size: 4117 2002 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3871 5945 882 Size: 617 2372 2074 2219 Fragments arranged by size: 2372 2219 2074 617 BsaAI yAC'GTr Cuts at: 493 1204 5073 493 Size: 711 3869 2702 Fragments arranged by size: 3869 2702 711 BsaBI GATnn'nnATC Cuts at: 7276 7276 Size: 7282 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5089 5471 6106 275 Size: 53 83 186 936 3556 382 635 1451 Fragments arranged by size: 3556 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5715 6113 6387 6534 6945 5715 Size: 398 274 147 411 6052 Fragments arranged by size: 6052 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3870 3900 1178 Size: 30 165 30 2467 30 4560 Fragments arranged by size: 4560 2467 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2984 3630 1186 Size: 1798 646 4838 Fragments arranged by size: 4838 1798 646 BseYI C'CCAG_C Cuts at: 2072 4218 6485 6981 2072 Size: 2146 2267 496 2373 Fragments arranged by size: 2373 2267 2146 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6709 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4732 5493 6521 1540 Size: 275 186 2731 761 1028 2301 Fragments arranged by size: 2731 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2024 2095 2173 4192 5343 5428 6499 2024 Size: 71 78 2019 1151 85 1071 2807 Fragments arranged by size: 2807 2019 1151 1071 85 78 71 BsmI GAATG_Cn' Cuts at: 3088 4793 4886 3088 Size: 1705 93 5484 Fragments arranged by size: 5484 1705 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2095 2173 4192 4601 5343 5428 Size: 399 234 71 78 2019 409 742 85 Cuts at: 5428 6499 1391 Size: 1071 2174 Fragments arranged by size: 2174 2019 1071 742 409 399 234 85 78 71 BspEI T'CCGG_A Cuts at: 6945 6945 Size: 7282 BspHI T'CATG_A Cuts at: 5171 6901 5171 Size: 1730 5552 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4637 844 Size: 3793 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5169 1394 Size: 429 3346 3507 Fragments arranged by size: 3507 3346 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2792 3202 3316 5777 5951 65 Size: 1183 1544 410 114 2461 174 1396 Fragments arranged by size: 2461 1544 1396 1183 410 174 114 BsrFI r'CCGG_y Cuts at: 1700 1810 5926 6113 1700 Size: 110 4116 187 2869 Fragments arranged by size: 4116 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7282 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7282 BssSI C'ACGA_G Cuts at: 5342 6354 5342 Size: 1012 6270 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7282 BstBI TT'CG_AA Cuts at: 6086 6086 Size: 7282 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7282 BstXI CCAn_nnnn'nTGG Cuts at: 2550 3119 2550 Size: 569 6713 BstYI r'GATC_y Cuts at: 1972 2300 2598 4349 4612 4979 5364 5381 6833 Size: 328 298 1751 263 367 385 17 1452 Cuts at: 6833 6919 6931 7277 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1751 1452 385 367 346 328 298 263 86 17 12 BstZ17I GTA'TAC Cuts at: 2540 2540 Size: 7282 BtgI C'CryG_G Cuts at: 513 1065 2004 2036 3134 3951 513 Size: 552 939 32 1098 817 3844 Fragments arranged by size: 3844 1098 939 817 552 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3459 487 Size: 683 56 2233 4310 Fragments arranged by size: 4310 2233 683 56 BtsI GCAGTG_nn' Cuts at: 3486 4503 4798 5592 5612 3486 Size: 1017 295 794 20 5156 Fragments arranged by size: 5156 1017 794 295 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7282 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7247 DraI TTT'AAA Cuts at: 2715 2917 4586 2715 Size: 202 1669 5411 Fragments arranged by size: 5411 1669 202 DraIII CAC_nnn'GTG Cuts at: 2177 2177 Size: 7282 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6289 809 Size: 507 4973 1802 Fragments arranged by size: 4973 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4729 5618 7247 Size: 54 1425 50 275 18 2899 889 1629 Cuts at: 7247 8 Size: 43 Fragments arranged by size: 2899 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4729 1537 Size: 275 2917 4090 Fragments arranged by size: 4090 2917 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2404 2589 3625 3982 4297 4392 5212 1353 Size: 1051 185 1036 357 315 95 820 3423 Fragments arranged by size: 3423 1051 1036 820 357 315 185 95 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5869 6243 6389 387 Size: 1064 138 4280 374 146 1280 Fragments arranged by size: 4280 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2038 2139 3756 4338 4363 4470 5345 Size: 393 406 101 1617 582 25 107 875 Cuts at: 5345 5942 6729 6997 1239 Size: 597 787 268 1524 Fragments arranged by size: 1617 1524 875 787 597 582 406 393 268 107 101 25 EcoNI CCTnn'n_nnAGG Cuts at: 3789 3789 Size: 7282 EcoRI G'AATT_C Cuts at: 4591 4969 4591 Size: 378 6904 FspI TGC'GCA Cuts at: 5788 5788 Size: 