################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BclI BcnI BfaI BfrI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV FauI FbaI FseI FspI HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MroI MunI MvnI NaeI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SfaNI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AcsI 1352 GAATTT [GA]^AATT[CT] AcyI 918 GGCGCC G[GA]^CG[CT]C AflIII 497 ACATGT A^C[GA][CT]GT AlwI 51 GATCC GGATC(4/5) AlwNI 27 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 1352 GAATTT [GA]^AATT[CT] AspHI 400 GAGCAC G[AT]GC[AT]^C AvaI 397 CCCGAG C^[CT]CG[GA]G BalI 297 TGGCCA TGG^CCA BanI 918 GGCGCC G^G[CT][GA]CC BbeI 918 GGCGCC GGCGC^C BciVI 484 GTATCC GTATCC(6/5) BglI 355 GCCGAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 161 AGATCT A^GATCT BsaHI 918 GGCGCC G[GA]^CG[CT]C BseRI 819 CTCCTC GAGGAG(10/8) BsiHKAI 400 GAGCAC G[AT]GC[AT]^C BsmFI 1022 GGGAC GGGAC(10/14) BsmI 1307 GCATTC GAATGC(1/-1) BsoBI 397 CCCGAG C^[CT]CG[GA]G Bsp1407I 1407 TGTACA T^GTACA BspHI 1259 TCATGA T^CATGA BspLU11I 497 ACATGT A^CATGT BsrBI 417 GAGCGG CCGCTC(-3/-3) BsrDI 764 GCAATG GCAATG(2/0) BsrFI 1069 GCCGGT [GA]^CCGG[CT] BsrGI 1407 TGTACA T^GTACA BstAPI 1339 GCAGTAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 345 GGTCACC G^GT[ACGT]ACC BstPI 345 GGTCACC G^GT[ACGT]ACC BstYI 161 AGATCT [GA]^GATC[CT] CfoI 919 GCGC GCG^C Cfr10I 1069 GCCGGT [GA]^CCGG[CT] DraII 1022 GGGACCT [GA]G^G[ACGT]CC[CT] DsaI 542 CCGTGG C^C[GA][CT]GG EcoO109I 1022 GGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 345 GGTCACC G^GT[ACGT]ACC EcoT22I 1087 ATGCAT ATGCA^T HaeII 918 GGCGCC [GA]GCGC^[CT] HapII 1070 CCGG C^CGG HgaI 434 GCGTC GACGC(5/10) HhaI 919 GCGC GCG^C Hin1I 918 GGCGCC G[GA]^CG[CT]C HinP1I 919 GCGC G^CGC HpaII 1070 CCGG C^CGG KasI 918 GGCGCC G^GCGCC MflI 161 AGATCT [GA]^GATC[CT] MluNI 297 TGGCCA TGG^CCA MscI 297 TGGCCA TGG^CCA MspA1I 742 CAGCTG C[AC]G^C[GT]G MspI 1070 CCGG C^CGG NarI 918 GGCGCC GG^CGCC NsiI 1087 ATGCAT ATGCA^T PflMI 1100 CCAATATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1022 GGGACCT [GA]G^G[AT]CC[CT] PstI 426 CTGCAG CTGCA^G PvuII 742 CAGCTG CAG^CTG RcaI 1259 TCATGA T^CATGA SapI 685 GAAGAGC GCTCTTC(1/4) SfcI 426 CTGCAG C^T[GA][CT]AG SfoI 918 GGCGCC GGC^GCC SspBI 1407 TGTACA T^GTACA Van91I 1100 CCAATATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 161 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 504 GTAC GT^AC 1408 GTAC AvaII 121 GGACC G^G[AT]CC 1023 GGACC BanII 1288 GGGCTC G[GA]GC[CT]^C 1413 GAGCCC BbsI 309 GAAGAC GAAGAC(2/6) 1051 GTCTTC BpuAI 309 GAAGAC GAAGAC(2/6) 1051 GTCTTC BsaI 84 GAGACC GGTCTC(1/5) 842 GGTCTC BsgI 143 GTGCAG GTGCAG(16/14) 787 GTGCAG BsiYI 971 CCTTTGGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1100 CCAATATCTGG BslI 971 CCTTTGGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1100 CCAATATCTGG BspMI 318 GCAGGT ACCTGC(4/8) 868 GCAGGT EaeI 297 TGGCCA [CT]^GGCC[GA] 443 TGGCCG EarI 685 GAAGAG CTCTTC(1/4) 1456 GAAGAG HincII 1015 GTCAAC GT[CT]^[GA]AC 1476 GTCAAC HindII 1015 GTCAAC GT[CT]^[GA]AC 1476 GTCAAC Ksp632I 685 GAAGAG CTCTTC(1/4) 1456 GAAGAG MseI 875 TTAA T^TAA 1187 TTAA MslI 661 CACGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1260 CATGACTGTG RsaI 504 GTAC GT^AC 1408 GTAC SexAI 188 ACCTGGT A^CC[AT]GGT 878 ACCAGGT Tru9I 875 TTAA T^TAA 1187 TTAA Tsp509I 1344 AATT ^AATT 1353 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AluI BbvI BmyI BsaJI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeII MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV NspI Sau3AI Sau96I ScrFI StyI TfiI TseI Tsp45I TspRI