################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 1615 GTCTAC GT^[AC][GT]AC AcyI 984 GGCGTC G[GA]^CG[CT]C AflIII 686 ACATGT A^C[GA][CT]GT BanI 1555 GGCACC G^G[CT][GA]CC BbsI 1293 GAAGAC GAAGAC(2/6) BlpI 827 GCTCAGC GC^T[ACGT]AGC Bpu1102I 827 GCTCAGC GC^T[ACGT]AGC BpuAI 1293 GAAGAC GAAGAC(2/6) BsaBI 142 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 984 GGCGTC G[GA]^CG[CT]C BseRI 664 CTCCTC GAGGAG(10/8) BspHI 1155 TCATGA T^CATGA BspLU11I 686 ACATGT A^CATGT BsrDI 1238 CATTGC GCAATG(2/0) BstYI 511 AGATCC [GA]^GATC[CT] CelII 827 GCTCAGC GC^T[ACGT]AGC CfoI 1048 GCGC GCG^C DraIII 1422 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 259 GACTGCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 360 TGGCCG [CT]^GGCC[GA] HaeII 1047 GGCGCT [GA]GCGC^[CT] HgaI 985 GCGTC GACGC(5/10) HhaI 1048 GCGC GCG^C Hin1I 984 GGCGTC G[GA]^CG[CT]C HinP1I 1048 GCGC G^CGC HincII 628 GTCAAC GT[CT]^[GA]AC HindII 628 GTCAAC GT[CT]^[GA]AC HindIII 880 AAGCTT A^AGCTT MamI 142 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 511 AGATCC [GA]^GATC[CT] PstI 735 CTGCAG CTGCA^G RcaI 1155 TCATGA T^CATGA SfcI 735 CTGCAG C^T[GA][CT]AG SmlI 1243 CTCAAG C^T[CT][GA]AG SphI 636 GCATGC GCATG^C XbaI 1275 TCTAGA T^CTAGA XhoII 511 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 201 GTAC GT^AC 1191 GTAC AlwNI 1132 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 1387 CAGCTCCTG Asp700I 1147 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1179 GAAGATATTC BfaI 1123 CTAG C^TAG 1276 CTAG BsaAI 339 TACGTG [CT]AC^GT[GA] 1288 TACGTG BsgI 1086 CTGCAC GTGCAG(16/14) 1478 GTGCAG BsmAI 374 GTCTC GTCTC(1/5) 817 GTCTC BspMI 490 ACCTGC ACCTGC(4/8) 1510 ACCTGC EarI 154 GAAGAG CTCTTC(1/4) 1602 GAAGAG EcoNI 421 CCTTACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 666 CCTCTTGCAGG Ksp632I 154 GAAGAG CTCTTC(1/4) 1602 GAAGAG MaeI 1123 CTAG C^TAG 1276 CTAG PflMI 1255 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1386 CCAGCTCCTGG PvuII 291 CAGCTG CAG^CTG 435 CAGCTG RsaI 201 GTAC GT^AC 1191 GTAC Tsp45I 1265 GTCAC ^GT[GC]AC 1520 GTGAC Van91I 1255 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1386 CCAGCTCCTGG XcmI 1032 CCACTTCTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1515 CCAGGGTGACTCTGG XmnI 1147 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1179 GAAGATATTC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AspHI AvaII BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I TseI Tsp509I TspRI