(Linear) (Six-base) MAPSORT of: KIEE4510.seq Check: 2314 from: 1 to: 1596 KIEE4510.orf With 182 enzymes: SgfI * July 3, 2012 19:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 94 1596 Size: 94 1502 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 322 1498 1564 1596 Size: 322 1176 66 32 Fragments arranged by size: 1176 322 66 32 AgeI A'CCGG_T Cuts at: 0 1472 1596 Size: 1472 124 AhdI GACnn_n'nnGTC Cuts at: 0 1152 1596 Size: 1152 444 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 797 1596 Size: 797 799 AlwNI CAG_nnn'CTG Cuts at: 0 59 1596 Size: 59 1537 ApaI G_GGCC'C Cuts at: 0 1426 1596 Size: 1426 170 ApoI r'AATT_y Cuts at: 0 124 579 913 1596 Size: 124 455 334 683 Fragments arranged by size: 683 455 334 124 AseI AT'TA_AT Cuts at: 0 1226 1596 Size: 1226 370 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 311 344 1596 Size: 311 33 1252 Fragments arranged by size: 1252 311 33 BanII G_rGCy'C Cuts at: 0 83 863 1426 1596 Size: 83 780 563 170 Fragments arranged by size: 780 563 170 83 BbsI GAAGACnn'nnnn_ Cuts at: 0 1214 1596 Size: 1214 382 BciVI GTATCCnnnnn_n' Cuts at: 0 339 464 1596 Size: 339 125 1132 Fragments arranged by size: 1132 339 125 BclI T'GATC_A Cuts at: 0 632 1596 Size: 632 964 Bme1580I G_kGCm'C Cuts at: 0 1426 1596 Size: 1426 170 BmrI ACTGGGnnnn_n' Cuts at: 0 1458 1596 Size: 1458 138 BmtI G_CTAG'C Cuts at: 0 158 1596 Size: 158 1438 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 1135 1596 Size: 97 1038 461 Fragments arranged by size: 1038 461 97 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 712 1102 1596 Size: 712 390 494 Fragments arranged by size: 712 494 390 BsaI GGTCTCn'nnnn_ Cuts at: 0 916 1264 1596 Size: 916 348 332 Fragments arranged by size: 916 348 332 BsaWI w'CCGG_w Cuts at: 0 439 1472 1596 Size: 439 1033 124 Fragments arranged by size: 1033 439 124 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 17 47 1596 Size: 17 30 1549 Fragments arranged by size: 1549 30 17 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 157 1064 1302 1596 Size: 157 907 238 294 Fragments arranged by size: 907 294 238 157 BseYI C'CCAG_C Cuts at: 0 864 1160 1596 Size: 864 296 436 Fragments arranged by size: 864 436 296 BsiEI CG_ry'CG Cuts at: 0 1477 1596 Size: 1477 119 BsiHKAI G_wGCw'C Cuts at: 0 863 1596 Size: 863 733 BsmI GAATG_Cn' Cuts at: 0 786 872 1596 Size: 786 86 724 Fragments arranged by size: 786 724 86 Bsp1286I G_dGCh'C Cuts at: 0 83 863 1426 1596 Size: 83 780 563 170 Fragments arranged by size: 780 563 170 83 BspEI T'CCGG_A Cuts at: 0 439 1596 Size: 439 1157 BspHI T'CATG_A Cuts at: 0 629 1596 Size: 629 967 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 94 1596 Size: 94 1502 BsrDI GCAATG_nn' Cuts at: 0 108 272 1596 Size: 108 164 1324 Fragments arranged by size: 1324 164 108 BsrFI r'CCGG_y Cuts at: 0 1472 1596 Size: 1472 124 BsrGI T'GTAC_A Cuts at: 0 764 1596 Size: 764 832 BssSI C'ACGA_G Cuts at: 0 1496 1596 Size: 1496 100 BstXI CCAn_nnnn'nTGG Cuts at: 0 1123 1433 1596 Size: 1123 310 163 Fragments arranged by size: 1123 310 163 BstYI r'GATC_y Cuts at: 0 257 1359 1596 Size: 257 1102 237 Fragments arranged by size: 1102 257 237 Bsu36I CC'TnA_GG Cuts at: 0 832 1596 Size: 832 764 BtgI C'CryG_G Cuts at: 0 1342 1373 1596 Size: 1342 31 223 Fragments arranged by size: 1342 223 31 BtsI GCAGTG_nn' Cuts at: 0 1106 1438 1596 Size: 1106 332 158 Fragments arranged by size: 1106 332 158 