7282 HaeII r_GCGC'y Cuts at: 1678 4489 6429 1678 Size: 2811 1940 2531 Fragments arranged by size: 2811 2531 1940 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2429 2461 4385 4417 5923 Size: 32 135 32 857 32 1924 32 1506 Cuts at: 5923 5955 5997 6029 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1924 1506 857 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2136 2328 3000 4626 4872 669 Size: 988 281 198 192 672 1626 246 3079 Fragments arranged by size: 3079 1626 988 672 281 246 198 192 HindIII A'AGCT_T Cuts at: 748 2984 3554 748 Size: 2236 570 4476 Fragments arranged by size: 4476 2236 570 HpaI GTT'AAC Cuts at: 4872 4872 Size: 7282 KpnI G_GTAC'C Cuts at: 4607 4607 Size: 7282 MfeI C'AATT_G Cuts at: 2854 3229 3656 4881 2854 Size: 375 427 1225 5255 Fragments arranged by size: 5255 1225 427 375 MluI A'CGCG_T Cuts at: 7158 7158 Size: 7282 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2648 6396 6580 1305 Size: 1343 3748 184 2007 Fragments arranged by size: 3748 2007 1343 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5460 Fragments arranged by size: 5460 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2433 3066 3541 5240 5599 5758 518 Size: 648 1267 633 475 1699 359 159 2042 Fragments arranged by size: 2042 1699 1267 648 633 475 359 159 MspA1I CmG'CkG Cuts at: 1606 2038 4698 5375 6523 6768 1606 Size: 432 2660 677 1148 245 2120 Fragments arranged by size: 2660 2120 1148 677 432 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3134 513 Size: 552 939 1130 4661 Fragments arranged by size: 4661 1130 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7282 NheI G'CTAG_C Cuts at: 1052 3742 1052 Size: 2690 4592 NotI GC'GGCC_GC Cuts at: 4729 4729 Size: 7282 NspI r_CATG'y Cuts at: 1132 1229 2781 4080 4640 6185 1132 Size: 97 1552 1299 560 1545 2229 Fragments arranged by size: 2229 1552 1545 1299 560 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7282 PciI A'CATG_T Cuts at: 2777 6181 2777 Size: 3404 3878 PflMI CCAn_nnn'nTGG Cuts at: 1108 2780 1108 Size: 1672 5610 PfoI T'CCnGG_A Cuts at: 2845 4447 2845 Size: 1602 5680 PmeI GTTT'AAAC Cuts at: 4586 4586 Size: 7282 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5351 5383 6864 6896 5351 Size: 32 1481 32 5737 Fragments arranged by size: 5737 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7282 PsiI TTA'TAA Cuts at: 4852 4852 Size: 7282 PstI C_TGCA'G Cuts at: 830 4634 830 Size: 3804 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7282 SacII CC_GC'GG Cuts at: 2039 2039 Size: 7282 SalI G'TCGA_C Cuts at: 1936 4624 1936 Size: 2688 4594 SbfI CC_TGCA'GG Cuts at: 4634 4634 Size: 7282 ScaI AGT'ACT Cuts at: 5530 7242 5530 Size: 1712 5570 SexAI A'CCwGG_T Cuts at: 2861 2861 Size: 7282 SfcI C'TryA_G Cuts at: 826 1045 2230 4630 5765 6446 6637 826 Size: 219 1185 2400 1135 681 191 1471 Fragments arranged by size: 2400 1471 1185 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7282 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7282 SmaI CCC'GGG Cuts at: 1872 4609 1872 Size: 2737 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3420 5386 6287 6549 820 Size: 197 926 1477 1966 901 262 1553 Fragments arranged by size: 1966 1553 1477 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5073 493 Size: 4580 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7282 SphI G_CATG'C Cuts at: 1132 4640 1132 Size: 3508 3774 SspI AAT'ATT Cuts at: 5 52 2350 2798 5206 5 Size: 47 2298 448 2408 2081 Fragments arranged by size: 2408 2298 2081 448 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7282 StyI C'CwwG_G Cuts at: 513 1065 2004 2746 3134 4721 513 Size: 552 939 742 388 1587 3074 Fragments arranged by size: 3074 1587 939 742 552 388 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3397 5309 5326 5479 5664 6109 325 Size: 1255 1817 1912 17 153 185 445 1498 Fragments arranged by size: 1912 1817 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3545 5528 7240 Size: 274 80 33 51 1443 1568 1983 1712 Cuts at: 7240 96 Size: 138 Fragments arranged by size: 1983 1712 1568 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4119 4151 7242 7274 4119 Size: 32 3091 32 4127 Fragments arranged by size: 4127 3091 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7282 XbaI T'CTAG_A Cuts at: 2447 4618 2447 Size: 2171 5111 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7282 XmaI C'CCGG_G Cuts at: 1870 4607 1870 Size: 2737 4545 XmnI GAAnn'nnTTC Cuts at: 3255 3693 5411 3255 Size: 438 1718 5126 Fragments arranged by size: 5126 1718 438 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5090 276 Size: 53 83 186 936 3556 2468 Fragments arranged by size: 3556 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI Bsu36I EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI XcmI