################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AflIII Alw44I Aor51HI ApaLI AscI Asp718I AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsgI BsiWI BsmBI BsoBI BspDI BspLU11I BsrBI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccIII 439 TCCGGA T^CCGGA AgeI 1472 ACCGGT A^CCGGT AhdI 1147 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 54 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 831 CCTGAGG CC^T[ACGT]AGG ApaI 1422 GGGCCC GGGCC^C AseI 1225 ATTAAT AT^TAAT AsnI 1225 ATTAAT AT^TAAT Asp700I 909 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1147 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 859 GAGCTC G[AT]GC[AT]^C BbsI 1207 GAAGAC GAAGAC(2/6) BclI 632 TGATCA T^GATCA BcnI 666 CCGGG CC^[GC]GG BfaI 155 CTAG C^TAG BpuAI 1207 GAAGAC GAAGAC(2/6) BseAI 439 TCCGGA T^CCGGA BsiEI 1474 CGGTCG CG[GA][CT]^CG BsiHKAI 859 GAGCTC G[AT]GC[AT]^C Bsp1407I 764 TGTACA T^GTACA BspEI 439 TCCGGA T^CCGGA BspHI 629 TCATGA T^CATGA BspMI 85 ACCTGC ACCTGC(4/8) BsrFI 1472 ACCGGT [GA]^CCGG[CT] BsrGI 764 TGTACA T^GTACA BssSI 1496 CACGAG CACGAG(-5/-1) Bsu36I 831 CCTGAGG CC^T[ACGT]AGG CfoI 1248 GCGC GCG^C Cfr10I 1472 ACCGGT [GA]^CCGG[CT] DraII 113 GGGGCCT [GA]G^G[ACGT]CC[CT] Eam1105I 1147 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 831 CCTGAGG CC^T[ACGT]AGG EcoNI 47 CCTCCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 113 GGGGCCT [GA]G^G[ACGT]CC[CT] FauI 1157 CCCGC CCCGC(4/6) FbaI 632 TGATCA T^GATCA HhaI 1248 GCGC GCG^C HinP1I 1248 GCGC G^CGC HphI 657 GGTGA GGTGA(8/7) MaeI 155 CTAG C^TAG MaeII 905 ACGT A^CGT MroI 439 TCCGGA T^CCGGA MslI 1095 CATAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1158 CCGCTG C[AC]G^C[GT]G NciI 666 CCGGG CC^[GC]GG NheI 154 GCTAGC G^CTAGC NspI 695 ACATGC [GA]CATG^[CT] PinAI 1472 ACCGGT A^CCGGT PshBI 1225 ATTAAT AT^TAAT RcaI 629 TCATGA T^CATGA SacI 859 GAGCTC GAGCT^C SapI 96 GAAGAGC GCTCTTC(1/4) SexAI 534 ACCAGGT A^CC[AT]GGT SspBI 764 TGTACA T^GTACA TaqI 711 TCGA T^CGA Tsp45I 706 GTCAC ^GT[GC]AC TthHB8I 711 TCGA T^CGA XcmI 1364 CCAAATTGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 909 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AciI 612 CCGC CCGC(-3/-1) 1158 CCGC AlwI 258 GATCC GGATC(4/5) 1360 GATCC AvaII 947 GGACC G^G[AT]CC 1470 GGACC BciVI 328 GTATCC GTATCC(6/5) 453 GTATCC BpmI 1150 CTCCAG CTGGAG(16/14) 76 CTGGAG BsaI 922 GAGACC GGTCTC(1/5) 1270 GAGACC BsaWI 439 TCCGGA [AT]^CCGG[AT] 1472 ACCGGT BsmI 780 GAATGC GAATGC(1/-1) 872 GCATTC BsrDI 273 CATTGC GCAATG(2/0) 101 GCAATG BstXI 1116 CCAGTCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1426 CCAGGGCAGTGG BstYI 257 AGATCC [GA]^GATC[CT] 1359 AGATCC Cac8I 154 GCTAGC GC[ACGT]^[ACGT]GC 868 GCAAGC DdeI 607 CTCAG C^T[ACGT]AG 832 CTGAG DsaI 1342 CCATGG C^C[GA][CT]GG 1373 CCATGG EcoRI 579 GAATTC G^AATTC 913 GAATTC MaeIII 379 GTTAC ^GT[ACGT]AC 706 GTCAC MflI 257 AGATCC [GA]^GATC[CT] 1359 AGATCC NcoI 1342 CCATGG C^CATGG 1373 CCATGG SfaNI 421 GATGC GCATC(5/9) 813 GATGC SmlI 727 CTCAAG C^T[CT][GA]AG 1081 CTTGAG TfiI 406 GAATC G^A[AT]TC 788 GATTC XhoII 257 AGATCC [GA]^GATC[CT] 1359 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AcsI AfaI AluI ApoI BalI BanII BbvI BmyI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DpnII DraI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII ItaI Ksp632I MboI MboII MluNI MnlI MscI MseI MspI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI StyI Tru9I TseI Tsp509I TspRI