Restriction map for
pFN21AE0972
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbeI BcgI BciVI
BclI BfrI BglII BlnI BsaI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspEI BspHI BspLU11I BsrFI BsrGI
BssSI Bst1107I BstBI BstXI BstZ17I
Cfr10I CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HincII HindII HpaI KasI KpnI
KspI MfeI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 886 GACGTC GACGT^C
AccII 791 CGCG CG^CG
AlwNI 565 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1231 CCTGAGG CC^T[ACGT]AGG
ApaI 820 GGGCCC GGGCC^C
BalI 341 TGGCCA TGG^CCA
BbsI 448 GAAGAC GAAGAC(2/6)
BglI 1066 GCCCCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1027 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1027 GCTCAGC GC^T[ACGT]AGC
BpuAI 448 GAAGAC GAAGAC(2/6)
BsaBI 597 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 367 GTGCAG GTGCAG(16/14)
BsmAI 1297 GAGAC GTCTC(1/5)
BsmBI 1297 GAGACG CGTCTC(1/5)
BsmI 1057 GCATTC GAATGC(1/-1)
BspDI 67 ATCGAT AT^CGAT
BsrDI 463 GCAATG GCAATG(2/0)
BssHII 790 GCGCGC G^CGCGC
BstAPI 564 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1338 GGTGACC G^GT[ACGT]ACC
BstPI 1338 GGTGACC G^GT[ACGT]ACC
BstUI 791 CGCG CG^CG
Bsu36I 1231 CCTGAGG CC^T[ACGT]AGG
CelII 1027 GCTCAGC GC^T[ACGT]AGC
ClaI 67 ATCGAT AT^CGAT
DraIII 334 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 991 GACTTTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1522 GAAGAG CTCTTC(1/4)
Eco81I 1231 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1338 GGTGACC G^GT[ACGT]ACC
HindIII 214 AAGCTT A^AGCTT
Ksp632I 1522 GAAGAG CTCTTC(1/4)
MamI 597 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 341 TGGCCA TGG^CCA
MscI 341 TGGCCA TGG^CCA
MseI 1415 TTAA T^TAA
MvnI 791 CGCG CG^CG
PaeR7I 901 CTCGAG C^TCGAG
PflMI 333 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1255 GAGCTC GAGCT^C
SapI 1522 GAAGAGC GCTCTTC(1/4)
SfaNI 352 GATGC GCATC(5/9)
SfiI 1065 GGCCCCCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 302 CCCGGG CCC^GGG
StuI 1109 AGGCCT AGG^CCT
Tru9I 1415 TTAA T^TAA
Tsp509I 1305 AATT ^AATT
Van91I 333 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 901 CTCGAG C^TCGAG
XmaCI 302 CCCGGG C^CCGGG
XmaI 302 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AspHI 1255 GAGCTC G[AT]GC[AT]^C
1378 GAGCAC
BamHI 30 GGATCC G^GATCC
775 GGATCC
BanI 409 GGTGCC G^G[CT][GA]CC
1311 GGTGCC
BbvCI 664 GCTGAGG CCTCAGC(-5/-2)
1473 GCTGAGG
BfaI 113 CTAG C^TAG
197 CTAG
BsiHKAI 1255 GAGCTC G[AT]GC[AT]^C
1378 GAGCAC
BspMI 878 ACCTGC ACCTGC(4/8)
953 ACCTGC
BsrBI 229 GAGCGG CCGCTC(-3/-3)
580 GAGCGG
BstYI 30 GGATCC [GA]^GATC[CT]
775 GGATCC
EcoNI 308 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
810 CCTGAGAAAGG
EcoT14I 1112 CCTTGG C^C[AT][AT]GG
1333 CCTTGG
MaeI 113 CTAG C^TAG
197 CTAG
MflI 30 GGATCC [GA]^GATC[CT]
775 GGATCC
PleI 75 GACTC GAGTC(4/5)
328 GAGTC
ScaI 169 AGTACT AGT^ACT
905 AGTACT
SmlI 901 CTCGAG C^T[CT][GA]AG
1258 CTCAAG
StyI 1112 CCTTGG C^C[AT][AT]GG
1333 CCTTGG
XhoII 30 GGATCC [GA]^GATC[CT]
775 GGATCC
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AvaI AvaII BanII BbrPI BbvI
BcnI BmyI BpmI BsaAI BsaHI
BsaJI BseRI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfcI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XcmI