(Circular) (Six-base) MAPSORT of: pFN21AE1562.seq Check: 3852 from: 1 to: 6151 pFN21AE1562 6151 bp With 182 enzymes: SgfI * August 16, 2010 14:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2345 2511 2345 Size: 166 5985 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3961 278 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3494 1937 Size: 1557 4594 Acc65I G'GTAC_C Cuts at: 3472 3472 Size: 6151 AclI AA'CG_TT Cuts at: 1563 4278 4651 1563 Size: 2715 373 3063 Fragments arranged by size: 3063 2715 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2801 4214 5598 5866 1632 Size: 1169 1413 1384 268 1917 Fragments arranged by size: 1917 1413 1384 1169 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5954 AflIII A'CryG_T Cuts at: 1203 5050 6027 1203 Size: 3847 977 1327 Fragments arranged by size: 3847 1327 977 AgeI A'CCGG_T Cuts at: 4982 4982 Size: 6151 AhdI GACnn_n'nnGTC Cuts at: 4880 4880 Size: 6151 AleI CACnn'nnGTG Cuts at: 1166 2153 3201 1166 Size: 987 1048 4116 Fragments arranged by size: 4116 1048 987 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6151 AlwNI CAG_nnn'CTG Cuts at: 2244 3022 3040 3131 3151 3894 5466 2244 Size: 778 18 91 20 743 1572 2929 Fragments arranged by size: 2929 1572 778 743 91 20 18 ApaI G_GGCC'C Cuts at: 2519 2519 Size: 6151 ApaLI G'TGCA_C Cuts at: 4208 5364 4208 Size: 1156 4995 ApoI r'AATT_y Cuts at: 1493 3311 3460 3686 3838 5947 1493 Size: 1818 149 226 152 2109 1697 Fragments arranged by size: 2109 1818 1697 226 152 149 AseI AT'TA_AT Cuts at: 160 1032 4705 160 Size: 872 3673 1606 Fragments arranged by size: 3673 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 AvaI C'yCGr_G Cuts at: 1870 1943 2771 2914 3476 1870 Size: 73 828 143 562 4545 Fragments arranged by size: 4545 828 562 143 73 BamHI G'GATC_C Cuts at: 3481 3848 3481 Size: 367 5784 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2303 3472 4927 618 Size: 325 217 627 516 1169 1455 1842 Fragments arranged by size: 1842 1455 1169 627 516 325 217 BanII G_rGCy'C Cuts at: 1391 2519 2970 3470 1391 Size: 1128 451 500 4072 Fragments arranged by size: 4072 1128 500 451 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3390 928 Size: 971 1491 3689 Fragments arranged by size: 3689 1491 971 BbvCI CC'TCA_GC Cuts at: 2928 2928 Size: 6151 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4342 4376 1903 Size: 34 50 34 2321 34 3678 Fragments arranged by size: 3678 2321 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4043 5253 4043 Size: 1210 4941 BclI T'GATC_A Cuts at: 1548 1548 Size: 6151 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4762 136 Size: 107 122 71 631 605 3090 1525 Fragments arranged by size: 3090 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6146 6146 Size: 6151 BlpI GC'TnA_GC Cuts at: 2964 3568 2964 Size: 604 5547 Bme1580I G_kGCm'C Cuts at: 1790 2519 4212 5368 1790 Size: 729 1693 1156 2573 Fragments arranged by size: 2573 1693 1156 729 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6151 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3882 4840 442 Size: 416 53 83 399 24 2465 958 1753 Fragments arranged by size: 2465 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2438 1056 Size: 1382 4769 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2852 4811 1239 Size: 1613 1959 2579 Fragments arranged by size: 2579 1959 1613 Bpu10I CC'TnA_GC Cuts at: 2928 3015 2928 Size: 87 6064 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2147 4276 5141 5439 2147 Size: 2129 865 298 2859 Fragments arranged by size: 2859 2129 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4814 882 Size: 617 3315 2219 Fragments arranged by size: 3315 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3942 493 Size: 711 2738 2702 Fragments arranged by size: 2738 2702 711 BsaBI GATnn'nnATC Cuts at: 6145 6145 Size: 6151 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3958 4340 4975 275 Size: 53 83 186 936 2425 382 635 1451 Fragments arranged by size: 2425 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4584 4982 5256 5403 5814 4584 Size: 398 274 147 411 4921 Fragments arranged by size: 4921 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5926 Fragments arranged by size: 5926 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6151 BseYI C'CCAG_C Cuts at: 2751 3233 5354 5850 2751 Size: 482 2121 496 3052 Fragments arranged by size: 3052 2121 496 482 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2230 1163 