Restriction map for pFN21AE4510
 (Circular) (Six-base) MAPSORT of: pFN21AE4510.seq  Check: 6576  from: 1  to: 6301



pFN21AE4510  6301 bp

 With 182 enzymes: SgfI * 

                        July 6, 2012 17:43  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2099    2099 
   Size:       6301
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4111     278 
   Size:         53      83     186     936    2575    2468

  Fragments arranged by size: 

                2575    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3644    1937 
   Size:       1707    4594
 
Acc65I G'GTAC_C

Cuts at:   3622    3622 
   Size:       6301
 
AclI AA'CG_TT

Cuts at:   1563    4428    4801    1563 
   Size:       2865     373    3063

  Fragments arranged by size: 

                3063    2865     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2327    3503    3569    4364    5748    6016    1632 
   Size:        695    1176      66     795    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1176     795     695     268      66
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6104
 
AflIII A'CryG_T

Cuts at:   1203    5200    6177    1203 
   Size:       3997     977    1327

  Fragments arranged by size: 

                3997    1327     977
 
AgeI A'CCGG_T

Cuts at:   3477    5132    3477 
   Size:       1655    4646
 
AhdI GACnn_n'nnGTC

Cuts at:   3157    5030    3157 
   Size:       1873    4428
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6301
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2802    1184 
   Size:       1618    4683
 
AlwNI CAG_nnn'CTG

Cuts at:   2064    4044    5616    2064 
   Size:       1980    1572    2749

  Fragments arranged by size: 

                2749    1980    1572
 
ApaI G_GGCC'C

Cuts at:   3431    3431 
   Size:       6301
 
ApaLI G'TGCA_C

Cuts at:   4358    5514    4358 
   Size:       1156    5145
 
ApoI r'AATT_y

Cuts at:   1493    2129    2584    2918    3610    3836    3988    6097    1493 
   Size:        636     455     334     692     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     692     636     455     334     226     152
            
 
AseI AT'TA_AT

Cuts at:    160    1032    3231    4855     160 
   Size:        872    2199    1624    1606

  Fragments arranged by size: 

                2199    1624    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6301
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3626    1870 
   Size:         73    1683    4545

  Fragments arranged by size: 

                4545    1683      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2316    2349    2316 
   Size:         33    6268
 
BamHI G'GATC_C

Cuts at:   3631    3998    3631 
   Size:        367    5934
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3622    5077     618 
   Size:        325     217     627    1835    1455    1842

  Fragments arranged by size: 

                1842    1835    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2088    2868    3431    3620    1391 
   Size:        697     780     563     189    4072

  Fragments arranged by size: 

                4072     780     697     563     189
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3219     928 
   Size:        971    1320    4010

  Fragments arranged by size: 

                4010    1320     971
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4492    4526    1903 
   Size:         34    2555      34    3678

  Fragments arranged by size: 

                3678    2555      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2344    2469    4193    5403    2344 
   Size:        125    1724    1210    3242

  Fragments arranged by size: 

                3242    1724    1210     125
 
BclI T'GATC_A

Cuts at:   1548    2637    1548 
   Size:       1089    5212
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4912     136 
   Size:        107     122      71     631     605    3240    1525

  Fragments arranged by size: 

                3240    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6296    6296 
   Size:       6301
 
BlpI GC'TnA_GC

Cuts at:   3718    3718 
   Size:       6301
 
Bme1580I G_kGCm'C

Cuts at:   1790    3431    4362    5518    1790 
   Size:       1641     931    1156    2573

  Fragments arranged by size: 

                2573    1641    1156     931
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6301
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3463    4032    4990 
   Size:        416      53      83     399      24    2046     569     958

Cuts at:   4990     442 
   Size:       1753

  Fragments arranged by size: 

                2046    1753     958     569     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    2163    1056 
   Size:       1107    5194
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2102    3140    4961    1239 
   Size:        863    1038    1821    2579

  Fragments arranged by size: 

                2579    1821    1038     863
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2717    3107    4426    5291    5589    2717 
   Size:        390    1319     865     298    3429

  Fragments arranged by size: 

                3429    1319     865     390     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2921    3269    4964     882 
   Size:        617    1422     348    1695    2219

  Fragments arranged by size: 

                2219    1695    1422     617     348
 
BsaAI yAC'GTr

Cuts at:    493    1204    4092     493 
   Size:        711    2888    2702

  Fragments arranged by size: 

                2888    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   6295    6295 
   Size:       6301
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4108    4490    5125     275 
   Size:         53      83     186     936    2575     382     635    1451

  Fragments arranged by size: 

                2575    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2444    3477    4734    5132    5406    5553    5964    2444 
   Size:       1033    1257     398     274     147     411    2781

  Fragments arranged by size: 

                2781    1257    1033     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2022    2052    1178 
   Size:         30     165      30     619      30    5427

  Fragments arranged by size: 

                5427     619     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2162    3069    3307    1186 
   Size:        976     907     238    4180

  Fragments arranged by size: 

                4180     976     907     238
 
BseYI C'CCAG_C

Cuts at:   2869    3165    5504    6000    2869 
   Size:        296    2339     496    3170

