################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KpnI MamI MfeI MluI MluNI MroI MscI MunI NarI NcoI NdeI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 903 CGCG CG^CG AcsI 1306 AAATTT [GA]^AATT[CT] AocI 58 CCTCAGG CC^T[ACGT]AGG ApaI 510 GGGCCC GGGCC^C ApoI 1306 AAATTT [GA]^AATT[CT] AspHI 961 GAGCTC G[AT]GC[AT]^C BanI 298 GGCACC G^G[CT][GA]CC BbsI 1378 GAAGAC GAAGAC(2/6) BbvCI 922 CCTCAGC CCTCAGC(-5/-2) BcnI 879 CCGGG CC^[GC]GG BlpI 958 GCTGAGC GC^T[ACGT]AGC BpmI 862 CTCCAG CTGGAG(16/14) Bpu1102I 958 GCTGAGC GC^T[ACGT]AGC BpuAI 1378 GAAGAC GAAGAC(2/6) BsgI 240 CTGCAC GTGCAG(16/14) BsiHKAI 961 GAGCTC G[AT]GC[AT]^C BsrFI 602 GCCGGC [GA]^CCGG[CT] BsrI 1194 CCAGT ACTGG(1/-1) BstAPI 1140 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 903 CGCG CG^CG BstXI 455 CCAATGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 58 CCTCAGG CC^T[ACGT]AGG CelII 958 GCTGAGC GC^T[ACGT]AGC CfoI 896 GCGC GCG^C Cfr10I 602 GCCGGC [GA]^CCGG[CT] DsaI 902 CCGCGG C^C[GA][CT]GG Eco81I 58 CCTCAGG CC^T[ACGT]AGG EcoNI 406 CCTGTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HhaI 896 GCGC GCG^C HinP1I 896 GCGC G^CGC HincII 352 GTCAAC GT[CT]^[GA]AC HindII 352 GTCAAC GT[CT]^[GA]AC KspI 902 CCGCGG CCGC^GG MvnI 903 CGCG CG^CG NaeI 602 GCCGGC GCC^GGC NciI 879 CCGGG CC^[GC]GG NgoMIV 602 GCCGGC G^CCGGC NheI 429 GCTAGC G^CTAGC PflMI 385 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 234 CAGCTG CAG^CTG SacI 961 GAGCTC GAGCT^C SacII 902 CCGCGG CCGC^GG ScaI 1154 AGTACT AGT^ACT StuI 1095 AGGCCT AGG^CCT Van91I 385 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1218 CCACTGCCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AfaI 546 GTAC GT^AC 1155 GTAC AlwI 63 GGATC GGATC(4/5) 680 GGATC AvaI 766 CTCGAG C^[CT]CG[GA]G 909 CTCGAG AvaII 416 GGACC G^G[AT]CC 1014 GGACC BanII 510 GGGCCC G[GA]GC[CT]^C 961 GAGCTC BmyI 510 GGGCCC G[AGT]GC[ACT]^C 961 GAGCTC BsmBI 8 CGTCTC CGTCTC(1/5) 1435 GAGACG BsoBI 766 CTCGAG C^[CT]CG[GA]G 909 CTCGAG Bsp1286I 510 GGGCCC G[AGT]GC[ACT]^C 961 GAGCTC BsrBI 204 CCGCTC CCGCTC(-3/-3) 1239 CCGCTC BsrDI 288 CATTGC GCAATG(2/0) 995 GCAATG BstYI 63 GGATCT [GA]^GATC[CT] 680 GGATCT EarI 777 GAAGAG CTCTTC(1/4) 1162 GAAGAG FauI 203 CCCGC CCCGC(4/6) 1238 CCCGC HapII 603 CCGG C^CGG 879 CCGG HpaII 603 CCGG C^CGG 879 CCGG Ksp632I 777 GAAGAG CTCTTC(1/4) 1162 GAAGAG MflI 63 GGATCT [GA]^GATC[CT] 680 GGATCT MspI 603 CCGG C^CGG 879 CCGG PaeR7I 766 CTCGAG C^TCGAG 909 CTCGAG PpuMI 415 GGGACCC [GA]G^G[AT]CC[CT] 1013 AGGACCT PstI 528 CTGCAG CTGCA^G 613 CTGCAG RsaI 546 GTAC GT^AC 1155 GTAC TfiI 168 GAATC G^A[AT]TC 1406 GAATC XhoI 766 CTCGAG C^TCGAG 909 CTCGAG XhoII 63 GGATCT [GA]^GATC[CT] 680 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AluI AlwNI BbvI BfaI BsaJI BsiYI BslI BsmAI BsmFI BspMI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I