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 72 107 1596 Size: 72 35 1489 Fragments arranged by size: 1489 72 35 DraI TTT'AAA Cuts at: 0 498 821 1059 1596 Size: 498 323 238 537 Fragments arranged by size: 537 498 323 238 EaeI y'GGCC_r Cuts at: 0 14 366 929 1370 1435 1596 Size: 14 352 563 441 65 161 Fragments arranged by size: 563 441 352 161 65 14 EarI CTCTTCn'nnn_ Cuts at: 0 62 91 189 1596 Size: 62 29 98 1407 Fragments arranged by size: 1407 98 62 29 EcoICRI GAG'CTC Cuts at: 0 861 1596 Size: 861 735 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 322 1135 1498 1564 1596 Size: 97 225 813 363 66 32 Fragments arranged by size: 813 363 225 97 66 32 EcoNI CCTnn'n_nnAGG Cuts at: 0 51 1596 Size: 51 1545 EcoO109I rG'GnC_Cy Cuts at: 0 114 1596 Size: 114 1482 EcoRI G'AATT_C Cuts at: 0 579 913 1596 Size: 579 334 683 Fragments arranged by size: 683 579 334 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1542 1596 Size: 1542 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1138 1170 1294 1326 1596 Size: 1138 32 124 32 270 Fragments arranged by size: 1138 270 124 32 32 HincII GTy'rAC Cuts at: 0 1091 1146 1592 1596 Size: 1091 55 446 4 Fragments arranged by size: 1091 446 55 4 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 203 1596 Size: 203 1393 MscI TGG'CCA Cuts at: 0 16 368 1372 1437 1596 Size: 16 352 1004 65 159 Fragments arranged by size: 1004 352 159 65 16 MslI CAynn'nnrTG Cuts at: 0 1099 1596 Size: 1099 497 MspA1I CmG'CkG Cuts at: 0 1160 1596 Size: 1160 436 NcoI C'CATG_G Cuts at: 0 1342 1373 1596 Size: 1342 31 223 Fragments arranged by size: 1342 223 31 NheI G'CTAG_C Cuts at: 0 154 1596 Size: 154 1442 NspI r_CATG'y Cuts at: 0 699 1596 Size: 699 897 PasI CC'CwG_GG Cuts at: 0 1426 1596 Size: 1426 170 PfoI T'CCnGG_A Cuts at: 0 260 480 1596 Size: 260 220 1116 Fragments arranged by size: 1116 260 220 PspOMI G'GGCC_C Cuts at: 0 1422 1596 Size: 1422 174 SacI G_AGCT'C Cuts at: 0 863 1596 Size: 863 733 SapI GCTCTTCn'nnn_ Cuts at: 0 91 1596 Size: 91 1505 SexAI A'CCwGG_T Cuts at: 0 534 1596 Size: 534 1062 SmlI C'TyrA_G Cuts at: 0 727 1081 1596 Size: 727 354 515 Fragments arranged by size: 727 515 354 StyI C'CwwG_G Cuts at: 0 688 1122 1342 1373 1596 Size: 688 434 220 31 223 Fragments arranged by size: 688 434 223 220 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 645 1596 Size: 645 951 TatI w'GTAC_w Cuts at: 0 305 764 1189 1596 Size: 305 459 425 407 Fragments arranged by size: 459 425 407 305 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 17 49 434 466 1596 Size: 17 32 385 32 1130 Fragments arranged by size: 1130 385 32 32 17 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1371 1596 Size: 1371 225 XmnI GAAnn'nnTTC Cuts at: 0 913 1596 Size: 913 683 Enzymes that do cut: AarI AcuI AgeI AhdI AlfI AlwNI ApaI ApoI AseI BaeI BanII BbsI BciVI BclI Bme1580I BmrI BmtI BpmI BpuEI BsaI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BssSI BstXI BstYI Bsu36I BtgI BtsI CspCI DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII MmeI MscI MslI MspA1I NcoI NheI NspI PasI PfoI PspOMI SacI SapI SexAI SmlI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AleI AloI ApaLI AscI AsiSI AvaI AvrII BamHI BanI BbeI BbvCI BcgI BfrBI BglI BglII BlpI BmgBI BplI Bpu10I BsaAI BsaBI BsaHI BsgI BsiWI BsmBI BsrBI BssHII BstAPI BstBI BstEII BstZ17I BtgZI ClaI DraIII DrdI EagI EciI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI ZraI