Size: 573 494 5084 Fragments arranged by size: 5084 573 494 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3601 4362 5390 1540 Size: 275 186 1600 761 1028 2301 Fragments arranged by size: 2301 1600 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2970 4212 4297 5368 2970 Size: 1242 85 1071 3753 Fragments arranged by size: 3753 1242 1071 85 BsmI GAATG_Cn' Cuts at: 3662 3755 3662 Size: 93 6058 BsmBI CGTCTCn'nnnn_ Cuts at: 2019 3434 2019 Size: 1415 4736 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2519 2970 3470 4212 4297 5368 1391 Size: 399 729 451 500 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 729 500 451 399 85 BspEI T'CCGG_A Cuts at: 5814 5814 Size: 6151 BspHI T'CATG_A Cuts at: 4040 5770 4040 Size: 1730 4421 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2345 2511 3159 3506 844 Size: 1501 166 648 347 3489 Fragments arranged by size: 3489 1501 648 347 166 BsrBI CCG'CTC Cuts at: 1394 1823 2211 3246 4038 1394 Size: 429 388 1035 792 3507 Fragments arranged by size: 3507 1035 792 429 388 BsrDI GCAATG_nn' Cuts at: 65 1248 2292 3007 4646 4820 65 Size: 1183 1044 715 1639 174 1396 Fragments arranged by size: 1639 1396 1183 1044 715 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2607 4795 4982 1700 Size: 110 797 2188 187 2869 Fragments arranged by size: 2869 2188 797 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6151 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6151 BssSI C'ACGA_G Cuts at: 4211 5223 4211 Size: 1012 5139 BstAPI GCAn_nnn'nTGC Cuts at: 794 3151 794 Size: 2357 3794 BstBI TT'CG_AA Cuts at: 4955 4955 Size: 6151 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6151 BstXI CCAn_nnnn'nTGG Cuts at: 2467 2467 Size: 6151 BstYI r'GATC_y Cuts at: 1972 2068 2685 3481 3848 4233 4250 5702 5788 Size: 96 617 796 367 385 17 1452 86 Cuts at: 5788 5800 6146 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 796 617 385 367 346 96 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2064 2064 Size: 6151 BtgI C'CryG_G Cuts at: 513 1065 2907 513 Size: 552 1842 3757 Fragments arranged by size: 3757 1842 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2125 3247 487 Size: 683 56 899 1122 3391 Fragments arranged by size: 3391 1122 899 683 56 BtsI GCAGTG_nn' Cuts at: 2239 2313 3223 3667 4461 4481 2239 Size: 74 910 444 794 20 3909 Fragments arranged by size: 3909 910 794 444 74 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6151 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2134 2169 2382 2417 529 Size: 35 1570 35 213 35 4263 Fragments arranged by size: 4263 1570 213 35 35 35 DraI TTT'AAA Cuts at: 3455 3455 Size: 6151 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5158 809 Size: 507 3842 1802 Fragments arranged by size: 3842 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3598 4487 6116 Size: 54 1425 50 275 18 1768 889 1629 Cuts at: 6116 8 Size: 43 Fragments arranged by size: 1768 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3598 1537 Size: 275 1786 4090 Fragments arranged by size: 4090 1786 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2777 3162 4081 1353 Size: 1424 385 919 3423 Fragments arranged by size: 3423 1424 919 385 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2626 3238 4738 5112 5258 387 Size: 1064 138 1037 612 1500 374 146 1280 Fragments arranged by size: 1500 1280 1064 1037 612 374 146 138 EcoICRI GAG'CTC Cuts at: 2968 2968 Size: 6151 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2801 2852 4214 4811 5598 5866 1239 Size: 393 1169 51 1362 597 787 268 1524 Fragments arranged by size: 1524 1362 1169 787 597 393 268 51 EcoNI CCTnn'n_nnAGG Cuts at: 2415 2415 Size: 6151 EcoO109I rG'GnC_Cy Cuts at: 2421 2515 2516 2538 3019 2421 Size: 94 1 22 481 5553 Fragments arranged by size: 5553 481 94 22 1 EcoRI G'AATT_C Cuts at: 3460 3838 3460 Size: 378 5773 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2704 2837 2704 Size: 133 6018 FspI TGC'GCA Cuts at: 4657 4657 Size: 6151 HaeII r_GCGC'y Cuts at: 1678 5298 1678 Size: 3620 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3321 3353 4792 4824 4866 Size: 32 135 32 1749 32 1439 32 42 Cuts at: 4866 4898 1373 Size: 32 2626 Fragments arranged by size: 2626 1749 1439 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2359 3495 3741 669 Size: 988 281 421 1136 246 3079 Fragments arranged by size: 3079 1136 988 421 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6151 HpaI GTT'AAC Cuts at: 3741 3741 Size: 