(Circular) (Six-base) MAPSORT of: pFN21AE1552.seq Check: 8166 from: 1 to: 6385 pFN21AE1552 6385 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3524 3524 Size: 6385 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4195 278 Size: 53 83 186 936 2659 2468 Fragments arranged by size: 2659 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3621 3728 1937 Size: 1684 107 4594 Fragments arranged by size: 4594 1684 107 Acc65I G'GTAC_C Cuts at: 3706 3706 Size: 6385 AclI AA'CG_TT Cuts at: 1563 4512 4885 1563 Size: 2949 373 3063 Fragments arranged by size: 3063 2949 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3164 3479 4448 5832 6100 1632 Size: 1532 315 969 1384 268 1917 Fragments arranged by size: 1917 1532 1384 969 315 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6188 AflIII A'CryG_T Cuts at: 1203 2691 5284 6261 1203 Size: 1488 2593 977 1327 Fragments arranged by size: 2593 1488 1327 977 AgeI A'CCGG_T Cuts at: 5216 5216 Size: 6385 AhdI GACnn_n'nnGTC Cuts at: 5114 5114 Size: 6385 AleI CACnn'nnGTG Cuts at: 1166 3042 1166 Size: 1876 4509 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6385 AlwNI CAG_nnn'CTG Cuts at: 3142 3397 4128 5700 3142 Size: 255 731 1572 3827 Fragments arranged by size: 3827 1572 731 255 ApaLI G'TGCA_C Cuts at: 4442 5598 4442 Size: 1156 5229 ApoI r'AATT_y Cuts at: 1493 2558 3155 3638 3694 3920 4072 6181 1493 Size: 1065 597 483 56 226 152 2109 1697 Fragments arranged by size: 2109 1697 1065 597 483 226 152 56 AseI AT'TA_AT Cuts at: 160 1032 4939 160 Size: 872 3907 1606 Fragments arranged by size: 3907 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6385 AvaI C'yCGr_G Cuts at: 1870 1943 3710 1870 Size: 73 1767 4545 Fragments arranged by size: 4545 1767 73 BamHI G'GATC_C Cuts at: 3715 4082 3715 Size: 367 6018 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3560 3706 5161 618 Size: 325 217 627 1773 146 1455 1842 Fragments arranged by size: 1842 1773 1455 627 325 217 146 BanII G_rGCy'C Cuts at: 1391 3704 1391 Size: 2313 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3305 928 Size: 971 1406 4008 Fragments arranged by size: 4008 1406 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4576 4610 1903 Size: 34 2639 34 3678 Fragments arranged by size: 3678 2639 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4277 5487 4277 Size: 1210 5175 BclI T'GATC_A Cuts at: 1548 1548 Size: 6385 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4996 136 Size: 107 122 71 631 605 3324 1525 Fragments arranged by size: 3324 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6380 6380 Size: 6385 BlpI GC'TnA_GC Cuts at: 2833 3802 2833 Size: 969 5416 Bme1580I G_kGCm'C Cuts at: 1790 4446 5602 1790 Size: 2656 1156 2573 Fragments arranged by size: 2656 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6385 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4116 5074 442 Size: 416 53 83 399 24 2699 958 1753 Fragments arranged by size: 2699 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6385 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2981 3552 3617 5045 1239 Size: 1742 571 65 1428 2579 Fragments arranged by size: 2579 1742 1428 571 65 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3233 4510 5375 5673 3233 Size: 1277 865 298 3945 Fragments arranged by size: 3945 1277 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5048 882 Size: 617 3549 2219 Fragments arranged by size: 3549 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2346 3295 4176 493 Size: 711 1142 949 881 2702 Fragments arranged by size: 2702 1142 949 881 711 BsaBI GATnn'nnATC Cuts at: 2151 6379 2151 Size: 4228 2157 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2990 4192 4574 5209 Size: 53 83 186 936 1457 1202 382 635 Cuts at: 5209 275 Size: 1451 Fragments arranged by size: 1457 1451 1202 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4818 5216 5490 5637 6048 4818 Size: 398 274 147 411 5155 Fragments arranged by size: 5155 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2645 2675 1178 Size: 30 165 30 1242 30 4888 Fragments arranged by size: 4888 1242 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2660 1186 Size: 1474 4911 BseYI C'CCAG_C Cuts at: 2438 3069 3357 3588 5588 6084 2438 Size: 631 288 231 2000 496 2739 Fragments arranged by size: 2739 2000 631 496 288 231 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3076 3504 1163 Size: 573 1340 428 4044 Fragments arranged by size: 4044 1340 