################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Alw44I 189 GTGCAC G^TGCAC ApaLI 189 GTGCAC G^TGCAC AspHI 189 GTGCAC G[AT]GC[AT]^C BamHI 63 GGATCC G^GATCC BclI 144 TGATCA T^GATCA BfaI 194 CTAG C^TAG BglI 69 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 609 AGATCT A^GATCT BmyI 189 GTGCAC G[AGT]GC[ACT]^C BsaAI 496 TACGTG [CT]AC^GT[GA] BsiHKAI 189 GTGCAC G[AT]GC[AT]^C BsiYI 359 CCCATAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 359 CCCATAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 189 GTGCAC G[AGT]GC[ACT]^C BspHI 132 TCATGA T^CATGA BspMI 390 GCAGGT ACCTGC(4/8) BsrDI 615 CATTGC GCAATG(2/0) BssKI 285 CCAGG ^CC[ACGT]GG BstAPI 205 GCAGAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 491 GGATG GGATG(2/0) BstNI 285 CCAGG CC^[AT]GG Cac8I 74 GCTGGC GC[ACGT]^[ACGT]GC DrdI 4 GACCCCGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRII 285 CCAGG ^CC[AT]GG FbaI 144 TGATCA T^GATCA FokI 491 GGATG GGATG(9/13) MaeI 194 CTAG C^TAG MspA1I 290 CAGCTG C[AC]G^C[GT]G MvaI 285 CCAGG CC^[AT]GG PstI 559 CTGCAG CTGCA^G PvuII 290 CAGCTG CAG^CTG RcaI 132 TCATGA T^CATGA ScaI 110 AGTACT AGT^ACT ScrFI 285 CCAGG CC^[ACGT]GG SfcI 559 CTGCAG C^T[GA][CT]AG SmlI 15 CTTGAG C^T[CT][GA]AG StuI 515 AGGCCT AGG^CCT Tsp45I 172 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 32 GTCTAC GT^[AC][GT]AC 319 GTCTAC AciI 67 CCGC CCGC(-3/-1) 39 GCGG AfaI 111 GTAC GT^AC 495 GTAC AlwI 64 GATCC GGATC(4/5) 63 GGATC AvaII 3 GGACC G^G[AT]CC 234 GGACC BsmAI 431 GAGAC GTCTC(1/5) 52 GTCTC BstXI 87 CCATATTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 285 CCAGGCAGCTGG BstYI 63 GGATCC [GA]^GATC[CT] 609 AGATCT Cfr13I 3 GGACC G^G[ACGT]CC 234 GGACC EarI 451 GAAGAG CTCTTC(1/4) 591 GAAGAG HaeIII 441 GGCC GG^CC 516 GGCC HinfI 355 GATTC G^A[ACGT]TC 550 GAATC HphI 173 TCACC GGTGA(8/7) 569 TCACC Ksp632I 451 GAAGAG CTCTTC(1/4) 591 GAAGAG MaeII 149 ACGT A^CGT 497 ACGT MboII 451 GAAGA GAAGA(8/7) 591 GAAGA MflI 63 GGATCC [GA]^GATC[CT] 609 AGATCT RsaI 111 GTAC GT^AC 495 GTAC Sau96I 3 GGACC G^G[ACGT]CC 234 GGACC TfiI 355 GATTC G^A[AT]TC 550 GAATC XhoII 63 GGATCC [GA]^GATC[CT] 609 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AluI BbvI BpmI BsrI DdeI DpnII Fnu4HI ItaI MaeIII MboI MnlI MwoI NdeII NlaIII NlaIV Sau3AI TseI TspRI