6151 KpnI G_GTAC'C Cuts at: 3476 3476 Size: 6151 MfeI C'AATT_G Cuts at: 3750 3750 Size: 6151 MluI A'CGCG_T Cuts at: 6027 6027 Size: 6151 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2083 5265 5449 1305 Size: 778 3182 184 2007 Fragments arranged by size: 3182 2007 778 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4329 Fragments arranged by size: 4329 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2153 3201 3287 4109 4468 4627 518 Size: 648 987 1048 86 822 359 159 2042 Fragments arranged by size: 2042 1048 987 822 648 359 159 86 MspA1I CmG'CkG Cuts at: 1606 2199 2241 2300 2909 3037 3567 4244 5392 Size: 593 42 59 609 128 530 677 1148 Cuts at: 5392 5637 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 609 593 530 245 128 59 42 NaeI GCC'GGC Cuts at: 2609 2609 Size: 6151 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5599 NdeI CA'TA_TG Cuts at: 387 387 Size: 6151 NgoMIV G'CCGG_C Cuts at: 2607 2607 Size: 6151 NheI G'CTAG_C Cuts at: 1052 2434 1052 Size: 1382 4769 NotI GC'GGCC_GC Cuts at: 3598 3598 Size: 6151 NspI r_CATG'y Cuts at: 1132 1229 3509 5054 1132 Size: 97 2280 1545 2229 Fragments arranged by size: 2280 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6151 PciI A'CATG_T Cuts at: 5050 5050 Size: 6151 PflMI CCAn_nnn'nTGG Cuts at: 1108 2396 1108 Size: 1288 4863 PmeI GTTT'AAAC Cuts at: 3455 3455 Size: 6151 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4220 4252 5733 5765 4220 Size: 32 1481 32 4606 Fragments arranged by size: 4606 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2421 3019 2421 Size: 598 5553 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6151 PsiI TTA'TAA Cuts at: 3309 3721 3309 Size: 412 5739 PspOMI G'GGCC_C Cuts at: 2515 2515 Size: 6151 PspXI vC'TCGA_Gb Cuts at: 2771 2914 2771 Size: 143 6008 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3035 3067 3035 Size: 32 6119 PstI C_TGCA'G Cuts at: 830 2537 2622 3503 830 Size: 1707 85 881 3478 Fragments arranged by size: 3478 1707 881 85 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6151 PvuII CAG'CTG Cuts at: 2241 2241 Size: 6151 SacI G_AGCT'C Cuts at: 2970 2970 Size: 6151 SacII CC_GC'GG Cuts at: 2910 2910 Size: 6151 SalI G'TCGA_C Cuts at: 1936 3493 1936 Size: 1557 4594 SanDI GG'GwC_CC Cuts at: 2421 2421 Size: 6151 SbfI CC_TGCA'GG Cuts at: 3503 3503 Size: 6151 ScaI AGT'ACT Cuts at: 3161 4399 6111 3161 Size: 1238 1712 3201 Fragments arranged by size: 3201 1712 1238 SfcI C'TryA_G Cuts at: 826 1045 2533 2618 2656 3499 4634 5315 5506 Size: 219 1488 85 38 843 1135 681 191 Cuts at: 5506 826 Size: 1471 Fragments arranged by size: 1488 1471 1135 843 681 219 191 85 38 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6151 SmaI CCC'GGG Cuts at: 1872 3478 1872 Size: 1606 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2162 2771 2914 4255 5156 5418 Size: 197 926 219 609 143 1341 901 262 Cuts at: 5418 820 Size: 1553 Fragments arranged by size: 1553 1341 926 901 609 262 219 197 143 SnaBI TAC'GTA Cuts at: 493 3942 493 Size: 3449 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6151 SphI G_CATG'C Cuts at: 1132 3509 1132 Size: 2377 3774 SspI AAT'ATT Cuts at: 5 52 4075 5 Size: 47 4023 2081 Fragments arranged by size: 4023 2081 47 StuI AGG'CCT Cuts at: 1517 3102 1517 Size: 1585 4566 StyI C'CwwG_G Cuts at: 513 1065 2098 2569 2995 3590 513 Size: 552 1033 471 426 595 3074 Fragments arranged by size: 3074 1033 595 552 471 426 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4178 4195 4348 4533 4978 325 Size: 1255 2598 17 153 185 445 1498 Fragments arranged by size: 2598 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2550 3159 4397 Size: 274 80 33 51 1443 573 609 1238 Cuts at: 4397 6109 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1238 609 573 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3061 3093 3396 3428 6111 6143 3061 Size: 32 303 32 2683 32 3069 Fragments arranged by size: 3069 2683 303 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6151 XbaI T'CTAG_A Cuts at: 3487 3487 Size: 6151 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3230 3230 Size: 6151 XhoI C'TCGA_G Cuts at: 1943 2771 2914 1943 Size: 828 143 5180 Fragments arranged by size: 5180 828 143 XmaI C'CCGG_G Cuts at: 1870 3476 1870 Size: 1606 4545 XmnI GAAnn'nnTTC Cuts at: 4280 4280 Size: 6151 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3959 276 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI RsrII SapI SexAI SfiI SfoI SrfI SwaI