  Fragments arranged by size: 

                3170    2339     496     296
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5728
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3482    3751    4512    5540    1540 
   Size:        275     186    1481     269     761    1028    2301

  Fragments arranged by size: 

                2301    1481    1028     761     275     269     186
 
BsiHKAI G_wGCw'C

Cuts at:   2868    4362    4447    5518    2868 
   Size:       1494      85    1071    3651

  Fragments arranged by size: 

                3651    1494    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2791    2877    3812    3905    2791 
   Size:         86     935      93    5187

  Fragments arranged by size: 

                5187     935      93      86
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2088    2868    3431    3620    4362    4447    5518 
   Size:        399     298     780     563     189     742      85    1071

Cuts at:   5518    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     780     742     563     399     298     189
                  85
 
BspEI T'CCGG_A

Cuts at:   2444    5964    2444 
   Size:       3520    2781
 
BspHI T'CATG_A

Cuts at:   2634    4190    5920    2634 
   Size:       1556    1730    3015

  Fragments arranged by size: 

                3015    1730    1556
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2099    3656     844 
   Size:       1255    1557    3489

  Fragments arranged by size: 

                3489    1557    1255
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4188    1394 
   Size:        429    2365    3507

  Fragments arranged by size: 

                3507    2365     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2113    2277    4796    4970      65 
   Size:       1183     865     164    2519     174    1396

  Fragments arranged by size: 

                2519    1396    1183     865     174     164
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3477    4945    5132    1700 
   Size:        110    1667    1468     187    2869

  Fragments arranged by size: 

                2869    1667    1468     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2769      96 
   Size:       2673    3628
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6301
 
BssSI C'ACGA_G

Cuts at:   3501    4361    5373    3501 
   Size:        860    1012    4429

  Fragments arranged by size: 

                4429    1012     860
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6301
 
BstBI TT'CG_AA

Cuts at:   5105    5105 
   Size:       6301
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6301
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3128    3438    3128 
   Size:        310    5991
 
BstYI r'GATC_y

Cuts at:   1972    2262    3364    3631    3998    4383    4400    5852    5938 
   Size:        290    1102     267     367     385      17    1452      86

Cuts at:   5938    5950    6296    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452    1102     385     367     346     290     267
                  86      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2837    2837 
   Size:       6301
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    3347    3378     513 
   Size:        552     939    1343      31    3436

  Fragments arranged by size: 

                3436    1343     939     552      31
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5562

  Fragments arranged by size: 

                5562     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3111    3443    3817    4611    4631    3111 
   Size:        332     374     794      20    4781

  Fragments arranged by size: 

                4781     794     374     332      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6301
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2077    2112     529 
   Size:         35    1513      35    4718

  Fragments arranged by size: 

                4718    1513      35      35
 
DraI TTT'AAA

Cuts at:   2503    2826    3064    3605    2503 
   Size:        323     238     541    5199

  Fragments arranged by size: 

                5199     541     323     238
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5308     809 
   Size:        507    3992    1802

  Fragments arranged by size: 

                3992    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2019    2371    2934 
   Size:         54    1425      50     275      18     189     352     563

Cuts at:   2934    3375    3440    3748    4637    6266       8 
   Size:        441      65     308     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     563     441     352     308     275
                 189      65      54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3748    1537 
   Size:        275    1936    4090

  Fragments arranged by size: 

                4090    1936     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2067    2096    2194    4231    1353 
   Size:        714      29      98    2037    3423

  Fragments arranged by size: 

                3423    2037     714      98      29
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4888    5262    5408     387 
   Size:       1064     138    3299     374     146    1280

  Fragments arranged by size: 

                3299    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2866    2866 
   Size:       6301
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2102    2327    3140    3503    3569    4364    4961 
   Size:        393     470     225     813     363      66     795     597

Cuts at:   4961    5748    6016    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524     813     795     787     597     470     393     363
                 268     225      66
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2056    2056 
   Size:       6301
 
EcoO109I rG'GnC_Cy

Cuts at:   2119    2119 
   Size:       6301
 
EcoRI G'AATT_C

Cuts at:   2584    2918    3610    3988    2584 
   Size:        334     692     378    4897

  Fragments arranged by size: 

                4897     692     378     334
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3547    3547 
   Size:       6301
 
FspI TGC'GCA

Cuts at:   4807    4807 
   Size:       6301
 
HaeII r_GCGC'y

Cuts at:   1678    5448    1678 
   Size:       3770    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3143    3175    3299    3331    4942 
   Size:         32     135      32    1571      32     124      32    1611

Cuts at:   4942    4974    5016    5048    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1611    1571     135     124      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3096    3151    3597    3645    3891     669 
   Size:        988     281    1158      55     446      48     246    3079

  Fragments arranged by size: 

                3079    1158     988     446     281     246      55      48
            
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       6301
 
HpaI GTT'AAC

Cuts at:   3891    3891 
   Size:       6301
 
KpnI G_GTAC'C

Cuts at:   3626    3626 
   Size:       6301
 
MfeI C'AATT_G

Cuts at:   3900    3900 
   Size:       6301
 
MluI A'CGCG_T

Cuts at:   6177    6177 
   Size:       6301
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2208    5415    5599    1305 
   Size:        903    3207     184    2007

  Fragments arranged by size: 

                3207    2007     903     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2021    2373    3377    3442      10 
   Size:         54    1425     343     189     352    1004      65    2869

  Fragments arranged by size: 

                2869    1425    1004     352     343     189      65      54
            
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3104    4259    4618    4777     518 
   Size:        648    1938    1155     359     159    2042

  Fragments arranged by size: 

                2042    1938    1155     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3165    3717    4394    5542    5787    1606 
   Size:       1559     552     677    1148     245    2120

  Fragments arranged by size: 

                2120    1559    1148     677     552     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    3347    3378     513 
   Size:        552     939    1343      31    3436

  Fragments arranged by size: 

                3436    1343     939     552      31
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6301
 
NheI G'CTAG_C

Cuts at:   1052    2159    1052 
   Size:       1107    5194
 
NotI GC'GGCC_GC

Cuts at:   3748    3748 
   Size:       6301
 
NspI r_CATG'y

Cuts at:   1132    1229    2704    3659    5204    1132 
   Size:         97    1475     955    1545    2229

  Fragments arranged by size: 

                2229    1545    1475     955      97
 
PasI CC'CwG_GG

Cuts at:   1348    3431    1348 
   Size:       2083    4218
 
PciI A'CATG_T

Cuts at:   5200    5200 
   Size:       6301
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6301
 
PfoI T'CCnGG_A

Cuts at:   2265    2485    2265 
   Size:        220    6081
 
PmeI GTTT'AAAC

Cuts at:   3605    3605 
   Size:       6301
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4370    4402    5883    5915    4370 
   Size:         32    1481      32    4756

  Fragments arranged by size: 

                4756    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6301
 
PsiI TTA'TAA

Cuts at:   3871    3871 
   Size:       6301
 
PspOMI G'GGCC_C

Cuts at:   3427    3427 
   Size:       6301
 
PstI C_TGCA'G

Cuts at:    830    3653     830 
   Size:       2823    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6301
 
SacI G_AGCT'C

Cuts at:   2868    2868 
   Size:       6301
 
SalI G'TCGA_C

Cuts at:   1936    3643    1936 
   Size:       1707    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2096    2096 
   Size:       6301
 
SbfI CC_TGCA'GG

Cuts at:   3653    3653 
   Size:       6301
 
ScaI AGT'ACT

Cuts at:   4549    6261    4549 
   Size:       1712    4589
 
SexAI A'CCwGG_T

Cuts at:   2539    2539 
   Size:       6301
 
SfcI C'TryA_G

Cuts at:    826    1045    3649    4784    5465    5656     826 
   Size:        219    2604    1135     681     191    1471

  Fragments arranged by size: 

                2604    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6301
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6301
 
SmaI CCC'GGG

Cuts at:   1872    3628    1872 
   Size:       1756    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2732    3086    4405    5306    5568     820 
   Size:        197     926     789     354    1319     901     262    1553

  Fragments arranged by size: 

                1553    1319     926     901     789     354     262     197
            
 
SnaBI TAC'GTA

Cuts at:    493    4092     493 
   Size:       3599    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6301
 
SphI G_CATG'C

Cuts at:   1132    3659    1132 
   Size:       2527    3774
 
SspI AAT'ATT

Cuts at:      5      52    4225       5 
   Size:         47    4173    2081

  Fragments arranged by size: 

                4173    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6301
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2693    3127    3347    3378    3740     513 
   Size:        552     939     689     434     220      31     362    3074

  Fragments arranged by size: 

                3074     939     689     552     434     362     220      31
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2650    4328    4345    4498    4683    5128     325 
   Size:       1255    1070    1678      17     153     185     445    1498

  Fragments arranged by size: 

                1678    1498    1255    1070     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2310    2769    3194 
   Size:        274      80      33      51    1443     333     459     425

Cuts at:   3194    4547    6259      96 
   Size:       1353    1712     138

  Fragments arranged by size: 

                1712    1443    1353     459     425     333     274     138
                  80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2022    2054    2439    2471    6261    6293    2022 
   Size:         32     385      32    3790      32    2030

  Fragments arranged by size: 

                3790    2030     385      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6301
 
XbaI T'CTAG_A

Cuts at:   3637    3637 
   Size:       6301
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3376    3376 
   Size:       6301
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6301
 
XmaI C'CCGG_G

Cuts at:   1870    3626    1870 
   Size:       1756    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2918    4430    2918 
   Size:       1512    4789
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4109     276 
   Size:         53      83     186     936    2575    2468

  Fragments arranged by size: 

                2575    2468     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbsI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PshAI     PsiI   PspOMI     PstI     PvuI     SacI     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     AscI    AvrII     BbeI    BbvCI    BfrBI     BplI
   Bpu10I    BsiWI    BsmBI  BstZ17I   DraIII    EcoRV     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PmlI
    PpuMI    PspXI     PsrI    PvuII    RsrII    SacII    SanDI     SfiI
     SfoI     SrfI     SwaI
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