573 428 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3835 4596 5624 1540 Size: 275 186 1834 761 1028 2301 Fragments arranged by size: 2301 1834 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2285 2966 3078 3280 4446 4531 5602 2285 Size: 681 112 202 1166 85 1071 3068 Fragments arranged by size: 3068 1166 1071 681 202 112 85 BsmI GAATG_Cn' Cuts at: 3896 3989 3896 Size: 93 6292 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2285 2966 3078 3280 3704 4446 4531 Size: 399 495 681 112 202 424 742 85 Cuts at: 4531 5602 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 681 495 424 399 202 112 85 BspEI T'CCGG_A Cuts at: 6048 6048 Size: 6385 BspHI T'CATG_A Cuts at: 3160 4274 6004 3160 Size: 1114 1730 3541 Fragments arranged by size: 3541 1730 1114 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2504 3524 3740 844 Size: 1660 1020 216 3489 Fragments arranged by size: 3489 1660 1020 216 BsrBI CCG'CTC Cuts at: 1394 1823 4272 1394 Size: 429 2449 3507 Fragments arranged by size: 3507 2449 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3242 4880 5054 65 Size: 1183 1994 1638 174 1396 Fragments arranged by size: 1994 1638 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5029 5216 1700 Size: 110 3219 187 2869 Fragments arranged by size: 3219 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6385 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6385 BssSI C'ACGA_G Cuts at: 4445 5457 4445 Size: 1012 5373 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6385 BstBI TT'CG_AA Cuts at: 5189 5189 Size: 6385 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6385 BstXI CCAn_nnnn'nTGG Cuts at: 2723 3044 3353 2723 Size: 321 309 5755 Fragments arranged by size: 5755 321 309 BstYI r'GATC_y Cuts at: 1972 2516 3715 4082 4467 4484 5936 6022 6034 Size: 544 1199 367 385 17 1452 86 12 Cuts at: 6034 6380 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1199 544 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2275 2716 2981 513 Size: 552 1210 441 265 3917 Fragments arranged by size: 3917 1210 552 441 265 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2610 487 Size: 683 56 1384 4262 Fragments arranged by size: 4262 1384 683 56 BtsI GCAGTG_nn' Cuts at: 3078 3088 3901 4695 4715 3078 Size: 10 813 794 20 4748 Fragments arranged by size: 4748 813 794 20 10 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6385 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2344 2379 529 Size: 35 1780 35 4535 Fragments arranged by size: 4535 1780 35 35 DraI TTT'AAA Cuts at: 3689 3689 Size: 6385 DraIII CAC_nnn'GTG Cuts at: 3432 3432 Size: 6385 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2270 5392 809 Size: 507 954 3122 1802 Fragments arranged by size: 3122 1802 954 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2365 3832 4721 Size: 54 1425 50 275 18 535 1467 889 Cuts at: 4721 6350 8 Size: 1629 43 Fragments arranged by size: 1629 1467 1425 889 535 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3832 1537 Size: 275 2020 4090 Fragments arranged by size: 4090 2020 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2154 3602 4315 1353 Size: 801 1448 713 3423 Fragments arranged by size: 3423 1448 801 713 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4972 5346 5492 387 Size: 1064 138 3383 374 146 1280 Fragments arranged by size: 3383 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2981 3164 3479 3552 3617 4448 5045 Size: 393 1349 183 315 73 65 831 597 Cuts at: 5045 5832 6100 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1349 831 787 597 393 315 268 183 73 65 EcoNI CCTnn'n_nnAGG Cuts at: 2430 2675 2430 Size: 245 6140 EcoO109I rG'GnC_Cy Cuts at: 2435 3139 3321 3384 3536 3592 2435 Size: 704 182 63 152 56 5228 Fragments arranged by size: 5228 704 182 152 63 56 EcoRI G'AATT_C Cuts at: 3694 4072 3694 Size: 378 6007 FspI TGC'GCA Cuts at: 4891 4891 Size: 6385 HaeII r_GCGC'y Cuts at: 1678 3056 5532 1678 Size: 1378 2476 2531 Fragments arranged by size: 2531 2476 1378 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2008 2040 2588 2620 5026 Size: 32 135 32 436 32 548 32 2406 Cuts at: 5026 5058 5100 5132 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2406 548 436 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2635 3729 3975 669 Size: 988 281 697 1094 246 3079 Fragments arranged by size: 3079 1094 988 697 281 246 HindIII A'AGCT_T Cuts at: 748 2885 748 Size: 2137 4248 HpaI GTT'AAC Cuts at: 3975 3975 Size: 6385 KpnI G_GTAC'C Cuts at: 3710 3710 Size: 6385 MfeI C'AATT_G Cuts at: 3984 3984 Size: 6385 MluI A'CGCG_T Cuts at: 6261 6261 Size: 6385 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5499 5683 1305 Size: 4194 184 2007 Fragments arranged by size: 4194 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4563 Fragments arranged by size: 4563 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2696 3023 3042 3207 4343 4702 4861 Size: 648 1530 327 19 165 1136 359 159 Cuts at: 4861 518 Size: 2042 Fragments arranged by size: 2042 1530 1136 648 359 327 165 159 19 MspA1I CmG'CkG Cuts at: 1606 2298 2409 2442 3801 4478 5626 5871 1606 Size: 692 111 33 1359 677 1148 245 2120 Fragments arranged by size: 2120 1359 1148 692 677 245 111 33 NcoI C'CATG_G Cuts at: 513 1065 2275 2716 2981 513 Size: 552 1210 441 265 3917 Fragments arranged by size: 3917 1210 552 441 265 NdeI CA'TA_TG Cuts at: 387 387 Size: 6385 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6385 NotI GC'GGCC_GC Cuts at: 3832 3832 Size: 6385 NspI r_CATG'y Cuts at: 1132 1229 2295 2645 2695 3743 5288 1132 Size: 97 1066 350 50 1048 1545 2229 Fragments arranged by size: 2229 1545 1066 1048 350 97 50 PasI CC'CwG_GG Cuts at: 1348 3031 1348 Size: 1683 4702 PciI A'CATG_T Cuts at: 2691 5284 2691 Size: 2593 3792 PflMI CCAn_nnn'nTGG Cuts at: 1108 3266 3397 1108 Size: 2158 131 4096 Fragments arranged by size: 4096 2158 131 PfoI T'CCnGG_A Cuts at: 3396 3396 Size: 6385 PmeI GTTT'AAAC Cuts at: 3689 3689 Size: 6385 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4454 4486 5967 5999 4454 Size: 32 1481 32 4840 Fragments arranged by size: 4840 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2435 3139 3321 3384 2435 Size: 704 182 63 5436 Fragments arranged by size: 5436 704 182 63 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6385 PsiI TTA'TAA Cuts at: 2151 3955 2151 Size: 1804 4581 PstI C_TGCA'G Cuts at: 830 2744 3737 830 Size: 1914 993 3478 Fragments arranged by size: 3478 1914 993 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6385 PvuII CAG'CTG Cuts at: 2298 2442 2298 Size: 144 6241 SalI G'TCGA_C Cuts at: 1936 3727 1936 Size: 1791 4594 SanDI GG'GwC_CC Cuts at: 3384 3384 Size: 6385 SbfI CC_TGCA'GG Cuts at: 3737 3737 Size: 6385 ScaI AGT'ACT Cuts at: 4633 6345 4633 Size: 1712 4673 SfcI C'TryA_G Cuts at: 826 1045 2740 3733 4868 5549 5740 826 Size: 219 1695 993 1135 681 191 1471 Fragments arranged by size: 1695 1471 1135 993 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6385 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6385 SmaI CCC'GGG Cuts at: 1872 3712 1872 Size: 1840 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3248 4489 5390 5652 820 Size: 197 926 1305 1241 901 262 1553 Fragments arranged by size: 1553 1305 1241 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4176 493 Size: 3683 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6385 SphI G_CATG'C Cuts at: 1132 2645 3743 1132 Size: 1513 1098 3774 Fragments arranged by size: 3774 1513 1098 SspI AAT'ATT Cuts at: 5 52 4309 5 Size: 47 4257 2081 Fragments arranged by size: 4257 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6385 StyI C'CwwG_G Cuts at: 513 1065 2275 2716 2981 3824 513 Size: 552 1210 441 265 843 3074 Fragments arranged by size: 3074 1210 843 552 441 265 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2994 3061 3641 4412 4429 4582 4767 Size: 1255 1414 67 580 771 17 153 185 Cuts at: 4767 5212 325 Size: 445 1498 Fragments arranged by size: 1498 1414 1255 771 580 445 185 153 67 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3195 4631 6343 Size: 274 80 33 51 1443 1218 1436 1712 Cuts at: 6343 96 Size: 138 Fragments arranged by size: 1712 1443 1436 1218 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2548 2580 6345 6377 2548 Size: 32 3765 32 2556 Fragments arranged by size: 3765 2556 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6385 XbaI T'CTAG_A Cuts at: 3280 3721 3280 Size: 441 5944 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3044 3527 3044 Size: 483 5902 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6385 XmaI C'CCGG_G Cuts at: 1870 3710 1870 Size: 1840 4545 XmnI GAAnn'nnTTC Cuts at: 3156 3188 4514 3156 Size: 32 1326 5027 Fragments arranged by size: 5027 1326 32 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4193 276 Size: 53 83 186 936 2659 2468 Fragments arranged by size: 2659 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI